Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 666,998 G→T 6.1% L22I (CTT→ATT)  holA ← DNA polymerase III, delta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273666,9980GT 6.1% 756.4 / 15.8 202L22I (CTT→ATT) holADNA polymerase III, delta subunit
Reads supporting (aligned to +/- strand):  ref base G (114/75);  new base T (7/6);  total (121/81)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666851‑667144
                                                                                                                                                   |                                                                                                                                                  
tAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAg                                                                                                                                                    <  2:1341195/148‑1 (MQ=255)
tAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAg                                                                                                                                                    <  1:1704449/148‑1 (MQ=255)
     tGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcg                                                                                                                                               <  1:1154217/148‑1 (MQ=255)
      ggCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgc                                                                                                                                              <  2:656418/148‑1 (MQ=255)
          tAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcg                                                                                                                                          >  1:1940839/1‑148 (MQ=255)
          tAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcg                                                                                                                                          >  1:1893258/1‑148 (MQ=255)
              gAACAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCa                                                                                                                                      <  2:3069433/148‑1 (MQ=255)
                aaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGc                                                                                                                                    >  1:924381/1‑148 (MQ=255)
                 aaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCgccgcc                                                                                                                                   >  1:147764/1‑148 (MQ=255)
                  aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGccc                                                                                                                                  <  1:3363958/148‑1 (MQ=255)
                  aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGccc                                                                                                                                  <  1:2648615/148‑1 (MQ=255)
                   gATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCt                                                                                                                                 >  2:1547284/1‑148 (MQ=255)
                   gATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCt                                                                                                                                 >  2:506078/1‑148 (MQ=255)
                    aTCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCtt                                                                                                                                >  1:1443617/1‑148 (MQ=255)
                     tCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTc                                                                                                                               >  2:3394026/1‑148 (MQ=255)
                     tCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTc                                                                                                                               >  2:3544815/1‑148 (MQ=255)
                      cGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCa                                                                                                                              <  2:1526201/148‑1 (MQ=255)
                        cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt                                                                                                                            >  1:76709/1‑148 (MQ=255)
                          ttCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGa                                                                                                                          >  1:2935202/1‑148 (MQ=255)
                          ttCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGa                                                                                                                          >  1:563504/1‑148 (MQ=255)
                           tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAg                                                                                                                         >  1:2927252/1‑148 (MQ=255)
                           tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAg                                                                                                                         <  1:3277780/148‑1 (MQ=255)
                                    tgtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGcgcgcg                                                                                                                >  2:1181723/1‑148 (MQ=255)
                                     gtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg                                                                                                               >  1:992945/1‑148 (MQ=255)
                                     gtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg                                                                                                               >  1:2630464/1‑148 (MQ=255)
                                     gtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATGGAGCTGCGCGCgg                                                                                                               >  1:843526/1‑148 (MQ=255)
                                      ttGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGa                                                                                                              >  1:664647/1‑148 (MQ=255)
                                       tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAg                                                                                                             <  1:245627/148‑1 (MQ=255)
                                        gggATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgt                                                                                                            >  1:2660066/1‑148 (MQ=255)
                                           aTCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgt                                                                                                         <  2:1325199/148‑1 (MQ=255)
                                           aTCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgt                                                                                                         <  2:1325170/148‑1 (MQ=255)
                                            tCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgtt                                                                                                        <  1:1163811/148‑1 (MQ=255)
                                             cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTc                                                                                                       >  2:2176680/1‑148 (MQ=255)
                                             cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCGGCGCGCGGAGTTGTTc                                                                                                       >  1:2571095/1‑148 (MQ=255)
                                             cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGACCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGAAACGCCGCGCGCAGCCCATCATAGAGCACCGCGCGGAgttgtta                                                                                                       >  1:2899272/1‑147 (MQ=255)
                                               aTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCg                                                                                                     >  1:588774/1‑148 (MQ=255)
                                                  gAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCACAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGt                                                                                                  >  2:1429117/1‑148 (MQ=255)
                                                      aaGGGTGGTGTTCTTCGAATCCGTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCCAGTAAAAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGTACaa                                                                                              <  2:2969082/148‑1 (MQ=255)
                                                       aGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAc                                                                                             >  1:1841720/1‑148 (MQ=255)
                                                          gtggtgTTCTTCGACTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTGCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGa                                                                                          <  2:3128503/148‑1 (MQ=255)
                                                          gtggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGa                                                                                          >  1:3048500/1‑148 (MQ=255)
                                                            ggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAat                                                                                        >  2:209498/1‑148 (MQ=255)
                                                             gtgtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGTGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAata                                                                                       >  1:3068082/1‑147 (MQ=255)
                                                                ttcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcatc                                                                                    >  2:1551635/1‑148 (MQ=255)
                                                                ttcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcatc                                                                                    <  2:779639/148‑1 (MQ=255)
                                                                 tcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAtcatca                                                                                   <  1:451398/148‑1 (MQ=255)
                                                                      gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAc                                                                              >  2:1821169/1‑148 (MQ=255)
                                                                      gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAc                                                                              <  2:1275378/148‑1 (MQ=255)
                                                                       aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAcc                                                                             <  2:850851/148‑1 (MQ=255)
                                                                        aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCAACAGTTAccc                                                                            >  1:1411021/1‑148 (MQ=255)
                                                                          ccTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGGGCCGCCCGTCAGTGTGCTGCGCGCGGAGTTGTTGCGGGGACCACCCAAAAATCAGTTAACCac                                                                          >  1:2408344/1‑147 (MQ=255)
                                                                          ccTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                          >  2:1720558/1‑148 (MQ=255)
                                                                          ccTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                          >  1:501776/1‑148 (MQ=255)
                                                                            ttGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCg                                                                        >  1:2318519/1‑148 (MQ=255)
                                                                             tgtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgt                                                                       >  2:1243449/1‑148 (MQ=255)
                                                                               tgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtgg                                                                     <  1:3334536/148‑1 (MQ=255)
                                                                                 ccGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCATCATTGAGCTGCGCGCGGAGTTGTTGCGGGTACAACCGAATCATCAGTTACCCAGCgtggtg                                                                   >  2:903250/1‑148 (MQ=255)
                                                                                    cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGgag                                                                <  1:2176680/148‑1 (MQ=255)
                                                                                    cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGgag                                                                <  1:2244473/148‑1 (MQ=255)
                                                                                         aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAcgcg                                                           <  2:1680555/148‑1 (MQ=255)
                                                                                         aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAcgcg                                                           <  2:2010862/148‑1 (MQ=255)
                                                                                         aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAcgcg                                                           <  2:324167/148‑1 (MQ=255)
                                                                                          ccTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGGGCCGCCCGTCATTGAGCTGGGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGGGGAGACGCGt                                                          >  1:174735/1‑148 (MQ=255)
                                                                                          ccTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCGGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCACCGTGGTGGAGACGCGt                                                          >  1:174601/1‑148 (MQ=255)
                                                                                               cGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAgg                                                     <  1:1815341/148‑1 (MQ=255)
                                                                                                 aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTACAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGGTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCg                                                   <  2:1940839/148‑1 (MQ=255)
                                                                                                 aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCg                                                   <  2:1893258/148‑1 (MQ=255)
                                                                                                 aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCg                                                   <  1:2372201/148‑1 (MQ=255)
                                                                                                    aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTg                                                >  2:2685740/1‑148 (MQ=255)
                                                                                                    aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTg                                                >  2:892788/1‑148 (MQ=255)
                                                                                                    aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCGGCGCGCGGAGTTGTTCCGGGTTCAACCGAATCATCAGTTACCCCGCGGGGTGGAGACGCGTGCAGGCGTTg                                                >  1:2250680/1‑148 (MQ=255)
                                                                                                    aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCGGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTg                                                >  2:2408180/1‑148 (MQ=255)
                                                                                                          cTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAg                                          <  2:1724695/148‑1 (MQ=255)
                                                                                                           tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTGATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGgtgt                                         >  2:289994/1‑148 (MQ=255)
                                                                                                           tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGt                                         >  2:289801/1‑148 (MQ=255)
                                                                                                           tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGt                                         >  2:1361656/1‑148 (MQ=255)
                                                                                                            ggCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCCGCCCGTCAGGGAGCTGCGCGCGGGGTTGTTACGGGTGCAACCGCATCATCAATTTCCCAGCGGGGTGGGGACGCGTGCAGGCGGTGGCGgtgtt                                        >  1:36946/1‑147 (MQ=255)
                                                                                                            ggCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGGCAATGAGCTGCGCGCGGAGGTGGTGCGGGTACAACCCCAACAACACTTACCCACCGTGGGGGTGACGCGTGCCGGCGGTGCCGGAGTa                                        >  1:2418355/1‑148 (MQ=255)
                                                                                                             gCTTTCCTGCAATCACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTAt                                       <  2:3334022/148‑1 (MQ=255)
                                                                                                                 tCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGt                                   <  1:1011469/148‑1 (MQ=255)
                                                                                                                 tCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGt                                   <  2:1162178/148‑1 (MQ=255)
                                                                                                                      cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTgg                              >  2:2588452/1‑148 (MQ=255)
                                                                                                                         tAACAGAGTATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCg                           >  2:279235/1‑148 (MQ=255)
                                                                                                                         tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCg                           <  1:3001425/148‑1 (MQ=255)
                                                                                                                         tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTTAGCTGCGCGCGGAGTTGTTCCGGGTACAACCCAAACATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCg                           >  1:1414381/1‑148 (MQ=255)
                                                                                                                         tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTGCGGGGACAACCCAATAATCAGTTAACCACCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATACGTTGTGGtgg                           >  1:3452410/1‑146 (MQ=255)
                                                                                                                          aaCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCGGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGGGACGCGTGCAGGCGTTGCCGGAGTATACGGTGTGGTCGa                          >  1:169876/1‑148 (MQ=255)
                                                                                                                           aCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTGCGGGTACAACCGAAACATCAGTTAACCAGCGTGGTGGAGACGCGTGCAGGCGGTGCCGGAGTATCCGTTGTGGTCGAc                         >  1:426157/1‑148 (MQ=255)
                                                                                                                           aCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAc                         <  1:1604226/148‑1 (MQ=255)
                                                                                                                            cAGAGGATCGTTACCAAGTACAAGATACGCCGCGCGCAGCCCTTCCTTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACg                        <  2:3483045/148‑1 (MQ=255)
                                                                                                                            cAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCATTTATCGGCGCGCGGCGTTGTTCCGGGTACAACCCAATCAACAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGGTGCCGGAGTATCCGTTGGGGTGGACg                        >  1:3524222/1‑148 (MQ=255)
                                                                                                                            cAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGGTCCGGGTACAACCGAATCATAAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGGTGTGGTCGACg                        >  2:1630945/1‑148 (MQ=255)
                                                                                                                             agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGt                       <  2:1018359/148‑1 (MQ=255)
                                                                                                                               agGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGTGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt                     >  1:2097578/1‑148 (MQ=255)
                                                                                                                               agGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt                     <  1:1669274/148‑1 (MQ=255)
                                                                                                                                 gATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGt                   <  1:1720558/148‑1 (MQ=255)
                                                                                                                                    cGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTTAGCTGCGCGCGGAGTTTTTACGGGTACAACCCAAACATCAGTTACCCCGCGGGGTGGAGACGCGTGCAGGCGTTGCCCGAGTATCCGTTGTGGTCGACGTCTGttct                >  1:2555068/1‑148 (MQ=255)
                                                                                                                                     gTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttctt               <  2:3285004/148‑1 (MQ=255)
                                                                                                                                            aGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGa        >  2:1123667/1‑148 (MQ=255)
                                                                                                                                                aaaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGa    <  2:2652329/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666851‑667144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑