Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 434,663 A→C 100% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273434,6630AC100.0% 900.9 / ‑6.7 226I324S (ATC→AGC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (105/120);  total (105/121)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

GTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACT  >  CP009273/434518‑434807
                                                                                                                                                 |                                                                                                                                                
  gtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                              >  1:1278980/1‑148 (MQ=255)
  gtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                              <  1:635426/148‑1 (MQ=255)
       tCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAAt                                                                                                                                         <  1:1208620/148‑1 (MQ=255)
        cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATa                                                                                                                                        <  2:1451149/148‑1 (MQ=255)
         ggCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                       <  1:2175663/148‑1 (MQ=255)
         ggCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                       <  1:1500930/148‑1 (MQ=255)
            aaTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTc                                                                                                                                    >  1:756179/1‑148 (MQ=255)
              ttGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCgg                                                                                                                                  <  1:1668293/148‑1 (MQ=255)
                gCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCa                                                                                                                                <  2:840031/148‑1 (MQ=255)
                  cACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCaaa                                                                                                                              <  2:2280178/148‑1 (MQ=255)
                  cACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCaaa                                                                                                                              <  1:1268894/148‑1 (MQ=255)
                   aCGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAc                                                                                                                             <  2:2445177/148‑1 (MQ=255)
                    cGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCACAcc                                                                                                                            >  2:1548246/1‑148 (MQ=255)
                      tCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgc                                                                                                                          >  2:326374/1‑148 (MQ=255)
                       cGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgcc                                                                                                                         >  1:805717/1‑148 (MQ=255)
                       cGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGCTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgcc                                                                                                                         >  2:2779170/1‑148 (MQ=255)
                         aaGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACcgccgc                                                                                                                       <  2:2863704/148‑1 (MQ=255)
                         aaGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACcgccgc                                                                                                                       >  2:1231075/1‑148 (MQ=255)
                           gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTa                                                                                                                     >  2:2969230/1‑148 (MQ=255)
                           gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTa                                                                                                                     >  1:2972649/1‑148 (MQ=255)
                           gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTa                                                                                                                     >  1:2480759/1‑148 (MQ=255)
                            tAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTAc                                                                                                                    >  1:244200/1‑148 (MQ=255)
                                 aTCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTc                                                                                                               <  1:396518/148‑1 (MQ=255)
                                      ggAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCaa                                                                                                          >  1:2389109/1‑148 (MQ=255)
                                       gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCACCGCTACTTTTCGGCaaa                                                                                                         >  1:1572311/1‑148 (MQ=255)
                                         aTTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCCAACCGCCGCTACTTTTCGGCAaaca                                                                                                       >  1:900750/1‑148 (MQ=255)
                                         aTTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaaca                                                                                                       <  2:349362/148‑1 (MQ=255)
                                          tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaa                                                                                                      >  2:1121005/1‑148 (MQ=255)
                                          tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaa                                                                                                      >  2:445131/1‑148 (MQ=255)
                                             aCGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCg                                                                                                   <  2:2011644/148‑1 (MQ=255)
                                              cGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGc                                                                                                  <  1:3145010/148‑1 (MQ=255)
                                               gTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCt                                                                                                 >  1:1885681/1‑148 (MQ=255)
                                                    aaCTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAgg                                                                                            >  2:214684/1‑148 (MQ=255)
                                                     aCTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAggg                                                                                           <  1:2336439/148‑1 (MQ=255)
                                                      cTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGa                                                                                          >  1:3298529/1‑148 (MQ=255)
                                                      cTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGa                                                                                          <  2:2284129/148‑1 (MQ=255)
                                                           ccATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaaa                                                                                     >  2:2390539/1‑148 (MQ=255)
                                                             aTGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAtt                                                                                   >  2:1182416/1‑148 (MQ=255)
                                                              tGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAttt                                                                                  >  1:3125845/1‑148 (MQ=255)
                                                              tGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAttt                                                                                  >  1:2570898/1‑148 (MQ=255)
                                                               gCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTa                                                                                 <  1:550463/148‑1 (MQ=255)
                                                                   gcaCCTTCACGCATCGCCGGAGTAATCGCCATCCGCGTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCa                                                                             <  2:1888492/146‑1 (MQ=255)
                                                                   gAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCa                                                                             <  2:1852807/148‑1 (MQ=255)
                                                                     aCCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACg                                                                           <  2:3044711/148‑1 (MQ=255)
                                                                      ccTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACgg                                                                          >  2:2667545/1‑148 (MQ=255)
                                                                        ttCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCg                                                                        <  2:2736257/148‑1 (MQ=255)
                                                                        ttCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCg                                                                        <  1:3259893/148‑1 (MQ=255)
                                                                         tCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGt                                                                       >  1:3420655/1‑148 (MQ=255)
                                                                          cACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCCAACAACCGCTGGAGGGCTCAAATTTAGGCACGGCGTg                                                                      >  1:2696331/1‑148 (MQ=255)
                                                                                  gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGa                                                              <  2:37455/148‑1 (MQ=255)
                                                                                  gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGa                                                              <  2:36442/148‑1 (MQ=255)
                                                                                   ccGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGACAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGAt                                                             >  1:3428921/1‑148 (MQ=255)
                                                                                       aGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCggg                                                         >  2:1776313/1‑148 (MQ=255)
                                                                                       aGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCggg                                                         >  1:428325/1‑148 (MQ=255)
                                                                                        gTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGt                                                        <  2:1278980/148‑1 (MQ=255)
                                                                                         tAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                       >  2:851777/1‑148 (MQ=255)
                                                                                         tAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                       <  1:2115572/148‑1 (MQ=255)
                                                                                          aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCCAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                      >  1:1895838/1‑148 (MQ=255)
                                                                                          aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                      >  2:2340623/1‑148 (MQ=255)
                                                                                          aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                      >  2:104144/1‑148 (MQ=255)
                                                                                          aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                      <  1:2867601/148‑1 (MQ=255)
                                                                                          aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                      <  1:3087309/148‑1 (MQ=255)
                                                                                           aTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCtt                                                     >  1:2758257/1‑148 (MQ=255)
                                                                                            tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttt                                                    <  1:2318870/148‑1 (MQ=255)
                                                                                              gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttttt                                                  <  2:2978101/148‑1 (MQ=255)
                                                                                               ccATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTc                                                 <  1:55617/148‑1 (MQ=255)
                                                                                                cATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                >  2:88787/1‑148 (MQ=255)
                                                                                                  tCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGc                                              <  1:2176138/148‑1 (MQ=255)
                                                                                                    aGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCg                                            >  1:1823256/1‑148 (MQ=255)
                                                                                                           tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                     >  1:641868/1‑148 (MQ=255)
                                                                                                           tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                     <  2:2571927/148‑1 (MQ=255)
                                                                                                           tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                     <  2:783697/148‑1 (MQ=255)
                                                                                                            gtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATaa                                    <  1:84167/148‑1 (MQ=255)
                                                                                                                ttCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCAc                                >  2:1687186/1‑148 (MQ=255)
                                                                                                                  cGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGa                              <  2:1017186/148‑1 (MQ=255)
                                                                                                                  cGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGa                              <  1:1215300/148‑1 (MQ=255)
                                                                                                                    cTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGGAAACAACCGCTGGAGGGATCAAATTTATGGACGGCGTGGAAAGTGATCGGGTGTTTTTATTCCGGTTCATTAGCAAGAcc                            >  1:1039030/1‑148 (MQ=255)
                                                                                                                     tGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCt                           <  2:805717/148‑1 (MQ=255)
                                                                                                                      gCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtt                          >  1:2921219/1‑148 (MQ=255)
                                                                                                                      gCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtt                          >  1:2553071/1‑148 (MQ=255)
                                                                                                                          tttCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtttttt                      >  2:650500/1‑148 (MQ=255)
                                                                                                                           ttCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTg                     <  2:244127/148‑1 (MQ=255)
                                                                                                                               cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTACTGCCGGTTCATAACCACGACCTTTTTTGGTCa                 >  2:625815/1‑148 (MQ=255)
                                                                                                                                acaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCAt                <  1:1479370/148‑1 (MQ=255)
                                                                                                                                 caACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATg               <  2:1401001/148‑1 (MQ=255)
                                                                                                                                   aCCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGat             >  1:2474639/1‑148 (MQ=255)
                                                                                                                                    ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGata            <  1:1908574/148‑1 (MQ=255)
                                                                                                                                    ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGata            >  2:1084849/1‑148 (MQ=255)
                                                                                                                                     cAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat           >  2:2803334/1‑148 (MQ=255)
                                                                                                                                     cAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat           >  2:743053/1‑148 (MQ=255)
                                                                                                                                     cAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat           >  2:743376/1‑148 (MQ=255)
                                                                                                                                      aGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATg          <  2:50892/148‑1 (MQ=255)
                                                                                                                                       gTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGc         <  1:1366726/148‑1 (MQ=255)
                                                                                                                                       gTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGc         <  2:2480718/148‑1 (MQ=255)
                                                                                                                                       gTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGc         >  2:2314727/1‑148 (MQ=255)
                                                                                                                                        tCGCCAAAGCTTTTTTAATATCTCGGCAAACCGCCGCTACTTTTCGTCAAACAACCGCTTGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa        <  1:3389022/148‑1 (MQ=255)
                                                                                                                                         cGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAg       <  2:3372062/148‑1 (MQ=255)
                                                                                                                                         cGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAg       <  2:1606159/148‑1 (MQ=255)
                                                                                                                                          gCCAAAGCATTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAgg      <  2:3023115/148‑1 (MQ=255)
                                                                                                                                            cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGaa    >  1:1125703/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACT  >  CP009273/434518‑434807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑