Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,786 G→T 100% E42* (GAA→TAA)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,7860GT99.0% 659.6 / ‑1.8 204E42* (GAA→TAA) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (110/92);  total (111/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412641‑4412930
                                                                                                                                                 |                                                                                                                                                
tcTGTGGGGCTAAGGAGCGGATATGTTGTCCACAGAAATCCCGCTTGGCTTCTATGATAAAGGGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAATCGTTAGGCTTCGATTTGTTGGAAATGTCGGTAGATTaa                                                                                                                                                <  2:822175/147‑1 (MQ=255)
    tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                            <  2:1327565/148‑1 (MQ=255)
    tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                            <  1:227420/148‑1 (MQ=255)
    tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                            >  1:592529/1‑148 (MQ=255)
         cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGAt                                                                                                                                       <  2:1167815/148‑1 (MQ=255)
          tAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATc                                                                                                                                      >  1:2283121/1‑148 (MQ=255)
          tAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATc                                                                                                                                      >  1:182203/1‑148 (MQ=255)
            aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    <  1:1995239/148‑1 (MQ=255)
            aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    >  2:3069269/1‑148 (MQ=255)
             ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGTAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                   <  1:3114852/148‑1 (MQ=255)
             ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                   >  1:2860839/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  2:2604655/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  1:1070662/1‑148 (MQ=255)
               aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTg                                                                                                                                 >  1:2068534/1‑148 (MQ=255)
               aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTg                                                                                                                                 >  1:3119901/1‑148 (MQ=255)
               aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCCTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTg                                                                                                                                 <  1:2558622/148‑1 (MQ=255)
                gCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGt                                                                                                                                <  2:925199/148‑1 (MQ=255)
                    gtatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAATCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:451954/2‑148 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:2958489/1‑148 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:2246671/1‑148 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                           >  1:556674/1‑148 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGCAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                           >  1:1383731/1‑148 (MQ=255)
                        gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTc                                                                                                                        >  1:1108208/1‑148 (MQ=255)
                          tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCaa                                                                                                                      <  1:677787/148‑1 (MQ=255)
                             ccAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTg                                                                                                                   >  1:939282/1‑148 (MQ=255)
                              caaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTgg                                                                                                                  <  1:1269157/148‑1 (MQ=255)
                               aaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGa                                                                                                                 >  2:1261684/1‑148 (MQ=255)
                                aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                                <  1:3350837/148‑1 (MQ=255)
                                aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                                >  2:1067030/1‑148 (MQ=255)
                                 acaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGc                                                                                                               >  1:2242147/1‑148 (MQ=255)
                                   aaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcg                                                                                                             <  1:1062629/148‑1 (MQ=255)
                                     aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                           >  1:267383/1‑148 (MQ=255)
                                     aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                           >  2:1303727/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                         >  1:871298/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                         <  1:1634225/148‑1 (MQ=255)
                                           cTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagcagc                                                                                                     <  1:2698318/148‑1 (MQ=255)
                                            ttGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCg                                                                                                    <  1:3313954/148‑1 (MQ=255)
                                               gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                 <  2:1125083/148‑1 (MQ=255)
                                                 aTCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTgg                                                                                               >  2:1197124/1‑148 (MQ=255)
                                                  tCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                              >  1:2091264/1‑148 (MQ=255)
                                                   cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             >  2:2926358/1‑148 (MQ=255)
                                                   cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             <  2:403552/148‑1 (MQ=255)
                                                    tATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcgc                                                                                            <  1:1736542/148‑1 (MQ=255)
                                                    tATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcgc                                                                                            >  2:184317/1‑148 (MQ=255)
                                                     aTGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCt                                                                                           >  2:1969343/1‑148 (MQ=255)
                                                      tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                          <  1:1041217/148‑1 (MQ=255)
                                                       gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTgg                                                                                         >  1:2542270/1‑148 (MQ=255)
                                                        aaaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                                                                                        <  1:2174822/148‑1 (MQ=255)
                                                        aaaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                                                                                        >  1:1820881/1‑148 (MQ=255)
                                                        aaaaaGCGCGCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                                                                                        <  1:3140557/148‑1 (MQ=255)
                                                          aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                      >  2:426120/1‑148 (MQ=255)
                                                            aGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGACATGTCGGTAGATTAATCTGACGTTCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTGTGGCGCGGGTCAAt                                                                                    >  2:949074/1‑148 (MQ=255)
                                                              cgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGc                                                                                  >  1:821682/1‑148 (MQ=255)
                                                                cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa                                                                                <  2:3119900/148‑1 (MQ=255)
                                                                    ccgccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttgt                                                                            >  2:3391536/1‑148 (MQ=255)
                                                                       ccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa                                                                         <  2:2860839/148‑1 (MQ=255)
                                                                        cgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaa                                                                        <  1:1853343/148‑1 (MQ=255)
                                                                         ggggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa                                                                       <  2:2864336/148‑1 (MQ=255)
                                                                         ggggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa                                                                       <  2:1506830/148‑1 (MQ=255)
                                                                             aGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg                                                                   <  1:1173261/148‑1 (MQ=255)
                                                                                   ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcg                                                             >  2:2010084/1‑148 (MQ=255)
                                                                                   ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcg                                                             <  2:1448926/148‑1 (MQ=255)
                                                                                       ggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtg                                                         <  2:3277720/148‑1 (MQ=255)
                                                                                        gAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtgc                                                        >  2:3364321/1‑148 (MQ=255)
                                                                                        gAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtgc                                                        >  2:1717391/1‑148 (MQ=255)
                                                                                          aCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCg                                                      <  2:2597426/148‑1 (MQ=255)
                                                                                           cGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGt                                                     >  1:2915192/1‑148 (MQ=255)
                                                                                             ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTcc                                                   <  2:854988/148‑1 (MQ=255)
                                                                                             ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTcc                                                   <  2:854092/148‑1 (MQ=255)
                                                                                               tGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAt                                                 <  1:2583323/148‑1 (MQ=255)
                                                                                                   aCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGc                                             <  2:1820881/148‑1 (MQ=255)
                                                                                                   aCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGc                                             <  2:2111169/148‑1 (MQ=255)
                                                                                                    cTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGcc                                            <  1:616677/148‑1 (MQ=255)
                                                                                                     tGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCt                                           <  1:382510/148‑1 (MQ=255)
                                                                                                       gCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                         >  1:411841/1‑148 (MQ=255)
                                                                                                       gCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGCTAGATTAATCTGACGATCGCCTGTCGCGCCTCAACTGGCGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                         <  2:1487403/148‑1 (MQ=255)
                                                                                                           aaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                     >  1:1704658/1‑148 (MQ=255)
                                                                                                           aaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                     >  1:244042/1‑148 (MQ=255)
                                                                                                             aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                   <  1:2604647/148‑1 (MQ=255)
                                                                                                             aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                   >  2:221863/1‑148 (MQ=255)
                                                                                                             aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                   <  1:3473461/148‑1 (MQ=255)
                                                                                                               gTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAt                                 <  2:1383731/148‑1 (MQ=255)
                                                                                                                ttAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtc                                >  2:606058/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCCACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:2143133/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:288079/1‑148 (MQ=255)
                                                                                                                  aGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgt                              >  1:339165/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            >  2:3193609/1‑148 (MQ=255)
                                                                                                                      ttCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                          <  2:3131239/148‑1 (MQ=255)
                                                                                                                       tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAAGTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                         <  1:3224333/148‑1 (MQ=255)
                                                                                                                       tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                         >  2:2492373/1‑148 (MQ=255)
                                                                                                                          aTTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTccc                      >  1:2370727/1‑148 (MQ=255)
                                                                                                                            tttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGc                    >  1:2803857/1‑148 (MQ=255)
                                                                                                                            tttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGc                    <  2:1108208/148‑1 (MQ=255)
                                                                                                                            attGTCGAAATGACGGAAGATTAAACTGACGATCGCCTGTCGCGCCACAACTGGAGCCGCGAGCAGCGTCAGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCCTGTGCCTTTCTGCTCATCTTCGTTTccccc                    >  2:2915931/2‑146 (MQ=255)
                                                                                                                                tCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTggg                >  2:2772604/1‑148 (MQ=255)
                                                                                                                                 cGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc               >  1:176140/1‑148 (MQ=255)
                                                                                                                                   aaaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCACCTGGAGCCGCGAGCAGCGTCTGGCACTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAg             <  2:2311078/148‑1 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg           <  1:1869130/148‑1 (MQ=255)
                                                                                                                                        tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAg        >  2:2641677/1‑148 (MQ=255)
                                                                                                                                              gATTAACCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTGTCCCGCTGGGCAGTGAAGATGAcg  <  2:3003563/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412641‑4412930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑