Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,499 G→T 100% R424S (CGC→AGC)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,4990GT98.0% 756.8 / 5.8 244R424S (CGC→AGC) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (136/103);  total (137/107)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461354‑461643
                                                                                                                                                 |                                                                                                                                                
agagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCttt                                                                                                                                                <  2:1394099/148‑1 (MQ=255)
agagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCttt                                                                                                                                                <  1:1306390/148‑1 (MQ=255)
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTAACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                               >  1:1513247/1‑148 (MQ=255)
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATAATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCTGTGTCGCCTGTAGGCGTTc                                                                                                                                               <  1:1179498/148‑1 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                              >  2:1332278/1‑148 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCATACGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                              >  1:655171/1‑148 (MQ=255)
   gTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTg                                                                                                                                             >  2:1558571/1‑148 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          <  1:1272444/148‑1 (MQ=255)
        ttCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATg                                                                                                                                        >  2:529393/1‑148 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGcc                                                                                                                                           >  2:686490/1‑144 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATgg                                                                                                                                       <  2:1860324/148‑1 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATgg                                                                                                                                       <  2:55283/148‑1 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATgg                                                                                                                                       >  1:1725462/1‑148 (MQ=255)
                    gCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCt                                                                                                                            <  1:1884195/148‑1 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           >  1:416203/1‑148 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           >  2:352694/1‑148 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           <  2:2561529/148‑1 (MQ=255)
                        aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                        >  1:2638115/1‑148 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                       >  1:551176/1‑148 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                       >  1:2955798/1‑148 (MQ=255)
                           aaTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                     >  2:2282034/1‑148 (MQ=255)
                           aaTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                     <  1:3088911/148‑1 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAg                                                                                                                    >  1:1002081/1‑148 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAg                                                                                                                    >  1:3156421/1‑148 (MQ=255)
                             tCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                                                                                                   <  2:252165/148‑1 (MQ=255)
                                gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGa                                                                                                                <  2:2425527/148‑1 (MQ=255)
                                       atcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCa                                                                                                         >  1:2311521/1‑148 (MQ=255)
                                        tcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAg                                                                                                        >  2:88324/1‑148 (MQ=255)
                                        tcatGAGGTCTGCGTGTGCATGAAGGCTCGTGTCGTCCCACTTTTTTGCATTATGAAAAATAATTGTGAGTTCACACCCTTCCGCTGACAGTGTCGCCTGTATGCTTTATGCCATGGTATTAAGTTCTATCGGTATGTGATAAACCAg                                                                                                        >  2:1639949/1‑148 (MQ=255)
                                          atGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCg                                                                                                      <  1:412422/148‑1 (MQ=255)
                                             aGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCCTGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCa                                                                                                   >  1:1246215/1‑148 (MQ=255)
                                             aGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCa                                                                                                   >  1:2110850/1‑148 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAgg                                                                                                >  2:2004534/1‑148 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAgg                                                                                                >  2:2691966/1‑148 (MQ=255)
                                                   gCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGttt                                                                                             <  1:3148294/148‑1 (MQ=255)
                                                   gCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGttt                                                                                             <  1:3148371/148‑1 (MQ=255)
                                                    cGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGtttt                                                                                            >  1:730003/1‑148 (MQ=255)
                                                         gCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTa                                                                                       <  2:653967/148‑1 (MQ=255)
                                                          ccTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAg                                                                                      <  2:516369/148‑1 (MQ=255)
                                                          ccTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAg                                                                                      <  2:1104198/148‑1 (MQ=255)
                                                           cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                     <  2:1766167/148‑1 (MQ=255)
                                                             gTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTAGGTAGttt                                                                                   >  1:2833503/1‑148 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTATTAAAAATAAATTTGAGTTAACATCCTTACGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTATATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAc                                                                                >  1:30257/1‑148 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAc                                                                                >  2:2437536/1‑148 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAc                                                                                >  1:1803441/1‑148 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAc                                                                                >  2:45344/1‑148 (MQ=255)
                                                                 ggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACt                                                                               >  2:629010/1‑148 (MQ=255)
                                                                   tcgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTc                                                                             <  1:1828159/148‑1 (MQ=255)
                                                                    cgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCa                                                                            <  1:2929542/148‑1 (MQ=255)
                                                                      tgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATc                                                                          <  2:1264817/148‑1 (MQ=255)
                                                                      agtagacccAGTTTTTTGCTTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTATATCGGTAGTTGATAAACAAGCGTCAATGATTTCGGTAGTTTGACTTCATc                                                                          >  2:3238574/7‑148 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    >  2:189478/1‑148 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    >  2:1001705/1‑148 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    <  1:917318/148‑1 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    <  1:2030835/148‑1 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    <  1:65362/148‑1 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    <  1:529393/148‑1 (MQ=255)
                                                                             ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                   >  2:2183940/1‑148 (MQ=255)
                                                                                 ttttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                               >  1:2792031/1‑148 (MQ=255)
                                                                                 ttttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                               <  2:3016281/148‑1 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                              >  1:2317534/1‑148 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAACCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                              >  2:662528/1‑148 (MQ=255)
                                                                                       gCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCg                                                         <  1:1604271/148‑1 (MQ=255)
                                                                                        cGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCgg                                                        >  2:2870786/1‑148 (MQ=255)
                                                                                        cGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCgg                                                        >  2:2868235/1‑148 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                      >  1:1014793/1‑148 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                      >  2:789085/1‑148 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                      <  2:2943602/148‑1 (MQ=255)
                                                                                            aTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATa                                                    >  1:1418978/1‑148 (MQ=255)
                                                                                            aTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATa                                                    >  1:2173195/1‑148 (MQ=255)
                                                                                            aTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATa                                                    >  2:3264636/1‑148 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                  >  2:2488281/1‑148 (MQ=255)
                                                                                               aaaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTc                                                 >  1:2853787/1‑148 (MQ=255)
                                                                                                aaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                                >  2:2636473/1‑148 (MQ=255)
                                                                                                aaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                                <  2:1832164/148‑1 (MQ=255)
                                                                                                      atTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTg                                          >  1:1152757/1‑148 (MQ=255)
                                                                                                        tgtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGc                                        <  2:2110850/148‑1 (MQ=255)
                                                                                                        tgtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGc                                        >  2:1966568/1‑148 (MQ=255)
                                                                                                         gtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCa                                       <  1:695598/148‑1 (MQ=255)
                                                                                                           gAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGt                                     >  2:912756/1‑148 (MQ=255)
                                                                                                               tCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATg                                 >  1:1226428/1‑148 (MQ=255)
                                                                                                                cacaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                <  1:3376869/148‑1 (MQ=255)
                                                                                                                  caGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCAt                              >  2:1207931/1‑148 (MQ=255)
                                                                                                                    gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGa                            <  1:1849419/148‑1 (MQ=255)
                                                                                                                     ccTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                           <  2:2746889/148‑1 (MQ=255)
                                                                                                                     ccTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                           <  1:88324/148‑1 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                        <  2:623640/148‑1 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                        >  1:2459450/1‑148 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                        >  2:1491522/1‑148 (MQ=255)
                                                                                                                            cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGt                    <  1:2447602/148‑1 (MQ=255)
                                                                                                                              gCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTCTCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                  <  1:3089600/148‑1 (MQ=255)
                                                                                                                               ccAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAt                 <  2:3563925/148‑1 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATc                >  2:3214923/1‑148 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATc                <  2:3148337/148‑1 (MQ=255)
                                                                                                                                   tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg             <  2:3104027/148‑1 (MQ=255)
                                                                                                                                     tCGACTGAAGGCTTTCTGCCATGGTATGAAGTACTATCGGTAGGTGATAAACCAGCGTCAAGGTTATCGGTAGTTTGAATTCATCAGGTACCTGACAATGCGGAATAGTCCAGCCTCGCAGTAATCCATGACTGTCGCTGATCAGGtt           >  2:1889062/1‑148 (MQ=255)
                                                                                                                                       gCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtttt         <  1:1558571/148‑1 (MQ=255)
                                                                                                                                       gCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtttt         <  1:1000174/148‑1 (MQ=255)
                                                                                                                                           gTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTcc     <  1:2209413/148‑1 (MQ=255)
                                                                                                                                              ggCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAc  <  1:846266/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461354‑461643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑