Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 601,847 A→T 100% I331I (ATT→ATA)  ybdK ← weak gamma‑glutamyl:cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273601,8470AT98.2% 715.7 / 2.0 220I331I (ATT→ATA) ybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base T (110/106);  total (110/110)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

GTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGG  >  CP009273/601704‑601991
                                                                                                                                               |                                                                                                                                                
gTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcg                                                                                                                                              >  2:2453772/1‑148 (MQ=255)
  aaGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcgcg                                                                                                                                            <  2:878344/148‑1 (MQ=255)
      cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCg                                                                                                                                        >  2:1411805/1‑148 (MQ=255)
      cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCg                                                                                                                                        >  1:2190476/1‑148 (MQ=255)
        aTTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGAt                                                                                                                                      >  1:3093470/1‑148 (MQ=255)
         ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATg                                                                                                                                     <  2:523826/148‑1 (MQ=255)
         ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATg                                                                                                                                     >  2:3102113/1‑148 (MQ=255)
         ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATAGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATg                                                                                                                                     >  1:3299206/1‑148 (MQ=255)
          tCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGc                                                                                                                                    >  1:244502/1‑148 (MQ=255)
            ccGGCTTAGTCCCCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCAc                                                                                                                                  <  2:2577120/148‑1 (MQ=255)
                  tAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAttt                                                                                                                            <  1:1011381/148‑1 (MQ=255)
                   aGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAtttt                                                                                                                           <  2:3278526/148‑1 (MQ=255)
                   aGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAtttt                                                                                                                           >  2:1742138/1‑148 (MQ=255)
                    gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTa                                                                                                                          >  1:472880/1‑148 (MQ=255)
                    gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTa                                                                                                                          >  1:3468095/1‑148 (MQ=255)
                     tCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAt                                                                                                                         <  1:1375822/148‑1 (MQ=255)
                      cACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                        <  2:1695142/148‑1 (MQ=255)
                      cACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                        >  1:2898264/1‑148 (MQ=255)
                            cccAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGa                                                                                                                  <  1:768634/148‑1 (MQ=255)
                             ccAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGaa                                                                                                                 <  1:2771400/148‑1 (MQ=255)
                              cAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAg                                                                                                                >  2:3207829/1‑148 (MQ=255)
                               aGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAgg                                                                                                               <  1:139190/148‑1 (MQ=255)
                               aGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAgg                                                                                                               <  2:2512159/148‑1 (MQ=255)
                                gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                              >  1:593668/1‑148 (MQ=255)
                                 aTCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAgggg                                                                                                             >  2:2061514/1‑148 (MQ=255)
                                 aTCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAgggg                                                                                                             >  1:226758/1‑148 (MQ=255)
                                  tctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGc                                                                                                            <  1:144836/148‑1 (MQ=255)
                                   ctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCg                                                                                                           >  1:1659121/1‑148 (MQ=255)
                                   ctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCg                                                                                                           >  1:3427272/1‑148 (MQ=255)
                                   ctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCg                                                                                                           <  2:3399050/148‑1 (MQ=255)
                                   ctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCg                                                                                                           <  1:3307116/148‑1 (MQ=255)
                                    tcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGa                                                                                                          <  2:2884681/148‑1 (MQ=255)
                                    tcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGa                                                                                                          <  2:1105282/148‑1 (MQ=255)
                                     cacaATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAt                                                                                                         >  2:2717594/1‑148 (MQ=255)
                                      acaATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtt                                                                                                        <  2:1144639/148‑1 (MQ=255)
                                       caATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttt                                                                                                       <  2:258281/148‑1 (MQ=255)
                                        aaggCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtttt                                                                                                      <  2:1072839/145‑1 (MQ=255)
                                        aaTGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtttt                                                                                                      <  2:1750706/148‑1 (MQ=255)
                                         aTGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttttt                                                                                                     <  2:274920/148‑1 (MQ=255)
                                                   aCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATc                                                                                           >  2:1158031/1‑148 (MQ=255)
                                                      aGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAAtagaa                                                                                        >  1:314099/1‑144 (MQ=255)
                                                        cccAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAg                                                                                      <  2:2920415/148‑1 (MQ=255)
                                                         ccAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAgg                                                                                     <  2:2183264/148‑1 (MQ=255)
                                                          cAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGt                                                                                    >  1:278747/1‑148 (MQ=255)
                                                          cAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGt                                                                                    >  1:1572104/1‑148 (MQ=255)
                                                            aTCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTAt                                                                                  >  1:1178886/1‑148 (MQ=255)
                                                            aTCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTAt                                                                                  >  2:33079/1‑148 (MQ=255)
                                                             tCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATc                                                                                 >  1:2865111/1‑148 (MQ=255)
                                                             tCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATc                                                                                 <  1:818466/148‑1 (MQ=255)
                                                              cAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCt                                                                                <  1:15052/148‑1 (MQ=255)
                                                                 cGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTcc                                                                             <  1:1068733/148‑1 (MQ=255)
                                                                 cGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTcc                                                                             >  1:2066830/1‑148 (MQ=255)
                                                                  gAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCg                                                                            <  2:3500332/148‑1 (MQ=255)
                                                                    gccgccATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGtt                                                                          >  2:83191/1‑148 (MQ=255)
                                                                     ccgccATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCTATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTa                                                                         <  1:110989/148‑1 (MQ=255)
                                                                      cgccATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAg                                                                        <  1:3271788/148‑1 (MQ=255)
                                                                         cATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCgg                                                                     <  2:2589243/148‑1 (MQ=255)
                                                                         cATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCgg                                                                     <  2:238026/148‑1 (MQ=255)
                                                                          aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGt                                                                    >  2:1001550/1‑148 (MQ=255)
                                                                          aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGt                                                                    >  2:2632980/1‑148 (MQ=255)
                                                                           tCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTc                                                                   >  1:1273786/1‑148 (MQ=255)
                                                                                gacgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcgacga                                                              <  1:2622985/148‑1 (MQ=255)
                                                                                 acgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAt                                                             <  2:1285220/148‑1 (MQ=255)
                                                                                  cgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGACGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAtc                                                            >  1:1883267/1‑148 (MQ=255)
                                                                                       tCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGATAGCGGTCGACGATCTCCAg                                                       >  1:2996141/1‑148 (MQ=255)
                                                                                              ttAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCgg                                                >  1:948713/1‑148 (MQ=255)
                                                                                              ttAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCgg                                                >  2:1598126/1‑148 (MQ=255)
                                                                                               tAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGa                                               >  1:2028521/1‑148 (MQ=255)
                                                                                               tAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGa                                               >  2:537890/1‑148 (MQ=255)
                                                                                                 gCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATc                                             >  1:1798034/1‑148 (MQ=255)
                                                                                                    gcgcTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGt                                          <  1:3548461/148‑1 (MQ=255)
                                                                                                     cgcTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTAGCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtg                                         <  2:3407775/148‑1 (MQ=255)
                                                                                                        ttcattcaGACCGCTGACGACCTGGCGATGCTGTGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgatg                                      <  1:3386642/148‑1 (MQ=255)
                                                                                                        ttcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgatg                                      >  2:2011585/1‑148 (MQ=255)
                                                                                                        ttcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgatg                                      <  2:2985481/148‑1 (MQ=255)
                                                                                                        ttcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgatg                                      <  2:381405/148‑1 (MQ=255)
                                                                                                        ttcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgatg                                      <  2:2322685/148‑1 (MQ=255)
                                                                                                               aGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGTTGACGCCtt                               >  1:24991/1‑148 (MQ=255)
                                                                                                               aGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCtt                               <  2:733301/148‑1 (MQ=255)
                                                                                                               aGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGGGCGGCTCGGTGCTTACGCCtt                               >  1:25532/1‑148 (MQ=255)
                                                                                                                     cTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGcc                         <  1:499809/148‑1 (MQ=255)
                                                                                                                     cTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGcc                         <  1:1997015/148‑1 (MQ=255)
                                                                                                                       gacgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCa                       <  1:2061514/148‑1 (MQ=255)
                                                                                                                        acgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCAt                      >  2:2434460/1‑148 (MQ=255)
                                                                                                                            ccTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCg                  <  2:4423/148‑1 (MQ=255)
                                                                                                                            ccTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCg                  <  2:1574825/148‑1 (MQ=255)
                                                                                                                            ccTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCCGTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCg                  >  2:69248/1‑148 (MQ=255)
                                                                                                                               ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACa               >  2:267647/1‑148 (MQ=255)
                                                                                                                               ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACa               <  2:2880359/148‑1 (MQ=255)
                                                                                                                                gCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg              >  2:288252/1‑148 (MQ=255)
                                                                                                                                gCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg              >  2:2162313/1‑148 (MQ=255)
                                                                                                                                  gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGc            <  1:3392094/148‑1 (MQ=255)
                                                                                                                                   aTGCAGGGCCTCTATCGCGCTCGATGCCCAAATTTTATGTGCGGAAGGGGCGATTTTTACCAGCAATCGCAACGTATCTTCCGTTCTCGGGAGAAGCTCTCCAGTGAGCGGAGCGGTGATGACGCCTTCAAGCCCCTAGCGACAgggc           >  2:1254697/1‑146 (MQ=255)
                                                                                                                                   aTGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGcc           >  1:395174/1‑148 (MQ=255)
                                                                                                                                   aTGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGcc           >  2:2693094/1‑148 (MQ=255)
                                                                                                                                     gCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTg         >  1:1654852/1‑148 (MQ=255)
                                                                                                                                        gggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaa      <  2:1856151/148‑1 (MQ=255)
                                                                                                                                         ggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaaa     >  2:1957958/1‑148 (MQ=255)
                                                                                                                                          gCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAAc    >  1:932418/1‑148 (MQ=255)
                                                                                                                                            ctctATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACgg  <  1:2911204/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGG  >  CP009273/601704‑601991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑