Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 679.5 / NA 205intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (98/107);  total (98/107)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 206 total aligned reads displayed.

ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450145‑450434
                                                                                                                                                 |                                                                                                                                                    
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   >  1:450225/1‑148 (MQ=255)
  gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                                  >  1:2655234/1‑148 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                 <  1:1034257/148‑1 (MQ=255)
      tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATg                                                                                                                                              >  2:877436/1‑148 (MQ=255)
           ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  2:2807377/148‑1 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  1:73572/1‑148 (MQ=255)
                     acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                               >  2:69341/1‑148 (MQ=255)
                      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGAAACTGTCGGCGATTTTCAAGTATGTTGATAAAAATTTTATCAAAAACGGCCTGCggg                                                                                                                              <  1:1221209/148‑1 (MQ=255)
                      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCggg                                                                                                                              >  2:345662/1‑148 (MQ=255)
                       acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                             <  2:2276433/148‑1 (MQ=255)
                        cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                            >  2:1652989/1‑148 (MQ=255)
                           ttAAGGAGTGGGACGGGTGGCAGGCCACCGTCTTGGCCCCCCCGCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         <  1:1907863/148‑1 (MQ=255)
                           ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  1:3537178/1‑148 (MQ=255)
                           ttAAGGAGTGGGAAGGGTAGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  2:2630758/1‑148 (MQ=255)
                              aGGAGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                      <  1:1618245/148‑1 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑t                                                                                                                    <  2:2620310/148‑1 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑t                                                                                                                    <  1:3540415/148‑1 (MQ=255)
                                  gTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tttt                                                                                                                 <  2:1899807/148‑1 (MQ=255)
                                  gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tttt                                                                                                                 <  2:1103042/148‑1 (MQ=255)
                                     ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑ttttttg                                                                                                              >  1:1450983/1‑145 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTTTCt                                                                                                             >  2:420809/1‑148 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGggtg‑ttttgtgt                                                                                                             >  1:3441365/1‑146 (MQ=255)
                                         gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTG‑TTTTGTGTggg                                                                                                          >  1:26948/1‑147 (MQ=255)
                                           gTTGCAGCACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑Att                                                                                                       <  1:3354128/148‑1 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑Attt                                                                                                      >  2:2245524/1‑148 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGGTG‑TTTTTTGTggtgtt                                                                                                       >  1:1662890/1‑144 (MQ=255)
                                              gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTg                                                                                                    <  2:1151206/148‑1 (MQ=255)
                                               cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTGTCTGG‑ATTTTgc                                                                                                   >  2:3171870/1‑148 (MQ=255)
                                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTgcgc                                                                                                 <  1:685142/148‑1 (MQ=255)
                                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTgcgc                                                                                                 <  2:1562607/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑tt                                                                                              <  2:958935/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTTTGTTG‑TTTTTGGGC‑tt                                                                                              >  1:2245993/1‑148 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑ttt                                                                                             <  1:1745894/148‑1 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑ttt                                                                                             <  1:1714874/148‑1 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTTTGTGG‑CTTTTtcgggtt                                                                                              >  2:2991525/1‑144 (MQ=255)
                                                     acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑tttt                                                                                            <  1:2337251/148‑1 (MQ=255)
                                                     acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTGTGTGG‑GTTTTgggggttt                                                                                             >  2:1438/1‑141 (MQ=255)
                                                         tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGcc                                                                                        <  1:2364287/148‑1 (MQ=255)
                                                            ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTGG‑GTTTGTGTGG‑TTTTTGCGC‑TTTTTGaccct                                                                                     >  2:799636/1‑146 (MQ=255)
                                                             tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGc                                                                                    <  1:2971860/148‑1 (MQ=255)
                                                               ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTGTGG‑ATTTTTCGC‑TTTTTGCCCCGCAt                                                                                  >  2:1697761/1‑148 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCAtt                                                                                 <  2:785804/148‑1 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCAtt                                                                                 <  1:1538881/148‑1 (MQ=255)
                                                                  ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGTCCAGCATTCa                                                                               >  2:3274018/1‑148 (MQ=255)
                                                                   tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAg                                                                              <  2:3443573/148‑1 (MQ=255)
                                                                    ccccccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAAGTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAGGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGa                                                                             <  1:929649/145‑1 (MQ=255)
                                                                      tccCTGTCGTGTATCATGAAGCATAGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTACCAGTATGTTGCTAAAATTTTTTTGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACg                                                                           <  1:1404803/148‑1 (MQ=255)
                                                                      tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACg                                                                           <  2:190592/148‑1 (MQ=255)
                                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑a                                                                         <  1:3054546/148‑1 (MQ=255)
                                                                        ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTGTGG‑ATTTTGCGC‑TGTTTGCCCAGCATTCAGACG‑aa                                                                        >  2:2444407/1‑148 (MQ=255)
                                                                         cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGG‑ATTTTGCGC‑TTTTTTCCCAGCCTTAAGACG‑aaa                                                                       >  2:2787829/1‑148 (MQ=255)
                                                                          tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCAATTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGG‑GTTTTTGGG‑TTTTTTGCCACCATTAAGAAG‑caaa                                                                      >  1:525706/1‑148 (MQ=255)
                                                                           gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAAt                                                                     <  1:888453/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  2:1590798/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  1:3302491/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAACCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAACCGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  2:3359952/148‑1 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTGG‑TTTTTTGGCGTTTTTGCCC‑GCAATCATAAG‑AAAAATTCCCg                                                               >  1:2551031/1‑148 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg                                                               <  2:2100364/148‑1 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTTTTGT‑TTTTTGGGG‑TTTTTGGCC‑GCAATGAGACGAAAAATTGccg                                                                >  1:784896/1‑147 (MQ=255)
                                                                                  aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCgg                                                              >  2:290510/1‑148 (MQ=255)
                                                                                  aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTG‑TTTTTTTTTG‑GTTTTTGGC‑TTTTTTGCCAGCCATCAGACGAAAAATTGccgg                                                               >  1:229861/1‑146 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:3437209/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:902279/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:2857262/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTTTGGTTTTTTGGGT‑TTTTTGGCCGCCATAAGAACA‑AAAATTACCCggg                                                             >  1:700019/1‑148 (MQ=255)
                                                                                       gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTGG‑AGTTTTGGC‑TTTTTTGCCAGCCTTCAGACG‑AAAAATTCCCGGGGAtt                                                         >  1:281868/1‑148 (MQ=255)
                                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtg                                                        <  2:580152/148‑1 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtgtg                                                      <  1:710141/148‑1 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGG‑TTTTTGCGC‑TTTTTGCCCAGCATTAAGACG‑AAAATTGCCCCGGCAAtgtg                                                      >  2:2129111/1‑148 (MQ=255)
                                                                                              cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa                                                  <  2:1839502/148‑1 (MQ=255)
                                                                                              cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa                                                  <  2:34140/148‑1 (MQ=255)
                                                                                                cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TGTTGTTGGG‑CTTTTTGGC‑TTTTTTCCCCGCCTACAGACC‑AAAAAAGCCCCGGCATTTTTaaaaaa                                                >  2:1265366/1‑148 (MQ=255)
                                                                                                  tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGG‑TTTTTTCCCCGCATTCAGACG‑AAAAGTGCCCGGGAAGTGTGAAAAAATa                                              >  2:1322670/1‑148 (MQ=255)
                                                                                                   aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAc                                             <  2:1934426/148‑1 (MQ=255)
                                                                                                     aatcGCATTTGAAACTGTCGGCGGTTTTCCAGTATTTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                           <  1:3022702/145‑1 (MQ=255)
                                                                                                     aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGG‑ATTTTTGGG‑TTTTTTCCCCGCATTGAGCCG‑AAAAATTCCCGGGCAGTGTGTAAAAATAagc                                           >  2:3673/1‑148 (MQ=255)
                                                                                                     aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGG‑ATTTTGCGC‑CTTTTGCCCAGCATTCAGACG‑AAAATTTCCCGGGAATTTTGAAAAAATAcgc                                           >  2:1120989/1‑148 (MQ=255)
                                                                                                              tGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                  <  1:242113/148‑1 (MQ=255)
                                                                                                                  aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGG‑ATTTTTCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGTAAAAATAAGCGACCGCGCCCAAt                              >  2:1075677/1‑148 (MQ=255)
                                                                                                                  aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTATGG‑ATTTTGCGG‑TTTTTGCCCAGCATTACGACG‑AAAATTGCCCGGGAAGTGTGGAAAAAAAAGCGACACCGCCCaaa                              >  2:3444092/1‑147 (MQ=255)
                                                                                                                   cggTCGGCGATTTTCCTGTTTGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                             <  1:1032693/146‑1 (MQ=255)
                                                                                                                    tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                            <  2:166566/148‑1 (MQ=255)
                                                                                                                       gggCGATTTCCCTGTATGTTCCAAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTGGCGC‑TTTTTGCCCAGCATTCAGACG‑AAATTTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCg                         <  1:2115218/147‑1 (MQ=255)
                                                                                                                       cGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTATGG‑ATTTTTCGC‑TTTTTTCCCACCAATCAGACG‑AAAAATGCCCGGGCATTTTTAAAAAATAACCGACAACGCGCAAAAACCg                         >  2:1840757/1‑148 (MQ=255)
                                                                                                                        ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                        <  2:2316959/148‑1 (MQ=255)
                                                                                                                         gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                       <  2:3537178/148‑1 (MQ=255)
                                                                                                                         gcgaTTTTCCAGTAGTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                       <  1:2603371/148‑1 (MQ=255)
                                                                                                                          cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGG‑ATTTTTCGC‑TTTTTTCCCAGCATTAAGACG‑AAAATTGCCCCGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGtta                      >  2:3477476/1‑147 (MQ=255)
                                                                                                                          cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCCGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCCtta                      >  2:2582301/1‑147 (MQ=255)
                                                                                                                             ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                   <  2:3155280/148‑1 (MQ=255)
                                                                                                                             ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTG‑TTTTGTGTGG‑ATTTTTCGG‑TTTTTTCCCAGCAATAAGACG‑AAAATTTCCCGGGAAATTTGAAAAAAAAAGCGACAACGCGCAAAAAACCTTCTCg                   >  1:2960312/1‑148 (MQ=255)
                                                                                                                                     tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTTGGC‑TTTTTGCCCAGCAATGAGACG‑AAAAATTCCCGGGAAGTGTTTAAAAATAAACGACACCGCGCAAAAAACGATCTCGACACATaa           >  2:704296/1‑148 (MQ=255)
                                                                                                                                      aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTTG‑ATTTTGCGC‑TTTTTTCCCAGCAATAAGACG‑AAAATTGCCCGGGCATTGTGAAAAAATAAGCGACAGCGCGCAAAAAACCTTCTCGACTCATaaa          >  2:2482560/1‑148 (MQ=255)
                                                                                                                                       tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTTCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa         >  2:2051748/1‑148 (MQ=255)
                                                                                                                                       tGTTGCAAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa         <  1:112686/148‑1 (MQ=255)
                                                                                                                                         ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt       <  2:2707973/148‑1 (MQ=255)
                                                                                                                                         ttGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt       <  1:3085478/148‑1 (MQ=255)
                                                                                                                                          tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTCTGG‑GTTTTTGGC‑TTTTTTGCCACCATACAGACG‑AAAAATTCCCGGGCATTGTTTAAAAAAAATCCACACCGCGCCAAAAACCTTCGCGACTCATAAAATTg      >  2:921951/1‑148 (MQ=255)
                                                                                                                                            cTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt    <  1:3401906/148‑1 (MQ=255)
                                                                                                                                              aaaaATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc  >  2:1493400/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450145‑450434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑