Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,445,719 A→T 100% T40S (ACC→TCC)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,445,7190AT96.0% 723.2 / 14.5 225T40S (ACC→TCC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (3/6);  new base T (102/114);  total (105/120)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

GCGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445574‑4445863
                                                                                                                                                 |                                                                                                                                                
 cGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCt                                                                                                                                               >  2:2143574/1‑148 (MQ=255)
 cGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCt                                                                                                                                               <  1:310678/148‑1 (MQ=255)
  ggCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCtt                                                                                                                                              <  1:2725759/148‑1 (MQ=255)
  ggCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCtt                                                                                                                                              <  1:3270752/148‑1 (MQ=255)
   gCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTa                                                                                                                                             <  1:1962973/148‑1 (MQ=255)
      gATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACtt                                                                                                                                          >  2:2957154/1‑148 (MQ=255)
       aTTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTg                                                                                                                                         >  1:3347925/1‑148 (MQ=255)
        ttAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTga                                                                                                                                        >  1:716734/1‑148 (MQ=255)
        ttAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTga                                                                                                                                        <  1:156465/148‑1 (MQ=255)
        ttAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTga                                                                                                                                        >  1:1709399/1‑148 (MQ=255)
         tAAAAAAGGAGACTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTgag                                                                                                                                       <  1:975991/148‑1 (MQ=255)
             aaaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGc                                                                                                                                   <  2:2505874/148‑1 (MQ=255)
              aaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCa                                                                                                                                  <  2:3297114/148‑1 (MQ=255)
              aaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCa                                                                                                                                  <  1:3104894/148‑1 (MQ=255)
              aaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCa                                                                                                                                  >  2:1574451/1‑148 (MQ=255)
              aaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCa                                                                                                                                  <  2:1525104/148‑1 (MQ=255)
               aGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCaa                                                                                                                                 <  1:1570820/148‑1 (MQ=255)
               aGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCaa                                                                                                                                 >  1:3005121/1‑148 (MQ=255)
                ggATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAg                                                                                                                                >  1:1202908/1‑148 (MQ=255)
                ggATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAg                                                                                                                                >  2:1220415/1‑148 (MQ=255)
                   tAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCa                                                                                                                             <  1:3052764/148‑1 (MQ=255)
                    aGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCAt                                                                                                                            <  1:3099460/148‑1 (MQ=255)
                    aGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCAt                                                                                                                            <  1:2337170/148‑1 (MQ=255)
                      tGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGTGCGGTCTGGCGATGCTGGCGCGCAAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTg                                                                                                                          >  1:1779258/1‑148 (MQ=255)
                      tGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTg                                                                                                                          <  1:867590/148‑1 (MQ=255)
                          gTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCt                                                                                                                      >  2:2224380/1‑148 (MQ=255)
                           tATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     <  1:840194/148‑1 (MQ=255)
                           tATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     <  2:1106959/148‑1 (MQ=255)
                           tATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     <  1:2129280/148‑1 (MQ=255)
                            aTGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGa                                                                                                                    <  2:1199972/148‑1 (MQ=255)
                                gcATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAATCAAGGCATTGAGCTGATTCa                                                                                                                <  1:2029098/148‑1 (MQ=255)
                                   ttCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAggg                                                                                                             >  1:629879/1‑148 (MQ=255)
                                    tCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAgggg                                                                                                            >  2:2123978/1‑148 (MQ=255)
                                     cATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGt                                                                                                           <  2:1739550/148‑1 (MQ=255)
                                     cATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGt                                                                                                           >  2:2030225/1‑148 (MQ=255)
                                     cATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGt                                                                                                           <  1:1087109/148‑1 (MQ=255)
                                       tatTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTAc                                                                                                         >  2:690398/1‑148 (MQ=255)
                                        atTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACg                                                                                                        <  2:897517/148‑1 (MQ=255)
                                        atTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACg                                                                                                        <  1:465347/148‑1 (MQ=255)
                                          tttAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGAt                                                                                                      >  1:75521/1‑148 (MQ=255)
                                              ggAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgcca                                                                                                  >  2:356296/1‑148 (MQ=255)
                                               gAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccag                                                                                                 >  1:1404439/1‑148 (MQ=255)
                                                   ttGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGccagccag                                                                                             >  2:1482324/1‑148 (MQ=255)
                                                     gtgGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCt                                                                                           <  2:1315009/148‑1 (MQ=255)
                                                      tgGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTc                                                                                          >  2:2609040/1‑148 (MQ=255)
                                                        gCACATTTATGGGCGGTCTGGCGCTGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     >  1:418497/1‑119 (MQ=255)
                                                        gCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     <  2:418497/119‑1 (MQ=255)
                                                        gCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGa                                                                                        >  2:2126909/1‑148 (MQ=255)
                                                          acaTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGGTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGc                                                                                      <  1:2692168/148‑1 (MQ=255)
                                                           caTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGcc                                                                                     >  2:1893138/1‑148 (MQ=255)
                                                             tttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGc                                                                                   >  1:1701668/1‑148 (MQ=255)
                                                              ttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCa                                                                                  >  2:1448332/1‑148 (MQ=255)
                                                               tATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAg                                                                                 <  1:980706/148‑1 (MQ=255)
                                                                   ggCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCgg                                                                             >  1:2856594/1‑148 (MQ=255)
                                                                     cGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAt                                                                           <  1:2506555/148‑1 (MQ=255)
                                                                       gTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCt                                                                         <  2:2375742/148‑1 (MQ=255)
                                                                         cTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTgg                                                                       <  2:3463845/148‑1 (MQ=255)
                                                                           ggCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTg                                                                     >  1:2306400/1‑148 (MQ=255)
                                                                            gCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGa                                                                    >  2:777329/1‑148 (MQ=255)
                                                                             cGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGat                                                                   <  2:127019/148‑1 (MQ=255)
                                                                               aTGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatta                                                                 >  1:2421385/1‑148 (MQ=255)
                                                                               aTGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatta                                                                 <  1:724144/148‑1 (MQ=255)
                                                                                 gCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattatt                                                               <  2:1635675/148‑1 (MQ=255)
                                                                                          ccAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGcc                                                      <  1:1906850/148‑1 (MQ=255)
                                                                                            aGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCAt                                                    >  2:728261/1‑148 (MQ=255)
                                                                                             gTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATg                                                   >  2:1380461/1‑148 (MQ=255)
                                                                                              ttAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGa                                                  >  2:590078/1‑148 (MQ=255)
                                                                                               tAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGAc                                                 >  2:1259824/1‑148 (MQ=255)
                                                                                                 ggCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATTAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGAccc                                               >  1:1749887/1‑148 (MQ=255)
                                                                                                   ccATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGt                                             <  2:3372332/148‑1 (MQ=255)
                                                                                                   ccATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGt                                             >  2:2312709/1‑148 (MQ=255)
                                                                                                    cATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTg                                            <  1:2032078/148‑1 (MQ=255)
                                                                                                     aTGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTgg                                           >  2:1464799/1‑148 (MQ=255)
                                                                                                      tGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGa                                          >  2:515350/1‑148 (MQ=255)
                                                                                                       gAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaa                                         <  1:2758582/148‑1 (MQ=255)
                                                                                                       gAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaa                                         >  1:2881913/1‑148 (MQ=255)
                                                                                                        aaGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaaa                                        >  1:1040180/1‑148 (MQ=255)
                                                                                                               gggTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgt                                 <  2:2259656/148‑1 (MQ=255)
                                                                                                                     ggACTCCATTGTGTATCCGCCGATGTGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGAACCGTGGAAATCCGTGTGTGGaa                           <  2:221400/148‑1 (MQ=255)
                                                                                                                     ggACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGaa                           >  2:1696926/1‑148 (MQ=255)
                                                                                                                      gACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAg                          >  2:3063748/1‑148 (MQ=255)
                                                                                                                      gACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCAGTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAGCTGGGGATTAGTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAg                          >  2:1990251/1‑148 (MQ=255)
                                                                                                                       aCGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGc                         >  1:3167163/1‑148 (MQ=255)
                                                                                                                       aCGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGc                         >  2:310789/1‑148 (MQ=255)
                                                                                                                       aCGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGc                         <  2:2567314/148‑1 (MQ=255)
                                                                                                                         gCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCgg                       <  2:2294733/148‑1 (MQ=255)
                                                                                                                             aTGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACt                   <  2:3005121/148‑1 (MQ=255)
                                                                                                                             aTGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAACCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACt                   <  2:2516585/148‑1 (MQ=255)
                                                                                                                               gtgtATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTgg                 >  2:370150/1‑148 (MQ=255)
                                                                                                                                    tCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaa            >  1:3210513/1‑148 (MQ=255)
                                                                                                                                     ccgccgATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaa           <  1:1990292/148‑1 (MQ=255)
                                                                                                                                       gccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAat         <  2:1966743/148‑1 (MQ=255)
                                                                                                                                         cgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAatat       <  2:3529904/148‑1 (MQ=255)
                                                                                                                                         cgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAatat       <  2:2641135/148‑1 (MQ=255)
                                                                                                                                           aTGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATcc     >  2:750856/1‑148 (MQ=255)
                                                                                                                                            tGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATccc    <  1:2224380/148‑1 (MQ=255)
                                                                                                                                            tGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATccc    <  2:716428/148‑1 (MQ=255)
                                                                                                                                             gAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCt   <  1:2609040/148‑1 (MQ=255)
                                                                                                                                              aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  <  2:434472/148‑1 (MQ=255)
                                                                                                                                              aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  >  2:2705536/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445574‑4445863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑