Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 723,859 G→T 100% V110V (GTC→GTA)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273723,8590GT96.6% 632.2 / 11.9 206V110V (GTC→GTA) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (104/95);  total (105/101)
Fisher's exact test for biased strand distribution p-value = 6.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAG  >  CP009273/723714‑724002
                                                                                                                                                 |                                                                                                                                               
        gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCATAAAGAAAGTTTTGCAACGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGa                                                                                                                                       <  1:1296401/148‑1 (MQ=255)
           aaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcgg                                                                                                                                    <  1:3128904/148‑1 (MQ=255)
            aCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggc                                                                                                                                   >  2:2428795/1‑148 (MQ=255)
                aCGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTa                                                                                                                               <  2:1988296/148‑1 (MQ=255)
                 ctgATGAGGGAAAAAATCACCGAAATCCCTCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGAAATCTTGCTGATATAGCTCAACGTGGTTTCAACGCTATATTATTGCCAGTTTGTATTGGTTACAAAGCTGACGGCGGTAt                                                                                                                              <  2:547972/146‑1 (MQ=255)
                 cGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTAt                                                                                                                              >  1:3073303/1‑148 (MQ=255)
                  ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTAtt                                                                                                                             <  1:155518/148‑1 (MQ=255)
                    aTGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCa                                                                                                                           <  2:2860491/148‑1 (MQ=255)
                     tGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAg                                                                                                                          <  1:2640487/148‑1 (MQ=255)
                        gggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgcgc                                                                                                                       >  1:2725139/1‑148 (MQ=255)
                         ggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGcc                                                                                                                      >  1:282155/1‑148 (MQ=255)
                            aaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGa                                                                                                                   <  1:378468/148‑1 (MQ=255)
                            aaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGa                                                                                                                   <  1:1195177/148‑1 (MQ=255)
                            aaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGa                                                                                                                   <  1:2869312/148‑1 (MQ=255)
                             aaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGAt                                                                                                                  <  2:390380/148‑1 (MQ=255)
                               aaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATcc                                                                                                                >  1:3001018/1‑148 (MQ=255)
                                  cACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc                                                                                                             >  2:115254/1‑148 (MQ=255)
                                  cACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc                                                                                                             <  1:391113/148‑1 (MQ=255)
                                  cACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCACTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc                                                                                                             >  2:1289353/1‑148 (MQ=255)
                                     cGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGAc                                                                                                          >  1:1978192/1‑148 (MQ=255)
                                     cGCAATCCCGCTGGCGGCAGAAAGAAAGTTGTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGAc                                                                                                          <  1:2204819/148‑1 (MQ=255)
                                      gCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACa                                                                                                         >  2:3170041/1‑148 (MQ=255)
                                       cAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAg                                                                                                        <  1:1396570/148‑1 (MQ=255)
                                       cAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCAAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAg                                                                                                        <  1:1634934/148‑1 (MQ=255)
                                        aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc                                                                                                       >  2:1463848/1‑148 (MQ=255)
                                        aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc                                                                                                       >  2:1087545/1‑148 (MQ=255)
                                         aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                      <  1:1176418/148‑1 (MQ=255)
                                          tCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                     >  1:102136/1‑148 (MQ=255)
                                          tCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                     >  2:2325104/1‑148 (MQ=255)
                                          tCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                     <  2:76163/148‑1 (MQ=255)
                                            cctcTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTg                                                                                                   <  2:3470355/145‑1 (MQ=255)
                                             cGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTgg                                                                                                  >  2:2970893/1‑148 (MQ=255)
                                             cGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTgg                                                                                                  >  2:7173/1‑148 (MQ=255)
                                                  gcggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAAct                                                                                             >  2:3299464/1‑148 (MQ=255)
                                                      cAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgctg                                                                                         >  2:849262/1‑148 (MQ=255)
                                                      cAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgctg                                                                                         >  2:747187/1‑148 (MQ=255)
                                                       agaaagaaAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgt                                                                                        <  1:3167397/148‑1 (MQ=255)
                                                        gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                       >  2:2610115/1‑148 (MQ=255)
                                                        gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                       >  2:1552366/1‑148 (MQ=255)
                                                             aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                  >  2:362527/1‑148 (MQ=255)
                                                             aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                  >  2:360753/1‑148 (MQ=255)
                                                             aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                  >  2:1274902/1‑148 (MQ=255)
                                                             aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                  >  1:3388778/1‑148 (MQ=255)
                                                              aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTaa                                                                                 <  1:3501999/148‑1 (MQ=255)
                                                               agTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                <  2:645205/148‑1 (MQ=255)
                                                               agTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                <  1:1359176/148‑1 (MQ=255)
                                                                 ttttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCg                                                                              >  1:821720/1‑148 (MQ=255)
                                                                  tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                             <  2:2682483/148‑1 (MQ=255)
                                                                   ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                            <  2:1111086/148‑1 (MQ=255)
                                                                     gCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAg                                                                          <  1:106414/148‑1 (MQ=255)
                                                                     gCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAg                                                                          <  1:2128123/148‑1 (MQ=255)
                                                                        ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTTGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCAAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  1:2522304/148‑1 (MQ=255)
                                                                        ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  1:2522398/148‑1 (MQ=255)
                                                                        ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  2:1216253/148‑1 (MQ=255)
                                                                        ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCAACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       >  1:1833738/1‑148 (MQ=255)
                                                                            tAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGc                                                                   >  1:797555/1‑148 (MQ=255)
                                                                             aaGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCt                                                                  >  1:1461546/1‑148 (MQ=255)
                                                                               gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa                                                                >  2:1240979/1‑148 (MQ=255)
                                                                               gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa                                                                <  1:3196211/148‑1 (MQ=255)
                                                                                 ccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAcc                                                              <  2:3357424/148‑1 (MQ=255)
                                                                                 ccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAcc                                                              <  2:3058041/148‑1 (MQ=255)
                                                                                  cGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCg                                                             <  2:1599487/148‑1 (MQ=255)
                                                                                  cGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCg                                                             >  1:2232264/1‑148 (MQ=255)
                                                                                       tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa                                                        <  1:3079371/148‑1 (MQ=255)
                                                                                       tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa                                                        >  1:686810/1‑148 (MQ=255)
                                                                                        cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                       <  2:428462/148‑1 (MQ=255)
                                                                                        cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                       >  1:2427115/1‑148 (MQ=255)
                                                                                         tGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACa                                                      <  2:2222293/148‑1 (MQ=255)
                                                                                                   aGCTCAACGTGGTTTCACCGCTATAAGTTTGCCAGTTGGTTTTTGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAg                                            <  2:103497/148‑1 (MQ=255)
                                                                                                   aGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCACCATAAAAAACAg                                            >  2:2531444/1‑148 (MQ=255)
                                                                                                   aGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAg                                            >  2:285422/1‑148 (MQ=255)
                                                                                                     cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                          <  1:2787766/148‑1 (MQ=255)
                                                                                                     cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                          <  2:292842/148‑1 (MQ=255)
                                                                                                     cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                          <  2:2916579/148‑1 (MQ=255)
                                                                                                     cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                          <  2:1635911/148‑1 (MQ=255)
                                                                                                       cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAcc                                        >  1:3488794/1‑148 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGTTTTTGGTTAAAAAGGTGACGGCGGTATTCAGCGCCAGTTCCCACTACAGCCCTGGCAACTTCTGTGGATTAAGCGGCAGATAGTGCTGACAGTGCAACATAAAAAACAGCACCGcc                                     <  2:663973/148‑1 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                     <  1:1576747/148‑1 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                     >  1:2597453/1‑148 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                     >  1:977248/1‑148 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                     >  1:2959100/1‑148 (MQ=255)
                                                                                                          cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                     >  1:2255749/1‑148 (MQ=255)
                                                                                                              gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                 >  1:360640/1‑148 (MQ=255)
                                                                                                               tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGccc                                >  2:2118357/1‑148 (MQ=255)
                                                                                                               tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGccc                                <  1:2626926/148‑1 (MQ=255)
                                                                                                                 tCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCa                              <  1:143832/148‑1 (MQ=255)
                                                                                                                  cACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAg                             <  2:2418712/148‑1 (MQ=255)
                                                                                                                    ccGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCa                           >  1:553023/1‑148 (MQ=255)
                                                                                                                         ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                      >  1:1974830/1‑148 (MQ=255)
                                                                                                                         ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                      <  1:721181/148‑1 (MQ=255)
                                                                                                                          taAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                     <  1:2329843/148‑1 (MQ=255)
                                                                                                                          taAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                     >  1:2015689/1‑148 (MQ=255)
                                                                                                                                   cAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGaa            <  1:123407/148‑1 (MQ=255)
                                                                                                                                    aGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAAt           >  2:2168938/1‑148 (MQ=255)
                                                                                                                                    aGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAAt           >  2:374359/1‑148 (MQ=255)
                                                                                                                                     gTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATg          >  1:1801121/1‑148 (MQ=255)
                                                                                                                                      ttGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATgg         >  1:2806660/1‑148 (MQ=255)
                                                                                                                                       tGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGc        >  1:2072681/1‑148 (MQ=255)
                                                                                                                                       tGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGc        >  2:280920/1‑148 (MQ=255)
                                                                                                                                             tGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg  <  2:2463451/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAG  >  CP009273/723714‑724002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑