Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,520,701 G→T 100% G256G (GGC→GGA)  sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,520,7010GT98.5% 646.1 / 0.7 202G256G (GGC→GGA) sgcXputative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (96/103);  total (98/104)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

CTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGA  >  CP009273/4520556‑4520844
                                                                                                                                                 |                                                                                                                                               
cTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTcc                                                                                                                                               <  2:2683547/148‑1 (MQ=255)
cTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTcc                                                                                                                                               >  2:1053170/1‑148 (MQ=255)
 tGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTccc                                                                                                                                              <  1:1227627/148‑1 (MQ=255)
   ttATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTg                                                                                                                                            >  2:771330/1‑148 (MQ=255)
   ttATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTg                                                                                                                                            >  2:771299/1‑148 (MQ=255)
   ttATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTg                                                                                                                                            <  1:753663/148‑1 (MQ=255)
     aTCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGAt                                                                                                                                          <  1:135560/148‑1 (MQ=255)
        aCGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTaa                                                                                                                                       <  1:1981398/148‑1 (MQ=255)
          gCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATc                                                                                                                                     <  2:3560461/148‑1 (MQ=255)
               gAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGAc                                                                                                                                <  2:3516548/148‑1 (MQ=255)
               gAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGAc                                                                                                                                <  1:810240/148‑1 (MQ=255)
                 gCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCt                                                                                                                              <  1:29788/148‑1 (MQ=255)
                    aCTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCgg                                                                                                                           <  2:2849310/148‑1 (MQ=255)
                      ttCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGaa                                                                                                                         <  1:2775497/148‑1 (MQ=255)
                      ttCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGaa                                                                                                                         <  1:671000/148‑1 (MQ=255)
                               cACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCgtgt                                                                                                                <  1:3336953/148‑1 (MQ=255)
                                    gAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATc                                                                                                           <  2:1979582/148‑1 (MQ=255)
                                    gAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATc                                                                                                           >  2:2071888/1‑148 (MQ=255)
                                    gAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATc                                                                                                           >  2:830538/1‑148 (MQ=255)
                                    gAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATc                                                                                                           >  1:1280591/1‑148 (MQ=255)
                                     aaTGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCa                                                                                                          <  1:2211661/148‑1 (MQ=255)
                                        gttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAggg                                                                                                       <  1:1465622/148‑1 (MQ=255)
                                         ttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgggg                                                                                                      >  2:1900336/1‑148 (MQ=255)
                                          tgtgTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGgt                                                                                                     >  2:1499728/1‑148 (MQ=255)
                                               tCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTcac                                                                                                >  2:1371779/1‑148 (MQ=255)
                                               tCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTcac                                                                                                <  1:1534332/148‑1 (MQ=255)
                                                  agagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGCCCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATACTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCacact                                                                                             >  1:3463495/1‑147 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCTGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                            <  2:2263136/148‑1 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                            >  1:3311913/1‑148 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                            >  1:2545953/1‑148 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                            >  1:2546235/1‑148 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                            >  1:2660563/1‑148 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                           >  2:2479606/1‑148 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                           <  1:3522986/148‑1 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                           >  2:3478156/1‑148 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATACTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                           >  1:2251641/1‑148 (MQ=255)
                                                        gTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAg                                                                                       <  2:3184337/148‑1 (MQ=255)
                                                         tCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGt                                                                                      >  2:3401646/1‑148 (MQ=255)
                                                         tCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGt                                                                                      >  1:2255305/1‑148 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                     <  2:2635409/148‑1 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                     <  2:1682518/148‑1 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                     <  1:2463431/148‑1 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                     <  1:50916/148‑1 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGGTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                     <  1:3419392/148‑1 (MQ=255)
                                                            gTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGat                                                                                   <  1:2888056/148‑1 (MQ=255)
                                                             ttCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGata                                                                                  >  2:1066921/1‑148 (MQ=255)
                                                                 aaCATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCaa                                                                              <  1:407695/148‑1 (MQ=255)
                                                                     tCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATAcc                                                                          >  1:1120430/1‑148 (MQ=255)
                                                                      ccGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCa                                                                         >  2:2886061/1‑148 (MQ=255)
                                                                      ccGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCa                                                                         >  2:2381224/1‑148 (MQ=255)
                                                                      ccGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCa                                                                         >  1:1787937/1‑148 (MQ=255)
                                                                        gTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAAt                                                                       <  1:1495258/148‑1 (MQ=255)
                                                                         tATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATc                                                                      >  2:1983861/1‑148 (MQ=255)
                                                                          aTCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCg                                                                     >  1:2628529/1‑148 (MQ=255)
                                                                            cAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGcc                                                                   >  1:2662710/1‑148 (MQ=255)
                                                                                cGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcaggt                                                               >  1:161995/1‑148 (MQ=255)
                                                                                   ggaggCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATa                                                                            >  1:1530172/1‑132 (MQ=255)
                                                                                    gaggCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcaggtcagg                                                           <  1:3068096/148‑1 (MQ=255)
                                                                                       gCGTGATTAATCCGGCCAGCGTTCCCCGAACATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGttt                                                        >  2:2178548/1‑148 (MQ=255)
                                                                                       gCGTGATTAATCCGGCCAGCGTTCCCCGAACATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGGCAGGttt                                                        >  2:2158714/1‑148 (MQ=255)
                                                                                          tGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTac                                                     <  1:3478156/148‑1 (MQ=255)
                                                                                           gATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTaca                                                    >  1:2494734/1‑148 (MQ=255)
                                                                                           gATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTaca                                                    <  1:55900/148‑1 (MQ=255)
                                                                                            aTTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTacac                                                   <  1:2000995/148‑1 (MQ=255)
                                                                                                  ccGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTa                                             <  1:2940449/148‑1 (MQ=255)
                                                                                                   cGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTaa                                            <  2:212757/148‑1 (MQ=255)
                                                                                                    ggCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAAt                                           <  1:2079620/148‑1 (MQ=255)
                                                                                                     gCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATa                                          <  2:492056/148‑1 (MQ=255)
                                                                                                       cAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATAct                                        >  1:2349571/1‑147 (MQ=255)
                                                                                                       cAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACg                                        <  2:73058/148‑1 (MQ=255)
                                                                                                        cgCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTAACACGGCGTAATACgg                                       >  1:2846583/2‑148 (MQ=255)
                                                                                                          cGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGa                                     >  1:2317519/1‑148 (MQ=255)
                                                                                                           ggtCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGaa                                    <  2:245021/146‑1 (MQ=255)
                                                                                                           gTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGaa                                    <  2:3035950/148‑1 (MQ=255)
                                                                                                            ttCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAAc                                   >  2:1834689/1‑148 (MQ=255)
                                                                                                             tCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                  <  2:1934064/148‑1 (MQ=255)
                                                                                                             tCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                  >  1:2615059/1‑148 (MQ=255)
                                                                                                              ccccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACaa                                 <  1:2578062/148‑1 (MQ=255)
                                                                                                               cccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGACCAAt                                >  2:1239872/1‑148 (MQ=255)
                                                                                                               cccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAAt                                >  2:1240349/1‑148 (MQ=255)
                                                                                                               cccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAAt                                <  1:2467017/148‑1 (MQ=255)
                                                                                                                ccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                               >  2:1609393/1‑148 (MQ=255)
                                                                                                                ccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                               <  2:1767930/148‑1 (MQ=255)
                                                                                                                 cGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGc                              <  1:2292783/148‑1 (MQ=255)
                                                                                                                   aCCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCcg                            >  1:1603774/1‑148 (MQ=255)
                                                                                                                    ccATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAAGTCAGGTTTTACACGGCGTAATACGGGAACAATGCcgc                           >  1:1953712/1‑148 (MQ=255)
                                                                                                                       tGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGa                        >  1:2683116/1‑148 (MQ=255)
                                                                                                                           aGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATAtt                    >  2:2081881/1‑148 (MQ=255)
                                                                                                                            gTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTa                   <  2:404506/148‑1 (MQ=255)
                                                                                                                              tCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTaaa                 <  2:3126662/148‑1 (MQ=255)
                                                                                                                                aggcaggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAAtt               <  1:2126481/148‑1 (MQ=255)
                                                                                                                                   caggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAAttctt            <  1:2407549/148‑1 (MQ=255)
                                                                                                                                   caggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAAttctt            <  1:1900336/148‑1 (MQ=255)
                                                                                                                                     ggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTcc          >  2:1064403/1‑148 (MQ=255)
                                                                                                                                     ggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTcc          >  2:661913/1‑148 (MQ=255)
                                                                                                                                         aTCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTa      >  1:1946408/1‑148 (MQ=255)
                                                                                                                                          tCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTAc     >  1:3244972/1‑148 (MQ=255)
                                                                                                                                             cAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCTAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGa  >  1:2758117/1‑148 (MQ=255)
                                                                                                                                             cAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCTAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGa  >  1:180942/1‑148 (MQ=255)
                                                                                                                                             cAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCTAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGa  >  1:1983424/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGA  >  CP009273/4520556‑4520844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑