Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 518,820 C→A 100% A35E (GCG→GAG)  rhsD → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273518,8200CA95.7% 560.9 / 14.9 186A35E (GCG→GAG) rhsDRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (1/7);  new base A (83/95);  total (84/102)
Fisher's exact test for biased strand distribution p-value = 7.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAC  >  CP009273/518675‑518964
                                                                                                                                                 |                                                                                                                                                
gCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                >  1:2418558/1‑148 (MQ=255)
 cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCACATTGTCAAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGgcgtggagtg                                                                                                                                               >  1:510743/1‑148 (MQ=255)
  tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGc                                                                                                                                              <  2:1198376/148‑1 (MQ=255)
  tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGc                                                                                                                                              <  2:2976431/148‑1 (MQ=255)
  tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGc                                                                                                                                              <  2:745885/148‑1 (MQ=255)
   aaCACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                             >  1:2877313/1‑148 (MQ=255)
   aaCACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                             <  2:857597/148‑1 (MQ=255)
    acacAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTc                                                                                                                                            <  1:554438/148‑1 (MQ=255)
        aGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtg                                                                                                                                        <  2:1800410/148‑1 (MQ=255)
                gAACAATAACTAAAAGGGCACTGTATATGAGCGGAAAACCAGAGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcggg                                                                                                                                >  2:2214858/1‑148 (MQ=255)
                 aaCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggc                                                                                                                               >  2:1436510/1‑148 (MQ=255)
                 aaCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggc                                                                                                                               <  1:338342/148‑1 (MQ=255)
                  aCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtg                                                                                                                                        <  2:339031/138‑1 (MQ=255)
                  aCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtg                                                                                                                                        >  1:339171/1‑138 (MQ=255)
                  aCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtg                                                                                                                                        >  1:339031/1‑138 (MQ=255)
                  aCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggcg                                                                                                                              >  2:391686/1‑148 (MQ=255)
                     aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGa                                                                                                                           >  1:887630/1‑148 (MQ=255)
                      tAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGAt                                                                                                                          >  1:1702157/1‑148 (MQ=255)
                       aaCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATg                                                                                                                         <  2:9598/148‑1 (MQ=255)
                         cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGAc                                                                                                                       <  2:2725453/148‑1 (MQ=255)
                         cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGAc                                                                                                                       >  1:1387503/1‑148 (MQ=255)
                         cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGAc                                                                                                                       <  2:1733591/148‑1 (MQ=255)
                          tAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACt                                                                                                                      <  2:2503870/148‑1 (MQ=255)
                          tAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACt                                                                                                                      <  1:3248627/148‑1 (MQ=255)
                              aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                  >  1:3511612/1‑148 (MQ=255)
                              aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                  >  1:1941606/1‑148 (MQ=255)
                                    cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAcc                                                                                                            <  1:1696673/148‑1 (MQ=255)
                                       tataTGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCgg                                                                                                         <  1:1412622/148‑1 (MQ=255)
                                        atatGAGCGGAAAACCAGCGGCGCGTCAGGGATATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGt                                                                                                        <  2:2352735/148‑1 (MQ=255)
                                        atatGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGt                                                                                                        >  1:783743/1‑148 (MQ=255)
                                          atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      >  2:2257684/1‑148 (MQ=255)
                                          atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      >  2:1717637/1‑148 (MQ=255)
                                           tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  1:225378/148‑1 (MQ=255)
                                           tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  1:1552668/148‑1 (MQ=255)
                                           tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  1:186764/148‑1 (MQ=255)
                                           tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  1:2026945/148‑1 (MQ=255)
                                           tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  1:1992025/148‑1 (MQ=255)
                                            gAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAAt                                                                                                    <  2:517116/148‑1 (MQ=255)
                                             aGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATc                                                                                                   <  1:1279356/148‑1 (MQ=255)
                                               cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCg                                                                                                 >  1:3393960/1‑148 (MQ=255)
                                                ggAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgc                                                                                                <  2:2697750/148‑1 (MQ=255)
                                                ggAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgc                                                                                                <  2:2696551/148‑1 (MQ=255)
                                                     aCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                           >  1:384851/1‑148 (MQ=255)
                                                     aCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                           >  1:2564310/1‑148 (MQ=255)
                                                            gcgcGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGGCTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGa                                                                                    >  1:857546/1‑148 (MQ=255)
                                                            gcgcGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGa                                                                                    >  1:2922748/1‑148 (MQ=255)
                                                               cgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAgg                                                                                 <  1:2666798/148‑1 (MQ=255)
                                                                gTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGt                                                                                <  1:2845944/148‑1 (MQ=255)
                                                                gTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGt                                                                                <  2:1562499/148‑1 (MQ=255)
                                                                 tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                               >  1:3179214/1‑148 (MQ=255)
                                                                 tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                               <  1:2710909/148‑1 (MQ=255)
                                                                  cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgc                                                                              >  2:30297/1‑148 (MQ=255)
                                                                  cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgc                                                                              >  1:3065667/1‑148 (MQ=255)
                                                                    gggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctg                                                                            <  2:755211/148‑1 (MQ=255)
                                                                    gggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                        >  1:2267877/1‑136 (MQ=255)
                                                                    gggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                        <  2:2267877/136‑1 (MQ=255)
                                                                     ggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                           <  1:2780035/148‑1 (MQ=255)
                                                                     ggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                           >  2:1511876/1‑148 (MQ=255)
                                                                      gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGcc                                                                          <  2:1001112/148‑1 (MQ=255)
                                                                      gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGcc                                                                          >  2:2244346/1‑148 (MQ=255)
                                                                      gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGcc                                                                          <  2:1937898/148‑1 (MQ=255)
                                                                           atGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCg                                                                     >  1:2992121/1‑148 (MQ=255)
                                                                           atGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCg                                                                     <  1:361474/148‑1 (MQ=255)
                                                                              aCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCgaga                                                                  <  1:444064/148‑1 (MQ=255)
                                                                               cTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGAc                                                                 >  1:578118/1‑148 (MQ=255)
                                                                                 cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACgg                                                               <  2:377507/148‑1 (MQ=255)
                                                                                 cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACgg                                                               <  2:374164/148‑1 (MQ=255)
                                                                                    tATTGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAcc                                                            >  1:12439/1‑148 (MQ=255)
                                                                                    tATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAcc                                                            <  2:3481483/148‑1 (MQ=255)
                                                                                    tATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAcc                                                            <  2:1707195/148‑1 (MQ=255)
                                                                                     aTGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCt                                                           <  1:1922348/148‑1 (MQ=255)
                                                                                         cGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgcg                                                       <  2:3351810/148‑1 (MQ=255)
                                                                                            tCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTg                                                    <  1:1025675/148‑1 (MQ=255)
                                                                                             cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgc                                                   >  2:2587255/1‑148 (MQ=255)
                                                                                             cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgc                                                   <  2:2533513/148‑1 (MQ=255)
                                                                                              ccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcc                                                  <  2:1031538/148‑1 (MQ=255)
                                                                                               cATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                 >  2:2098590/1‑148 (MQ=255)
                                                                                                  tGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggc                                              >  1:1963879/1‑148 (MQ=255)
                                                                                                   gTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcc                                             <  2:312802/148‑1 (MQ=255)
                                                                                                     ccAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                           <  1:2095154/148‑1 (MQ=255)
                                                                                                     ccAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                           <  1:295349/148‑1 (MQ=255)
                                                                                                          gTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGcc                                      >  1:1543063/1‑148 (MQ=255)
                                                                                                               gCAGGTGTAAGAAGTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCa                                 <  2:451813/148‑1 (MQ=255)
                                                                                                                cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCAt                                <  1:178056/148‑1 (MQ=255)
                                                                                                                cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCAt                                <  1:3314299/148‑1 (MQ=255)
                                                                                                                     gtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct                           >  1:1016984/1‑148 (MQ=255)
                                                                                                                     gtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct                           <  1:1640081/148‑1 (MQ=255)
                                                                                                                     gtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct                           <  1:35385/148‑1 (MQ=255)
                                                                                                                      tAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCGGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc                          <  2:1732998/148‑1 (MQ=255)
                                                                                                                      tAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc                          <  1:1593020/148‑1 (MQ=255)
                                                                                                                       aaGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTcc                         <  2:2418558/148‑1 (MQ=255)
                                                                                                                         gAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCg                       >  2:612283/1‑148 (MQ=255)
                                                                                                                         gAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCg                       >  1:2345031/1‑148 (MQ=255)
                                                                                                                          aaTTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc                      >  2:114948/1‑148 (MQ=255)
                                                                                                                          aaTTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc                      <  1:2489643/148‑1 (MQ=255)
                                                                                                                            ttGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCAc                    <  2:2110817/148‑1 (MQ=255)
                                                                                                                             tGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCAcc                   <  2:1878733/148‑1 (MQ=255)
                                                                                                                               gcgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTa                 >  1:2618902/1‑148 (MQ=255)
                                                                                                                                     ccACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagcag           <  1:2770624/148‑1 (MQ=255)
                                                                                                                                      cACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc          <  2:2779478/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAC  >  CP009273/518675‑518964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑