BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,611,666	G→T	8.5%	A27A (GCG→GCT)	yjjJ →	putative protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,611,666	0	G	T	8.5%	848.8 / 33.6	235	A27A (GCG→GCT)	yjjJ	putative protein kinase
Reads supporting (aligned to +/- strand): ref base G (118/97); new base T (7/13); total (125/110)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 237 total aligned reads displayed.

CGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATT  >  CP009273/4611519‑4611812

 gCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGa                                                                                                                                                   <  1:2894953/148‑1 (MQ=255)

 gCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGa                                                                                                                                                   <  2:2362044/148‑1 (MQ=255)

  cTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTAc                                                                                                                                                  >  2:732495/1‑148 (MQ=255)

   tataCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACg                                                                                                                                                 >  1:835598/1‑148 (MQ=255)

   tataCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACg                                                                                                                                                 >  1:835677/1‑148 (MQ=255)

    ataCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGt                                                                                                                                                >  2:1099244/1‑148 (MQ=255)

     taCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGtt                                                                                                                                               <  1:2334642/148‑1 (MQ=255)

     taCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGtt                                                                                                                                               >  1:1122656/1‑148 (MQ=255)

       cTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtct                                                                                                                                             >  2:777258/1‑148 (MQ=255)

              gTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCt                                                                                                                                      >  2:3530239/1‑148 (MQ=255)

               tGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCtt                                                                                                                                     >  2:1528864/1‑148 (MQ=255)

                gAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttg                                                                                                                                    <  2:3520724/148‑1 (MQ=255)

                gAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttg                                                                                                                                    >  2:2499676/1‑148 (MQ=255)

                gAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttg                                                                                                                                    >  1:1683971/1‑148 (MQ=255)

                gAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttg                                                                                                                                    <  2:629396/148‑1 (MQ=255)

                 aaaTGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttgt                                                                                                                                   >  2:2056646/1‑148 (MQ=255)

                   aTGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttgttg                                                                                                                                 >  1:12776/1‑148 (MQ=255)

                    tgtgTTAATAAATCTATTCAAGTATCTATTCACGAATCTTTTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGATTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGc                                                                                                                                <  1:2674302/148‑1 (MQ=255)

                    tgtgTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGc                                                                                                                                >  1:1843973/1‑148 (MQ=255)

                        ttAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCt                                                                                                                                      >  2:1251848/1‑138 (MQ=255)

                        ttAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCt                                                                                                                                      <  1:1251848/138‑1 (MQ=255)

                          aataaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAgaga                                                                                                                          >  1:3006107/1‑148 (MQ=255)

                           ataaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaa                                                                                                                         <  1:1991597/148‑1 (MQ=255)

                            taaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaag                                                                                                                        >  1:363049/1‑148 (MQ=255)

                             aaaTCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaaga                                                                                                                       <  2:1823239/148‑1 (MQ=255)

                                tCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCg                                                                                                                    >  1:2937007/1‑148 (MQ=255)

                                 cTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCgg                                                                                                                   >  1:1197178/1‑148 (MQ=255)

                                  tATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTATGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCggg                                                                                                                  <  1:122566/148‑1 (MQ=255)

                                     tCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGa                                                                                                               <  1:917438/148‑1 (MQ=255)

                                      cAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAGTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGAt                                                                                                              <  2:1697433/148‑1 (MQ=255)

                                      cAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGAt                                                                                                              <  2:1953/148‑1 (MQ=255)

                                         gTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCg                                                                                                           <  2:560463/148‑1 (MQ=255)

                                          tATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGc                                                                                                          >  2:1883809/1‑148 (MQ=255)

                                               aTTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTgg                                                                                                     <  2:940171/148‑1 (MQ=255)

                                                ttCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGt                                                                                                    <  1:1471147/148‑1 (MQ=255)

                                                  cACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTaa                                                                                                  >  2:2206/1‑148 (MQ=255)

                                                   aCGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTaaa                                                                                                 <  1:2613062/148‑1 (MQ=255)

                                                     gAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGc                                                                                               <  2:1658050/148‑1 (MQ=255)

                                                      aaTCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCa                                                                                              <  2:3079886/148‑1 (MQ=255)

                                                      aaTCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCa                                                                                              <  2:2485730/148‑1 (MQ=255)

                                                        tCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACg                                                                                            <  1:2197885/148‑1 (MQ=255)

                                                        tCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACg                                                                                            <  2:2907693/148‑1 (MQ=255)

                                                          tATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACggg                                                                                          >  1:2825161/1‑148 (MQ=255)

                                                           aTTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGc                                                                                         >  1:1510415/1‑148 (MQ=255)

                                                            ttCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCa                                                                                        <  1:3310333/148‑1 (MQ=255)

                                                            ttCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCa                                                                                        <  1:3095810/148‑1 (MQ=255)

                                                             tCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCaa                                                                                       >  1:1094702/1‑148 (MQ=255)

                                                              cATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAc                                                                                      >  1:1143244/1‑148 (MQ=255)

                                                                 tAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAcgcg                                                                                   <  1:876253/148‑1 (MQ=255)

                                                                 tAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAcgcg                                                                                   <  1:1687016/148‑1 (MQ=255)

                                                                 tAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAcgcg                                                                                   <  1:2502922/148‑1 (MQ=255)

                                                                  aaTGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGa                                                                                  <  2:1346778/148‑1 (MQ=255)

                                                                   aTGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGat                                                                                 >  1:490149/1‑148 (MQ=255)

                                                                      agcgagcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATg                                                                              <  1:1006605/148‑1 (MQ=255)

                                                                        cgagcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCa                                                                            >  2:2230557/1‑148 (MQ=255)

                                                                        cgagcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCa                                                                            >  2:3442728/1‑148 (MQ=255)

                                                                          agcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCAct                                                                          >  2:2266459/1‑148 (MQ=255)

                                                                           gcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActg                                                                         >  2:1943129/1‑148 (MQ=255)

                                                                               actgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACtgctgc                                                                     >  1:1463178/1‑148 (MQ=255)

                                                                               actgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACtgctgc                                                                     >  2:871906/1‑148 (MQ=255)

                                                                                 tgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGt                                                                   >  1:2718703/1‑148 (MQ=255)

                                                                                 tgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGt                                                                   >  2:3425018/1‑148 (MQ=255)

                                                                                  gaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTc                                                                  <  2:2829117/148‑1 (MQ=255)

                                                                                   aTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTcc                                                                 <  1:310826/148‑1 (MQ=255)

                                                                                     cTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCtt                                                               >  2:1754997/1‑148 (MQ=255)

                                                                                       tttACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTAt                                                             <  1:3265038/148‑1 (MQ=255)

                                                                                              cAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGaa                                                      <  1:1025983/148‑1 (MQ=255)

                                                                                              cAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGaa                                                      <  1:1025909/148‑1 (MQ=255)

                                                                                                 gggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTg                                                   >  2:3310720/1‑148 (MQ=255)

                                                                                                   gCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAg                                                 >  1:453066/1‑148 (MQ=255)

                                                                                                   gCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAg                                                 <  2:2904229/148‑1 (MQ=255)

                                                                                                    ccGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGc                                                >  2:1419530/1‑148 (MQ=255)

                                                                                                      gcgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGt                                              <  1:531602/148‑1 (MQ=255)

                                                                                                       cgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTa                                             >  1:99419/1‑148 (MQ=255)

                                                                                                         ttCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAtt                                           >  2:2842565/1‑148 (MQ=255)

                                                                                                          tCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTc                                          >  2:1470817/1‑148 (MQ=255)

                                                                                                            tGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTccc                                        >  2:3432160/1‑148 (MQ=255)

                                                                                                               cccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTc                                     <  2:3356373/148‑1 (MQ=255)

                                                                                                                    aaTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCg                                >  2:410221/1‑148 (MQ=255)

                                                                                                                    aaTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCg                                >  2:410547/1‑148 (MQ=255)

                                                                                                                       tGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGt                             <  1:275129/148‑1 (MQ=255)

                                                                                                                       tGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGt                             >  2:3260964/1‑148 (MQ=255)

                                                                                                                        gcgcCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTg                            <  1:2913724/148‑1 (MQ=255)

                                                                                                                         cgcCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTgg                           <  1:60379/148‑1 (MQ=255)

                                                                                                                            cAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACg                        <  1:2266459/148‑1 (MQ=255)

                                                                                                                             aGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGa                       <  1:305375/148‑1 (MQ=255)

                                                                                                                             aGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGa                       <  2:2467689/148‑1 (MQ=255)

                                                                                                                              gCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGAt                      >  1:2621937/1‑148 (MQ=255)

                                                                                                                              gCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGAt                      >  1:2086972/1‑148 (MQ=255)

                                                                                                                              gCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGAt                      >  2:2335297/1‑148 (MQ=255)

                                                                                                                               cGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATa                     <  2:3006107/148‑1 (MQ=255)

                                                                                                                               cGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATa                     >  1:2062870/1‑148 (MQ=255)

                                                                                                                                      cAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGaaa              <  2:3189062/148‑1 (MQ=255)

                                                                                                                                        aTCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAgg            <  1:410547/148‑1 (MQ=255)

                                                                                                                                        aTCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATAGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAgg            <  1:3501606/148‑1 (MQ=255)

                                                                                                                                          cagtcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGgcg          <  1:3432160/148‑1 (MQ=255)

                                                                                                                                                ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    >  1:1364863/1‑148 (MQ=255)

                                                                                                                                                ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    <  1:1766436/148‑1 (MQ=255)

                                                                                                                                                ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    >  1:385636/1‑148 (MQ=255)

                                                                                                                                                ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTACTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    >  2:2750223/1‑148 (MQ=255)

CGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATT  >  CP009273/4611519‑4611812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`