Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 503,722 T→G 5.3% Y17D (TAT→GAT)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,7220TG 5.3% 656.8 / 27.0 225Y17D (TAT→GAT) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (117/96);  new base G (3/9);  total (120/105)
Fisher's exact test for biased strand distribution p-value = 7.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 230 total aligned reads displayed.

AGGGATGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACC  >  CP009273/503576‑503868
                                                                                                                                                  |                                                                                                                                                  
aGGGATGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTa                                                                                                                                                   >  1:1895323/1‑148 (MQ=255)
  ggATGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATg                                                                                                                                                 >  2:2170179/1‑148 (MQ=255)
   gtgggCGCAAATGGTGTTGGAGACTTGTTTAATGTGTTTGTATGATCCATTCTGTTCTTGCATCGCTATCCACACGCAACCAACTTTTAAAACCCATGAAACAGGCAACAACAAACCCGACGACACCCGGAGATATTCTTCTCTATGa                                                                                                                                                <  1:2764763/145‑1 (MQ=255)
   gATGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGa                                                                                                                                                >  1:3195247/1‑148 (MQ=255)
    aTGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGaa                                                                                                                                               >  1:54035/1‑148 (MQ=255)
        cgcAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATc                                                                                                                                           >  1:2325763/1‑148 (MQ=255)
           aaaTGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTgg                                                                                                                                        <  1:1159668/148‑1 (MQ=255)
            aaTGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGa                                                                                                                                       >  2:1528582/1‑148 (MQ=255)
            aaTGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGa                                                                                                                                       >  2:332903/1‑148 (MQ=255)
             aTGGAGTTGGAGACTTGTTTATTGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGaa                                                                                                                                      <  1:2659200/148‑1 (MQ=255)
             aTGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGaa                                                                                                                                      <  1:2658967/148‑1 (MQ=255)
                gAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCg                                                                                                                                   <  2:1895323/148‑1 (MQ=255)
                   ttGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTc                                                                                                                                >  1:646673/1‑148 (MQ=255)
                     ggAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGa                                                                                                                              >  1:2485623/1‑148 (MQ=255)
                       agaCTTGTTTAATTTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACAATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGAtt                                                                                                                            <  2:477270/148‑1 (MQ=255)
                       agaCTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGAtt                                                                                                                            <  2:281457/148‑1 (MQ=255)
                       agaCTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGAtt                                                                                                                            >  2:3389306/1‑148 (MQ=255)
                              tttAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAt                                                                                                                     >  1:1575511/1‑148 (MQ=255)
                                 attgtgTTTGTATTATTCAGTTTGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGAAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCaa                                                                                                                  <  2:3427922/146‑1 (MQ=255)
                                   tgtgTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATg                                                                                                                >  2:248455/1‑148 (MQ=255)
                                   tgtgTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGAACCGCTCGATTTGAAAATCAATg                                                                                                                >  1:1100407/1‑148 (MQ=255)
                                    gtgtTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGa                                                                                                               <  1:3491207/148‑1 (MQ=255)
                                     tgtTTTTATGATTCAGTATGTTATTGCAACGCTATTCACAAGCAAGCAACTTTTAAAAACAATGAAACAGGAAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAg                                                                                                              <  2:2103916/148‑1 (MQ=255)
                                     tgtTTGTATGATTCATTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATCAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAg                                                                                                              <  1:1718940/148‑1 (MQ=255)
                                     tgtTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAg                                                                                                              >  2:1234405/1‑148 (MQ=255)
                                      gtTTGTATGATTCTGTTTGTTCTTGCATCGCTATACACAAGGAAGCAACATTTAAAAACCATCAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGt                                                                                                             <  1:3048600/148‑1 (MQ=255)
                                           tATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCa                                                                                                        >  1:3566547/1‑148 (MQ=255)
                                           tATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCa                                                                                                        >  1:1804641/1‑148 (MQ=255)
                                            aTGATTCAGTATGTTCTTGCATTGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCag                                                                                                       >  2:1664681/1‑148 (MQ=255)
                                             tGATTCATTATTTTCTTGCATCGCTATTCACAAGGAAGCAACAGTAAAAAACCATGAAACAGGCACCAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCaga                                                                                                      <  1:850459/148‑1 (MQ=255)
                                              gATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCagag                                                                                                     <  2:2231948/148‑1 (MQ=255)
                                               aTTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGt                                                                                                    >  2:437307/1‑148 (MQ=255)
                                               aTTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGt                                                                                                    >  2:550461/1‑148 (MQ=255)
                                                   ttttttttCTTGCATCTATATTCAAAAGTAATCAACATTTAAAAACCATTAAACAGCAAAAAATAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTtgct                                                                                                <  1:2183429/142‑1 (MQ=255)
                                                      aTGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCa                                                                                             <  2:2571773/148‑1 (MQ=255)
                                                       tGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCAt                                                                                            >  2:2867531/1‑148 (MQ=255)
                                                        gTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGTAACCGCTCGATTTGAAAATCAATGAGTTATCAGAGTTGCTGCATg                                                                                           >  1:375594/1‑148 (MQ=255)
                                                         ttCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGt                                                                                          >  1:947507/1‑148 (MQ=255)
                                                           cTTGCATCGCTATACACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTc                                                                                        <  1:469643/148‑1 (MQ=255)
                                                            ttGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                                                                       >  1:512354/1‑148 (MQ=255)
                                                             tGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGCACCGCGCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCAt                                                                                      >  1:3329894/1‑148 (MQ=255)
                                                               cATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCg                                                                                    >  2:562407/1‑148 (MQ=255)
                                                               cATCGCTATTAACAAGGAACAAAAAGTAAAAAACCATGAAACAGGCAACAATAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCg                                                                                    <  2:3024893/148‑1 (MQ=255)
                                                                 tCGTTATTCACAAGGAAGCAACATTTAAATACAATTAAACTGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTa                                                                                  <  2:1275053/148‑1 (MQ=255)
                                                                 tCGCTATTCACTAGGATGCAAGAGTTAAAAACCATGAAACTGTCAACAAGAAAACCGTCGACACGCGGAGATATTCTTCTCTATGAATATCTGGAAGCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTa                                                                                  <  2:292572/148‑1 (MQ=255)
                                                                 tCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTa                                                                                  <  2:525293/148‑1 (MQ=255)
                                                                 tCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTa                                                                                  <  2:1100407/148‑1 (MQ=255)
                                                                  cGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTaa                                                                                 >  2:1584482/1‑148 (MQ=255)
                                                                  cGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTaa                                                                                 >  2:2028187/1‑148 (MQ=255)
                                                                   gCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAAt                                                                                >  2:3078559/1‑148 (MQ=255)
                                                                      aTTCACAAGTAAGCAACATTAAAAAACCATGAAACAGGCAACAATAAACCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGc                                                                             <  1:1024248/148‑1 (MQ=255)
                                                                          acaAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCa                                                                         >  2:1424661/1‑148 (MQ=255)
                                                                          acaAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCa                                                                         <  2:2847126/148‑1 (MQ=255)
                                                                              ggAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGc                                                                     >  2:1972148/1‑148 (MQ=255)
                                                                               gAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                    >  2:2359785/1‑148 (MQ=255)
                                                                               gAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                    >  2:2590346/1‑148 (MQ=255)
                                                                                aaGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                                                                   >  2:3177879/1‑148 (MQ=255)
                                                                                     aCAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATc                                                              >  1:1168619/1‑148 (MQ=255)
                                                                                       attaaaaaaCCATGAAACTGGCAACAAGAAACCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCaa                                                            <  1:2570691/144‑1 (MQ=255)
                                                                                       aGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCaa                                                            >  2:3477676/1‑148 (MQ=255)
                                                                                         ttAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATa                                                          >  1:2099739/1‑148 (MQ=255)
                                                                                         ttAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATa                                                          <  2:277466/148‑1 (MQ=255)
                                                                                          tAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGAGTCAGTGCACTGATCAATaa                                                         >  2:1248856/1‑148 (MQ=255)
                                                                                            aaaaCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACa                                                       <  2:1141105/148‑1 (MQ=255)
                                                                                             aaaCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACaa                                                      >  1:1400313/1‑148 (MQ=255)
                                                                                             aaaCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACaa                                                      >  2:3065168/1‑148 (MQ=255)
                                                                                               aCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATc                                                    >  1:2381387/1‑148 (MQ=255)
                                                                                                  aTGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTa                                                 >  1:1532327/1‑148 (MQ=255)
                                                                                                      aaCAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACt                                             >  2:547918/1‑148 (MQ=255)
                                                                                                       aCAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTc                                            >  2:1639377/1‑148 (MQ=255)
                                                                                                       aCAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTc                                            <  2:376276/148‑1 (MQ=255)
                                                                                                        cAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCa                                           >  1:3404950/1‑148 (MQ=255)
                                                                                                           gaaacaaGAAACCCGACGACACCCGGAGATATTCTTCTCGATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCacta                                        <  1:30097/146‑1 (MQ=255)
                                                                                                           gCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCacta                                        <  2:1572493/148‑1 (MQ=255)
                                                                                                            aaacaaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCactac                                       <  1:2568302/147‑1 (MQ=255)
                                                                                                               caaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTga                                    <  1:2863078/148‑1 (MQ=255)
                                                                                                               caaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATAGTAAAAGCTGCAGTGCACTTATCAAATACAATCGTAAACTAACTACTga                                    >  1:659717/1‑148 (MQ=255)
                                                                                                                 aGAAAACCGACGACACCCGGAGATTTTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTgaga                                  <  2:2269310/148‑1 (MQ=255)
                                                                                                                 aGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTgaga                                  <  1:2450699/148‑1 (MQ=255)
                                                                                                                 aGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTgaga                                  >  1:1650012/1‑148 (MQ=255)
                                                                                                                  gAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGAt                                 <  2:8895/148‑1 (MQ=255)
                                                                                                                  gAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGAt                                 >  2:1625319/1‑148 (MQ=255)
                                                                                                                   aaacCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATg                                <  1:2791860/148‑1 (MQ=255)
                                                                                                                   aaaaCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATg                                <  1:1292875/148‑1 (MQ=255)
                                                                                                                    aaaCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATgg                               <  1:3143334/148‑1 (MQ=255)
                                                                                                                      aCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCa                             <  1:159532/148‑1 (MQ=255)
                                                                                                                      aCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCa                             <  1:2479734/148‑1 (MQ=255)
                                                                                                                       ccGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCAt                            >  1:1079944/1‑148 (MQ=255)
                                                                                                                        cgacgaCACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCAtt                           <  1:1972148/148‑1 (MQ=255)
                                                                                                                          acgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTc                         <  2:239151/148‑1 (MQ=255)
                                                                                                                            gacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGt                       <  2:3371478/148‑1 (MQ=255)
                                                                                                                             acacCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTc                      <  2:646673/148‑1 (MQ=255)
                                                                                                                                    gagaTATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGaa               >  2:3286435/1‑148 (MQ=255)
                                                                                                                                      gaTATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAg             >  2:3232604/1‑148 (MQ=255)
                                                                                                                                        tatTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTAATCGTAATACCGTCAGTGCACAGATCAATAACAATCGTAAACACACTACAGAGATGGCATTTCGTCTGGCGAAAGtt           >  1:908715/1‑148 (MQ=255)
                                                                                                                                         atTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGttt          <  1:1526851/148‑1 (MQ=255)
                                                                                                                                          ttcttcTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGtttt         >  2:2983669/1‑148 (MQ=255)
                                                                                                                                             ttctCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGa      >  2:118652/1‑148 (MQ=255)
                                                                                                                                                 ctATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATAcc  >  2:3350848/1‑148 (MQ=255)
                                                                                                                                                 ctATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATAcc  >  2:437602/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGGGATGGCGCAAATGGAGTTGGAGACTTGTTTAATGTGTTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACC  >  CP009273/503576‑503868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑