Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,899 C→A 100% D781Y (GAT→TAT)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,8990CA99.0% 643.5 / ‑1.6 199D781Y (GAT→TAT) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (81/116);  total (83/116)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043
                                                                                                                                                 |                                                                                                                                                
 cACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcc                                                                                                                                               >  1:1748562/1‑148 (MQ=255)
  aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcct                                                                                                                                              <  2:2981889/148‑1 (MQ=255)
   cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                             >  2:2440524/1‑148 (MQ=255)
      tCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcgc                                                                                                                                          >  2:1580244/1‑148 (MQ=255)
           aaCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTc                                                                                                                                     >  1:1861871/1‑148 (MQ=255)
            aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                    >  2:2919598/1‑148 (MQ=255)
              cTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGt                                                                                                                                  >  1:2711231/1‑148 (MQ=255)
               tGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGtt                                                                                                                                 >  2:261108/1‑148 (MQ=255)
                gTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTc                                                                                                                                <  2:2659493/148‑1 (MQ=255)
                 ttcttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTcc                                                                                                                               >  1:489038/1‑148 (MQ=255)
                     tcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTAGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGcc                                                                                                                           >  1:2700724/1‑148 (MQ=255)
                         ttGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                                       <  2:2650998/148‑1 (MQ=255)
                         ttGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                                       <  2:2650808/148‑1 (MQ=255)
                          tGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGc                                                                                                                      >  1:1484161/1‑148 (MQ=255)
                           gCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCg                                                                                                                     >  2:2762962/1‑148 (MQ=255)
                           gCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCg                                                                                                                     <  2:1939962/148‑1 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               >  2:1533468/1‑148 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               <  1:1104706/148‑1 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               <  1:3419580/148‑1 (MQ=255)
                                  ccGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                              >  1:3224314/1‑148 (MQ=255)
                                  ccGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                              >  1:1220280/1‑148 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:3167300/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:219658/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:1257992/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  2:3312573/148‑1 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  2:2459729/1‑148 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  1:2022408/1‑148 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  2:1228255/1‑148 (MQ=255)
                                        cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                        >  1:2117056/1‑148 (MQ=255)
                                          gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                                      <  2:3122466/148‑1 (MQ=255)
                                          gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                                      <  2:1349116/148‑1 (MQ=255)
                                           tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                                     <  2:252765/148‑1 (MQ=255)
                                           tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                                     <  1:872940/148‑1 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  1:1564310/1‑148 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  2:2043412/1‑148 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  2:1722373/1‑148 (MQ=255)
                                              ttttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                  <  1:2752431/148‑1 (MQ=255)
                                              ttttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                  >  1:138645/1‑148 (MQ=255)
                                                ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGa                                                                                                <  2:37368/148‑1 (MQ=255)
                                                ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGa                                                                                                <  2:2341118/148‑1 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  2:3440256/1‑148 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  1:2387260/1‑148 (MQ=255)
                                                     aCGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACggg                                                                                           <  2:909174/148‑1 (MQ=255)
                                                      cGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGt                                                                                          <  1:1363397/148‑1 (MQ=255)
                                                          cTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                                      <  2:1572117/148‑1 (MQ=255)
                                                           tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                     <  2:489038/148‑1 (MQ=255)
                                                           tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                     <  1:2575349/148‑1 (MQ=255)
                                                            ttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCa                                                                                    >  1:2509550/1‑148 (MQ=255)
                                                              aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATa                                                                                  >  2:2809566/1‑148 (MQ=255)
                                                                cGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACg                                                                                >  1:3074661/1‑148 (MQ=255)
                                                                 gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACgg                                                                               <  1:1891275/148‑1 (MQ=255)
                                                                 gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACgg                                                                               <  1:2547920/148‑1 (MQ=255)
                                                                    aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACa                                                                            <  1:3270863/148‑1 (MQ=255)
                                                                     aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACaa                                                                           >  1:3324663/1‑148 (MQ=255)
                                                                     aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACaa                                                                           <  2:1416556/148‑1 (MQ=255)
                                                                      ccTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATTCGGACAAc                                                                          >  1:1530949/1‑148 (MQ=255)
                                                                        tGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACgg                                                                        >  2:827680/1‑148 (MQ=255)
                                                                        tGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACgg                                                                        >  1:709841/1‑148 (MQ=255)
                                                                         gCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGc                                                                       <  2:32278/148‑1 (MQ=255)
                                                                                  ccAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGc                                                              <  1:1580246/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  1:540142/148‑1 (MQ=255)
                                                                                      cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTcc                                                          <  1:1638081/148‑1 (MQ=255)
                                                                                      cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTcc                                                          >  1:1081267/1‑148 (MQ=255)
                                                                                          gAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                      >  1:909483/1‑148 (MQ=255)
                                                                                          gAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                      >  2:1554540/1‑148 (MQ=255)
                                                                                             tCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                                   >  1:2776698/1‑148 (MQ=255)
                                                                                              cTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCag                                                  >  2:169240/1‑148 (MQ=255)
                                                                                                tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCagag                                                <  1:753933/148‑1 (MQ=255)
                                                                                                    ccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGcgcg                                            >  2:400027/1‑148 (MQ=255)
                                                                                                     caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCAACAGAGCGCGa                                           >  2:1615849/1‑148 (MQ=255)
                                                                                                      aTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                          <  2:2143424/148‑1 (MQ=255)
                                                                                                      aTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                          <  2:3148748/148‑1 (MQ=255)
                                                                                                       tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                         <  2:3321481/148‑1 (MQ=255)
                                                                                                       tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                         <  2:7640/148‑1 (MQ=255)
                                                                                                        cGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATcc                                        <  2:3100332/148‑1 (MQ=255)
                                                                                                           tttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGc                                     <  2:1372212/148‑1 (MQ=255)
                                                                                                            ttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCt                                    <  1:3503033/148‑1 (MQ=255)
                                                                                                                  tCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAt                              <  1:2962450/148‑1 (MQ=255)
                                                                                                                   cAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATc                             >  1:580054/1‑148 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGNGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           <  1:1805435/148‑1 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           <  1:688142/148‑1 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           >  2:530903/1‑148 (MQ=255)
                                                                                                                        aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTg                        >  1:2046390/1‑148 (MQ=255)
                                                                                                                        aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTg                        <  2:3244217/148‑1 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:2363644/1‑148 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:974385/1‑148 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:2927980/1‑148 (MQ=255)
                                                                                                                           ggCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg                     >  1:2621798/1‑148 (MQ=255)
                                                                                                                           ggCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg                     <  2:1748562/148‑1 (MQ=255)
                                                                                                                              cACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCtt                  <  2:2483596/148‑1 (MQ=255)
                                                                                                                              cACGGCGCGTTGTCGATAGAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCtt                  <  2:2485406/148‑1 (MQ=255)
                                                                                                                                  gcgcGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGc              >  1:2982755/1‑148 (MQ=255)
                                                                                                                                   cgcgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGcc             >  1:2232141/1‑148 (MQ=255)
                                                                                                                                    gcgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTCTCGCCa            >  1:1161840/1‑148 (MQ=255)
                                                                                                                                     cgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAg           >  2:2570822/1‑148 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  1:1101570/148‑1 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  2:2392779/148‑1 (MQ=255)
                                                                                                                                       ttGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGtt         <  1:1822119/148‑1 (MQ=255)
                                                                                                                                         gTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTg       <  1:2424064/148‑1 (MQ=255)
                                                                                                                                         gTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTg       <  2:1028045/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑