Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,571,233 G→A 100% S16F (TCT→TTT)  hsdS ← specificity determinant for hsdM and hsdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,571,2330GA98.5% 650.4 / ‑0.4 199S16F (TCT→TTT) hsdSspecificity determinant for hsdM and hsdR
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (110/86);  total (112/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

TTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAG  >  CP009273/4571088‑4571377
                                                                                                                                                 |                                                                                                                                                
tttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                >  1:1078576/1‑148 (MQ=255)
tttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                <  2:1652138/148‑1 (MQ=255)
 ttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATa                                                                                                                                               <  1:91792/148‑1 (MQ=255)
    ggAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTg                                                                                                                                            <  1:2298390/148‑1 (MQ=255)
     gAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTgg                                                                                                                                           <  1:847826/148‑1 (MQ=255)
     gAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTgg                                                                                                                                           >  1:2403547/1‑148 (MQ=255)
        cAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGc                                                                                                                                        >  1:206951/1‑148 (MQ=255)
              ccAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAac                                                                                                                                  <  2:2151149/148‑1 (MQ=255)
              ccAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAac                                                                                                                                  >  1:1915632/1‑148 (MQ=255)
               cAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacc                                                                                                                                 <  2:2781145/148‑1 (MQ=255)
                  gTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccac                                                                                                                              >  1:3171348/1‑148 (MQ=255)
                  gTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccac                                                                                                                              >  2:3118389/1‑148 (MQ=255)
                   tCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccacc                                                                                                                             >  2:1269564/1‑148 (MQ=255)
                   tCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccacc                                                                                                                             >  2:2449598/1‑148 (MQ=255)
                      gtagtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCt                                                                                                                          <  2:862654/148‑1 (MQ=255)
                      gtagtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCt                                                                                                                          <  2:1065530/148‑1 (MQ=255)
                        agtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTcc                                                                                                                        >  2:3158112/1‑148 (MQ=255)
                         gtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCg                                                                                                                       <  2:191039/148‑1 (MQ=255)
                          taTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCgg                                                                                                                      >  2:1759642/1‑148 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   <  2:926398/148‑1 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   <  1:1625745/148‑1 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   >  2:2803195/1‑148 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGAAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   <  1:3294006/148‑1 (MQ=255)
                                 cTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTc                                                                                                               <  2:461962/148‑1 (MQ=255)
                                  ttGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTcc                                                                                                              <  2:2489658/148‑1 (MQ=255)
                                         tCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                       >  2:3428801/1‑148 (MQ=255)
                                           tGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCa                                                                                                     <  1:3118389/148‑1 (MQ=255)
                                              atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                  <  2:1789345/148‑1 (MQ=255)
                                              atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                  <  1:2777704/148‑1 (MQ=255)
                                              atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                  <  1:290289/148‑1 (MQ=255)
                                               tatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                 >  1:2586505/1‑148 (MQ=255)
                                                  tGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCTTc                                                                                              >  1:2362419/1‑148 (MQ=255)
                                                  tGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTc                                                                                              <  1:495144/148‑1 (MQ=255)
                                                    ttCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAc                                                                                            >  2:1088943/1‑148 (MQ=255)
                                                     tCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAcc                                                                                           >  2:2798898/1‑148 (MQ=255)
                                                      cGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAccc                                                                                          >  1:2540669/1‑148 (MQ=255)
                                                      cGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAccc                                                                                          >  1:2539824/1‑148 (MQ=255)
                                                      cGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAAATTCCCCGCACTCATTCCTTCAccc                                                                                          >  1:2275608/1‑148 (MQ=255)
                                                        cACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCCCCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCcca                                                                                        >  2:1499111/1‑148 (MQ=255)
                                                        cACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCcca                                                                                        >  2:518102/1‑148 (MQ=255)
                                                        cACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCcca                                                                                        >  2:3270019/1‑148 (MQ=255)
                                                         aCGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCccac                                                                                       <  2:3383419/148‑1 (MQ=255)
                                                           ggATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCccacca                                                                                     <  1:538275/148‑1 (MQ=255)
                                                            gATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCaa                                                                                    <  1:948902/148‑1 (MQ=255)
                                                               aaGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACg                                                                                 >  2:668730/1‑148 (MQ=255)
                                                                agagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGc                                                                                >  1:3381828/1‑148 (MQ=255)
                                                                 gagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGct                                                                               >  1:1390931/1‑148 (MQ=255)
                                                                    gCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCACTCATTCCTTCACCCCACCAAACGcttct                                                                            >  2:404926/1‑148 (MQ=255)
                                                                     cAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                           <  1:1399342/148‑1 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         >  2:2967058/1‑148 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         <  2:95800/148‑1 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         <  1:1079978/148‑1 (MQ=255)
                                                                          aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACCCTTCTTCCAGc                                                                      >  2:3151209/1‑148 (MQ=255)
                                                                          aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCAGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGc                                                                      >  2:161773/1‑148 (MQ=255)
                                                                           atcatcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCa                                                                     <  2:675667/148‑1 (MQ=255)
                                                                            tcatcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCaa                                                                    <  1:139840/148‑1 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   >  1:1659177/1‑148 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   >  2:2717233/1‑148 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   >  2:2586631/1‑148 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAATCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   >  2:2584794/1‑148 (MQ=255)
                                                                               tcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTg                                                                 >  2:2308387/1‑148 (MQ=255)
                                                                               tcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTg                                                                 >  2:2422470/1‑148 (MQ=255)
                                                                                   ttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCAATCATTCCTTCACCCCACCAAACCCTTCATCCAGCAAATGACGc                                                             >  1:1702336/1‑148 (MQ=255)
                                                                                        tAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaa                                                        >  2:2407860/1‑148 (MQ=255)
                                                                                        tAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTATTCCACCAACTGACGCTGcca                                                        >  1:621283/1‑146 (MQ=255)
                                                                                         aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaaa                                                       <  2:2007829/148‑1 (MQ=255)
                                                                                         aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATATCCCCGCACTCATTCCTTCAACCCACCAAACGCATCTTCCAGCAACTGACGCTGCaaa                                                       >  2:588249/1‑148 (MQ=255)
                                                                                          aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTGCATCCCCCCACCAAAAGCTTCTTCCAGCAACTGACGCTGCAAAt                                                      >  1:3182215/1‑148 (MQ=255)
                                                                                          aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAAt                                                      >  1:3181574/1‑148 (MQ=255)
                                                                                           tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATc                                                     <  1:2453433/148‑1 (MQ=255)
                                                                                              aTTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGc                                                  >  1:2729234/1‑148 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCCAAAGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                 >  1:3318593/1‑148 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                 <  2:330499/148‑1 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCGTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                 >  1:350366/1‑148 (MQ=255)
                                                                                                tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCCTTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCt                                                >  2:22453/1‑148 (MQ=255)
                                                                                                tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCCAATCGGCCt                                                >  2:23398/1‑148 (MQ=255)
                                                                                                tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACACCTCCGGCAATTTCACCGCAATCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCt                                                >  1:721992/1‑148 (MQ=255)
                                                                                                  ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                              <  2:2524666/148‑1 (MQ=255)
                                                                                                  ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                              <  1:1498179/148‑1 (MQ=255)
                                                                                                   cTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAt                                             <  1:816092/148‑1 (MQ=255)
                                                                                                     gCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcg                                           >  2:1559122/1‑148 (MQ=255)
                                                                                                     gCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcg                                           <  1:433310/148‑1 (MQ=255)
                                                                                                         ttttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCCTTCCTTCACCCCACCAAACGCTTCTTCCAGCCACTGACGCGGGACATCGGCCTCGtcgctcg                                       >  2:3540786/1‑148 (MQ=255)
                                                                                                           ttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcc                                     <  1:788416/148‑1 (MQ=255)
                                                                                                           ttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCAACCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGAAAATGACGCAGCAAAAACGCATCATCGCTCGcc                                     >  2:3475345/1‑148 (MQ=255)
                                                                                                              tataCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGccccc                                  <  2:3171390/148‑1 (MQ=255)
                                                                                                                 aCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGt                               <  2:3484806/148‑1 (MQ=255)
                                                                                                                 aCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAAATTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGt                               >  2:1949539/1‑148 (MQ=255)
                                                                                                                  cGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                              <  1:3130481/148‑1 (MQ=255)
                                                                                                                  cGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                              <  1:1354506/148‑1 (MQ=255)
                                                                                                                     tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCAc                           <  2:1670204/148‑1 (MQ=255)
                                                                                                                     tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCAc                           <  2:1112290/148‑1 (MQ=255)
                                                                                                                       ctcctcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCGGCAATTTGCCCGCACTCATTACATCACCCCACCCAACGCTTAATCCAGCAACAGACGCTGCAAATCGGCCTCATCGCACGCCCCCAGTTCACGc                         >  1:1780190/1‑148 (MQ=255)
                                                                                                                         cctcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCAt                       >  1:665933/1‑148 (MQ=255)
                                                                                                                            cGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTGCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                    >  1:1566796/1‑148 (MQ=255)
                                                                                                                              gATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCCCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgcg                  >  2:356990/1‑148 (MQ=255)
                                                                                                                                     gTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAg           <  1:3010199/148‑1 (MQ=255)
                                                                                                                                      ttGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGt          <  1:1898451/148‑1 (MQ=255)
                                                                                                                                      ttGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGt          >  2:899152/1‑148 (MQ=255)
                                                                                                                                              gTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAg  <  2:1571870/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAG  >  CP009273/4571088‑4571377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑