Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 726,087 G→T 100% R350L (CGT→CTT)  rhsC → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273726,0870GT96.2% 550.2 / 12.1 186R350L (CGT→CTT) rhsCRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base T (101/78);  total (103/83)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

TACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAG  >  CP009273/725942‑726230
                                                                                                                                                 |                                                                                                                                               
tACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTc                                                                                                                                               <  2:1068042/148‑1 (MQ=255)
 aCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTca                                                                                                                                              >  1:332024/1‑148 (MQ=255)
      gagaATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACgg                                                                                                                                         >  2:2056097/1‑148 (MQ=255)
           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:2575600/1‑148 (MQ=255)
           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:1085679/1‑148 (MQ=255)
           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:487678/1‑148 (MQ=255)
           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGa                                                                                                                                    <  1:2897714/148‑1 (MQ=255)
            ttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGAc                                                                                                                                   >  1:2977522/1‑148 (MQ=255)
               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:813117/1‑148 (MQ=255)
               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:813105/1‑148 (MQ=255)
               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:3366164/1‑148 (MQ=255)
                   ccgcgccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGAGCCGACCGGAAAt                                                                                                                            <  1:2478800/148‑1 (MQ=255)
                    cgcgccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATc                                                                                                                           <  2:1179219/148‑1 (MQ=255)
                       gccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGt                                                                                                                        <  1:3181966/148‑1 (MQ=255)
                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   >  1:143139/1‑148 (MQ=255)
                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   >  1:142889/1‑148 (MQ=255)
                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  1:622970/148‑1 (MQ=255)
                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  2:2379866/148‑1 (MQ=255)
                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  2:471315/148‑1 (MQ=255)
                             ggTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGt                                                                                                                  >  1:104502/1‑148 (MQ=255)
                             ggTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGt                                                                                                                  >  1:103844/1‑148 (MQ=255)
                              gTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGtt                                                                                                                 <  2:23743/148‑1 (MQ=255)
                              gTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGAGAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGtt                                                                                                                 <  2:264360/148‑1 (MQ=255)
                                gcgcTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTAc                                                                                                               >  1:1839683/1‑148 (MQ=255)
                                       ggCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCg                                                                                                        >  1:1510339/1‑148 (MQ=255)
                                       ggCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCg                                                                                                        <  2:2878166/148‑1 (MQ=255)
                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  2:3237939/148‑1 (MQ=255)
                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  2:3237995/148‑1 (MQ=255)
                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  1:1148186/148‑1 (MQ=255)
                                          tGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcg                                                                                                     <  2:673714/148‑1 (MQ=255)
                                          tGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcg                                                                                                     <  2:127014/148‑1 (MQ=255)
                                           ggACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgg                                                                                                    <  2:1173621/148‑1 (MQ=255)
                                            gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                   >  2:2558489/1‑148 (MQ=255)
                                            gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                   >  2:1675848/1‑148 (MQ=255)
                                             aCGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggc                                                                                                  >  1:221104/1‑148 (MQ=255)
                                               gCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcgg                                                                                                <  2:1522880/148‑1 (MQ=255)
                                                 cccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGt                                                                                              >  2:957104/1‑148 (MQ=255)
                                                  ccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTg                                                                                             <  1:2056018/148‑1 (MQ=255)
                                                  ccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTg                                                                                             <  1:2056097/148‑1 (MQ=255)
                                                   cgcgGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGa                                                                                            <  1:2331122/148‑1 (MQ=255)
                                                        cgAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGaa                                                                                       <  1:2959928/148‑1 (MQ=255)
                                                          aaCTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACa                                                                                     >  1:998120/1‑148 (MQ=255)
                                                            cTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGc                                                                                   >  1:3100935/1‑148 (MQ=255)
                                                               gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                >  2:2741817/1‑148 (MQ=255)
                                                               gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                >  1:1361872/1‑148 (MQ=255)
                                                                  gcggTGTATGACCGCAGCAATACACAGGTGCTCAGATTTACTTACGATGATAAATACGGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAAcc                                                                             <  1:1637901/148‑1 (MQ=255)
                                                                      tgtATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGc                                                                         >  2:3518540/1‑148 (MQ=255)
                                                                       gtATGACCGCAGCAACACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCa                                                                        <  1:844859/148‑1 (MQ=255)
                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGTAGGCtt                                                                   <  2:3108479/148‑1 (MQ=255)
                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGTAGGCtt                                                                   <  2:2824279/148‑1 (MQ=255)
                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCtt                                                                   <  2:451397/148‑1 (MQ=255)
                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCtt                                                                   <  1:1233505/148‑1 (MQ=255)
                                                                                 agcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGtt                                                              <  1:235307/148‑1 (MQ=255)
                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGTCGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  2:142889/148‑1 (MQ=255)
                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  1:1509087/148‑1 (MQ=255)
                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  1:2438026/148‑1 (MQ=255)
                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  2:143139/148‑1 (MQ=255)
                                                                                   caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                            <  1:1192332/148‑1 (MQ=255)
                                                                                   caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                            >  2:2893629/1‑148 (MQ=255)
                                                                                    aaTACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTaca                                                           <  2:813105/148‑1 (MQ=255)
                                                                                     aTACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTacac                                                          >  1:568270/1‑148 (MQ=255)
                                                                                      tACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACg                                                         <  1:381687/148‑1 (MQ=255)
                                                                                       acacAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGt                                                        >  1:878695/1‑148 (MQ=38)
                                                                                       acacAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGt                                                        >  1:2665940/1‑148 (MQ=38)
                                                                                        cacaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTa                                                       >  2:378205/1‑148 (MQ=38)
                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:3408698/1‑148 (MQ=37)
                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:1217294/1‑148 (MQ=37)
                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:1463305/1‑148 (MQ=37)
                                                                                             gTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGt                                                  >  1:3517022/1‑148 (MQ=37)
                                                                                              tGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTa                                                 <  1:2969773/148‑1 (MQ=37)
                                                                                                  cAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgag                                             >  1:173402/1‑148 (MQ=37)
                                                                                                   aGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgaga                                            <  2:1649855/148‑1 (MQ=37)
                                                                                                   aGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgaga                                            <  1:2255266/148‑1 (MQ=37)
                                                                                                       ttacttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGa                                        <  1:2891283/148‑1 (MQ=255)
                                                                                                         acttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGAcc                                      >  1:127498/1‑148 (MQ=255)
                                                                                                           ttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGc                                    >  1:77320/1‑148 (MQ=255)
                                                                                                             acGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcat                                  <  1:284755/148‑1 (MQ=255)
                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:121192/148‑1 (MQ=255)
                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:2054921/148‑1 (MQ=255)
                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:3193194/148‑1 (MQ=255)
                                                                                                                atgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                               >  1:190174/1‑148 (MQ=255)
                                                                                                                atgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                               >  2:2205457/1‑148 (MQ=39)
                                                                                                                 tgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                              >  1:2295384/1‑148 (MQ=255)
                                                                                                                        tACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCg                       <  2:2694439/148‑1 (MQ=39)
                                                                                                                             gggCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGc                  >  1:1466301/1‑148 (MQ=255)
                                                                                                                              ggCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGcc                 >  2:133605/1‑148 (MQ=255)
                                                                                                                               gCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCt                >  2:17051/1‑148 (MQ=255)
                                                                                                                                  ggATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:2789860/1‑148 (MQ=255)
                                                                                                                                  ggATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:2791753/1‑148 (MQ=255)
                                                                                                                                  ggATGGTCGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGATACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:157050/1‑148 (MQ=255)
                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGACGGACCGCATCACCATCACCGACAGCCTGAAc            >  2:2990125/1‑148 (MQ=255)
                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  2:2450389/1‑148 (MQ=255)
                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1055540/1‑148 (MQ=255)
                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1066893/1‑148 (MQ=255)
                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1069736/1‑148 (MQ=255)
                                                                                                                                         ggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGt      >  2:482420/1‑148 (MQ=255)
                                                                                                                                             cACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  >  2:1092733/1‑148 (MQ=255)
                                                                                                                                             cACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  >  2:943004/1‑148 (MQ=255)
                                                                                                                                             cACCGTCACACGGGCCGAGCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  <  2:2392412/148‑1 (MQ=255)
                                                                                                                                             cACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  <  2:3116251/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAG  >  CP009273/725942‑726230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑