Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 55,590 G→T 100% T507K (ACG→AAG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927355,5900GT98.1% 633.1 / 3.2 213T507K (ACG→AAG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (90/119);  total (91/122)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

GCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCT  >  CP009273/55446‑55734
                                                                                                                                                |                                                                                                                                                
gCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCtttt                                                                                                                                               >  1:1229293/1‑148 (MQ=255)
     ggCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGta                                                                                                                                          <  2:2345252/148‑1 (MQ=255)
     ggCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGta                                                                                                                                          <  2:875019/148‑1 (MQ=255)
      gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtat                                                                                                                                         <  1:3206344/148‑1 (MQ=255)
       cAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtata                                                                                                                                        >  1:529931/1‑148 (MQ=255)
        aaTACGGTCAAGACCTCCGTAAGTCCGGTCCCGGAACAGGACAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATaa                                                                                                                                       <  2:16639/148‑1 (MQ=255)
         aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAc                                                                                                                                      >  1:798604/1‑148 (MQ=255)
         aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAc                                                                                                                                      >  1:798579/1‑148 (MQ=255)
          tACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAcc                                                                                                                                     <  1:966826/148‑1 (MQ=255)
           aCGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAccc                                                                                                                                    >  2:2878331/1‑148 (MQ=255)
           aCGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAccc                                                                                                                                    >  2:3459382/1‑148 (MQ=255)
            cGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCg                                                                                                                                   >  2:2936791/1‑148 (MQ=255)
            cGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCg                                                                                                                                   >  2:1561333/1‑148 (MQ=255)
            cGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCg                                                                                                                                   <  2:3314908/148‑1 (MQ=255)
                cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGc                                                                                                                               >  1:2536533/1‑148 (MQ=255)
                    aCCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGc                                                                                                                           >  1:2045040/1‑148 (MQ=255)
                       gccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAtt                                                                                                                        <  2:2160644/148‑1 (MQ=255)
                        ccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAttt                                                                                                                       <  2:3240530/148‑1 (MQ=255)
                         cgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTc                                                                                                                      >  1:1116538/1‑148 (MQ=255)
                         cgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTc                                                                                                                      >  1:1417046/1‑148 (MQ=255)
                         cgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTc                                                                                                                      >  1:1337354/1‑148 (MQ=255)
                          gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTcc                                                                                                                     <  2:2137028/148‑1 (MQ=255)
                            aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                   >  2:3427821/1‑148 (MQ=255)
                            aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                   <  1:637822/148‑1 (MQ=255)
                            aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                   >  2:27765/1‑148 (MQ=255)
                            aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                   >  1:2015182/1‑148 (MQ=255)
                            aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                   >  2:27047/1‑148 (MQ=255)
                              gTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                 <  1:2461031/148‑1 (MQ=255)
                                  ggTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcgcg                                                                                                             <  1:3382843/148‑1 (MQ=255)
                                    tCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTCGTTAAAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCACCTTTTGGGTATAACCCGGAGCCAGCATTTCCAAATCGCGtt                                                                                                           >  1:2983299/1‑148 (MQ=255)
                                          aaCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGa                                                                                                     >  1:1665735/1‑148 (MQ=255)
                                          aaCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGa                                                                                                     >  2:2563093/1‑148 (MQ=255)
                                               gCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtt                                                                                                >  2:2330455/1‑148 (MQ=255)
                                               gCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtt                                                                                                <  2:3401023/148‑1 (MQ=255)
                                               gCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtt                                                                                                >  1:2924552/1‑148 (MQ=255)
                                                ccAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAttt                                                                                               <  1:996775/148‑1 (MQ=255)
                                                ccAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAttt                                                                                               <  1:2777557/148‑1 (MQ=255)
                                                  agagTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTg                                                                                             <  1:3514948/148‑1 (MQ=255)
                                                      tagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                         >  2:2438743/1‑148 (MQ=255)
                                                      tagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                         >  1:1518174/1‑148 (MQ=255)
                                                      tagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                         >  1:871785/1‑148 (MQ=255)
                                                         tCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                                      <  1:3389287/148‑1 (MQ=255)
                                                          cAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                     >  1:1140910/1‑148 (MQ=255)
                                                           aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTGGCCGTCAACt                                                                                    <  2:387697/148‑1 (MQ=255)
                                                            gATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACtt                                                                                   >  1:2396812/1‑148 (MQ=255)
                                                               tGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGa                                                                                <  1:100398/148‑1 (MQ=255)
                                                                gcagcaGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTTCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGaa                                                                               <  2:1203188/148‑1 (MQ=255)
                                                                    cagAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGc                                                                           <  1:671989/148‑1 (MQ=255)
                                                                      gagaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                                         >  2:56804/1‑148 (MQ=255)
                                                                      gagaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                                         <  1:1976189/148‑1 (MQ=255)
                                                                       agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGc                                                                        <  2:1480629/148‑1 (MQ=255)
                                                                        gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCa                                                                       <  2:3566295/148‑1 (MQ=255)
                                                                         aCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                      <  2:525570/148‑1 (MQ=255)
                                                                               cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcgcg                                                                >  1:2114386/1‑148 (MQ=255)
                                                                                gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTTGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                                               <  2:3270006/148‑1 (MQ=255)
                                                                                 tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGt                                                              <  2:1438945/148‑1 (MQ=255)
                                                                                    ttATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                                           >  2:1343988/1‑148 (MQ=255)
                                                                                     tataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAAc                                                          >  2:562008/1‑148 (MQ=255)
                                                                                       taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACgg                                                        >  1:1178825/1‑148 (MQ=255)
                                                                                        aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                       >  2:3330896/1‑148 (MQ=255)
                                                                                        aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                       <  1:2066071/148‑1 (MQ=255)
                                                                                         gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAt                                                      <  2:3382282/148‑1 (MQ=255)
                                                                                            gTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                                   <  2:730145/148‑1 (MQ=255)
                                                                                             tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGt                                                  >  1:3450010/1‑148 (MQ=255)
                                                                                              cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTc                                                 <  1:1152942/148‑1 (MQ=255)
                                                                                                ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                               >  1:3155555/1‑148 (MQ=255)
                                                                                                 tctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAg                                              <  2:406975/148‑1 (MQ=255)
                                                                                                  ctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                             <  1:297421/148‑1 (MQ=255)
                                                                                                  ctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                             <  1:3041715/148‑1 (MQ=255)
                                                                                                    gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCGAGCTTTTGGGTATAACACGGAGCCAGCATTTCCATATCGCGTTGATAGACCTTTTTGCCGTCAACTTTTAATTTCGGCATTACGCGGTTAAAGGATTCGTCCAGCtt                                           <  1:3500754/148‑1 (MQ=255)
                                                                                                    gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCtt                                           >  2:2061522/1‑148 (MQ=255)
                                                                                                     aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTg                                          <  1:3162692/148‑1 (MQ=255)
                                                                                                      tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTgg                                         >  1:2671061/1‑148 (MQ=255)
                                                                                                      tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTgg                                         <  2:1427697/148‑1 (MQ=255)
                                                                                                       cgcgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                                        >  2:2444435/1‑148 (MQ=255)
                                                                                                            tACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                                   <  1:1888258/148‑1 (MQ=255)
                                                                                                               ggCACGTACCAAGACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                <  2:1869627/148‑1 (MQ=255)
                                                                                                               ggCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                <  2:2924552/148‑1 (MQ=255)
                                                                                                                 cACGTACAAATACTGCGCGCGCTGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATCTCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTTCGCGGTTAACGGATTCGTCCAGCTTGGTAGTGTTTCTg                              <  2:3055523/148‑1 (MQ=255)
                                                                                                                     tACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt                          <  2:2992880/148‑1 (MQ=255)
                                                                                                                      aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                         <  2:22081/148‑1 (MQ=255)
                                                                                                                      aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                         <  1:926382/148‑1 (MQ=255)
                                                                                                                      aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                         <  2:19122/148‑1 (MQ=255)
                                                                                                                      aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                         <  2:798579/148‑1 (MQ=255)
                                                                                                                      aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                         <  2:798604/148‑1 (MQ=255)
                                                                                                                       cAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta                        >  1:314738/1‑148 (MQ=255)
                                                                                                                        aaaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtat                       <  2:1140910/148‑1 (MQ=255)
                                                                                                                         aaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtata                      <  2:529932/148‑1 (MQ=255)
                                                                                                                         aaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtata                      >  1:436457/1‑148 (MQ=255)
                                                                                                                            aCTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCa                   <  2:951773/148‑1 (MQ=255)
                                                                                                                            aCTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCa                   >  2:2503087/1‑148 (MQ=255)
                                                                                                                              tGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGt                 <  1:1736978/148‑1 (MQ=255)
                                                                                                                                  cgcgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAg             <  1:2259567/148‑1 (MQ=255)
                                                                                                                                      cgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTNGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTg         <  1:1805661/148‑1 (MQ=255)
                                                                                                                                         ttCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGtt      <  2:1337354/148‑1 (MQ=255)
                                                                                                                                         ttCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGtt      >  2:738824/1‑148 (MQ=255)
                                                                                                                                         ttCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGtt      >  1:1769509/1‑148 (MQ=255)
                                                                                                                                          tCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGttt     >  2:2765179/1‑148 (MQ=255)
                                                                                                                                          tCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGttt     >  2:1557482/1‑148 (MQ=255)
                                                                                                                                           ccAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTg    <  2:2114386/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCT  >  CP009273/55446‑55734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑