Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,589,130 G→T 100% I43I (ATC→ATA)  opgB ← phosphoglycerol transferases I and II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,589,1300GT98.9% 587.5 / ‑3.2 185I43I (ATC→ATA) opgBphosphoglycerol transferases I and II
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (89/93);  total (91/94)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

GAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTA  >  CP009273/4588986‑4589274
                                                                                                                                                |                                                                                                                                                
gAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTtata                                                                                                                                               <  2:3150399/148‑1 (MQ=255)
 aaCACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTatat                                                                                                                                              <  2:38189/148‑1 (MQ=255)
  acacCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATAtt                                                                                                                                             <  2:2144393/148‑1 (MQ=255)
  acacCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATAtt                                                                                                                                             <  1:889220/148‑1 (MQ=255)
    acCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTa                                                                                                                                           <  2:938529/148‑1 (MQ=255)
     ccGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaa                                                                                                                                          <  2:3323817/148‑1 (MQ=255)
      cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         <  1:1480094/148‑1 (MQ=255)
      cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         <  2:789942/148‑1 (MQ=255)
      cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         <  1:3251675/148‑1 (MQ=255)
      cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         <  2:2577498/148‑1 (MQ=255)
      cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         <  2:2551629/148‑1 (MQ=255)
         tGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACg                                                                                                                                      <  2:3327401/148‑1 (MQ=255)
          gTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGa                                                                                                                                     <  2:722944/148‑1 (MQ=255)
           tCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGAc                                                                                                                                    >  2:2079740/1‑148 (MQ=255)
               ccccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAAt                                                                                                                                <  2:932747/148‑1 (MQ=255)
                     cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATCGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGcccc                                                                                                                          <  2:316638/148‑1 (MQ=255)
                     cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGcccc                                                                                                                          >  1:1077803/1‑148 (MQ=255)
                     cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGcccc                                                                                                                          >  1:2253742/1‑148 (MQ=255)
                     cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAAACGGGTGTTATTTAAACCGACAAATTCcccc                                                                                                                          >  1:1332584/1‑148 (MQ=255)
                         aTGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCa                                                                                                                      <  2:3233699/148‑1 (MQ=255)
                          tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCAc                                                                                                                     >  1:1979743/1‑148 (MQ=255)
                           gCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCAcc                                                                                                                    >  1:3111241/1‑148 (MQ=255)
                            ccGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCg                                                                                                                   >  1:3481463/1‑148 (MQ=255)
                                tACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTaa                                                                                                               >  1:1069264/1‑148 (MQ=255)
                                 aCCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAAc                                                                                                              >  1:3546335/1‑148 (MQ=255)
                                  cccGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACg                                                                                                             <  1:2443527/148‑1 (MQ=255)
                                    cGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTg                                                                                                           <  2:2890343/148‑1 (MQ=255)
                                     ggCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTgg                                                                                                          <  1:528634/148‑1 (MQ=255)
                                     ggCAAAATGTATTGGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTgg                                                                                                          <  2:966634/148‑1 (MQ=255)
                                      gaaaaaTTTATTTGCTGACGCCAGCACCGGTCAGGGTGTTTGTTAAGTTATAGAGAACCGCGTCTTTAATCCCATCGCCAGTAAAATAGTCGCTGGAAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGc                                                                                                         <  1:617839/146‑1 (MQ=255)
                                             gTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAac                                                                                                  <  2:2470625/148‑1 (MQ=255)
                                                 ttGCTGACGCTAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc                                                                                              <  2:1354908/148‑1 (MQ=255)
                                                 ttGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc                                                                                              <  2:3423732/148‑1 (MQ=255)
                                                       aCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATTTTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGgtgt                                                                                        <  1:1180905/148‑1 (MQ=255)
                                                        cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCTTTAATACAATCGCCAGTAAAATAGTCGCTGGAAAACAGGTTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGtt                                                                                       <  1:415994/148‑1 (MQ=255)
                                                        cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGtt                                                                                       >  2:2453727/1‑148 (MQ=255)
                                                        cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGtt                                                                                       >  1:1659570/1‑148 (MQ=255)
                                                           cAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACg                                                                                    <  2:2256174/148‑1 (MQ=255)
                                                           cAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACg                                                                                    >  2:2884651/1‑148 (MQ=255)
                                                             gCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTc                                                                                  <  2:1143201/148‑1 (MQ=255)
                                                             gCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTc                                                                                  >  2:558205/1‑148 (MQ=255)
                                                             gCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGATTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTc                                                                                  >  1:2601500/1‑148 (MQ=255)
                                                              cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTcc                                                                                 >  1:1877957/1‑148 (MQ=255)
                                                                ccGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCCGGTGTTACGTCCCg                                                                               >  1:1255716/1‑148 (MQ=255)
                                                                ccGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCg                                                                               <  2:899184/148‑1 (MQ=255)
                                                                  ggTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCt                                                                             >  2:1011947/1‑148 (MQ=255)
                                                                   gTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCtt                                                                            >  1:2923864/1‑148 (MQ=255)
                                                                    tCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCttt                                                                           >  2:3096464/1‑148 (MQ=255)
                                                                      aGGCTGTTGTTTAAGTTTTAGAGAACCGCGGCTTTAATAGCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCTTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTcc                                                                         <  2:3474683/148‑1 (MQ=255)
                                                                      aGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTcc                                                                         <  2:1763860/148‑1 (MQ=255)
                                                                       ggCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCa                                                                        >  1:2482913/1‑148 (MQ=255)
                                                                           gTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCg                                                                    >  1:2290937/1‑148 (MQ=255)
                                                                           gTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCg                                                                    >  1:1415937/1‑148 (MQ=255)
                                                                              ggTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTaa                                                                 <  2:1844192/148‑1 (MQ=255)
                                                                              ggTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTaa                                                                 <  1:582839/148‑1 (MQ=255)
                                                                                  aaGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCa                                                             >  2:3413541/1‑148 (MQ=255)
                                                                                  aaGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCa                                                             >  2:1645887/1‑148 (MQ=255)
                                                                                    ggTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGc                                                           >  1:2292246/1‑148 (MQ=255)
                                                                                    ggTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGc                                                           >  2:1274197/1‑148 (MQ=255)
                                                                                        taGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACag                                                       <  2:1979743/148‑1 (MQ=255)
                                                                                        taGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACag                                                       <  2:2923864/148‑1 (MQ=255)
                                                                                         agagAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACaga                                                      <  2:2972492/148‑1 (MQ=255)
                                                                                         agagAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAAAGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACaga                                                      >  1:933408/1‑148 (MQ=255)
                                                                                          gagaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                                     <  2:2209418/148‑1 (MQ=255)
                                                                                          gagaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                                     <  2:3292193/148‑1 (MQ=255)
                                                                                           agaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAgg                                                    <  1:2192994/148‑1 (MQ=255)
                                                                                            gaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGc                                                   >  2:2527341/1‑148 (MQ=255)
                                                                                            gaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGc                                                   >  2:2527500/1‑148 (MQ=255)
                                                                                               ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCgag                                                >  2:1155070/1‑148 (MQ=255)
                                                                                                cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCgaga                                               <  2:2745812/148‑1 (MQ=255)
                                                                                                cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCgaga                                               <  2:2745417/148‑1 (MQ=255)
                                                                                                 gcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaa                                              >  2:747658/1‑148 (MQ=255)
                                                                                                   gtcttTAATACCATCGCCTTTAAAATAGTGGCTGGAAAAAAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaaa                                            <  1:3535/148‑1 (MQ=255)
                                                                                                    tcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaaaa                                           >  1:76018/1‑148 (MQ=255)
                                                                                                     cgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAg                                          >  2:476121/1‑148 (MQ=255)
                                                                                                       ttAATACCTTCGCAAGTAAAATAGTCGCTGGAAAACTGGTTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                                        <  1:812525/148‑1 (MQ=255)
                                                                                                       ttAATACCATCGCCAGTAAAATAGTCGCTGGAAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                                        <  1:1223320/148‑1 (MQ=255)
                                                                                                        tAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGc                                       >  2:168278/1‑148 (MQ=255)
                                                                                                          aTACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGa                                     >  2:1175722/1‑148 (MQ=255)
                                                                                                            aCCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGaaa                                   >  1:216899/1‑148 (MQ=255)
                                                                                                             ccATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAag                                  >  2:3511618/1‑148 (MQ=255)
                                                                                                             ccATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAag                                  >  2:3511835/1‑148 (MQ=255)
                                                                                                              cATCGCCAGTAAAATAGTCGCTGGAAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAaga                                 <  1:1263691/148‑1 (MQ=255)
                                                                                                                 cGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTc                                                                          <  2:2713344/104‑1 (MQ=255)
                                                                                                                 cGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTc                                                                          >  1:2713344/1‑104 (MQ=255)
                                                                                                                 cGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagagag                              <  2:3285864/148‑1 (MQ=255)
                                                                                                                     aGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGagtagt                          >  2:279692/1‑148 (MQ=255)
                                                                                                                      gTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt                         <  2:3111241/148‑1 (MQ=255)
                                                                                                                       tAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTc                        <  2:980574/148‑1 (MQ=255)
                                                                                                                        aaaaTAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCt                       >  1:2172703/1‑148 (MQ=255)
                                                                                                                        aaaaTAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCt                       <  2:858475/148‑1 (MQ=255)
                                                                                                                             aGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACaa                  >  2:3559478/1‑148 (MQ=255)
                                                                                                                              gTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAAc                 >  2:1828581/1‑148 (MQ=255)
                                                                                                                               tCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACg                >  1:1517933/1‑148 (MQ=255)
                                                                                                                                cGCTGGCAAACAGGGTTATATTTAAGACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAGAGGGCGAAAGAGAGTAGTTCTGACAACgg               >  2:1176098/1‑148 (MQ=255)
                                                                                                                                     gCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt          >  1:176488/1‑148 (MQ=255)
                                                                                                                                       aaaCAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATcc        <  2:1877957/148‑1 (MQ=255)
                                                                                                                                         aCAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTc      >  2:3471484/1‑148 (MQ=255)
                                                                                                                                         aCAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTc      >  1:330921/1‑148 (MQ=255)
                                                                                                                                           aGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCAc    <  2:402195/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTA  >  CP009273/4588986‑4589274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑