Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,298 T→A 100% L652Q (CTG→CAG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,2980TA100.0% 634.8 / NA 193L652Q (CTG→CAG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (80/113);  total (80/113)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

ACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456153‑456442
                                                                                                                                                 |                                                                                                                                                
 cGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAggg                                                                                                                                               <  2:2550009/148‑1 (MQ=255)
 cGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAggg                                                                                                                                               <  2:2550296/148‑1 (MQ=255)
  ggAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgggg                                                                                                                                              <  2:1074826/148‑1 (MQ=255)
  ggAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgggg                                                                                                                                              <  2:1073952/148‑1 (MQ=255)
    aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGAt                                                                                                                                            >  2:799552/1‑148 (MQ=255)
        gggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAc                                                                                                                                        <  2:1612084/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   <  2:2668856/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   <  2:3077010/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   >  2:299965/1‑148 (MQ=255)
              tGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAt                                                                                                                                  >  2:1959618/1‑148 (MQ=255)
              tGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAt                                                                                                                                  >  2:2783754/1‑148 (MQ=255)
               gACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAtt                                                                                                                                 >  2:3119081/1‑148 (MQ=255)
                aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttt                                                                                                                                <  1:611984/148‑1 (MQ=255)
                aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttt                                                                                                                                <  2:2326749/148‑1 (MQ=255)
                aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttt                                                                                                                                <  2:2406577/148‑1 (MQ=255)
                aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttt                                                                                                                                <  2:2406978/148‑1 (MQ=255)
                   tGCTGACCTTTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                             <  2:1137168/148‑1 (MQ=255)
                   tGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                             <  2:3488991/148‑1 (MQ=255)
                   tGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                             <  2:797493/148‑1 (MQ=255)
                   tGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                             <  2:3248465/148‑1 (MQ=255)
                   tGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                             >  1:2094972/1‑148 (MQ=255)
                        aCCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaa                                                                                                                        <  1:1937603/148‑1 (MQ=255)
                         ccATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaaa                                                                                                                       >  2:2828488/1‑148 (MQ=255)
                           aTTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAAc                                                                                                                     <  1:948964/148‑1 (MQ=255)
                            ttGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACg                                                                                                                    <  1:3237656/148‑1 (MQ=255)
                            ttGAAACCGCATGTGGTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACg                                                                                                                    <  1:3003662/148‑1 (MQ=255)
                                 aCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                               >  1:3435285/1‑148 (MQ=255)
                                  ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              <  1:3130117/148‑1 (MQ=255)
                                  ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              >  2:603750/1‑148 (MQ=255)
                                           ttCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtcca                                                                                                     <  2:1680561/148‑1 (MQ=255)
                                            tCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccac                                                                                                    <  2:2188828/148‑1 (MQ=255)
                                             ccGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccacg                                                                                                   >  1:1474002/1‑148 (MQ=255)
                                             ccGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccacg                                                                                                   <  2:2005149/148‑1 (MQ=255)
                                               gggTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTa                                                                                                 <  2:872196/148‑1 (MQ=255)
                                                 gTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc                                                                                               >  1:3102297/1‑148 (MQ=255)
                                                  tAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCg                                                                                              >  2:388861/1‑148 (MQ=255)
                                                  tAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCg                                                                                              <  1:3518907/148‑1 (MQ=255)
                                                  tAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCg                                                                                              >  2:3306862/1‑148 (MQ=255)
                                                   aaaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCgg                                                                                             >  2:3238026/1‑148 (MQ=255)
                                                     aGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGaa                                                                                           >  2:1993050/1‑148 (MQ=255)
                                                       gCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg                                                                                         <  2:435087/148‑1 (MQ=255)
                                                        cAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGt                                                                                        >  1:2446062/1‑148 (MQ=255)
                                                           aCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     >  2:2943845/1‑148 (MQ=255)
                                                            cTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGa                                                                                    >  1:3564415/1‑148 (MQ=255)
                                                              gACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACg                                                                                  <  2:1424436/148‑1 (MQ=255)
                                                                ccTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                >  1:280508/1‑148 (MQ=255)
                                                                 cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCg                                                                               <  2:240890/148‑1 (MQ=255)
                                                                    taCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGaaa                                                                            >  2:3328890/1‑148 (MQ=255)
                                                                     aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGACGGTGCGACGCCGAAAg                                                                           >  2:2003070/1‑148 (MQ=255)
                                                                     aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                           <  1:100056/148‑1 (MQ=255)
                                                                     aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                           <  1:100053/148‑1 (MQ=255)
                                                                     aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                           <  1:2019298/148‑1 (MQ=255)
                                                                      ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGa                                                                          <  2:652387/148‑1 (MQ=255)
                                                                      ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGa                                                                          <  2:2391823/148‑1 (MQ=255)
                                                                       cGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGAt                                                                         >  1:1761307/1‑148 (MQ=255)
                                                                       cGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGAt                                                                         >  1:3105490/1‑148 (MQ=255)
                                                                        ggTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATg                                                                        <  1:1027377/148‑1 (MQ=255)
                                                                          ttCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      >  1:2621989/1‑148 (MQ=255)
                                                                           tCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTc                                                                     <  1:2319650/148‑1 (MQ=255)
                                                                           tCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTc                                                                     <  2:2407320/148‑1 (MQ=255)
                                                                             gCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCg                                                                   <  1:254371/148‑1 (MQ=255)
                                                                               tCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAg                                                                 <  2:3038492/148‑1 (MQ=255)
                                                                               tCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGGTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCCCGTACCGGAAGGTGCGCCGCCGAAAGATGGTCCGAg                                                                 <  1:701067/148‑1 (MQ=255)
                                                                                    gAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCg                                                            <  1:1099431/148‑1 (MQ=255)
                                                                                      aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGt                                                          >  2:1068015/1‑148 (MQ=255)
                                                                                       gTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTa                                                         <  1:1837699/148‑1 (MQ=255)
                                                                                       gTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTa                                                         <  1:107439/148‑1 (MQ=255)
                                                                                       gTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTa                                                         <  2:1674675/148‑1 (MQ=255)
                                                                                        tGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                        >  1:263007/1‑148 (MQ=255)
                                                                                                 aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               >  2:1308732/1‑148 (MQ=255)
                                                                                                 aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               >  1:2585981/1‑148 (MQ=255)
                                                                                                   tgcATTCAGGCTGCGTTTTCGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAATATCGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                             <  2:2125846/146‑1 (MQ=255)
                                                                                                    ccATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTCTTGCTATGTGCAc                                            >  2:1869059/1‑148 (MQ=255)
                                                                                                         cAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCgcgc                                       <  2:1017840/148‑1 (MQ=255)
                                                                                                         cAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCgcgc                                       <  2:3338019/148‑1 (MQ=255)
                                                                                                           ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTg                                     <  2:2554186/148‑1 (MQ=255)
                                                                                                           ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTg                                     <  1:2193943/148‑1 (MQ=255)
                                                                                                            gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                    >  2:526673/1‑148 (MQ=255)
                                                                                                            gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                    <  2:2111023/148‑1 (MQ=255)
                                                                                                            gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                    >  1:1605346/1‑148 (MQ=255)
                                                                                                             cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGt                                   <  2:1160699/148‑1 (MQ=255)
                                                                                                             cggcgTTAACGGGGGGGCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGt                                   <  1:273808/148‑1 (MQ=255)
                                                                                                               gcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGttt                                 >  1:1350387/1‑148 (MQ=255)
                                                                                                                cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                <  1:3447086/148‑1 (MQ=255)
                                                                                                                cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                <  1:1008915/148‑1 (MQ=255)
                                                                                                                 gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCt                               <  2:1768842/148‑1 (MQ=255)
                                                                                                                 gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCt                               <  2:2230899/148‑1 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:3397735/1‑148 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:175592/1‑148 (MQ=255)
                                                                                                                         tggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATACACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTGGCCTGAc                       >  1:1359752/1‑148 (MQ=255)
                                                                                                                          ggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGAcc                      >  2:2264381/1‑148 (MQ=255)
                                                                                                                           gtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCg                     >  1:2958133/1‑148 (MQ=255)
                                                                                                                           gtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCg                     >  1:1180053/1‑148 (MQ=255)
                                                                                                                             tCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGt                   >  1:3341732/1‑148 (MQ=255)
                                                                                                                               gtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTaa                 <  2:1484723/148‑1 (MQ=255)
                                                                                                                               gtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTaa                 <  2:3255846/148‑1 (MQ=255)
                                                                                                                               gtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTaa                 >  1:444756/1‑148 (MQ=255)
                                                                                                                                        cgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCg        <  2:2043913/148‑1 (MQ=255)
                                                                                                                                           aaaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGc     <  2:3213365/148‑1 (MQ=255)
                                                                                                                                              aaCAGGTGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGa  <  2:2927449/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456153‑456442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑