Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 54,392 G→T 100% A104E (GCG→GAG)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927354,3920GT95.6% 547.2 / 15.3 182A104E (GCG→GAG) surApeptidyl‑prolyl cis‑trans isomerase (PPIase)
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (84/90);  total (88/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

GATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTG  >  CP009273/54247‑54536
                                                                                                                                                 |                                                                                                                                                
gATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTctcg                                                                                                                                                <  2:2257151/148‑1 (MQ=255)
gATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTctcg                                                                                                                                                >  2:1788458/1‑148 (MQ=255)
gATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTGGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCAGGTTGTTCTGTTTctcg                                                                                                                                                >  2:2876439/1‑148 (MQ=255)
  tGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGAt                                                                                                                                              >  2:1737024/1‑148 (MQ=255)
    gTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                            <  2:2077885/148‑1 (MQ=255)
     tGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGtt                                                                                                                                           <  2:3326562/148‑1 (MQ=255)
     tGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGtt                                                                                                                                           <  1:312632/148‑1 (MQ=255)
      gATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTa                                                                                                                                          >  1:1682598/1‑148 (MQ=255)
         gCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCa                                                                                                                                       >  1:2727020/1‑148 (MQ=255)
           gacgacGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAAt                                                                                                                                     <  1:2476698/148‑1 (MQ=255)
            acgacgCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATc                                                                                                                                    >  1:1673527/1‑148 (MQ=255)
               acgCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGcc                                                                                                                                 >  2:2558383/1‑148 (MQ=255)
                cgCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCt                                                                                                                                >  2:1727729/1‑148 (MQ=255)
                  cACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGa                                                                                                                              >  1:369950/1‑148 (MQ=255)
                    ccTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATc                                                                                                                            <  2:2302727/148‑1 (MQ=255)
                    ccTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATc                                                                                                                            <  1:2373115/148‑1 (MQ=255)
                      tCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCa                                                                                                                          >  2:1645009/1‑148 (MQ=255)
                          tgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctg                                                                                                                      >  1:445179/1‑148 (MQ=255)
                          tgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctg                                                                                                                      <  1:1110817/148‑1 (MQ=255)
                           gttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGGGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGAGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctgc                                                                                                                     >  2:2132488/1‑148 (MQ=255)
                            ttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctgct                                                                                                                    >  2:1031479/1‑148 (MQ=255)
                              aCGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCa                                                                                                                  <  1:1822583/148‑1 (MQ=255)
                               cGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCAt                                                                                                                 >  2:2109846/1‑148 (MQ=255)
                                   cTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGga                                                                                                             <  1:1213180/148‑1 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                            >  2:800103/1‑148 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                            >  1:1346682/1‑148 (MQ=255)
                                     tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                           <  1:2818006/148‑1 (MQ=255)
                                     tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                           >  1:3509043/1‑148 (MQ=255)
                                     tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                           <  1:653460/148‑1 (MQ=255)
                                     tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                           >  2:494342/1‑148 (MQ=255)
                                      cAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAt                                                                                                          <  1:1119465/148‑1 (MQ=255)
                                       agagATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAtt                                                                                                         <  1:315611/148‑1 (MQ=255)
                                         agaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAtttt                                                                                                       >  1:651705/1‑148 (MQ=255)
                                           aTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCa                                                                                                     >  2:1353644/1‑148 (MQ=255)
                                            tAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCAc                                                                                                    >  1:1686601/1‑148 (MQ=255)
                                             aaTCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACt                                                                                                   >  2:387725/1‑148 (MQ=255)
                                              atcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTc                                                                                                  >  2:3070090/1‑148 (MQ=255)
                                              atcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTc                                                                                                  >  2:3200631/1‑148 (MQ=255)
                                               tcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTcc                                                                                                 >  2:612134/1‑148 (MQ=255)
                                                catcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTccc                                                                                                <  2:2485263/148‑1 (MQ=255)
                                                catcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTccc                                                                                                >  2:2611637/1‑148 (MQ=255)
                                                catcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTccc                                                                                                <  1:1660078/148‑1 (MQ=255)
                                                  tctcTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAt                                                                                              >  2:1589045/1‑148 (MQ=255)
                                                    tctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAttt                                                                                            <  1:647527/148‑1 (MQ=255)
                                                    tctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAttt                                                                                            <  1:2347699/148‑1 (MQ=255)
                                                    tctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAttt                                                                                            <  1:2347346/148‑1 (MQ=255)
                                                       ttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCt                                                                                         >  2:1246914/1‑148 (MQ=255)
                                                       ttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCt                                                                                         >  1:2077959/1‑148 (MQ=255)
                                                        tGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTg                                                                                        <  1:161030/148‑1 (MQ=255)
                                                         gCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGc                                                                                       <  2:89287/148‑1 (MQ=255)
                                                           ggATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGccc                                                                                     <  1:1460975/148‑1 (MQ=255)
                                                              tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCAt                                                                                  >  1:1071062/1‑148 (MQ=255)
                                                              tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCAt                                                                                  <  1:3236822/148‑1 (MQ=255)
                                                              tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCAt                                                                                  >  1:3334039/1‑148 (MQ=255)
                                                               cTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATc                                                                                 >  1:510/1‑148 (MQ=255)
                                                               cTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCAAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATc                                                                                 >  1:2132051/1‑148 (MQ=255)
                                                                  gTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGc                                                                              >  2:3473229/1‑148 (MQ=255)
                                                                        aTAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatg                                                                        >  1:3562756/1‑148 (MQ=255)
                                                                        aTAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatg                                                                        >  1:3565701/1‑148 (MQ=255)
                                                                          aGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatgat                                                                      <  1:830894/148‑1 (MQ=255)
                                                                          aGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatgat                                                                      <  1:2406676/148‑1 (MQ=255)
                                                                          aGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatgat                                                                      <  1:2616143/148‑1 (MQ=255)
                                                                             tgttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTg                                                                   >  1:1936718/1‑148 (MQ=255)
                                                                               ttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGAt                                                                 >  1:724634/1‑148 (MQ=255)
                                                                               ttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTGTCTGCCCCATCTGCAGGATGATTTGAt                                                                 <  2:3254505/148‑1 (MQ=255)
                                                                                 gtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATcc                                                               <  1:2886957/148‑1 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                              >  2:3095241/1‑148 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                              <  2:73395/148‑1 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                              >  1:1073653/1‑148 (MQ=255)
                                                                                            cATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACg                                                    <  2:206562/148‑1 (MQ=255)
                                                                                             aTCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGt                                                   <  1:3230842/148‑1 (MQ=255)
                                                                                                  aaGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCAt                                              <  1:1900514/148‑1 (MQ=255)
                                                                                                  aaGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCAt                                              >  2:1262774/1‑148 (MQ=255)
                                                                                                   aGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATg                                             <  2:1071062/148‑1 (MQ=255)
                                                                                                     ccAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAt                                           <  1:1727800/148‑1 (MQ=255)
                                                                                                      cAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAtt                                          <  1:3200631/148‑1 (MQ=255)
                                                                                                      cAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAtt                                          <  1:2695873/148‑1 (MQ=255)
                                                                                                        gACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtg                                        <  2:946524/148‑1 (MQ=255)
                                                                                                        gACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtg                                        >  2:2521574/1‑148 (MQ=255)
                                                                                                         aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtgg                                       <  1:2280237/148‑1 (MQ=255)
                                                                                                           ggCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtggtg                                     >  1:162785/1‑148 (MQ=255)
                                                                                                           ggCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtggtg                                     <  1:1645009/148‑1 (MQ=255)
                                                                                                                   aTCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcg                             >  2:1141647/1‑148 (MQ=255)
                                                                                                                      tGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcg                          <  2:1683423/148‑1 (MQ=255)
                                                                                                                         tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                       >  1:542648/1‑148 (MQ=255)
                                                                                                                         tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                       >  1:151336/1‑148 (MQ=255)
                                                                                                                         tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTCTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                       >  1:2308480/1‑148 (MQ=255)
                                                                                                                            aGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtcat                    <  1:1685867/148‑1 (MQ=255)
                                                                                                                             gCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtcatc                   >  2:1375574/1‑148 (MQ=255)
                                                                                                                                tCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAgg                >  1:903705/1‑148 (MQ=255)
                                                                                                                                 cATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGa               <  1:2106648/148‑1 (MQ=255)
                                                                                                                                   tgttgtTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAg             <  2:1936718/148‑1 (MQ=255)
                                                                                                                                    gttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGt            <  1:2092478/148‑1 (MQ=255)
                                                                                                                                    gttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGt            <  1:2558383/148‑1 (MQ=255)
                                                                                                                                    gttgttCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGt            >  1:1067639/1‑148 (MQ=255)
                                                                                                                                     ttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGtt           >  2:2859008/1‑148 (MQ=255)
                                                                                                                                           tGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcc     <  2:2456684/148‑1 (MQ=255)
                                                                                                                                            gTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcct    >  2:1284544/1‑148 (MQ=255)
                                                                                                                                             tttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcctt   >  1:2864267/1‑148 (MQ=255)
                                                                                                                                              ttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgccttg  >  1:3365443/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTG  >  CP009273/54247‑54536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑