Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 444,489 G→A 100% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,4890GA97.7% 576.6 / 2.7 177R537C (CGT→TGT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (85/88);  total (86/91)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

ATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGG  >  CP009273/444344‑444633
                                                                                                                                                 |                                                                                                                                                
atatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGc                                                                                                                                                <  2:790285/148‑1 (MQ=255)
  atGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCa                                                                                                                                              >  2:1478800/1‑148 (MQ=255)
   tGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAc                                                                                                                                             >  1:2837246/1‑148 (MQ=255)
         tcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACg                                                                                                                                       <  2:261369/148‑1 (MQ=255)
         tcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACg                                                                                                                                       <  2:261366/148‑1 (MQ=255)
           ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACggg                                                                                                                                     <  1:1029223/148‑1 (MQ=255)
           ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACggg                                                                                                                                     <  1:2810638/148‑1 (MQ=255)
               tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGc                                                                                                                                 >  2:1232271/1‑148 (MQ=255)
               tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGc                                                                                                                                 <  1:2997514/148‑1 (MQ=255)
               tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGc                                                                                                                                 <  1:2241393/148‑1 (MQ=255)
                aGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGcc                                                                                                                                >  1:1900117/1‑148 (MQ=255)
                 gTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCa                                                                                                                               >  2:1040774/1‑148 (MQ=255)
                 gTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCa                                                                                                                               <  2:161665/148‑1 (MQ=255)
                  tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAg                                                                                                                              >  1:2576056/1‑148 (MQ=255)
                  tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAg                                                                                                                              >  1:2576228/1‑148 (MQ=255)
                   ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGt                                                                                                                             >  1:2075969/1‑148 (MQ=255)
                   ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGt                                                                                                                             <  1:120498/148‑1 (MQ=255)
                    gTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                            <  2:1374969/148‑1 (MQ=255)
                    gTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                            <  2:1374976/148‑1 (MQ=255)
                      cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                          >  1:3335178/1‑148 (MQ=255)
                      cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                          <  1:703150/148‑1 (MQ=255)
                      cAGGCTTTTTATACGCTTCGCATTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCAAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                          >  2:2314314/1‑148 (MQ=255)
                                ataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                                                                                >  1:1195692/1‑148 (MQ=255)
                                ataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                                                                                <  1:1628931/148‑1 (MQ=255)
                                 taCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCt                                                                                                               <  1:801389/148‑1 (MQ=255)
                                  aCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTg                                                                                                              >  2:3140376/1‑148 (MQ=255)
                                  aCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTg                                                                                                              >  2:34344/1‑148 (MQ=255)
                                    gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggt                                                                                                            >  1:2139995/1‑148 (MQ=255)
                                    gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggg                                                                                                            >  1:2064823/1‑147 (MQ=255)
                                       tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                         >  2:3073227/1‑148 (MQ=255)
                                       tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                         >  2:2058399/1‑148 (MQ=255)
                                       tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                         >  2:806683/1‑148 (MQ=255)
                                        cGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcg                                                                                                        <  2:2999472/148‑1 (MQ=255)
                                        cGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcg                                                                                                        <  1:653807/148‑1 (MQ=255)
                                           cTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                     >  1:2893862/1‑148 (MQ=255)
                                           cTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                     >  1:1420843/1‑148 (MQ=255)
                                            tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcg                                                                                                    >  2:102984/1‑148 (MQ=255)
                                             ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                   >  1:11423/1‑148 (MQ=255)
                                             ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                   >  1:946990/1‑148 (MQ=255)
                                             ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                   >  2:106394/1‑148 (MQ=255)
                                              tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCATCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                                  >  1:1288639/1‑148 (MQ=255)
                                                tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                                <  1:3005323/148‑1 (MQ=255)
                                                tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                                <  1:1110609/148‑1 (MQ=255)
                                                tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                                <  1:3005374/148‑1 (MQ=255)
                                                tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                                >  1:1928638/1‑148 (MQ=255)
                                                 ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                               >  1:2563429/1‑148 (MQ=255)
                                                 ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                               >  2:2284354/1‑148 (MQ=255)
                                                 ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                               >  2:490054/1‑148 (MQ=255)
                                                     cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaa                                                                                           <  2:2478672/148‑1 (MQ=255)
                                                      aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaaa                                                                                          <  2:3368463/148‑1 (MQ=255)
                                                      aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaaa                                                                                          <  1:236027/148‑1 (MQ=255)
                                                       tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                         >  2:3405711/1‑148 (MQ=255)
                                                       tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                         >  1:667198/1‑148 (MQ=255)
                                                        ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                                                                                        <  2:1816141/148‑1 (MQ=255)
                                                            cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                       >  2:2971122/1‑145 (MQ=255)
                                                            cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                       <  1:2972469/145‑1 (MQ=255)
                                                            cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                       >  2:2972469/1‑145 (MQ=255)
                                                             aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                   <  2:663828/148‑1 (MQ=255)
                                                             aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                   <  2:663852/148‑1 (MQ=255)
                                                             aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                   <  2:2866955/148‑1 (MQ=255)
                                                             aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTACAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                   <  2:618987/148‑1 (MQ=255)
                                                                aTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTg                                                                                >  1:2111494/1‑148 (MQ=255)
                                                                  gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGaa                                                                              <  1:1778227/148‑1 (MQ=255)
                                                                  gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGaa                                                                              <  1:2459528/148‑1 (MQ=255)
                                                                     tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa                                                                           <  2:1455752/148‑1 (MQ=255)
                                                                     tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa                                                                           >  1:3515260/1‑148 (MQ=255)
                                                                     tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa                                                                           >  1:3514834/1‑148 (MQ=255)
                                                                         gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAg                                                                       <  2:1397844/148‑1 (MQ=255)
                                                                           tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                     >  2:650091/1‑148 (MQ=255)
                                                                                  cGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                              >  2:196433/1‑148 (MQ=255)
                                                                                     gCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATAcc                                                           <  2:3523767/148‑1 (MQ=255)
                                                                                           cTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcgc                                                     >  2:3327089/1‑148 (MQ=255)
                                                                                           cTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcgc                                                     >  2:1559623/1‑148 (MQ=255)
                                                                                                gCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGTTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCa                                                <  2:674161/148‑1 (MQ=255)
                                                                                                  aaaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                              <  1:2777438/148‑1 (MQ=255)
                                                                                                        ataGAACGGAGGCGGGGTAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGAAGAGAATACCCAGCGCAATCAGTACTGCa                                        <  1:3277236/148‑1 (MQ=255)
                                                                                                        ataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCa                                        <  2:1766429/148‑1 (MQ=255)
                                                                                                          aGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAcc                                      >  2:651901/1‑148 (MQ=255)
                                                                                                             aCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgct                                   >  2:1570768/1‑148 (MQ=255)
                                                                                                             aCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgct                                   >  2:2739324/1‑148 (MQ=255)
                                                                                                                   gCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGc                             <  1:1040774/148‑1 (MQ=255)
                                                                                                                    cGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCa                            >  2:1754735/1‑148 (MQ=255)
                                                                                                                       ggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatc                         <  2:2796059/148‑1 (MQ=255)
                                                                                                                         aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                       <  1:1275760/148‑1 (MQ=255)
                                                                                                                           gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                     >  1:3121691/1‑148 (MQ=255)
                                                                                                                           gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                     <  1:1374748/148‑1 (MQ=255)
                                                                                                                           gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                     <  1:1374746/148‑1 (MQ=255)
                                                                                                                              gTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa                  >  2:3544321/1‑148 (MQ=255)
                                                                                                                              gTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa                  <  1:1714947/148‑1 (MQ=255)
                                                                                                                               ttGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                 >  2:2563459/1‑148 (MQ=255)
                                                                                                                               ttGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                 >  2:1442643/1‑148 (MQ=255)
                                                                                                                               ttGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                 <  1:2284354/148‑1 (MQ=255)
                                                                                                                               ttGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                 <  1:490054/148‑1 (MQ=255)
                                                                                                                                 gCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATgg               <  1:1737221/148‑1 (MQ=255)
                                                                                                                                 gCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATgg               <  2:1928082/148‑1 (MQ=255)
                                                                                                                                  cccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt              <  2:2559795/148‑1 (MQ=255)
                                                                                                                                  cccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt              >  2:591067/1‑148 (MQ=255)
                                                                                                                                         cAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAAc       <  2:3424939/148‑1 (MQ=255)
                                                                                                                                            cGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGc    >  2:2292992/1‑148 (MQ=255)
                                                                                                                                             gTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCg   <  2:2075969/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGG  >  CP009273/444344‑444633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑