Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 705,588 C→A 100% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273705,5880CA99.5% 663.4 / ‑4.3 206intergenic (+16/+68)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (94/111);  total (95/111)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

GCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705444‑705732
                                                                                                                                                |                                                                                                                                                
gCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCg                                                                                                                                               <  2:2289154/148‑1 (MQ=255)
gCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCg                                                                                                                                               >  1:203221/1‑148 (MQ=255)
 ccGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGc                                                                                                                                              >  1:2576551/1‑148 (MQ=255)
 ccGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGc                                                                                                                                              <  1:602872/148‑1 (MQ=255)
 ccGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGc                                                                                                                                              <  2:2472565/148‑1 (MQ=255)
   gACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCtt                                                                                                                                            <  2:191404/148‑1 (MQ=255)
   gACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCtt                                                                                                                                            <  1:2399365/148‑1 (MQ=255)
      cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTc                                                                                                                                         <  2:676809/148‑1 (MQ=255)
      cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTc                                                                                                                                         <  1:1451894/148‑1 (MQ=255)
       aGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCt                                                                                                                                        <  1:3407554/148‑1 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       <  1:1103163/148‑1 (MQ=255)
         gAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                      <  2:3070720/148‑1 (MQ=255)
         gAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                      <  1:2465957/148‑1 (MQ=255)
          aaaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTAt                                                                                                                                     <  1:1846113/148‑1 (MQ=255)
           aaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTAtt                                                                                                                                    >  1:938642/1‑148 (MQ=255)
            aGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTAttt                                                                                                                                   >  2:1538658/1‑148 (MQ=255)
               gtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTccc                                                                                                                                <  1:3531357/148‑1 (MQ=255)
                tACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCt                                                                                                                               <  1:740016/148‑1 (MQ=255)
                 aCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCTGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTTTCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCtt                                                                                                                              <  1:2132132/148‑1 (MQ=255)
                  cgcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTg                                                                                                                             <  2:2256338/148‑1 (MQ=255)
                         tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaa                                                                                                                      >  2:1986681/1‑148 (MQ=255)
                         tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaa                                                                                                                      <  2:826627/148‑1 (MQ=255)
                          ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaaa                                                                                                                     >  1:527163/1‑148 (MQ=255)
                          ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaaa                                                                                                                     <  1:3025013/148‑1 (MQ=255)
                           gATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaaaa                                                                                                                    >  1:92259/1‑148 (MQ=255)
                                 cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                                                                                                              >  2:2625340/1‑148 (MQ=255)
                                     agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGc                                                                                                          <  2:2094942/148‑1 (MQ=255)
                                        gtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                                                                                                       <  2:630910/148‑1 (MQ=255)
                                          cTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGAGTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                                     >  2:2313871/1‑148 (MQ=255)
                                            gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                                   <  2:3115711/148‑1 (MQ=255)
                                                tcAACTCAAACCTGAAATGATCTGGGGGTTAAAGCCGATTGTTATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCttt                                                                                               <  1:2528223/147‑1 (MQ=255)
                                                  aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                             <  2:1161130/148‑1 (MQ=255)
                                                      cAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                         <  1:228505/148‑1 (MQ=255)
                                                        aaCCGTAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATACGTGAGAGCTGTAACTATCGCTTTTATTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTc                                                                                       <  1:605762/148‑1 (MQ=255)
                                                        aaCCGGACATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATCAGTCAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCTCAGGTTGGCTTTTCTCGTTc                                                                                       <  2:1619703/148‑1 (MQ=255)
                                                         aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGttaa                                                                                      >  2:1822876/1‑146 (MQ=255)
                                                           cGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgg                                                                                    <  2:2211316/148‑1 (MQ=255)
                                                           cGGAAATGATCAGGCGGTGAAAGCAGATTGTGATAAAGTCTGGCAGGAAATCCGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgg                                                                                    <  2:1967604/148‑1 (MQ=255)
                                                             gAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggct                                                                                  >  2:144024/1‑148 (MQ=255)
                                                                 tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                              >  2:145829/1‑148 (MQ=255)
                                                                  gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCtt                                                                             <  1:1671642/148‑1 (MQ=255)
                                                                       ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                        >  2:1747191/1‑148 (MQ=255)
                                                                       ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                        >  2:1065680/1‑148 (MQ=255)
                                                                        gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                       >  2:3553049/1‑148 (MQ=255)
                                                                            tGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc                                                                   >  1:2005472/1‑148 (MQ=255)
                                                                              aaaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                 >  2:2365929/1‑148 (MQ=255)
                                                                              aaaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                 >  2:116255/1‑148 (MQ=255)
                                                                               aaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTg                                                                <  2:92259/148‑1 (MQ=255)
                                                                                aGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAATTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                               >  2:2151419/1‑148 (MQ=255)
                                                                                aGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                               >  2:1319566/1‑148 (MQ=255)
                                                                                  ccGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                             >  1:2962982/1‑148 (MQ=255)
                                                                                    gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCAt                                                           >  2:2432239/1‑148 (MQ=255)
                                                                                     aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATg                                                          <  2:830242/148‑1 (MQ=255)
                                                                                       tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                        <  1:1480563/148‑1 (MQ=255)
                                                                                         tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATACGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                      <  1:3233486/148‑1 (MQ=255)
                                                                                         tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                      >  2:1937848/1‑148 (MQ=255)
                                                                                         tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                      <  2:465271/148‑1 (MQ=255)
                                                                                            tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCt                                                   <  2:217391/148‑1 (MQ=255)
                                                                                            tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCt                                                   >  1:32395/1‑148 (MQ=255)
                                                                                             aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                  >  2:2723790/1‑148 (MQ=255)
                                                                                              aaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTc                                                 >  2:800732/1‑148 (MQ=255)
                                                                                              aaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTc                                                 >  2:2576727/1‑148 (MQ=255)
                                                                                                 tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                              >  1:403603/1‑148 (MQ=255)
                                                                                                     gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          >  1:1283277/1‑148 (MQ=255)
                                                                                                     gCAGGAAATACGCAGGAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          <  2:1918812/148‑1 (MQ=255)
                                                                                                      cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAt                                         <  2:61397/148‑1 (MQ=255)
                                                                                                      cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAt                                         <  2:933440/148‑1 (MQ=255)
                                                                                                        ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                       >  1:142914/1‑148 (MQ=255)
                                                                                                        ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                       >  1:9271/1‑148 (MQ=255)
                                                                                                         gAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      <  1:773612/148‑1 (MQ=255)
                                                                                                         gAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      <  2:298801/148‑1 (MQ=255)
                                                                                                          aaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCACATGTTCATCTTCGCGGCAATCGcc                                     <  1:2997852/148‑1 (MQ=255)
                                                                                                           aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCt                                    <  2:1131628/148‑1 (MQ=255)
                                                                                                                   gTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                            <  2:3181092/148‑1 (MQ=255)
                                                                                                                      ataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                         <  1:2918866/148‑1 (MQ=255)
                                                                                                                       taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        <  1:1066363/148‑1 (MQ=255)
                                                                                                                       taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        >  1:3147516/1‑148 (MQ=255)
                                                                                                                        aaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                       >  1:1580371/1‑148 (MQ=255)
                                                                                                                         aCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc                      >  1:1411980/1‑148 (MQ=255)
                                                                                                                         aCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc                      >  2:2066386/1‑148 (MQ=255)
                                                                                                                             aTAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaa                  <  1:1986142/148‑1 (MQ=255)
                                                                                                                              tAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa                 >  1:2454527/1‑148 (MQ=255)
                                                                                                                               aaGGGAGAGCTGTAACTATCGCTTTTCTTAGTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg                <  2:2007564/148‑1 (MQ=255)
                                                                                                                                aGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa               <  2:1112915/148‑1 (MQ=255)
                                                                                                                                 gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              <  2:2576369/148‑1 (MQ=255)
                                                                                                                                 gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              <  2:3337628/148‑1 (MQ=255)
                                                                                                                                  tGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa             <  1:2616874/148‑1 (MQ=255)
                                                                                                                                  tGAGAGCTGTAACTATCGCTTATCTTATTTCCCTTGCATAAAAAAGCCAACCCGCACGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTCACCGTAAAGATa             >  2:54777/1‑148 (MQ=255)
                                                                                                                                   gagaGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag            >  2:3059137/1‑148 (MQ=255)
                                                                                                                                   gagaGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag            <  1:1198255/148‑1 (MQ=255)
                                                                                                                                    agagCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGaaata           >  2:3230134/1‑144 (MQ=255)
                                                                                                                                    agagCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATaga           >  1:2352111/1‑148 (MQ=255)
                                                                                                                                      agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         >  2:3260206/1‑148 (MQ=255)
                                                                                                                                      agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         >  2:1490129/1‑148 (MQ=255)
                                                                                                                                      agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         >  1:1051972/1‑148 (MQ=255)
                                                                                                                                       gCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGAc        <  2:1915417/148‑1 (MQ=255)
                                                                                                                                         tGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtg      <  1:851273/148‑1 (MQ=255)
                                                                                                                                          gTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgt     >  2:1563410/1‑148 (MQ=255)
                                                                                                                                            aaCTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg   >  1:1027587/1‑148 (MQ=255)
                                                                                                                                            aaCTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg   >  1:2808047/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705444‑705732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑