Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,555,156 G→T 100% A171E (GCG→GAG)  yjiM ← putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,555,1560GT99.0% 617.6 / ‑1.9 190A171E (GCG→GAG) yjiMputative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (94/94);  total (95/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

CAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAAC  >  CP009273/4555011‑4555298
                                                                                                                                                 |                                                                                                                                              
cAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCa                                                                                                                                              <  2:1414182/148‑1 (MQ=255)
   ttCATTGATCAACGCCTCTTTATCGGACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATg                                                                                                                                           <  1:1406007/148‑1 (MQ=255)
   ttCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGTGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATg                                                                                                                                           >  2:1084178/1‑148 (MQ=255)
    tCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATgg                                                                                                                                          <  2:92381/148‑1 (MQ=255)
      aTTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCa                                                                                                                                        <  1:1075155/148‑1 (MQ=255)
         gATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATcg                                                                                                                                     <  2:2049086/148‑1 (MQ=255)
          aTCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATcgc                                                                                                                                    >  1:1241902/1‑148 (MQ=255)
          aTCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATcgc                                                                                                                                    >  1:1746750/1‑148 (MQ=255)
            cAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCgcgc                                                                                                                                  <  2:281358/148‑1 (MQ=255)
            cAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCgcgc                                                                                                                                  >  1:2082116/1‑148 (MQ=255)
                  ctctTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCa                                                                                                                            <  2:3461650/148‑1 (MQ=255)
                    ctTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATc                                                                                                                          <  2:3415101/148‑1 (MQ=255)
                      ttATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCtt                                                                                                                        >  1:1903658/1‑148 (MQ=255)
                      ttATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCtt                                                                                                                        >  1:97708/1‑148 (MQ=255)
                        aTCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCg                                                                                                                      >  2:1110558/1‑148 (MQ=255)
                        aTCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCg                                                                                                                      >  1:3420240/1‑148 (MQ=255)
                             aCCGGTAGGTGGCGGCGTAAACCCCTTTCTGTTTGTCGCTGGCTCTAAGCGACGGAGGATTTTAGTGCCCAATATTATACAAATTAGCCAGTGCGCGACGTTCGCTGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAAt                                                                                                                 <  2:739089/148‑1 (MQ=255)
                             aCCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAAt                                                                                                                 <  2:739123/148‑1 (MQ=255)
                              ccGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAAtc                                                                                                                >  2:1204517/1‑148 (MQ=255)
                              ccGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAAtc                                                                                                                <  1:2641502/148‑1 (MQ=255)
                                 gAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCg                                                                                                             >  1:3186609/1‑148 (MQ=255)
                                       tGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCa                                                                                                       <  1:1064438/148‑1 (MQ=255)
                                       tGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCa                                                                                                       <  1:1064208/148‑1 (MQ=255)
                                         cgcCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCaaa                                                                                                     <  2:1613785/148‑1 (MQ=255)
                                          gcCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCaaaa                                                                                                    <  1:2149005/148‑1 (MQ=255)
                                           ccGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAAc                                                                                                   >  2:1837627/1‑148 (MQ=255)
                                            cGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACg                                                                                                  >  2:1447092/1‑148 (MQ=255)
                                             gTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGt                                                                                                 <  2:1697662/148‑1 (MQ=255)
                                              tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                <  1:1818519/148‑1 (MQ=255)
                                              tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                >  2:2169603/1‑148 (MQ=255)
                                              tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                <  1:311518/148‑1 (MQ=255)
                                                  ccACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttcttc                                                                                            <  2:1767094/148‑1 (MQ=255)
                                                     cTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTAc                                                                                         <  2:2349559/148‑1 (MQ=255)
                                                      tttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTAcc                                                                                        >  2:3373133/1‑148 (MQ=255)
                                                       ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCg                                                                                       <  1:427095/148‑1 (MQ=255)
                                                       ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCg                                                                                       <  2:799999/148‑1 (MQ=255)
                                                       ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCg                                                                                       <  1:2152250/148‑1 (MQ=255)
                                                        tCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGt                                                                                      >  2:2392490/1‑148 (MQ=255)
                                                         cAGAAGGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGtt                                                                                     <  2:1974658/148‑1 (MQ=255)
                                                            aaTGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttttt                                                                                  >  2:2373042/1‑148 (MQ=255)
                                                            aaTGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACAGttttt                                                                                  >  2:990604/1‑148 (MQ=255)
                                                                tCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCa                                                                              <  1:3354031/148‑1 (MQ=255)
                                                                 cGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCaa                                                                             <  2:3433547/148‑1 (MQ=255)
                                                                   cTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAgc                                                                           >  2:2214840/1‑148 (MQ=255)
                                                                   cTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAgc                                                                           >  2:2055690/1‑148 (MQ=255)
                                                                        gCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCgcagc                                                                      <  2:3537410/148‑1 (MQ=255)
                                                                        gCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCgcagc                                                                      <  1:252274/148‑1 (MQ=255)
                                                                         cTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCgcagca                                                                     >  1:44055/1‑148 (MQ=255)
                                                                          tAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAt                                                                    <  1:994774/148‑1 (MQ=255)
                                                                           acgcgcCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   <  2:2995161/146‑1 (MQ=255)
                                                                           aaGCTCCGGATGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCTGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   <  2:1419815/148‑1 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATTGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   >  1:2901294/1‑148 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   <  2:2995192/148‑1 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   >  2:59757/1‑148 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                   >  1:1526723/1‑148 (MQ=255)
                                                                            aGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtct                                                                  >  1:1124235/1‑148 (MQ=255)
                                                                                ccGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGc                                                              <  2:368550/148‑1 (MQ=255)
                                                                                 cGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCt                                                             <  2:301843/148‑1 (MQ=255)
                                                                                      gaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCa                                                        >  1:3055607/1‑148 (MQ=255)
                                                                                      gaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCa                                                        >  1:1363221/1‑148 (MQ=255)
                                                                                       aTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCAt                                                       >  1:991731/1‑148 (MQ=255)
                                                                                        tttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATa                                                      <  2:1484581/148‑1 (MQ=255)
                                                                                         ttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATaa                                                     <  1:2726862/148‑1 (MQ=255)
                                                                                         ttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATaa                                                     >  2:2430688/1‑148 (MQ=255)
                                                                                            aCTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAactc                                                  >  2:129216/1‑146 (MQ=255)
                                                                                             cTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAacgca                                                 >  1:44937/1‑148 (MQ=255)
                                                                                             cTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAacgca                                                 >  1:557541/1‑148 (MQ=255)
                                                                                               gCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAacgcacg                                               >  2:2799489/1‑148 (MQ=255)
                                                                                                  cAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGa                                            >  2:2263632/1‑148 (MQ=255)
                                                                                                    aGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAgg                                          >  2:2532391/1‑148 (MQ=255)
                                                                                                     gatgatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGc                                         >  1:1563193/1‑148 (MQ=255)
                                                                                                      atgatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCa                                        <  2:2014619/148‑1 (MQ=255)
                                                                                                        gatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATc                                      >  1:985757/1‑148 (MQ=255)
                                                                                                        gatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATc                                      >  1:927206/1‑148 (MQ=255)
                                                                                                         atAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCg                                     <  1:880255/148‑1 (MQ=255)
                                                                                                         atAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCg                                     >  2:1384258/1‑148 (MQ=255)
                                                                                                            aaaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTcc                                  <  1:2581687/148‑1 (MQ=255)
                                                                                                             aaaTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCt                                 >  1:2456856/1‑148 (MQ=255)
                                                                                                              aaTTAGCCAGTGCGTGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCtt                                <  2:3042446/148‑1 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                              >  2:1912389/1‑148 (MQ=255)
                                                                                                                   gCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGc                           >  2:3142131/1‑148 (MQ=255)
                                                                                                                    ccAGTGCGCGACGTTCGCGGTTTTTTAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCt                          >  2:2237467/1‑148 (MQ=255)
                                                                                                                       gTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGtt                       >  2:538489/1‑148 (MQ=255)
                                                                                                                        tGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTg                      <  1:647126/148‑1 (MQ=255)
                                                                                                                             gACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCaa                 <  2:1241902/148‑1 (MQ=255)
                                                                                                                             gACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCaa                 <  2:437285/148‑1 (MQ=255)
                                                                                                                             gACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCaa                 >  1:3101742/1‑148 (MQ=255)
                                                                                                                              aCGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAAt                >  2:1499036/1‑148 (MQ=255)
                                                                                                                               cGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAAtt               <  2:3341581/148‑1 (MQ=255)
                                                                                                                                 ttCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGc             <  2:3094864/148‑1 (MQ=255)
                                                                                                                                      ggTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcac        <  1:3562350/148‑1 (MQ=255)
                                                                                                                                      ggTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcac        <  1:2961761/148‑1 (MQ=255)
                                                                                                                                      ggTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcac        <  1:3562246/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca       <  1:488682/148‑1 (MQ=255)
                                                                                                                                        tttttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACAt      >  2:3131458/1‑148 (MQ=255)
                                                                                                                                        tttttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACAt      >  2:3049021/1‑148 (MQ=255)
                                                                                                                                        tttttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACAt      <  2:1558495/148‑1 (MQ=255)
                                                                                                                                         ttttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATg     >  1:1121842/1‑148 (MQ=255)
                                                                                                                                         ttttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATg     <  2:894287/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGa    <  1:204451/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAAC  >  CP009273/4555011‑4555298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑