Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 40,957 C→A 93.4% V325V (GTG→GTT)  caiT ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927340,9570CA93.4% 557.3 / 30.4 196V325V (GTG→GTT) caiTputative transporter
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base A (91/92);  total (96/100)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 205 total aligned reads displayed.

TCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGC  >  CP009273/40812‑41102
                                                                                                                                                 |                                                                                                                                                 
tcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaac                                                                                                                                                 <  2:652379/148‑1 (MQ=255)
  aaCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccc                                                                                                                                               <  2:139591/148‑1 (MQ=255)
   aCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                              >  1:1335434/1‑148 (MQ=255)
   aCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                              >  2:3475958/1‑148 (MQ=255)
    cagcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacccac                                                                                                                                             >  1:3495052/1‑148 (MQ=255)
     agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacccacc                                                                                                                                            >  2:1484768/1‑148 (MQ=255)
       cagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                          >  1:263872/1‑148 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACTCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  1:595808/1‑148 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       <  1:1060754/148‑1 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  1:3566781/1‑148 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  2:245207/1‑148 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  1:1478948/1‑148 (MQ=255)
              ttACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGt                                                                                                                                   <  2:2552893/148‑1 (MQ=255)
              ttACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGt                                                                                                                                   <  2:449561/148‑1 (MQ=255)
              ttACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGt                                                                                                                                   <  1:1984379/148‑1 (MQ=255)
                 ctacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGa                                                                                                                                <  1:315564/148‑1 (MQ=255)
                 ctacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGa                                                                                                                                <  2:1945230/148‑1 (MQ=255)
                  tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGaa                                                                                                                               >  1:1634118/1‑148 (MQ=255)
                  tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGaa                                                                                                                               <  2:1315740/148‑1 (MQ=255)
                    ccGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAaca                                                                                                                             >  2:1730582/1‑148 (MQ=255)
                     cGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAacac                                                                                                                            <  2:3456321/148‑1 (MQ=255)
                      gAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACg                                                                                                                           <  1:1258436/148‑1 (MQ=255)
                         tACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTc                                                                                                                        >  1:492390/1‑148 (MQ=255)
                          aCAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTcc                                                                                                                       >  2:1407345/1‑148 (MQ=255)
                          aCAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTcc                                                                                                                       >  2:1854925/1‑148 (MQ=255)
                               ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGccc                                                                                                                  <  1:2359649/148‑1 (MQ=255)
                                  caGGATCCAGGTTGACGCTGTCAGCCACAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                                                                                                               >  2:31796/1‑148 (MQ=255)
                                  caGGATCCAGGTTGACGCTGTCAGCCACAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                                                                                                               >  2:25249/1‑148 (MQ=255)
                                      aTCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGa                                                                                                           <  1:2302990/148‑1 (MQ=255)
                                      aTCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGa                                                                                                           <  1:896359/148‑1 (MQ=255)
                                        ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                         >  1:2252055/1‑148 (MQ=255)
                                        ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                         <  2:1310795/148‑1 (MQ=255)
                                            gTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCCCAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  2:3499994/1‑148 (MQ=255)
                                            gTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  2:2157957/1‑148 (MQ=255)
                                             ttGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc                                                                                                    >  1:2601203/1‑148 (MQ=255)
                                                aCGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt                                                                                                 <  2:1461703/148‑1 (MQ=255)
                                                aCGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt                                                                                                 <  1:3378025/148‑1 (MQ=255)
                                                 cGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTa                                                                                                <  2:1248402/148‑1 (MQ=255)
                                                       cAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATg                                                                                          >  2:351907/1‑148 (MQ=255)
                                                       cAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATg                                                                                          >  2:796191/1‑148 (MQ=255)
                                                         gCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATggg                                                                                        >  2:585923/1‑148 (MQ=255)
                                                          ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                       >  2:468437/1‑148 (MQ=255)
                                                            ccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGTGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATc                                                                                     <  2:2261433/148‑1 (MQ=255)
                                                            ccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATc                                                                                     <  2:2494026/148‑1 (MQ=255)
                                                             cAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                    <  1:2132778/148‑1 (MQ=255)
                                                               gCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGt                                                                                  <  2:2147894/148‑1 (MQ=255)
                                                               gCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGt                                                                                  <  1:1012525/148‑1 (MQ=255)
                                                                   cATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAg                                                                              <  1:1152402/148‑1 (MQ=255)
                                                                    aTGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGAAACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGa                                                                             >  1:2569853/1‑148 (MQ=255)
                                                                      gCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaac                                                                           >  2:1532909/1‑148 (MQ=255)
                                                                        cGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaa                                                                         >  1:2462940/1‑148 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                        <  2:104618/148‑1 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                        <  1:3025451/148‑1 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                        <  2:2250975/148‑1 (MQ=255)
                                                                          aaGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAACTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       <  2:1524370/148‑1 (MQ=255)
                                                                          aaGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       <  1:3512399/148‑1 (MQ=255)
                                                                          aaGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       <  2:404079/148‑1 (MQ=255)
                                                                          aaGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       >  2:3415389/1‑148 (MQ=255)
                                                                           aGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACAt                                                                      >  1:1465488/1‑148 (MQ=255)
                                                                              acaGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcg                                                                   <  1:690848/148‑1 (MQ=255)
                                                                              acaGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcg                                                                   <  1:1486613/148‑1 (MQ=255)
                                                                                aGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGcgcg                                                                 >  1:3333088/1‑148 (MQ=255)
                                                                                   tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTAGAGAACAACATGCGCGGCa                                                              >  2:759228/1‑148 (MQ=255)
                                                                                     aCGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGa                                                            <  1:1531936/148‑1 (MQ=255)
                                                                                            gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                     <  1:646538/148‑1 (MQ=255)
                                                                                                gACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCaa                                                 <  2:1465488/148‑1 (MQ=255)
                                                                                                  ccACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACa                                               >  1:1653740/1‑148 (MQ=255)
                                                                                                  ccACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACa                                               >  2:3190787/1‑148 (MQ=255)
                                                                                                        gagaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCa                                         >  1:840366/1‑148 (MQ=255)
                                                                                                           aTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCg                                      >  2:1590099/1‑148 (MQ=255)
                                                                                                           aTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCg                                      >  1:1723561/1‑148 (MQ=255)
                                                                                                           aTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCg                                      >  2:2824746/1‑148 (MQ=255)
                                                                                                            tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGa                                     <  2:1335434/148‑1 (MQ=255)
                                                                                                            tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGa                                     >  2:3229826/1‑148 (MQ=255)
                                                                                                             gCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                    <  1:660956/148‑1 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                   <  2:503620/148‑1 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                   >  1:1697262/1‑148 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                   <  1:947069/148‑1 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                   <  1:1532909/148‑1 (MQ=255)
                                                                                                                ggCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCg                                 <  2:1143058/148‑1 (MQ=255)
                                                                                                                  cGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGt                               >  1:1876279/1‑148 (MQ=255)
                                                                                                                     ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                            >  1:1411258/1‑148 (MQ=255)
                                                                                                                     ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                            >  1:3422724/1‑148 (MQ=255)
                                                                                                                      gAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAag                           >  1:1138457/1‑148 (MQ=255)
                                                                                                                          aTACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGACTCGGTGAagtagt                       >  2:2018037/1‑148 (MQ=255)
                                                                                                                            aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                     <  2:3333088/148‑1 (MQ=255)
                                                                                                                            aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                     <  2:1564383/148‑1 (MQ=255)
                                                                                                                             cTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCa                    <  1:1765637/148‑1 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                   >  1:2540623/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                   >  1:753650/1‑148 (MQ=255)
                                                                                                                               cATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatg                  <  1:1960747/148‑1 (MQ=255)
                                                                                                                                     gATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg            <  2:3203930/148‑1 (MQ=255)
                                                                                                                                     gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg            >  2:2316711/1‑148 (MQ=255)
                                                                                                                                     gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg            >  1:2725045/1‑148 (MQ=255)
                                                                                                                                        aGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTg         >  1:2231345/1‑148 (MQ=255)
                                                                                                                                            tAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAc     >  1:2611703/1‑148 (MQ=255)
                                                                                                                                            tAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAc     >  1:3130841/1‑148 (MQ=255)
                                                                                                                                              aaTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCg   <  2:2064259/148‑1 (MQ=255)
                                                                                                                                              aaTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCg   <  2:263872/148‑1 (MQ=255)
                                                                                                                                               aTCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGc  <  2:3008488/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGC  >  CP009273/40812‑41102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑