BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,417,122	G→T	94.7%	R40S (CGT→AGT)	yjfZ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,417,122	0	G	T	94.7%	681.9 / 29.5	248	R40S (CGT→AGT)	yjfZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (3/10); new base T (119/115); total (123/125)
Fisher's exact test for biased strand distribution p-value = 8.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 249 total aligned reads displayed.

TCCATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTA  >  CP009273/4416975‑4417266

    tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                              >  2:1599179/1‑148 (MQ=255)

         aaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAg                                                                                                                                         <  2:3043559/148‑1 (MQ=255)

         aaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAg                                                                                                                                         <  1:2640658/148‑1 (MQ=255)

          aGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATATCAACAGTACGTCTGCCATAGCAATTATGATCTCTTCCACTATTTGAAAGt                                                                                                                                        >  2:315903/1‑148 (MQ=255)

           gAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTa                                                                                                                                       <  2:2344343/148‑1 (MQ=255)

            aaTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTaa                                                                                                                                      >  2:2074392/1‑148 (MQ=255)

            aaTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTaa                                                                                                                                      >  2:2920350/1‑148 (MQ=255)

             aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAAt                                                                                                                                     >  1:2316568/1‑148 (MQ=255)

               tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGt                                                                                                                                   >  1:1723153/1‑148 (MQ=255)

               tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGt                                                                                                                                   <  2:1556904/148‑1 (MQ=255)

               tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGt                                                                                                                                   <  1:247499/148‑1 (MQ=255)

                     cattcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                             <  1:2061958/148‑1 (MQ=255)

                       ttcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtg                                                                                                                           >  2:2530423/1‑148 (MQ=255)

                       ttcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtg                                                                                                                           >  2:527024/1‑148 (MQ=255)

                       ttcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAAAAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtg                                                                                                                           >  2:166804/1‑148 (MQ=255)

                        tcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtgc                                                                                                                          >  2:2336477/1‑148 (MQ=255)

                           ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTa                                                                                                                       <  1:1696998/148‑1 (MQ=255)

                            tgtgATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTAt                                                                                                                      <  2:2872476/148‑1 (MQ=255)

                              tgATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtg                                                                                                                    <  2:3165706/148‑1 (MQ=255)

                              tgATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtg                                                                                                                    <  2:1107189/148‑1 (MQ=255)

                                 ttCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtgatg                                                                                                                 <  1:3197961/148‑1 (MQ=255)

                                     aTCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaat                                                                                                             <  2:2914996/148‑1 (MQ=255)

                                     aTCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaat                                                                                                             >  2:859451/1‑148 (MQ=255)

                                         tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatttta                                                                                                         <  1:3566175/148‑1 (MQ=255)

                                           aGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaat                                                                                                       <  1:1145063/148‑1 (MQ=255)

                                             cATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaattt                                                                                                     >  1:2093429/1‑148 (MQ=255)

                                              aTTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatttt                                                                                                    <  2:2437483/148‑1 (MQ=255)

                                               ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAattttaatttta                                                                                                   <  2:2215857/148‑1 (MQ=255)

                                               ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAattttaatttta                                                                                                   >  2:2610647/1‑148 (MQ=255)

                                                 acacCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACt                                                                                                 <  2:3428309/148‑1 (MQ=255)

                                                  cacCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTg                                                                                                >  2:805084/1‑148 (MQ=255)

                                                   acCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTgg                                                                                               <  2:3534689/148‑1 (MQ=255)

                                                   acCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTgg                                                                                               <  1:1774710/148‑1 (MQ=255)

                                                       cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                           <  2:3431667/148‑1 (MQ=255)

                                                       cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                           <  2:1804510/148‑1 (MQ=255)

                                                       cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                           <  1:2617736/148‑1 (MQ=255)

                                                        gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                          >  2:36614/1‑148 (MQ=255)

                                                        gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                          <  1:689196/148‑1 (MQ=255)

                                                        gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                          <  1:2494303/148‑1 (MQ=255)

                                                         tcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTc                                                                                         <  1:2962573/148‑1 (MQ=255)

                                                          cgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGTTTTGTCg                                                                                        >  2:2278900/1‑148 (MQ=255)

                                                             tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTg                                                                                     >  2:457950/1‑148 (MQ=255)

                                                             tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTg                                                                                     >  1:1498566/1‑148 (MQ=255)

                                                              aaCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                    >  1:1253499/1‑148 (MQ=255)

                                                              aaCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                    <  2:1325712/148‑1 (MQ=255)

                                                               aCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGa                                                                                   >  2:3297335/1‑148 (MQ=255)

                                                               aCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGa                                                                                   >  1:778988/1‑148 (MQ=255)

                                                                       aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAAc                                                                           >  2:2907665/1‑148 (MQ=255)

                                                                       aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAAc                                                                           >  2:965570/1‑148 (MQ=255)

                                                                       aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAAc                                                                           >  2:1124736/1‑148 (MQ=255)

                                                                           cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                       >  1:1979500/1‑148 (MQ=255)

                                                                               ccAGTCTGTGAGATCTATTTTGTGGATATCAACATTACGTATGCCATAGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaaa                                                                   <  2:1354937/148‑1 (MQ=255)

                                                                               ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaaa                                                                   <  1:1876838/148‑1 (MQ=255)

                                                                                  gTCTGAGAGATCTAGTTTGTGGATCCCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAatat                                                                <  1:2300076/148‑1 (MQ=255)

                                                                                   tCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCTATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATg                                                               >  2:184159/1‑148 (MQ=255)

                                                                                    cTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGc                                                              <  2:1730618/148‑1 (MQ=255)

                                                                                     tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCa                                                             >  1:3529466/1‑148 (MQ=255)

                                                                                         agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                         >  2:1967789/1‑148 (MQ=255)

                                                                                         agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                         >  1:1053137/1‑148 (MQ=255)

                                                                                         agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                         <  1:2151654/148‑1 (MQ=255)

                                                                                         agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                         >  2:149478/1‑148 (MQ=255)

                                                                                         agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                         >  1:3412240/1‑148 (MQ=255)

                                                                                           aTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAAt                                                       <  1:967422/148‑1 (MQ=255)

                                                                                           aTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAAt                                                       >  1:541966/1‑148 (MQ=255)

                                                                                             cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGATATAAATATGCAGGAAATGa                                                     >  1:1912753/1‑148 (MQ=255)

                                                                                             cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                     <  1:2886098/148‑1 (MQ=255)

                                                                                             cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                     <  1:2442886/148‑1 (MQ=255)

                                                                                             cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                     <  1:2442905/148‑1 (MQ=255)

                                                                                               aGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaat                                                   >  1:825831/1‑148 (MQ=255)

                                                                                                 tttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataa                                                 >  1:2277422/1‑148 (MQ=255)

                                                                                                 tttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataa                                                 <  2:2767377/148‑1 (MQ=255)

                                                                                                  ttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaa                                                >  2:3267743/1‑148 (MQ=255)

                                                                                                  ttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaa                                                <  2:1810808/148‑1 (MQ=255)

                                                                                                    gtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                              <  1:3391254/148‑1 (MQ=255)

                                                                                                      ggATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGt                                            >  1:1756547/1‑148 (MQ=255)

                                                                                                         tctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGt                                         <  1:3151071/148‑1 (MQ=255)

                                                                                                           tcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTg                                       >  2:3104639/1‑148 (MQ=255)

                                                                                                            cAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTgg                                      >  1:379085/1‑148 (MQ=255)

                                                                                                            cAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTgg                                      <  2:376192/148‑1 (MQ=255)

                                                                                                              aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                    <  2:2500925/148‑1 (MQ=255)

                                                                                                               cAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTaa                                   <  1:859451/148‑1 (MQ=255)

                                                                                                                aGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAAt                                  >  1:2566367/1‑148 (MQ=255)

                                                                                                                 gTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATg                                 >  1:3488612/1‑148 (MQ=255)

                                                                                                                   aCGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTc                               >  2:2947226/1‑148 (MQ=255)

                                                                                                                      tCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCAtt                            >  2:2725744/1‑148 (MQ=255)

                                                                                                                         gCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTa                         <  2:3471082/148‑1 (MQ=255)

                                                                                                                         gCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTa                         <  1:457950/148‑1 (MQ=255)

                                                                                                                            aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                      <  1:1063920/148‑1 (MQ=255)

                                                                                                                             tCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGt                     >  2:2397541/1‑148 (MQ=255)

                                                                                                                               gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                   <  1:942287/148‑1 (MQ=255)

                                                                                                                               gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                   <  1:3078225/148‑1 (MQ=255)

                                                                                                                               gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                   <  1:2784256/148‑1 (MQ=255)

                                                                                                                                cAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                  <  1:2338256/148‑1 (MQ=255)

                                                                                                                                  aTTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCt                >  1:2833990/1‑148 (MQ=255)

                                                                                                                                       gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTa           >  1:68205/1‑148 (MQ=255)

                                                                                                                                       gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTAATGGTTTGTAGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGACTTTGGTAATGTCAGTGTAATTTTAGCTGTTTa           >  1:1533401/1‑148 (MQ=255)

                                                                                                                                            ctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtt      <  1:3193814/148‑1 (MQ=255)

                                                                                                                                              tCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACATGTTTAATGtttt    >  1:1207245/1‑148 (MQ=255)

                                                                                                                                                cACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTta  >  1:2579266/1‑148 (MQ=255)

                                                                                                                                                cACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTta  <  2:682917/148‑1 (MQ=255)

TCCATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTA  >  CP009273/4416975‑4417266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`