Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,255 A→C 100% L95L (CTT→CTG)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,2550AC96.5% 784.2 / 5.0 200L95L (CTT→CTG) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base C (107/86);  total (111/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 202 total aligned reads displayed.

TCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAAT  >  CP009273/4584109‑4584399
                                                                                                                                                  |                                                                                                                                                
tCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGaa                                                                                                                                                 >  1:3283034/1‑148 (MQ=255)
   ccAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGtt                                                                                                                                              <  1:2799235/148‑1 (MQ=255)
      aTCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgcg                                                                                                                                           >  1:1929150/1‑148 (MQ=255)
       tCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgcgc                                                                                                                                          >  1:1158134/1‑148 (MQ=255)
        ccccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCa                                                                                                                                         >  2:646738/1‑148 (MQ=255)
          ccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAt                                                                                                                                       >  2:184838/1‑148 (MQ=255)
            aTACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATcc                                                                                                                                     >  2:482598/1‑148 (MQ=255)
            aTACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATcc                                                                                                                                     >  2:2464850/1‑148 (MQ=255)
                cGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTa                                                                                                                                 <  1:3149018/148‑1 (MQ=255)
                    aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                             >  1:1131732/1‑148 (MQ=255)
                    aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                             <  1:1341942/148‑1 (MQ=255)
                    aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                             >  1:1513474/1‑148 (MQ=255)
                    aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                             >  1:1513739/1‑148 (MQ=255)
                     cGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGa                                                                                                                            <  2:1650478/148‑1 (MQ=255)
                       cACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGc                                                                                                                              <  1:3522293/144‑1 (MQ=255)
                          cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGaa                                                                                                                       >  1:1856990/1‑148 (MQ=255)
                            acacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                     >  1:293369/1‑148 (MQ=255)
                            acacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                     >  1:3041209/1‑148 (MQ=255)
                            acacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                     >  1:3319098/1‑148 (MQ=255)
                             cacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAac                                                                                                                    <  1:802931/148‑1 (MQ=255)
                             cacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAac                                                                                                                    <  1:2590096/148‑1 (MQ=255)
                               cGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAacac                                                                                                                  >  1:3223937/1‑148 (MQ=255)
                                 aaCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGCACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGGAACCACgg                                                                                                                >  2:2518427/1‑148 (MQ=255)
                                     gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                            <  2:2541048/148‑1 (MQ=255)
                                     gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                            <  1:3128149/148‑1 (MQ=255)
                                     gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                            <  1:1818240/148‑1 (MQ=255)
                                        tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                         <  2:1367417/148‑1 (MQ=255)
                                        tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                         <  2:1161336/148‑1 (MQ=255)
                                        tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                         <  1:3442657/148‑1 (MQ=255)
                                         aaaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaaa                                                                                                        >  2:2548403/1‑148 (MQ=255)
                                         aaaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaaa                                                                                                        >  1:2727288/1‑148 (MQ=255)
                                           aGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaaa                                                                                                        >  1:1245780/1‑146 (MQ=255)
                                           aGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgc                                                                                                      >  2:1875119/1‑148 (MQ=255)
                                            gTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcg                                                                                                     <  2:1929150/148‑1 (MQ=255)
                                                tgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGa                                                                                                 <  1:2044123/148‑1 (MQ=255)
                                                  cACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAtt                                                                                               >  1:3527738/1‑148 (MQ=255)
                                                   aCCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAttt                                                                                              <  2:3235651/148‑1 (MQ=255)
                                                       tGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGt                                                                                          <  2:2974654/148‑1 (MQ=255)
                                                         ccAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCCGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTgg                                                                                        <  1:3071333/148‑1 (MQ=255)
                                                           aGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCa                                                                                      >  2:1115556/1‑148 (MQ=255)
                                                            gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                     <  1:1433727/148‑1 (MQ=255)
                                                               aTTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTa                                                                                  >  1:1780392/1‑148 (MQ=255)
                                                                ttGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTaa                                                                                 >  2:2404914/1‑148 (MQ=255)
                                                                   ccTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGGGTGGGCCTTTACGATCCAATCGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                              <  1:450930/148‑1 (MQ=255)
                                                                   ccTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                              >  2:333473/1‑148 (MQ=255)
                                                                     tGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGATAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                            <  2:2893050/148‑1 (MQ=255)
                                                                      ggAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCac                                                                           >  2:1032833/1‑147 (MQ=255)
                                                                         aCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCACGGCAGTTGCTCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                        <  1:1586509/148‑1 (MQ=255)
                                                                         aCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                        <  1:3058172/148‑1 (MQ=255)
                                                                            gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttcttc                                                                     <  2:2736060/148‑1 (MQ=255)
                                                                            gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttcttc                                                                     <  2:3542520/148‑1 (MQ=255)
                                                                            gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttcttc                                                                     <  2:1780392/148‑1 (MQ=255)
                                                                                ccAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGa                                                                 <  2:2359330/148‑1 (MQ=255)
                                                                                 cAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGaa                                                                <  1:2155341/148‑1 (MQ=255)
                                                                                  agaagaACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAAt                                                               <  2:8542/148‑1 (MQ=255)
                                                                                   gaagaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATc                                                              >  1:290685/1‑148 (MQ=255)
                                                                                      gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGtt                                                           >  2:2843652/1‑148 (MQ=255)
                                                                                       aaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                          <  1:239526/148‑1 (MQ=255)
                                                                                          aTCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGTAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAt                                                       <  2:1158134/148‑1 (MQ=255)
                                                                                          aTCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAt                                                       <  1:200019/148‑1 (MQ=255)
                                                                                           tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAtt                                                      <  1:2361676/148‑1 (MQ=255)
                                                                                           tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAtt                                                      >  1:1642739/1‑148 (MQ=255)
                                                                                           tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAtt                                                      >  2:1499841/1‑148 (MQ=255)
                                                                                            ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                     >  1:2531927/1‑148 (MQ=255)
                                                                                              ccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGc                                                   >  2:1260381/1‑148 (MQ=255)
                                                                                               cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                  >  2:2625021/1‑148 (MQ=255)
                                                                                                  tCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAcc                                               >  1:489635/1‑148 (MQ=255)
                                                                                                     cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                            >  1:1753227/1‑148 (MQ=255)
                                                                                                        cATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACCGc                                         >  2:2211783/1‑148 (MQ=255)
                                                                                                        cATCAGGCGTGGGCCTATACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                         >  2:2754781/1‑148 (MQ=255)
                                                                                                          tCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGa                                       <  2:3520306/148‑1 (MQ=255)
                                                                                                           cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                      <  1:843073/148‑1 (MQ=255)
                                                                                                           cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                      <  1:1195503/148‑1 (MQ=255)
                                                                                                           cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                      >  2:1250721/1‑148 (MQ=255)
                                                                                                             ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACt                                    >  1:1023709/1‑148 (MQ=255)
                                                                                                             ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACt                                    >  1:3327250/1‑148 (MQ=255)
                                                                                                              gCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                   >  2:578040/1‑148 (MQ=255)
                                                                                                              gCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                   >  2:1245244/1‑148 (MQ=255)
                                                                                                                      cTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgag                           <  2:1885050/148‑1 (MQ=255)
                                                                                                                       tttACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                          >  1:367150/1‑148 (MQ=255)
                                                                                                                         tACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATa                        <  1:2371893/148‑1 (MQ=255)
                                                                                                                         tACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATa                        <  1:58281/148‑1 (MQ=255)
                                                                                                                          aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATaa                       <  1:1136678/148‑1 (MQ=255)
                                                                                                                          aCGATCCAATAGTGGGCCGCAAGGCAGTGGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAACGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATaa                       <  1:723949/148‑1 (MQ=255)
                                                                                                                           cGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                      >  2:2315574/1‑148 (MQ=255)
                                                                                                                             aTCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttt                    >  1:743746/1‑147 (MQ=255)
                                                                                                                              tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttta                   >  2:623183/1‑146 (MQ=255)
                                                                                                                               ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                  >  1:952621/1‑148 (MQ=255)
                                                                                                                                    tgTGGGCCGCAAGTCAGTTGCGCATTCCCGTAAGCGAGTGAAAACACGGAGATCAAAGCGACTATTTCGGTGTCACTTTATCCCTATTCTTCAAGAATCGTTAAATTTGATCCCTTCATCGAACTGCTGTCGAGATAATTTATTTccg             <  2:539629/147‑1 (MQ=255)
                                                                                                                                    aGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccg             >  1:3103769/1‑148 (MQ=255)
                                                                                                                                      tGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgcc           >  2:902801/1‑148 (MQ=255)
                                                                                                                                        ggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCTGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACcgccgc         >  1:902695/1‑148 (MQ=255)
                                                                                                                                         gCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCa        >  2:3046533/1‑148 (MQ=255)
                                                                                                                                          ccGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaa       <  2:567529/148‑1 (MQ=255)
                                                                                                                                          ccGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaa       >  2:836271/1‑148 (MQ=255)
                                                                                                                                           cGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaa      >  1:2196205/1‑148 (MQ=255)
                                                                                                                                             cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa    <  1:2296862/148‑1 (MQ=255)
                                                                                                                                             cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACAGCCGCaaaaa    <  1:2509371/148‑1 (MQ=255)
                                                                                                                                             cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACAGCCGCaaaaa    <  1:2509283/148‑1 (MQ=255)
                                                                                                                                               aGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaat  >  2:82183/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAAT  >  CP009273/4584109‑4584399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑