Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 479,244 C→A 100% A206S (GCC→TCC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273479,2440CA100.0% 630.8 / NA 206A206S (GCC→TCC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (116/90);  total (116/90)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCA  >  CP009273/479099‑479387
                                                                                                                                                 |                                                                                                                                               
ttCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                               >  2:3054053/1‑148 (MQ=255)
  cACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTa                                                                                                                                             <  1:2891617/148‑1 (MQ=255)
   aCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTaa                                                                                                                                            >  2:740375/1‑148 (MQ=255)
   aCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTaa                                                                                                                                            >  2:1437043/1‑148 (MQ=255)
      ttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGa                                                                                                                                         >  1:101330/1‑148 (MQ=255)
        cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACa                                                                                                                                       <  2:1595097/148‑1 (MQ=255)
        cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACa                                                                                                                                       <  1:1748330/148‑1 (MQ=255)
         agcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAt                                                                                                                                      <  1:1293825/148‑1 (MQ=255)
         agcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAt                                                                                                                                      >  2:1331303/1‑148 (MQ=255)
          gcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATc                                                                                                                                     >  1:115465/1‑148 (MQ=255)
           cagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCa                                                                                                                                    >  1:192031/1‑148 (MQ=255)
           cagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCa                                                                                                                                    >  2:3501456/1‑148 (MQ=255)
               aTTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAAATACATCAACCg                                                                                                                                >  1:2702641/1‑148 (MQ=255)
                 tttGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAAGAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGc                                                                                                                              <  2:1884586/148‑1 (MQ=255)
                  ttGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCg                                                                                                                             <  2:2732627/148‑1 (MQ=255)
                     ccGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTa                                                                                                                          <  1:1346673/148‑1 (MQ=255)
                      cGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAg                                                                                                                         <  2:3394403/148‑1 (MQ=255)
                       gAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGc                                                                                                                        >  1:1890305/1‑148 (MQ=255)
                       gAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTAACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTGTGATGGAGGTAATGACATCAACCCGCGTTAGc                                                                                                                        >  1:1890540/1‑148 (MQ=255)
                          ctctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTTATGGAGGTAATGACATAAACCCGCGGTAGCttg                                                                                                                     >  1:3002252/1‑146 (MQ=255)
                          ctctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCCGCGGTAGCTgg                                                                                                                     >  1:517879/1‑148 (MQ=255)
                           tctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGa                                                                                                                    <  2:2016113/148‑1 (MQ=255)
                           tctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGa                                                                                                                    >  2:1806049/1‑148 (MQ=255)
                           tctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGa                                                                                                                    <  1:1461607/148‑1 (MQ=255)
                            ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATTACATCAAACCGCGTTAGCTTGaa                                                                                                                   >  1:85200/1‑148 (MQ=255)
                             ttCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAt                                                                                                                  <  2:2630488/148‑1 (MQ=255)
                             ttCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAt                                                                                                                  <  2:1850068/148‑1 (MQ=255)
                                  tAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt                                                                                                             >  2:520871/1‑148 (MQ=255)
                                   agagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTc                                                                                                            <  2:816894/148‑1 (MQ=255)
                                    gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCa                                                                                                           >  2:2601857/1‑148 (MQ=255)
                                     agGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTTTTCAg                                                                                                          >  1:154372/1‑148 (MQ=255)
                                       gTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCt                                                                                                        >  2:2877628/1‑148 (MQ=255)
                                        tCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCCCCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTc                                                                                                       <  2:3093372/148‑1 (MQ=255)
                                        tCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTc                                                                                                       <  2:2537862/148‑1 (MQ=255)
                                           gACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt                                                                                                    >  2:63165/1‑148 (MQ=255)
                                            aCGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTAAGCTCATTc                                                                                                   >  1:2012248/1‑148 (MQ=255)
                                             cgcgTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCg                                                                                                  <  2:2404380/148‑1 (MQ=255)
                                                 tCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTAACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCTTTGTTGGAGGTAATGACATCAACCGGCGGTAGCTGGAAATTGTTAAGCTCATTAGGGtt                                                                                              >  1:1546102/1‑148 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                             >  2:1702429/1‑148 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                             <  2:31615/148‑1 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                             >  2:1584679/1‑148 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAACGGCGTTAGCTGGAATTTTTTCAGCTCAATCGGGTTc                                                                                             >  1:1708353/1‑148 (MQ=255)
                                                   tGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCa                                                                                            <  2:2977193/148‑1 (MQ=255)
                                                       cAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAAAAAAACCCGCGTTAGCTGGAATTTTTTAAGCTCATTAGGGTTCATACa                                                                                        >  1:333937/1‑148 (MQ=255)
                                                       cAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGAGGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATccc                                                                                        >  1:2676914/1‑147 (MQ=255)
                                                        aataatAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAg                                                                                       <  2:197241/148‑1 (MQ=255)
                                                          taataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAt                                                                                     >  2:1182902/1‑148 (MQ=255)
                                                              agagGCGTTAAGCTGTTGGCCATTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCTTTGATGGAGGTAATTAAAAAAACCGGCGTTAGCAGGAATTTGTTCAGCACATTCGGGTTCATACAGaaaagc                                                                                 >  1:1926737/1‑143 (MQ=255)
                                                               gagGCGTTAAGCTGTTTGCCTTTCACCGGCGGCGTACCACCCAGCTGACCCCCCGCAACCTGGGCGTTCTGCGCTTTTATGGATGTAATTAAATCAACCCGCGTTAGCTTGAATTTTTTAAGCTCATTAGGGTTCATACAGaaaacca                                                                                >  1:2211488/1‑142 (MQ=255)
                                                               gagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCa                                                                                >  2:3268890/1‑148 (MQ=255)
                                                                agGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCAt                                                                               >  2:3204767/1‑148 (MQ=255)
                                                                agGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCAt                                                                               >  2:2647786/1‑148 (MQ=255)
                                                                 ggCGTTAAGCTGTTGGCCTTTAACAGGCGGCGTACCACCGAGCTGACCCGCCGCAACATGGGCGTTCTGCGCTTTTATGGAGGTAATGACATAAAACGGAGTTAGCTGGAATTTGTTAAGCTAATTATGGTTCATCCAGATAatcatc                                                                              >  2:3038286/1‑148 (MQ=255)
                                                                  gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                             >  2:1285246/1‑148 (MQ=255)
                                                                     ttAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGt                                                                          >  2:470991/1‑148 (MQ=255)
                                                                      tAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTa                                                                         >  1:255511/1‑148 (MQ=255)
                                                                       aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTAc                                                                        >  2:2215409/1‑148 (MQ=255)
                                                                        aGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACt                                                                       >  2:2558426/1‑148 (MQ=255)
                                                                           tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgtg                                                                    <  2:192036/148‑1 (MQ=255)
                                                                           tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATACAGATACGCATCGCGTACtgtg                                                                    >  1:3324167/1‑148 (MQ=255)
                                                                             ttGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGaa                                                                  <  1:1850025/148‑1 (MQ=255)
                                                                              tGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAc                                                                 <  1:3351279/148‑1 (MQ=255)
                                                                                 ccTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGa                                                              >  2:3031762/1‑148 (MQ=255)
                                                                                 ccTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATAAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAccca                                                              >  1:230789/1‑146 (MQ=255)
                                                                                     tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaa                                                          <  1:951596/148‑1 (MQ=255)
                                                                                     tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaa                                                          <  2:356298/148‑1 (MQ=255)
                                                                                       aCCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACt                                                        >  2:68761/1‑148 (MQ=255)
                                                                                           gcggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAAc                                                    >  2:1227424/1‑148 (MQ=255)
                                                                                            cggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACa                                                   <  2:3566614/148‑1 (MQ=255)
                                                                                              gcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATc                                                 >  1:3140734/1‑148 (MQ=255)
                                                                                                 tACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCAcc                                              <  1:1437043/148‑1 (MQ=255)
                                                                                                       cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGcc                                        >  2:142176/1‑148 (MQ=255)
                                                                                                              aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTa                                 <  1:1776356/148‑1 (MQ=255)
                                                                                                              aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTa                                 >  2:715186/1‑148 (MQ=255)
                                                                                                              aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTa                                 >  2:3224734/1‑148 (MQ=255)
                                                                                                                 cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACgg                              <  1:285155/148‑1 (MQ=255)
                                                                                                                 cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACgg                              <  2:2937731/148‑1 (MQ=255)
                                                                                                                  gccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGc                             <  1:619767/148‑1 (MQ=255)
                                                                                                                   ccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                            >  1:816982/1‑148 (MQ=255)
                                                                                                                    cgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTg                           >  1:588820/1‑148 (MQ=255)
                                                                                                                       aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                        <  1:1702345/148‑1 (MQ=255)
                                                                                                                       aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                        <  1:1584679/148‑1 (MQ=255)
                                                                                                                        aCCTGGTCGTTCTGCTCTTTGATGGAGGTAATGAGATCAACCGGCTTTAGCTTGAATTTGTTCAGCTCATTCGGTTTCATCCAGATACGCATCGCGTACTGTGATCAGAACAACTGAACATCACCCCCGCCCGACGTACGGCTGATgg                       <  1:990658/148‑1 (MQ=255)
                                                                                                                        aCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATgg                       <  1:3543324/148‑1 (MQ=255)
                                                                                                                        aCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATgg                       >  1:525907/1‑148 (MQ=255)
                                                                                                                        aCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATgg                       >  1:1980802/1‑148 (MQ=255)
                                                                                                                         ccTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGc                      >  1:484087/1‑148 (MQ=255)
                                                                                                                          cTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCa                     <  1:2633369/148‑1 (MQ=255)
                                                                                                                          cTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCa                     <  2:2979654/148‑1 (MQ=255)
                                                                                                                            gggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATc                   <  1:1626272/148‑1 (MQ=255)
                                                                                                                              gCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCtt                 <  2:1646549/148‑1 (MQ=255)
                                                                                                                              gCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCtt                 <  1:1228577/148‑1 (MQ=255)
                                                                                                                                  tCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat             >  2:2780493/1‑148 (MQ=255)
                                                                                                                                     gcgcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAtt          >  2:3288703/1‑148 (MQ=255)
                                                                                                                                       gcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcg        <  1:470991/148‑1 (MQ=255)
                                                                                                                                       gcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcg        <  2:1623047/148‑1 (MQ=255)
                                                                                                                                         tttGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgcc      >  1:2108802/1‑148 (MQ=255)
                                                                                                                                         tttGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgcc      >  2:1812367/1‑148 (MQ=255)
                                                                                                                                             aTGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCa  >  2:2482512/1‑148 (MQ=255)
                                                                                                                                             aTGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCa  <  1:1182902/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCA  >  CP009273/479099‑479387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑