Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,545,608 C→A 100% R177I (AGA→ATA)  yjiC ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,545,6080CA97.0% 745.2 / 11.2 237R177I (AGA→ATA) yjiCuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (111/119);  total (113/124)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

TTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTA  >  CP009273/4545463‑4545752
                                                                                                                                                 |                                                                                                                                                
ttCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATAtt                                                                                                                                                <  1:1041014/148‑1 (MQ=255)
ttCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATAtt                                                                                                                                                >  1:1857526/1‑148 (MQ=255)
       ccAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCt                                                                                                                                         <  1:481776/148‑1 (MQ=255)
       ccAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCt                                                                                                                                         <  1:3021591/148‑1 (MQ=255)
        cAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTg                                                                                                                                        >  1:3009307/1‑148 (MQ=255)
          attattGGGTCATTACCCAACTCAATGTTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTgag                                                                                                                                      >  2:1798041/1‑148 (MQ=255)
             attGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAg                                                                                                                                   <  2:3071109/148‑1 (MQ=255)
             attGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAg                                                                                                                                   <  2:2598215/148‑1 (MQ=255)
                  gTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGa                                                                                                                              <  1:2575891/148‑1 (MQ=255)
                    cATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGacg                                                                                                                            >  2:1848002/1‑146 (MQ=255)
                    cATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAg                                                                                                                            <  2:3450741/148‑1 (MQ=255)
                     aTTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAgg                                                                                                                           <  2:1117724/148‑1 (MQ=255)
                     aTTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAgg                                                                                                                           >  2:2789956/1‑148 (MQ=255)
                      ttACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAggg                                                                                                                          <  2:675608/148‑1 (MQ=255)
                        aCCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGtt                                                                                                                        <  2:2812041/148‑1 (MQ=255)
                         cccAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTg                                                                                                                       >  2:876666/1‑148 (MQ=255)
                         cccAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTg                                                                                                                       <  2:3378046/148‑1 (MQ=255)
                            aaCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGaaa                                                                                                                    >  1:1556684/1‑148 (MQ=255)
                             aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAat                                                                                                                   >  2:2885532/1‑148 (MQ=255)
                             aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAat                                                                                                                   <  2:752429/148‑1 (MQ=255)
                             aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTAACCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTCGAAGGGTTGAAat                                                                                                                   >  1:1758710/1‑148 (MQ=255)
                                 aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAatatat                                                                                                               <  2:754069/148‑1 (MQ=255)
                                 aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAatatat                                                                                                               <  2:754477/148‑1 (MQ=255)
                                 aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAatatat                                                                                                               <  2:840285/148‑1 (MQ=255)
                                  atatTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATc                                                                                                              <  1:2895148/148‑1 (MQ=255)
                                  atatTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATc                                                                                                              <  1:193643/148‑1 (MQ=255)
                                    atTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCt                                                                                                            >  1:1613943/1‑148 (MQ=255)
                                      tAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTAt                                                                                                          >  2:1698713/1‑148 (MQ=255)
                                        gCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCg                                                                                                        >  2:385392/1‑148 (MQ=255)
                                         cATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGt                                                                                                       >  1:2253125/1‑148 (MQ=255)
                                           ttAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTgg                                                                                                     <  1:65359/148‑1 (MQ=255)
                                             aaGTAATGCGGCAAGGGCGGATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGtt                                                                                                   >  1:3335843/1‑148 (MQ=255)
                                             aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGtt                                                                                                   >  2:917809/1‑148 (MQ=255)
                                               gTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTcc                                                                                                 >  1:995408/1‑148 (MQ=255)
                                               gTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTcc                                                                                                 >  2:2291404/1‑148 (MQ=255)
                                                    gCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCAt                                                                                            >  1:882901/1‑148 (MQ=255)
                                                        cAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAcc                                                                                        >  1:2778959/1‑148 (MQ=255)
                                                         aaGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                                                                                       <  1:1690620/148‑1 (MQ=255)
                                                           gggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                     <  1:1727660/148‑1 (MQ=255)
                                                           gggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                     >  1:1303814/1‑148 (MQ=255)
                                                           gggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                     >  1:2468089/1‑148 (MQ=255)
                                                            ggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa                                                                                    >  1:2269508/1‑148 (MQ=255)
                                                             gCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGaa                                                                                   >  2:784514/1‑148 (MQ=255)
                                                              cGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAAc                                                                                  >  1:3236392/1‑148 (MQ=255)
                                                                aaTCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACAt                                                                                <  2:1932499/148‑1 (MQ=255)
                                                                 aTCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACAtt                                                                               >  1:1180255/1‑148 (MQ=255)
                                                                  tCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTg                                                                              <  2:504307/148‑1 (MQ=255)
                                                                     ggAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCaa                                                                           >  1:3399425/1‑148 (MQ=255)
                                                                     ggAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCaa                                                                           <  2:1556684/148‑1 (MQ=255)
                                                                        agaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCa                                                                        >  2:973833/1‑148 (MQ=255)
                                                                             ttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGcc                                                                   >  1:2914811/1‑148 (MQ=255)
                                                                             ttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGcc                                                                   >  1:232321/1‑148 (MQ=255)
                                                                                 tACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAAt                                                               <  1:919751/148‑1 (MQ=255)
                                                                                   ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGt                                                             <  1:2792001/148‑1 (MQ=255)
                                                                                     ccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTa                                                           >  1:3296282/1‑148 (MQ=255)
                                                                                      cTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTAt                                                          >  2:444102/1‑148 (MQ=255)
                                                                                        gTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTAttt                                                        >  2:165714/1‑148 (MQ=255)
                                                                                          tACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCa                                                      >  1:3537369/1‑148 (MQ=255)
                                                                                           aCCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCAt                                                     >  2:2020535/1‑148 (MQ=255)
                                                                                              gTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                                                  <  2:2127308/148‑1 (MQ=255)
                                                                                              gTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                                                  <  2:2093086/148‑1 (MQ=255)
                                                                                                cATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACt                                                >  1:1034515/1‑148 (MQ=255)
                                                                                                cATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACt                                                >  2:3558693/1‑148 (MQ=255)
                                                                                                cATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACt                                                >  2:1275570/1‑148 (MQ=255)
                                                                                                  ttATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGt                                              >  1:2540645/1‑148 (MQ=255)
                                                                                                  ttATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGt                                              <  2:53108/148‑1 (MQ=255)
                                                                                                  ttATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGt                                              >  2:2158455/1‑148 (MQ=255)
                                                                                                  ttATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGt                                              <  1:67850/148‑1 (MQ=255)
                                                                                                     tCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGtata                                           <  2:2923743/148‑1 (MQ=255)
                                                                                                      cTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAg                                          <  2:1488404/148‑1 (MQ=255)
                                                                                                       tGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGt                                         <  1:1016483/148‑1 (MQ=255)
                                                                                                           cAATATTTATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGTTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTc                                     <  2:3274977/148‑1 (MQ=255)
                                                                                                           cAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTc                                     >  1:2024908/1‑148 (MQ=255)
                                                                                                             atatAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCaa                                   <  1:2554890/148‑1 (MQ=255)
                                                                                                             atatAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCaa                                   >  1:407129/1‑148 (MQ=255)
                                                                                                                 aGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGACATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAgg                               >  2:1347063/1‑148 (MQ=255)
                                                                                                                  gATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGa                              <  2:1029472/148‑1 (MQ=255)
                                                                                                                  gATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGa                              >  1:1709609/1‑148 (MQ=255)
                                                                                                                  gATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGa                              <  2:1613943/148‑1 (MQ=255)
                                                                                                                  gATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGa                              >  1:427338/1‑148 (MQ=255)
                                                                                                                    tatCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATc                            >  2:395396/1‑148 (MQ=255)
                                                                                                                    tatCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATc                            >  2:894439/1‑148 (MQ=255)
                                                                                                                       cAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGaa                         <  2:1908417/148‑1 (MQ=255)
                                                                                                                       cAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGaa                         >  1:3465199/1‑148 (MQ=255)
                                                                                                                          tCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAgg                      <  2:1897273/148‑1 (MQ=255)
                                                                                                                          tCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAgg                      >  2:298398/1‑148 (MQ=255)
                                                                                                                           ccTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAggg                     >  1:3355701/1‑148 (MQ=255)
                                                                                                                             ttGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGct                   <  1:833631/148‑1 (MQ=255)
                                                                                                                              tGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGctc                  >  1:529607/1‑148 (MQ=255)
                                                                                                                               ggCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGctct                 >  1:1426819/1‑148 (MQ=255)
                                                                                                                               ggCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGctct                 <  1:268280/148‑1 (MQ=255)
                                                                                                                                   gctCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCat             >  2:2843508/1‑148 (MQ=255)
                                                                                                                                      cATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaa          <  2:3185613/148‑1 (MQ=255)
                                                                                                                                      cATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaa          <  1:1798041/148‑1 (MQ=255)
                                                                                                                                      cATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGACATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaa          <  1:2375699/148‑1 (MQ=255)
                                                                                                                                        ttGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaata        <  2:2515188/148‑1 (MQ=255)
                                                                                                                                         tGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaataa       <  1:3118348/148‑1 (MQ=255)
                                                                                                                                            tCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAAtt    <  2:2373199/148‑1 (MQ=255)
                                                                                                                                              cATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTa  >  1:2169737/1‑148 (MQ=255)
                                                                                                                                              cATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTa  <  2:114059/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTA  >  CP009273/4545463‑4545752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑