Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 759,000 C→A 100% F177L (TTC→TTA)  sucC → succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273759,0000CA99.3% 479.4 / ‑4.1 147F177L (TTC→TTA) sucCsuccinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (73/73);  total (74/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 147 total aligned reads displayed.

GCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTC  >  CP009273/758855‑759141
                                                                                                                                                 |                                                                                                                                             
gcggcgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAc                                                                                                                                             <  1:1374396/148‑1 (MQ=255)
      tGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAAt                                                                                                                                       <  2:1316625/148‑1 (MQ=255)
      tGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAAt                                                                                                                                       <  2:1098853/148‑1 (MQ=255)
      tGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAAt                                                                                                                                       <  2:1316665/148‑1 (MQ=255)
       ggAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATc                                                                                                                                      <  1:1297832/148‑1 (MQ=255)
        gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                     >  2:1912492/1‑148 (MQ=255)
        gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                     >  2:1674770/1‑148 (MQ=255)
        gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                     >  2:3032489/1‑148 (MQ=255)
         aaaTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCtt                                                                                                                                    >  1:3499896/1‑148 (MQ=255)
          taTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCAGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTTAACTGGTTCAGCAGTTAACCAAAATCTTc                                                                                                                                   <  1:242204/147‑1 (MQ=255)
           aTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCa                                                                                                                                  <  2:2094986/148‑1 (MQ=255)
            tCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCAt                                                                                                                                 <  1:3345942/148‑1 (MQ=255)
             cGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATg                                                                                                                                <  2:2966546/148‑1 (MQ=255)
               aaaaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATggg                                                                                                                              >  2:1925805/1‑148 (MQ=255)
               aaaaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATggg                                                                                                                              >  1:804764/1‑148 (MQ=255)
                aaaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGc                                                                                                                             <  2:3131233/148‑1 (MQ=255)
                 aaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGcc                                                                                                                            <  1:132756/148‑1 (MQ=255)
                 aaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGcc                                                                                                                            >  2:1429236/1‑148 (MQ=255)
                   aGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                          <  1:2466038/148‑1 (MQ=255)
                   aGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                          <  1:676730/148‑1 (MQ=255)
                   aGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                          <  1:2465661/148‑1 (MQ=255)
                   aGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                          >  1:2057312/1‑148 (MQ=255)
                        cGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAACTCTTCATGGGCCTGGCGAc                                                                                                                     <  2:1977092/148‑1 (MQ=255)
                           aagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAACAAAATCTTCATGGGCCTGGCGACCAt                                                                                                                  >  1:1844524/1‑148 (MQ=255)
                            agaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGTCGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtt                                                                                                                 <  1:1868394/148‑1 (MQ=255)
                               aaCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTc                                                                                                              <  1:1827151/148‑1 (MQ=255)
                                aCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTcc                                                                                                             >  2:2197953/1‑148 (MQ=255)
                                 cTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCt                                                                                                            >  1:389813/1‑148 (MQ=255)
                                   ccGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTgg                                                                                                          >  1:75746/1‑148 (MQ=255)
                                    cGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGa                                                                                                         >  2:812967/1‑148 (MQ=255)
                                     gCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAg                                                                                                        <  2:2781262/148‑1 (MQ=255)
                                         cTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGcgcg                                                                                                    >  2:106856/1‑148 (MQ=255)
                                          tGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGa                                                                                                   >  1:3566743/1‑148 (MQ=255)
                                           gATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAc                                                                                                  <  1:1742614/148‑1 (MQ=255)
                                            aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                 >  2:1682942/1‑148 (MQ=255)
                                            aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                 >  2:3215173/1‑148 (MQ=255)
                                            aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                 >  2:1441005/1‑148 (MQ=255)
                                                aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGc                                                                                             >  1:2314299/1‑148 (MQ=255)
                                                aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGc                                                                                             >  1:3002030/1‑148 (MQ=255)
                                                aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGc                                                                                             >  1:1388402/1‑148 (MQ=255)
                                                 tAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCg                                                                                            >  2:2828082/1‑148 (MQ=255)
                                                    aGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTg                                                                                         <  2:2179852/148‑1 (MQ=255)
                                                          gcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGaa                                                                                   <  1:2745270/148‑1 (MQ=255)
                                                          gcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGaa                                                                                   <  1:1792451/148‑1 (MQ=255)
                                                           cTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGaaa                                                                                  >  2:1518976/1‑148 (MQ=255)
                                                            ttGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAGCTGGGTCAGCAGTTAACCAAACTCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCgtcct                                                                                 >  1:2631870/1‑144 (MQ=255)
                                                             tGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATc                                                                                >  2:3341426/1‑148 (MQ=255)
                                                              gATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCa                                                                               <  1:1000363/148‑1 (MQ=255)
                                                              gATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCa                                                                               <  1:229374/148‑1 (MQ=255)
                                                               aTCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCaa                                                                              <  2:1137074/148‑1 (MQ=255)
                                                               aTCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCaa                                                                              >  2:3330603/1‑148 (MQ=255)
                                                               aTCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCaa                                                                              <  2:1988458/148‑1 (MQ=255)
                                                                tCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAc                                                                             <  2:3499896/148‑1 (MQ=255)
                                                                 ccGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAcc                                                                            >  2:3308144/1‑148 (MQ=255)
                                                                  cGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAccc                                                                           <  2:2900907/148‑1 (MQ=255)
                                                                   gCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCg                                                                          >  2:1866776/1‑148 (MQ=255)
                                                                            cccGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGtcatc                                                                 <  2:1190377/148‑1 (MQ=255)
                                                                            cccGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGtcatc                                                                 >  1:2803133/1‑148 (MQ=255)
                                                                                     gTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCCAACAg                                                        >  1:1164750/1‑148 (MQ=255)
                                                                                     gTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAg                                                        >  1:1164148/1‑148 (MQ=255)
                                                                                      tATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAgg                                                       >  2:3295691/1‑148 (MQ=255)
                                                                                       aTCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAggg                                                      <  1:2958913/148‑1 (MQ=255)
                                                                                       aTCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAggg                                                      <  1:2455839/148‑1 (MQ=255)
                                                                                           gggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                  <  1:3475587/148‑1 (MQ=255)
                                                                                           gggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                  <  1:2173663/148‑1 (MQ=255)
                                                                                           gggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                  <  1:1912492/148‑1 (MQ=255)
                                                                                            ggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATc                                                 <  1:2158135/148‑1 (MQ=255)
                                                                                               cgcgAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGa                                              <  1:2299524/148‑1 (MQ=255)
                                                                                               cgcgAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGa                                              >  2:2453809/1‑148 (MQ=255)
                                                                                                gcgAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGAt                                             <  1:1058779/148‑1 (MQ=255)
                                                                                                     cTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGcc                                        <  2:2495942/148‑1 (MQ=255)
                                                                                                        gCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCg                                     <  1:2433635/148‑1 (MQ=255)
                                                                                                         cGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGa                                    >  1:2625805/1‑148 (MQ=255)
                                                                                                         cGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGa                                    <  2:3342894/148‑1 (MQ=255)
                                                                                                           ttCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACg                                  <  2:1915913/148‑1 (MQ=255)
                                                                                                           ttCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACg                                  <  1:2047995/148‑1 (MQ=255)
                                                                                                              aaaCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCa                               >  2:953080/1‑148 (MQ=255)
                                                                                                               aaCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCaa                              >  2:2062237/1‑148 (MQ=255)
                                                                                                                aCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCaaa                             <  1:2419360/148‑1 (MQ=255)
                                                                                                                 cTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAAc                            >  1:2196311/1‑148 (MQ=255)
                                                                                                                   gggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCTCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTg                          >  2:2807027/1‑148 (MQ=255)
                                                                                                                   gggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTg                          >  2:652789/1‑148 (MQ=255)
                                                                                                                   gggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTg                          >  1:3328880/1‑148 (MQ=255)
                                                                                                                    ggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTgg                         >  1:3137744/1‑148 (MQ=255)
                                                                                                                    ggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTgg                         >  1:3274359/1‑148 (MQ=255)
                                                                                                                       cTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGgcg                      <  1:2885048/148‑1 (MQ=255)
                                                                                                                        tGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGgcgc                     <  2:2365877/148‑1 (MQ=255)
                                                                                                                        tGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGgcgc                     <  2:2365988/148‑1 (MQ=255)
                                                                                                                          gAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTg                   >  2:1374383/1‑148 (MQ=255)
                                                                                                                           aaGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGa                  >  1:1055409/1‑148 (MQ=255)
                                                                                                                            aGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGAc                 >  2:1929489/1‑148 (MQ=255)
                                                                                                                             ggTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACg                >  2:3334045/1‑148 (MQ=255)
                                                                                                                             ggTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACg                <  1:1795580/148‑1 (MQ=255)
                                                                                                                               tAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGc              <  2:684186/148‑1 (MQ=255)
                                                                                                                                aaaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCa             >  2:2612898/1‑148 (MQ=255)
                                                                                                                                 aaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCaa            <  1:3307207/148‑1 (MQ=255)
                                                                                                                                 aaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCaa            >  1:1536896/1‑148 (MQ=255)
                                                                                                                                     ggTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCa        <  1:144251/148‑1 (MQ=255)
                                                                                                                                         cagcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGt    <  2:2723942/148‑1 (MQ=255)
                                                                                                                                           gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTc  <  1:2828082/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTC  >  CP009273/758855‑759141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑