Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 551,602 A→T 61.0% F136L (TTT→TTA)  ybcI ← DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273551,6020AT61.0% 169.6 / 240.0 197F136L (TTT→TTA) ybcIDUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (45/32);  new base T (73/47);  total (118/79)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

GCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGA  >  CP009273/551457‑551749
                                                                                                                                                 |                                                                                                                                                   
gcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCaaa                                                                                                                                                   <  1:115037/148‑1 (MQ=255)
  tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACg                                                                                                                                                 >  1:939073/1‑148 (MQ=255)
  tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACg                                                                                                                                                 >  1:2460834/1‑148 (MQ=255)
  tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACg                                                                                                                                                 >  2:2028091/1‑148 (MQ=255)
  tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACg                                                                                                                                                 >  2:648940/1‑148 (MQ=255)
       ccGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGcgcg                                                                                                                                            >  2:3223845/1‑148 (MQ=255)
          gATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACt                                                                                                                                         <  1:2087177/148‑1 (MQ=255)
          gATGCGAGTGTTCGCATCCGGTTATCGTCTGCTCCACCACAACATGCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACt                                                                                                                                         <  2:1886350/148‑1 (MQ=255)
           aTGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtt                                                                                                                                        >  2:1612648/1‑148 (MQ=255)
           aTGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtt                                                                                                                                        <  2:523950/148‑1 (MQ=255)
             gCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACtttt                                                                                                                                      >  2:240741/1‑148 (MQ=255)
              cGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa                                                                                                                                     <  1:1930452/148‑1 (MQ=255)
                aGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATc                                                                                                                                   >  1:3172046/1‑148 (MQ=255)
                  ttttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCAc                                                                                                                                 <  1:709993/148‑1 (MQ=255)
                  ttttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc                                                                                                                                 >  2:2656072/1‑148 (MQ=255)
                   tttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc                                                                                                                                <  1:2577918/148‑1 (MQ=255)
                      cGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGc                                                                                                                             <  2:2390990/148‑1 (MQ=255)
                        cATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCa                                                                                                                           <  1:1673742/148‑1 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         <  2:1134352/148‑1 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  2:1180525/1‑148 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  2:2847298/1‑148 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  1:3404080/1‑148 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         <  1:1432299/148‑1 (MQ=255)
                          tCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  1:1967963/1‑148 (MQ=255)
                           ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg                                                                                                                        >  2:878290/1‑148 (MQ=255)
                           ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAggg                                                                                                                        <  1:3066853/148‑1 (MQ=255)
                            cGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGTTGTAACGAGACAATGATAACGGCGCGACTTTTATCACATGCCAGGGa                                                                                                                       >  2:2816677/1‑148 (MQ=255)
                                tATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGa                                                                                                                   >  1:3546941/1‑148 (MQ=255)
                                 aTCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaa                                                                                                                  >  1:1749038/1‑148 (MQ=255)
                                   cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa                                                                                                                >  1:2489074/1‑148 (MQ=255)
                                    gTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaaa                                                                                                               <  2:1158076/148‑1 (MQ=255)
                                    gTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaaa                                                                                                               >  2:1330445/1‑148 (MQ=255)
                                         ctccaccaCACCCTTCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGtt                                                                                                          <  2:1303820/146‑1 (MQ=255)
                                         ctccaccaCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCACAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGtt                                                                                                          <  1:262621/146‑1 (MQ=255)
                                           ccaccaCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCa                                                                                                        >  1:1999789/1‑148 (MQ=255)
                                           ccaccaCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATACACAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCa                                                                                                        <  1:3187022/148‑1 (MQ=255)
                                           ccaccaCAAAATTCCCATCATTAGAATCCCCGGCAACAACACCCAAATCAATTCACAAATAATCCCCTGATGCCCGTACGGGGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCa                                                                                                        <  1:2099674/148‑1 (MQ=255)
                                            caccacAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACAACAATTCAGAAATAATCACCTGATGCCCGTCCGGCGTGGTGTACCGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAt                                                                                                       <  1:387963/148‑1 (MQ=255)
                                            caccacAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAt                                                                                                       <  2:473436/148‑1 (MQ=255)
                                               cacaACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTg                                                                                                    >  2:1301879/1‑148 (MQ=255)
                                               cacaACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTg                                                                                                    >  2:1568660/1‑148 (MQ=255)
                                               cacaACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTg                                                                                                    <  1:1012643/148‑1 (MQ=255)
                                                acaacaTTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGa                                                                                                   <  2:280457/148‑1 (MQ=255)
                                                      ttCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTCTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACgg                                                                                             >  1:1273585/1‑148 (MQ=255)
                                                                tAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagccag                                                                                   <  2:17496/148‑1 (MQ=255)
                                                                 aGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagccagc                                                                                  >  2:587277/1‑148 (MQ=255)
                                                                   cATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                                <  1:2678521/148‑1 (MQ=255)
                                                                     tCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAAc                                                                              >  1:227942/1‑148 (MQ=255)
                                                                      ccccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACg                                                                             >  2:2781781/1‑148 (MQ=255)
                                                                        ccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                           >  1:1462989/1‑148 (MQ=255)
                                                                        ccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                           <  2:1857895/148‑1 (MQ=255)
                                                                         cGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCACCTGACCACGGCCACAGCCAGCCAACGCCt                                                                          >  1:1769034/1‑148 (MQ=255)
                                                                          ggCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCtt                                                                         >  1:2660276/1‑148 (MQ=255)
                                                                             aaCCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTAc                                                                      <  2:1793551/148‑1 (MQ=255)
                                                                               ccACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCg                                                                    >  2:1942453/1‑148 (MQ=255)
                                                                               ccACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCg                                                                    >  2:2348756/1‑148 (MQ=255)
                                                                                 acacCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGcc                                                                  <  1:1302181/148‑1 (MQ=255)
                                                                                    cccACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGt                                                               >  1:2036157/1‑148 (MQ=255)
                                                                                     ccACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTg                                                              >  1:2923077/1‑148 (MQ=255)
                                                                                      cacaTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTgg                                                             <  1:1477582/148‑1 (MQ=255)
                                                                                       acaTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGt                                                            <  2:942308/148‑1 (MQ=255)
                                                                                        caTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGtt                                                           >  1:2185022/1‑148 (MQ=255)
                                                                                          tCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACTGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGt                                                            <  1:2047122/145‑1 (MQ=255)
                                                                                          tCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTAc                                                         >  1:1240105/1‑148 (MQ=255)
                                                                                          tCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTAACACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGt                                                            >  2:2047122/1‑145 (MQ=255)
                                                                                           cAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACAGCCAGTGGTTAcc                                                        >  2:3366752/1‑148 (MQ=255)
                                                                                             aTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGa                                                      >  1:1424989/1‑148 (MQ=255)
                                                                                              ttCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTCCCGaa                                                     >  2:2786786/1‑148 (MQ=255)
                                                                                                cAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATc                                                   <  2:2909268/148‑1 (MQ=255)
                                                                                                 aGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATcc                                                  >  1:3434611/1‑148 (MQ=255)
                                                                                                 aGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATcc                                                  >  1:3434629/1‑148 (MQ=255)
                                                                                                    aataatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGc                                               >  1:1729417/1‑148 (MQ=255)
                                                                                                     ataatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGCGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCa                                              >  1:687092/1‑148 (MQ=255)
                                                                                                       aatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAg                                            >  1:1464040/1‑148 (MQ=255)
                                                                                                       aatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAg                                            >  1:2276430/1‑148 (MQ=255)
                                                                                                         tCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCt                                          >  1:555449/1‑148 (MQ=255)
                                                                                                          cACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCtg                                         >  1:3511478/1‑148 (MQ=255)
                                                                                                            ccTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCtgtg                                       <  1:2857553/148‑1 (MQ=255)
                                                                                                              tGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCg                                     <  1:2847298/148‑1 (MQ=255)
                                                                                                                aTGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGAt                                   >  2:695658/1‑148 (MQ=255)
                                                                                                                aTGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGAt                                   >  1:2874748/1‑148 (MQ=255)
                                                                                                                aTGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGAt                                   >  2:286103/1‑148 (MQ=255)
                                                                                                                aTGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGAt                                   <  1:1044897/148‑1 (MQ=255)
                                                                                                                     cGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCaa                              <  1:2355323/148‑1 (MQ=255)
                                                                                                                      gTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAAc                             >  2:3128512/1‑148 (MQ=255)
                                                                                                                       tACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACg                            <  1:648940/148‑1 (MQ=255)
                                                                                                                        aCGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACga                           >  1:638212/1‑148 (MQ=255)
                                                                                                                            cGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACg                       >  2:831792/1‑148 (MQ=255)
                                                                                                                              tggtgTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGt                     >  1:1247776/1‑148 (MQ=255)
                                                                                                                                   tAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaa                >  1:310914/1‑148 (MQ=255)
                                                                                                                                   tAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaa                >  2:2157931/1‑148 (MQ=255)
                                                                                                                                   tAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaa                >  1:3542946/1‑148 (MQ=255)
                                                                                                                                    aaCGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaaa               >  2:255604/1‑148 (MQ=255)
                                                                                                                                     aCGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc              <  1:3272711/148‑1 (MQ=255)
                                                                                                                                     aCGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc              <  1:454587/148‑1 (MQ=255)
                                                                                                                                     aCGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc              <  1:455003/148‑1 (MQ=255)
                                                                                                                                      cGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAca             >  1:1411384/1‑148 (MQ=255)
                                                                                                                                         gaCAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcagcc          <  1:595149/148‑1 (MQ=255)
                                                                                                                                            aaTGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcagccagc       >  1:2508751/1‑148 (MQ=255)
                                                                                                                                                 aaaCGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGa  <  2:1468139/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGA  >  CP009273/551457‑551749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑