Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,615,947 G→T 100% E73D (GAG→GAT)  radA → DNA repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,615,9470GT95.8% 638.9 / 16.2 214E73D (GAG→GAT) radADNA repair protein
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (103/102);  total (108/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

CAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCA  >  CP009273/4615803‑4616093
                                                                                                                                                |                                                                                                                                                  
   tgcCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCcg                                                                                                                                              >  2:3162215/1‑148 (MQ=255)
       tGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGtt                                                                                                                                          >  2:3356692/1‑148 (MQ=255)
        gTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGttt                                                                                                                                         >  2:1031621/1‑148 (MQ=255)
           aTGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTc                                                                                                                                      <  1:493390/148‑1 (MQ=255)
             gCCTGGAACACCATCACCGTGGTGCGTGTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCa                                                                                                                                    <  1:25733/148‑1 (MQ=255)
             gCCTGGAACACCATCACCGAGGGGCGTCTTGCTGCGTCGCCACTGGTGGCGCGTAACGAGCGTCTCCGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCa                                                                                                                                    <  1:25680/148‑1 (MQ=255)
                tGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCg                                                                                                                                 >  1:1113846/1‑148 (MQ=255)
                tGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCg                                                                                                                                 >  1:379117/1‑148 (MQ=255)
                tGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCg                                                                                                                                 >  1:381638/1‑148 (MQ=255)
                   aaCACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGAt                                                                                                                              <  2:1027381/148‑1 (MQ=255)
                   aaCACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGAt                                                                                                                              >  1:2809239/1‑148 (MQ=255)
                   aaCACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGAt                                                                                                                              >  1:2194846/1‑148 (MQ=255)
                    acacCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGAtt                                                                                                                             >  1:565537/1‑148 (MQ=255)
                     cacCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTCGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGAttt                                                                                                                            >  1:205539/1‑148 (MQ=255)
                     cacCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGAttt                                                                                                                            >  1:1293600/1‑148 (MQ=255)
                       ccATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTaa                                                                                                                          <  2:1211707/148‑1 (MQ=255)
                       ccATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTaa                                                                                                                          <  2:2413749/148‑1 (MQ=255)
                         aTCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAag                                                                                                                        >  1:295140/1‑148 (MQ=255)
                           cACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAagag                                                                                                                      >  1:413778/1‑148 (MQ=255)
                           cACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAagag                                                                                                                      <  2:2052130/148‑1 (MQ=255)
                           cACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAagag                                                                                                                      <  2:3138634/148‑1 (MQ=255)
                            aCCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGt                                                                                                                     <  2:1434473/148‑1 (MQ=255)
                             ccGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGtt                                                                                                                    <  1:1032618/148‑1 (MQ=255)
                             ccGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGtt                                                                                                                    <  1:175997/148‑1 (MQ=255)
                                 ggTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGAc                                                                                                                <  1:337393/148‑1 (MQ=255)
                                 ggTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGAc                                                                                                                >  2:1593088/1‑148 (MQ=255)
                                    gCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACcgc                                                                                                             >  2:487214/1‑148 (MQ=255)
                                          tgctgcGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTa                                                                                                       <  2:851228/148‑1 (MQ=255)
                                            ctgcGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAgg                                                                                                     <  1:3213386/148‑1 (MQ=255)
                                               cGTCGCCACTGGGGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAggcgg                                                                                                  <  1:779311/148‑1 (MQ=255)
                                               cGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAggcgg                                                                                                  <  1:2483703/148‑1 (MQ=255)
                                                 tCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAggcggcg                                                                                                >  2:1020548/1‑148 (MQ=255)
                                                  cGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAggcggcgg                                                                                               >  2:292630/1‑148 (MQ=255)
                                                       aTGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCgtgg                                                                                          >  2:900572/1‑148 (MQ=255)
                                                       aTGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCgtgg                                                                                          <  1:3554056/148‑1 (MQ=255)
                                                        tggtggCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCgtggt                                                                                         >  1:630439/1‑148 (MQ=255)
                                                          gtggCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGc                                                                                       <  2:2017843/148‑1 (MQ=255)
                                                             gcgcGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAg                                                                                    >  2:540478/1‑148 (MQ=255)
                                                             gcgcGTAACGAGCGTCTCAGCGGCTAGGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAg                                                                                    <  1:3289326/148‑1 (MQ=255)
                                                                   aaCGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTg                                                                              <  2:1299818/148‑1 (MQ=255)
                                                                   aaCGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTg                                                                              >  2:2482776/1‑148 (MQ=255)
                                                                    aCGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGc                                                                             >  2:2529026/1‑148 (MQ=255)
                                                                     cGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGACGTGcc                                                                            >  2:1805398/1‑148 (MQ=255)
                                                                     cGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGcc                                                                            <  1:2281790/148‑1 (MQ=255)
                                                                       aGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAt                                                                          >  2:222981/1‑148 (MQ=255)
                                                                       aGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAt                                                                          >  2:3046108/1‑148 (MQ=255)
                                                                        gagTCTCATCGGCTATGGCGGTAGCGCCGGTGTTGCAAAAGTCCAGAAACTCTCCGATATCTTGCTTGAAGATCTGCCGCGTTTTTCCACCGGTTTTTAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCATGAAGTTCCAtt                                                                         <  2:2646597/146‑1 (MQ=255)
                                                                        gCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAtt                                                                         >  1:2779220/1‑148 (MQ=255)
                                                                        gCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAtt                                                                         <  1:1962851/148‑1 (MQ=255)
                                                                         cGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTc                                                                        >  1:2528515/1‑148 (MQ=255)
                                                                            ctcAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAtt                                                                         >  2:1915683/1‑144 (MQ=255)
                                                                            ctcAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAtt                                                                         <  1:1915683/144‑1 (MQ=255)
                                                                              cAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGAtt                                                                   <  2:1700475/148‑1 (MQ=255)
                                                                               aGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTg                                                                  >  2:3205874/1‑148 (MQ=255)
                                                                                 cGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGc                                                                >  1:1892956/1‑148 (MQ=255)
                                                                                  ggCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCg                                                               <  2:234214/148‑1 (MQ=255)
                                                                                    cTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGt                                                             >  2:2285668/1‑148 (MQ=255)
                                                                                    cTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGt                                                             >  1:2862393/1‑148 (MQ=255)
                                                                                      aTGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTaa                                                           <  2:808452/148‑1 (MQ=255)
                                                                                       tGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAAc                                                          >  1:3436678/1‑148 (MQ=255)
                                                                                        gCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAAcc                                                         <  2:2194846/148‑1 (MQ=255)
                                                                                         ccGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAAccc                                                        >  2:920803/1‑148 (MQ=255)
                                                                                                cgcCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCg                                                 <  1:1845326/148‑1 (MQ=255)
                                                                                                 gcCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCgg                                                <  1:3267518/148‑1 (MQ=255)
                                                                                                 gcCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCgg                                                <  1:2851590/148‑1 (MQ=255)
                                                                                                 gcCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCgg                                                <  1:299083/148‑1 (MQ=255)
                                                                                                   cGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCgggg                                              <  2:1678847/148‑1 (MQ=255)
                                                                                                     gggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                            >  1:99617/1‑148 (MQ=255)
                                                                                                     gggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                            >  1:2243730/1‑148 (MQ=255)
                                                                                                     gggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                            <  2:3454886/148‑1 (MQ=255)
                                                                                                      ggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaaa                                           >  1:1212059/1‑148 (MQ=255)
                                                                                                        tGGCATAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGATTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCCTTCTGATTGGCGGTAACCCTGGTGCGGGGAAATc                                         <  2:2994724/148‑1 (MQ=255)
                                                                                                        tGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATc                                         <  2:1836319/148‑1 (MQ=255)
                                                                                                         ggCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATcc                                        <  2:205539/148‑1 (MQ=255)
                                                                                                             aaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgc                                    >  1:695143/1‑148 (MQ=255)
                                                                                                             aaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgc                                    >  1:695202/1‑148 (MQ=255)
                                                                                                                gTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgctgc                                 >  1:1739355/1‑148 (MQ=255)
                                                                                                                  ccAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAACTCCACGCTGCTa                               >  2:2792704/1‑148 (MQ=255)
                                                                                                                    ataaaCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGAACGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGGGGGGAAATCCACGCTGCTACt                             >  2:741820/3‑148 (MQ=255)
                                                                                                                     gAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTg                            >  1:178884/1‑148 (MQ=255)
                                                                                                                     gAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTg                            >  1:804822/1‑148 (MQ=255)
                                                                                                                     gAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTg                            >  2:467480/1‑148 (MQ=255)
                                                                                                                        aCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaa                         >  1:234088/1‑148 (MQ=255)
                                                                                                                        aCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaa                         >  1:234278/1‑148 (MQ=255)
                                                                                                                        aCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaa                         >  1:3096010/1‑148 (MQ=255)
                                                                                                                        aCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaa                         <  1:3356692/148‑1 (MQ=255)
                                                                                                                         ctctCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaaa                        >  1:263105/1‑148 (MQ=255)
                                                                                                                          tctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAAc                       >  2:3074099/1‑148 (MQ=255)
                                                                                                                           ctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGACATCCACGCTGCTACTGCAAACg                      >  2:1868929/1‑148 (MQ=255)
                                                                                                                           ctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACg                      <  1:1069111/148‑1 (MQ=255)
                                                                                                                             ccGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCt                    >  1:54814/1‑148 (MQ=255)
                                                                                                                              cGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtg                   <  2:1397065/148‑1 (MQ=255)
                                                                                                                              cGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtg                   <  1:3139296/148‑1 (MQ=255)
                                                                                                                                atatCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtgtg                 <  2:2797379/148‑1 (MQ=255)
                                                                                                                                atatCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtgtg                 <  2:1490461/148‑1 (MQ=255)
                                                                                                                                   tCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCaa              >  1:3078694/1‑148 (MQ=255)
                                                                                                                                       ccTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTg          >  1:704565/1‑148 (MQ=255)
                                                                                                                                       ccTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTg          >  1:1153879/1‑148 (MQ=255)
                                                                                                                                        cTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTgg         <  1:913242/148‑1 (MQ=255)
                                                                                                                                        cTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTgg         >  1:1344919/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCA  >  CP009273/4615803‑4616093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑