Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,547,818 C→A 100% D97Y (GAT→TAT)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,547,8180CA97.6% 699.0 / 6.0 223D97Y (GAT→TAT) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base A (110/107);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

TTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAG  >  CP009273/4547672‑4547962
                                                                                                                                                  |                                                                                                                                                
ttCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCg                                                                                                                                                 <  2:2946566/148‑1 (MQ=255)
 tCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGc                                                                                                                                                >  2:2587921/1‑148 (MQ=255)
   aCGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTg                                                                                                                                              <  2:2865401/148‑1 (MQ=255)
    cGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTgc                                                                                                                                             <  2:566068/148‑1 (MQ=255)
        gTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTgccgcc                                                                                                                                         >  1:816319/1‑148 (MQ=255)
           tATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACg                                                                                                                                      <  2:2092138/148‑1 (MQ=255)
              cGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAg                                                                                                                                   >  2:1603110/1‑148 (MQ=255)
              cGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAg                                                                                                                                   >  1:965950/1‑148 (MQ=255)
              cGACCGCTTAATCGACATCAAGAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACTGTAACAGCCCGAGTGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAg                                                                                                                                   <  2:3022022/148‑1 (MQ=255)
                aCCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGct                                                                                                                                 <  1:1031955/148‑1 (MQ=255)
                aCCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGct                                                                                                                                 >  2:154287/1‑148 (MQ=255)
                  cGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGctct                                                                                                                               >  2:2167582/1‑148 (MQ=255)
                    cTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGc                                                                                                                             <  1:325239/148‑1 (MQ=255)
                        aTCGACATCAAGAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGa                                                                                                                         <  1:3321814/148‑1 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGANAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGc                                                                                                                     <  1:1809470/148‑1 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGACAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGc                                                                                                                     >  2:2295246/1‑148 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGc                                                                                                                     >  1:1554639/1‑148 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGc                                                                                                                     >  2:1930182/1‑148 (MQ=255)
                             cATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGct                                                                                                                    >  2:3413402/1‑148 (MQ=255)
                              aTCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctc                                                                                                                   >  1:2505250/1‑148 (MQ=255)
                              aTCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctc                                                                                                                   >  1:2505317/1‑148 (MQ=255)
                               tCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctct                                                                                                                  <  2:2349960/148‑1 (MQ=255)
                               tCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctct                                                                                                                  >  1:1088441/1‑148 (MQ=255)
                                cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctc                                                                                                                 <  1:2398785/148‑1 (MQ=255)
                                cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctc                                                                                                                 >  2:2065991/1‑148 (MQ=255)
                                  aTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTACTAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTa                                                                                                               >  2:2689367/1‑148 (MQ=255)
                                    aGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAAc                                                                                                             >  2:1062109/1‑148 (MQ=255)
                                    aGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAAc                                                                                                             >  1:3455827/1‑148 (MQ=255)
                                      cTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTg                                                                                                           <  1:24670/148‑1 (MQ=255)
                                        tCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGtt                                                                                                         >  1:3112216/1‑148 (MQ=255)
                                         cAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTg                                                                                                        <  2:383684/148‑1 (MQ=255)
                                          aaTTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGc                                                                                                       >  2:2963450/1‑148 (MQ=255)
                                           aTTGCCCAGGTAAAGAGCGGCGGCGTCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCa                                                                                                      >  2:3174508/1‑148 (MQ=255)
                                           aTTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCa                                                                                                      >  2:1789182/1‑148 (MQ=255)
                                            ttGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCaa                                                                                                     >  2:2775207/1‑148 (MQ=255)
                                            ttGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCaa                                                                                                     >  2:2775307/1‑148 (MQ=255)
                                            ttGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCaa                                                                                                     >  2:2373145/1‑148 (MQ=255)
                                             tGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAAc                                                                                                    >  1:892973/1‑148 (MQ=255)
                                               cccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAg                                                                                                  <  1:3325751/148‑1 (MQ=255)
                                                     tAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCg                                                                                            <  1:158723/148‑1 (MQ=255)
                                                     tAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCg                                                                                            >  2:210752/1‑148 (MQ=255)
                                                      aaaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCgg                                                                                           >  1:2980870/1‑148 (MQ=255)
                                                       aaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                          >  1:2219303/1‑148 (MQ=255)
                                                       aaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                          >  1:1420927/1‑148 (MQ=255)
                                                       aaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                          >  1:3192609/1‑148 (MQ=255)
                                                            cggcggCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGAGTGCTCTCGAACTGTTGCAACAGATGGCGGATCTGc                                                                                     >  1:2604835/1‑148 (MQ=255)
                                                                 gcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATg                                                                                >  1:266133/1‑148 (MQ=255)
                                                                 gcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATg                                                                                <  2:816319/148‑1 (MQ=255)
                                                                  cATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATgg                                                                               >  1:1505642/1‑148 (MQ=255)
                                                                   aTCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGa                                                                              <  2:1572306/148‑1 (MQ=255)
                                                                     cTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGa                                                                                                 <  2:2494513/127‑1 (MQ=255)
                                                                     cTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAg                                                                            <  2:2270827/148‑1 (MQ=255)
                                                                     cTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAg                                                                            <  2:1323146/148‑1 (MQ=255)
                                                                      tGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGa                                                                           <  1:2786550/148‑1 (MQ=255)
                                                                        gCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtt                                                                         >  2:823920/1‑148 (MQ=255)
                                                                          tGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtttt                                                                       <  1:1322910/148‑1 (MQ=255)
                                                                          tGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtttt                                                                       <  2:117724/148‑1 (MQ=255)
                                                                           gATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTc                                                                      <  1:3156488/148‑1 (MQ=255)
                                                                            aTAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTcc                                                                     <  2:3364939/148‑1 (MQ=255)
                                                                               aTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCtt                                                                  <  1:2735652/148‑1 (MQ=255)
                                                                                tGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                 <  2:1813494/148‑1 (MQ=255)
                                                                                 ggACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGt                                                                >  2:2718381/1‑148 (MQ=255)
                                                                                 ggACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGt                                                                <  2:352844/148‑1 (MQ=255)
                                                                                  gACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGtt                                                               >  1:492905/1‑148 (MQ=255)
                                                                                         aCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                        <  1:190685/148‑1 (MQ=255)
                                                                                         aCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                        >  1:3179288/1‑148 (MQ=255)
                                                                                            gCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTg                                                     <  1:3465564/148‑1 (MQ=255)
                                                                                            gCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTg                                                     <  1:1750053/148‑1 (MQ=255)
                                                                                            gCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTg                                                     <  1:2303189/148‑1 (MQ=255)
                                                                                               cGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAg                                                  <  2:3127138/148‑1 (MQ=255)
                                                                                               cGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGATCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAg                                                  <  2:3498089/148‑1 (MQ=255)
                                                                                                 aGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcg                                                >  2:1739728/1‑148 (MQ=255)
                                                                                                 aGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcg                                                >  2:3502529/1‑148 (MQ=255)
                                                                                                  gggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcgg                                               <  1:1403435/148‑1 (MQ=255)
                                                                                                  gggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcgg                                               >  2:1574824/1‑148 (MQ=255)
                                                                                                  gggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcgg                                               <  1:3138774/148‑1 (MQ=255)
                                                                                                   ggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcggc                                              <  1:1293409/148‑1 (MQ=255)
                                                                                                    gAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcggcg                                             >  2:169010/1‑148 (MQ=255)
                                                                                                     aaaGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGt                                            >  2:544257/1‑148 (MQ=255)
                                                                                                        gagagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTAc                                         <  2:1239136/148‑1 (MQ=255)
                                                                                                         agagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTAcc                                        <  2:1554646/148‑1 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTg                                      >  2:2133157/1‑148 (MQ=255)
                                                                                                                   gCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTaa                              >  2:1217162/1‑148 (MQ=255)
                                                                                                                      gCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACa                           <  2:2804978/148‑1 (MQ=255)
                                                                                                                      gCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACa                           <  2:3179288/148‑1 (MQ=255)
                                                                                                                      gCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACa                           >  1:1881609/1‑148 (MQ=255)
                                                                                                                      gCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACa                           <  2:2806087/148‑1 (MQ=255)
                                                                                                                          tCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTc                       >  2:1005456/1‑148 (MQ=255)
                                                                                                                          tCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTc                       >  2:669819/1‑148 (MQ=255)
                                                                                                                          tCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTc                       >  2:670801/1‑148 (MQ=255)
                                                                                                                           cTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                      >  2:1960387/1‑148 (MQ=255)
                                                                                                                           cTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                      <  1:3025987/148‑1 (MQ=255)
                                                                                                                            ttGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCaa                     <  2:2980870/148‑1 (MQ=255)
                                                                                                                               aTTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTCACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGAAGCGGCGTTACCTGGCGGTTAAACACTTCAacac                  >  2:1866801/1‑148 (MQ=255)
                                                                                                                               aTTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAacac                  >  2:1866867/1‑148 (MQ=255)
                                                                                                                                tttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACAcc                 >  2:83419/1‑148 (MQ=255)
                                                                                                                                  tttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCaa               >  1:1321639/1‑148 (MQ=255)
                                                                                                                                        tGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCt         >  1:2841238/1‑148 (MQ=255)
                                                                                                                                               aaTAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAg  >  2:1454863/1‑148 (MQ=255)
                                                                                                                                               aaTAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAg  >  2:771870/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAG  >  CP009273/4547672‑4547962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑