Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,548,084 A→T 59.5% L8* (TTG→TAG)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,548,0840AT59.5% 148.8 / 265.6 207L8* (TTG→TAG) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base A (36/48);  new base T (62/61);  total (98/109)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 213 total aligned reads displayed.

AATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGC  >  CP009273/4547937‑4548229
                                                                                                                                                   |                                                                                                                                                 
aaTTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCa                                                                                                                                                   >  2:2339422/1‑148 (MQ=255)
aaTTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCa                                                                                                                                                   >  2:546709/1‑148 (MQ=255)
     aaCACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATc                                                                                                                                              >  1:281706/1‑148 (MQ=255)
      acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCg                                                                                                                                             >  2:2587480/1‑148 (MQ=255)
       cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGc                                                                                                                                            >  1:562093/1‑148 (MQ=255)
        acCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGca                                                                                                                                           <  1:2934559/148‑1 (MQ=255)
           aaTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcacca                                                                                                                                        >  2:838311/1‑148 (MQ=255)
            aTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcaccac                                                                                                                                       <  2:3455842/148‑1 (MQ=255)
             tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcaccacg                                                                                                                                      <  1:823913/148‑2 (MQ=255)
             tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACcaca                                                                                                                                      <  1:2677682/148‑1 (MQ=255)
               gCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGt                                                                                                                                    <  1:1574824/148‑1 (MQ=255)
                ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTc                                                                                                                                   >  1:1753532/1‑148 (MQ=255)
                ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTc                                                                                                                                   >  1:1084589/1‑148 (MQ=255)
                 cTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCa                                                                                                                                  <  1:320799/148‑1 (MQ=255)
                  tGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCAt                                                                                                                                 >  2:212368/1‑148 (MQ=255)
                   gTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATc                                                                                                                                >  2:3240078/1‑148 (MQ=255)
                     tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCa                                                                                                                              <  1:1005456/148‑1 (MQ=255)
                     tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                                                                                              <  2:3095874/148‑1 (MQ=255)
                     tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                                                                                              <  2:222731/148‑1 (MQ=255)
                       cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCAtt                                                                                                                            <  2:2841238/148‑1 (MQ=255)
                       cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCAtt                                                                                                                            <  1:638584/148‑1 (MQ=255)
                       cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCAtt                                                                                                                            <  1:669920/148‑1 (MQ=255)
                       cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCAtt                                                                                                                            <  1:2431858/148‑1 (MQ=255)
                       cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAtt                                                                                                                            >  2:278149/1‑148 (MQ=255)
                         gcgcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTAc                                                                                                                          >  1:787544/1‑148 (MQ=255)
                         gcgcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTAc                                                                                                                          >  2:2088638/1‑148 (MQ=255)
                         gcgcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTAc                                                                                                                          >  2:3058078/1‑148 (MQ=255)
                           gcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGt                                                                                                                        <  1:3369328/148‑1 (MQ=255)
                           gcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGt                                                                                                                        <  1:194043/148‑1 (MQ=255)
                            cACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGtt                                                                                                                       <  1:2255816/148‑1 (MQ=255)
                            cACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGtt                                                                                                                       <  1:2718381/148‑1 (MQ=255)
                                     ggcggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGt                                                                                                              >  2:3407699/1‑148 (MQ=255)
                                     ggcggcTGAAAGCCGGTTGCGAGACCTTGCGACTTACTGCCGCCTGGGAAAAATTTCGGCATTTTTACAGGGTCAGAAAATCATAAATCCAAATGGTTTCAATATTATTATAAATCGCACCAAACGCATCCATTAAGTTTTACTCTGt                                                                                                              >  1:2419948/1‑148 (MQ=255)
                                        ggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAg                                                                                                           <  1:1715754/148‑1 (MQ=255)
                                             aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                      >  1:2461904/1‑148 (MQ=255)
                                                gCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCtt                                                                                                   <  2:1481588/148‑1 (MQ=255)
                                                gCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCtt                                                                                                   <  2:1803852/148‑1 (MQ=255)
                                                 ccGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTg                                                                                                  <  1:2506469/148‑1 (MQ=255)
                                                     ttGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                                                                                              >  2:2429735/1‑148 (MQ=255)
                                                       gCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCa                                                                                            <  2:3337732/148‑1 (MQ=255)
                                                         gagaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAtt                                                                                          <  1:1490161/148‑1 (MQ=255)
                                                            aCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                                                                       >  2:1224902/1‑148 (MQ=255)
                                                               ttGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGt                                                                                    <  2:1724047/148‑1 (MQ=255)
                                                                tGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTc                                                                                   <  1:3230388/148‑1 (MQ=255)
                                                                 gCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTcc                                                                                  <  2:1437077/148‑1 (MQ=255)
                                                                    actgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCAtt                                                                               <  1:1793914/148‑1 (MQ=255)
                                                                       gactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCgg                                                                            >  2:405603/1‑148 (MQ=255)
                                                                        actgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCggg                                                                           >  2:643733/1‑148 (MQ=255)
                                                                           gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcgg                                                                        <  2:3131984/148‑1 (MQ=255)
                                                                           gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcgg                                                                        >  1:2093022/1‑148 (MQ=255)
                                                                                  gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                 >  1:867356/1‑148 (MQ=255)
                                                                                   ggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTTCGGGCATTCTACGTCCATTCGGGCGGCTGACAAc                                                                <  2:1778201/148‑1 (MQ=255)
                                                                                   ggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAc                                                                >  2:2524031/1‑148 (MQ=255)
                                                                                    gAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAcc                                                               >  1:830746/1‑148 (MQ=255)
                                                                                     aaaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCg                                                              <  1:1164444/148‑1 (MQ=255)
                                                                                     aaaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCg                                                              >  1:2171601/1‑148 (MQ=255)
                                                                                      aaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGt                                                             <  2:1879475/148‑1 (MQ=255)
                                                                                      aaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGt                                                             <  1:1388988/148‑1 (MQ=255)
                                                                                        aaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGttt                                                           <  2:1759297/148‑1 (MQ=255)
                                                                                        aaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGttt                                                           <  2:1760691/148‑1 (MQ=255)
                                                                                           tGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTAGTCTGTCAGCCGCCCTTGCGGGCATGCTACGTCCATTCGGGCGGCTGACAACCGTTTAAg                                                        >  2:2884884/1‑148 (MQ=255)
                                                                                             cGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGcc                                                      <  1:298180/148‑1 (MQ=255)
                                                                                              ggCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCg                                                     <  1:3502529/148‑1 (MQ=255)
                                                                                              ggCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCg                                                     <  1:1739728/148‑1 (MQ=255)
                                                                                                cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                   <  1:2255667/148‑1 (MQ=255)
                                                                                                 aTTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                  <  2:3554406/148‑1 (MQ=255)
                                                                                                 aTTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                  <  1:434551/148‑1 (MQ=255)
                                                                                                   ttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCa                                                <  2:787549/148‑1 (MQ=255)
                                                                                                    tttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCaa                                               >  2:1400152/1‑148 (MQ=255)
                                                                                                       ccAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACg                                            >  1:1065114/1‑148 (MQ=255)
                                                                                                         aGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGtt                                          >  2:757442/1‑148 (MQ=255)
                                                                                                         aGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGtt                                          >  2:1060176/1‑148 (MQ=255)
                                                                                                         aGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGtt                                          <  2:3395765/148‑1 (MQ=255)
                                                                                                          gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                         <  1:2106811/148‑1 (MQ=255)
                                                                                                          gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                         <  1:82733/148‑1 (MQ=255)
                                                                                                              cAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCttt                                     >  2:3477688/1‑148 (MQ=255)
                                                                                                              cAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCttt                                     >  2:1296467/1‑148 (MQ=255)
                                                                                                              cAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCttt                                     <  1:799622/148‑1 (MQ=255)
                                                                                                               aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTc                                    <  1:266199/148‑1 (MQ=255)
                                                                                                                gAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACNCTGTCAGCCGCCCTTGCGNGCATTCTACGTCCATTCGGGCGGCTGACAANCGTTTAAGCCGTTTCAAACGTTCCTTTCa                                   <  2:2871163/148‑1 (MQ=255)
                                                                                                                   aaTCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGc                                <  1:1882058/148‑1 (MQ=255)
                                                                                                                     tCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAg                              >  1:113952/1‑148 (MQ=255)
                                                                                                                       aTAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGc                            >  2:2682339/1‑148 (MQ=255)
                                                                                                                        tAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCt                           >  2:1179755/1‑148 (MQ=255)
                                                                                                                        tAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCt                           <  2:2461904/148‑1 (MQ=255)
                                                                                                                           aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTg                        >  1:300634/1‑148 (MQ=255)
                                                                                                                                tttGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                   >  2:2007113/1‑148 (MQ=255)
                                                                                                                                   ggTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCttt                <  2:827688/148‑1 (MQ=255)
                                                                                                                                    gTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTg               <  1:1982558/148‑1 (MQ=255)
                                                                                                                                      ttCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGAc             <  1:2879065/148‑1 (MQ=255)
                                                                                                                                      ttCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGAc             >  1:3279737/1‑148 (MQ=255)
                                                                                                                                        cAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCa           >  1:1374540/1‑148 (MQ=255)
                                                                                                                                         aaTATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCAt          <  1:2013648/148‑1 (MQ=255)
                                                                                                                                          atatTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATc         >  1:1106171/1‑148 (MQ=255)
                                                                                                                                           tattatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa        <  2:2945915/148‑1 (MQ=255)
                                                                                                                                              tatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt     >  1:1967339/1‑148 (MQ=255)
                                                                                                                                              tatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt     >  2:1270701/1‑148 (MQ=255)
                                                                                                                                              tatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCAGTCTTTGACCATCAGtt     >  1:3045582/1‑148 (MQ=255)
                                                                                                                                              tatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt     >  1:671068/1‑148 (MQ=255)
                                                                                                                                                 gCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGc  >  2:709539/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGC  >  CP009273/4547937‑4548229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑