Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,585,338 A→T 100% S111T (TCT→ACT)  yjjM ← yjjMN operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,585,3380AT100.0% 703.5 / NA 213S111T (TCT→ACT) yjjMyjjMN operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (103/110);  total (103/110)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

TGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGT  >  CP009273/4585193‑4585481
                                                                                                                                                 |                                                                                                                                               
      gCGTAGGATTGATCTAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGttt                                                                                                                                         <  2:3128353/148‑1 (MQ=255)
      gCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGttt                                                                                                                                         >  2:1798095/1‑148 (MQ=255)
      gCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGttt                                                                                                                                         >  2:188877/1‑148 (MQ=255)
        gTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTcc                                                                                                                                       <  1:2515228/148‑1 (MQ=255)
         tAGGATTGATCTAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTccc                                                                                                                                      >  2:391224/1‑148 (MQ=255)
         tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTccc                                                                                                                                      <  1:1443234/148‑1 (MQ=255)
          aGGATTGATCTAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTcccc                                                                                                                                     >  2:1872476/1‑148 (MQ=255)
           ggATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCt                                                                                                                                    >  1:2638532/1‑148 (MQ=255)
            ttttGATGGATTTGCTTCTTTCTCCACTGGCCTCGCTTTTCGCTGTGATTAAGTTTATACCGGCCGATTTTTAAAAGATATTCTCTTACTACTACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTg                                                                                                                                   <  1:853257/146‑1 (MQ=255)
            gATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTg                                                                                                                                   >  2:2195582/1‑148 (MQ=255)
             aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTgg                                                                                                                                  >  1:866828/1‑148 (MQ=255)
               tGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGc                                                                                                                                >  1:1996319/1‑148 (MQ=255)
                  tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                             >  2:3193141/1‑148 (MQ=255)
                      aTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTg                                                                                                                         <  1:2956541/148‑1 (MQ=255)
                       ttGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTgg                                                                                                                        >  1:1165531/1‑148 (MQ=255)
                         gCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGgcg                                                                                                                      <  2:1328585/148‑1 (MQ=255)
                             cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTgg                                                                                                                  <  2:744879/148‑1 (MQ=255)
                              aTGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGt                                                                                                                 <  1:241191/148‑1 (MQ=255)
                                gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGttt                                                                                                               <  2:2240890/148‑1 (MQ=255)
                                gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGttt                                                                                                               <  2:1739398/148‑1 (MQ=255)
                                gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGttt                                                                                                               <  2:1438161/148‑1 (MQ=255)
                                   ccACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatc                                                                                                            >  1:619769/1‑148 (MQ=255)
                                   ccACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatc                                                                                                            >  1:1604428/1‑148 (MQ=255)
                                    cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatca                                                                                                           <  1:3128590/148‑1 (MQ=255)
                                    cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTGACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatca                                                                                                           <  2:3416062/148‑1 (MQ=255)
                                     aCTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatcat                                                                                                          >  2:2002910/1‑148 (MQ=255)
                                      cTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatcatc                                                                                                         >  2:625320/1‑148 (MQ=255)
                                       tGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCg                                                                                                        <  1:2882712/148‑1 (MQ=255)
                                       tGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCg                                                                                                        >  2:2752248/1‑148 (MQ=255)
                                         gCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTg                                                                                                      >  1:3412290/1‑148 (MQ=255)
                                             cgcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGaaaa                                                                                                  >  2:1103916/1‑148 (MQ=255)
                                             cgcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGaaaa                                                                                                  >  1:679827/1‑148 (MQ=255)
                                                 tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGc                                                                                              >  2:1505753/1‑148 (MQ=255)
                                                    cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                           <  2:1016850/148‑1 (MQ=255)
                                                    cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                           <  2:1016780/148‑1 (MQ=255)
                                                          gAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa                                                                                     <  2:1173171/148‑1 (MQ=255)
                                                           aaTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAac                                                                                    >  2:3182819/1‑148 (MQ=255)
                                                           aaTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAac                                                                                    <  2:2663705/148‑1 (MQ=255)
                                                              aaGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                 >  1:2389948/1‑148 (MQ=255)
                                                              aaGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                 >  2:3026571/1‑148 (MQ=255)
                                                              aaGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                 >  2:2427409/1‑148 (MQ=255)
                                                               aGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtt                                                                                >  1:349060/1‑148 (MQ=255)
                                                                 aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                              <  1:205846/148‑1 (MQ=255)
                                                                  ttATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgt                                                                             <  2:256115/148‑1 (MQ=255)
                                                                   tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                            <  1:392444/148‑1 (MQ=255)
                                                                   tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                            <  1:2185368/148‑1 (MQ=255)
                                                                   tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                            <  2:710025/148‑1 (MQ=255)
                                                                     taACGTCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtttt                                                                          <  2:3441860/148‑1 (MQ=255)
                                                                        cGGCCGAATTTTAAAAGGTATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                       <  1:1815533/148‑1 (MQ=255)
                                                                            cGAATTTTAAAAGATAGTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACt                                                                   <  1:2822030/148‑1 (MQ=255)
                                                                              aaTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                 <  2:1230764/148‑1 (MQ=255)
                                                                              aaTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                 >  2:1652070/1‑148 (MQ=255)
                                                                              aaTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                 <  2:194382/148‑1 (MQ=255)
                                                                               aTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAt                                                                >  1:2774430/1‑148 (MQ=255)
                                                                                ttttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAATGCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                               >  2:3236870/1‑148 (MQ=255)
                                                                                ttttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                               >  1:798679/1‑148 (MQ=255)
                                                                                  ttAAAAGATAGTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa                                                             <  2:3525138/148‑1 (MQ=255)
                                                                                    aaaaGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                           <  2:3011194/148‑1 (MQ=255)
                                                                                      aaGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCg                                                         <  2:444871/148‑1 (MQ=255)
                                                                                       aGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCgg                                                        >  1:2839827/1‑148 (MQ=255)
                                                                                        gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCgat                                                       >  1:486070/1‑146 (MQ=255)
                                                                                        gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                       >  2:3522360/1‑148 (MQ=255)
                                                                                         atatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgta                                                      >  2:2713359/1‑148 (MQ=255)
                                                                                             tctcTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTAc                                                  >  1:3327653/1‑148 (MQ=255)
                                                                                              ctctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                 <  1:1917868/148‑1 (MQ=255)
                                                                                              ctctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                 <  2:1352262/148‑1 (MQ=255)
                                                                                              ctctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTATCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                 >  1:648104/1‑148 (MQ=255)
                                                                                               tctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAg                                                >  1:3005792/1‑148 (MQ=255)
                                                                                                ctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                               <  1:2215926/148‑1 (MQ=255)
                                                                                                     tacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                          <  1:2301255/148‑1 (MQ=255)
                                                                                                     tacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                          <  1:1731271/148‑1 (MQ=255)
                                                                                                     tacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                          >  1:387949/1‑148 (MQ=255)
                                                                                                         cccGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGt                                      <  2:648235/147‑1 (MQ=255)
                                                                                                         aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGt                                      <  2:801822/148‑1 (MQ=255)
                                                                                                         aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTGTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGt                                      <  2:802184/148‑1 (MQ=255)
                                                                                                           cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtca                                    <  1:1103916/148‑1 (MQ=255)
                                                                                                           cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtca                                    <  1:1107487/148‑1 (MQ=255)
                                                                                                             ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                  <  2:2429263/148‑1 (MQ=255)
                                                                                                             ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                  >  1:3052646/1‑148 (MQ=255)
                                                                                                               cTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTcatcat                                <  1:952210/148‑1 (MQ=255)
                                                                                                               cTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTcatcat                                <  2:1058740/148‑1 (MQ=255)
                                                                                                                 gACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATgg                              >  2:2049533/1‑148 (MQ=255)
                                                                                                                   cacCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTc                            >  1:172950/1‑148 (MQ=255)
                                                                                                                      ccGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAgg                         <  2:2839853/148‑1 (MQ=255)
                                                                                                                       cgcgGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGt                        <  1:774615/148‑1 (MQ=255)
                                                                                                                        gcgGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGtt                       <  2:2529555/148‑1 (MQ=255)
                                                                                                                              cGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcgcg                 >  2:2583785/1‑148 (MQ=255)
                                                                                                                               gAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACgcgc                <  2:481466/148‑1 (MQ=255)
                                                                                                                                  ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             >  1:2959511/1‑148 (MQ=255)
                                                                                                                                  ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             >  1:2674827/1‑148 (MQ=255)
                                                                                                                                  ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             >  2:3007245/1‑148 (MQ=255)
                                                                                                                                  ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             <  2:798177/148‑1 (MQ=255)
                                                                                                                                   cAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATa            <  2:2492835/148‑1 (MQ=255)
                                                                                                                                     gTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAAc          >  2:1289133/1‑148 (MQ=255)
                                                                                                                                      ttGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACa         >  1:886446/1‑148 (MQ=255)
                                                                                                                                       tGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACAt        >  1:1389396/1‑148 (MQ=255)
                                                                                                                                         cAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAg      <  2:1665134/148‑1 (MQ=255)
                                                                                                                                            ttCAGTAAACGTTGGCCCTGGGGGTTTCTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAATCACTTTGTTTTGTTCACTCATTGAAGCTGTAGTACTGGCGAAACCTTCATCATGGTCAGGTTTACGCGCAATAACATAGTCg   <  1:1329965/148‑1 (MQ=255)
                                                                                                                                             tCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGt  <  1:3182819/148‑1 (MQ=255)
                                                                                                                                             tCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGt  <  2:2095829/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGT  >  CP009273/4585193‑4585481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑