Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,779 G→T 100% E607D (GAG→GAT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,7790GT98.9% 563.9 / ‑1.8 177E607D (GAG→GAT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (96/79);  total (97/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

TGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGT  >  CP009273/800635‑800923
                                                                                                                                                |                                                                                                                                                
        cttcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGTCTGTCAGCGAGCTGAGAGATGTGTTTgcgcg                                                                                                                                       >  2:1855191/1‑148 (MQ=255)
           cttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAt                                                                                                                                    >  2:1154820/1‑148 (MQ=255)
           cttATCGTTAAATTCCTATTGGTCTGGCGGATTTTACCCTGTCGCGGCGCTATCCCGTTTATGTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAt                                                                                                                                    <  1:2655803/148‑1 (MQ=255)
             tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                  <  2:2151652/148‑1 (MQ=255)
             tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                  >  2:3016950/1‑148 (MQ=255)
               tCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                <  1:1771983/148‑1 (MQ=255)
                cGTTCAAATCGTATTGGTGTGGCGTAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCATAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGc                                                                                                                               <  2:3419766/148‑1 (MQ=255)
                cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGc                                                                                                                               <  2:170167/148‑1 (MQ=255)
                cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGc                                                                                                                               <  2:2365313/148‑1 (MQ=255)
                  ttCAAATCCGATTGGTCTGGCGGATTTTACCCTGTCCCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                             <  2:139839/148‑1 (MQ=255)
                  ttCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                             >  2:2556043/1‑148 (MQ=255)
                  ttCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                             >  1:113513/1‑148 (MQ=255)
                   tCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGa                                                                                                                            <  1:2944622/148‑1 (MQ=255)
                        tCCGTTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                       <  2:2479530/148‑1 (MQ=255)
                         ccGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTg                                                                                                                      >  2:514296/1‑148 (MQ=255)
                            aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTc                                                                                                                   >  2:593228/1‑148 (MQ=255)
                             ttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCa                                                                                                                  >  2:2055676/1‑148 (MQ=255)
                               ggTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAgg                                                                                                                <  2:2222676/148‑1 (MQ=255)
                                gTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGa                                                                                                               >  1:561954/1‑148 (MQ=255)
                                   tGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCa                                                                                                            >  2:1591667/1‑148 (MQ=255)
                                     ggggtgTTCACCCTGTCGCGCCGCGAGCCCGTTTTTTTTCCCATAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                                                                                          <  1:324517/143‑1 (MQ=255)
                                     gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                                                                                          <  1:1497996/148‑1 (MQ=255)
                                     gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATAAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                                                                                          >  1:3192571/1‑148 (MQ=255)
                                      cGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCatat                                                                                                         <  2:3220442/148‑1 (MQ=255)
                                               cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCTGATTGCTGGTCAGGAGCATATTGATCCGCt                                                                                                >  1:839075/1‑148 (MQ=255)
                                                  tGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCa                                                                                             >  1:2326341/1‑148 (MQ=255)
                                                  tGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCa                                                                                             >  1:1383533/1‑148 (MQ=255)
                                                    tctcGCCGCGAGCCCGGTTAGGTTAGCAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                                                                           <  2:2704944/148‑1 (MQ=255)
                                                      tcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACATATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACt                                                                                         >  1:1046054/1‑148 (MQ=255)
                                                          cgcgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaaa                                                                                     <  1:2184734/148‑1 (MQ=255)
                                                           gcgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCGGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAt                                                                                    <  2:2283550/148‑1 (MQ=255)
                                                              aTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTgg                                                                                 >  2:2433199/1‑148 (MQ=255)
                                                               tCCCGTTTATGTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                <  1:3474444/148‑1 (MQ=255)
                                                               tCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                >  1:975636/1‑148 (MQ=255)
                                                               tCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                >  1:2821169/1‑148 (MQ=255)
                                                               gcccGGTTATGTTAGCAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCTGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                <  1:2995402/147‑1 (MQ=255)
                                                                     ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                          <  2:1235925/148‑1 (MQ=255)
                                                                     ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                          >  1:2414071/1‑148 (MQ=255)
                                                                      tATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg                                                                         >  2:261066/1‑148 (MQ=255)
                                                                          ttAGCACAATAAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                     <  1:458274/148‑1 (MQ=255)
                                                                           tAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                    <  1:2657739/148‑1 (MQ=255)
                                                                           tAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                    <  2:1869860/148‑1 (MQ=255)
                                                                            aGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg                                                                   <  1:3557364/148‑1 (MQ=255)
                                                                            aGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg                                                                   <  1:3557400/148‑1 (MQ=255)
                                                                               aGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCgg                                                                >  2:2083884/1‑148 (MQ=255)
                                                                                gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                               >  1:904037/1‑148 (MQ=255)
                                                                                 aaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                              >  1:1430505/1‑148 (MQ=255)
                                                                                 aaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                              >  2:1088807/1‑148 (MQ=255)
                                                                                  aGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGa                                                             >  1:1225496/1‑148 (MQ=255)
                                                                                    tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           >  2:2791576/1‑148 (MQ=255)
                                                                                    tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           <  2:2849203/148‑1 (MQ=255)
                                                                                    tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           >  1:3319717/1‑148 (MQ=255)
                                                                                    tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           >  2:740376/1‑148 (MQ=255)
                                                                                    tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           <  2:1071352/148‑1 (MQ=255)
                                                                                     aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAc                                                          >  1:3181521/1‑148 (MQ=255)
                                                                                              gctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATg                                                 >  1:1913334/1‑148 (MQ=255)
                                                                                               ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATgg                                                <  2:1115927/148‑1 (MQ=255)
                                                                                               ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATgg                                                >  2:1954866/1‑148 (MQ=255)
                                                                                               ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGATTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATgg                                                <  2:1116361/148‑1 (MQ=255)
                                                                                                tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGt                                               <  1:3217386/148‑1 (MQ=255)
                                                                                                 gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGtt                                              <  1:698630/148‑1 (MQ=255)
                                                                                                 gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGtt                                              <  1:698582/148‑1 (MQ=255)
                                                                                                 gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGtt                                              <  1:699059/148‑1 (MQ=255)
                                                                                                    cTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTg                                           >  2:3516974/1‑148 (MQ=255)
                                                                                                        aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                       >  1:2651783/1‑148 (MQ=255)
                                                                                                        aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                       >  1:1346646/1‑148 (MQ=255)
                                                                                                        aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                       >  2:3170070/1‑148 (MQ=255)
                                                                                                        aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                       >  2:3170150/1‑148 (MQ=255)
                                                                                                         aaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGt                                      >  1:183980/1‑148 (MQ=255)
                                                                                                          aaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTg                                     >  2:1771018/1‑148 (MQ=255)
                                                                                                           aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGa                                    >  1:1108336/1‑148 (MQ=255)
                                                                                                           aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGa                                    >  2:908248/1‑148 (MQ=255)
                                                                                                             cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAAc                                  >  2:307910/1‑148 (MQ=255)
                                                                                                               gCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAg                                <  2:585347/148‑1 (MQ=255)
                                                                                                                   cTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAggcgg                            <  1:3016950/148‑1 (MQ=255)
                                                                                                                       cGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAg                        <  1:1444101/148‑1 (MQ=255)
                                                                                                                          ggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                     >  1:1591820/1‑148 (MQ=255)
                                                                                                                          ggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                     >  2:353225/1‑148 (MQ=255)
                                                                                                                           gAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGTTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGc                    >  1:3134337/1‑148 (MQ=255)
                                                                                                                           gAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGc                    >  1:2007097/1‑148 (MQ=255)
                                                                                                                           gAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGc                    <  2:3109533/148‑1 (MQ=255)
                                                                                                                            aaTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGcc                   <  2:425927/148‑1 (MQ=255)
                                                                                                                             aTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCa                  >  1:2574423/1‑148 (MQ=255)
                                                                                                                               gTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGc                >  1:1859097/1‑148 (MQ=255)
                                                                                                                               gTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGc                >  2:1041201/1‑148 (MQ=255)
                                                                                                                                tCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCg               <  1:1023964/148‑1 (MQ=255)
                                                                                                                                 cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt              <  2:1526368/148‑1 (MQ=255)
                                                                                                                                 cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt              <  2:1277978/148‑1 (MQ=255)
                                                                                                                                 cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt              <  2:1526766/148‑1 (MQ=255)
                                                                                                                                    cGAGCTGACAGATGTGTTTGCGCGCATGAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGtgtg           <  1:2742592/148‑1 (MQ=255)
                                                                                                                                     gAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGa          <  1:2931357/148‑1 (MQ=255)
                                                                                                                                      aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAt         >  1:1531642/1‑148 (MQ=255)
                                                                                                                                      aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAt         <  1:500923/148‑1 (MQ=255)
                                                                                                                                      aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAt         <  1:2400488/148‑1 (MQ=255)
                                                                                                                                       gCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt        >  1:1724384/1‑148 (MQ=255)
                                                                                                                                       gCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt        <  2:843600/148‑1 (MQ=255)
                                                                                                                                        cTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg       <  2:2414071/148‑1 (MQ=255)
                                                                                                                                           aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg    >  2:1741949/1‑148 (MQ=255)
                                                                                                                                           aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg    >  2:547019/1‑148 (MQ=255)
                                                                                                                                            cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg   <  1:1332616/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGT  >  CP009273/800635‑800923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑