Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 820,503 C→A 100% T354T (ACG→ACT)  ybhR ← inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273820,5030CA96.9% 585.7 / 7.7 192T354T (ACG→ACT) ybhRinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (84/102);  total (87/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 192 total aligned reads displayed.

CCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTT  >  CP009273/820363‑820647
                                                                                                                                            |                                                                                                                                                
cccGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCg                                                                                                                                           >  2:2980580/1‑148 (MQ=255)
 ccGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGt                                                                                                                                          <  2:1733941/148‑1 (MQ=255)
    cgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTAt                                                                                                                                       <  1:2460066/148‑1 (MQ=255)
          ggAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAg                                                                                                                                 >  1:2513956/1‑148 (MQ=255)
          ggAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAg                                                                                                                                 >  2:2014324/1‑148 (MQ=255)
           gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGt                                                                                                                                >  2:589444/1‑148 (MQ=255)
            aaaaCAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTa                                                                                                                               <  2:3511920/148‑1 (MQ=255)
            aaaaCAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTa                                                                                                                               >  2:3475768/1‑148 (MQ=255)
                caacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                           >  1:573882/1‑148 (MQ=255)
                 aacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGc                                                                                                                          <  1:2708749/148‑1 (MQ=255)
                    tttttGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCcac                                                                                                                       >  2:3179739/1‑148 (MQ=255)
                      tttGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACaa                                                                                                                     <  1:1006231/148‑1 (MQ=255)
                            ggCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGTCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTAtt                                                                                                               <  2:2808067/148‑1 (MQ=255)
                            ggCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAtt                                                                                                               >  1:1890729/1‑148 (MQ=255)
                             gCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTc                                                                                                              <  1:3444630/148‑1 (MQ=255)
                             gCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTc                                                                                                              >  1:1591723/1‑148 (MQ=255)
                             gCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTc                                                                                                              <  2:2042575/148‑1 (MQ=255)
                              cAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTcc                                                                                                             <  2:1747631/148‑1 (MQ=255)
                              cAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTcc                                                                                                             <  1:2203201/148‑1 (MQ=255)
                              cAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTcc                                                                                                             <  2:1982985/148‑1 (MQ=255)
                                aTTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcac                                                                                                           >  1:234186/1‑148 (MQ=255)
                                 ttGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcaca                                                                                                          <  1:2155910/148‑1 (MQ=255)
                                  tGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcacac                                                                                                         >  1:1471700/1‑148 (MQ=255)
                                   gCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcacaca                                                                                                        <  1:197223/148‑1 (MQ=255)
                                    cAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACaa                                                                                                       <  2:2488286/148‑1 (MQ=255)
                                    cAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACaa                                                                                                       <  2:2486933/148‑1 (MQ=255)
                                       ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTAACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                    >  1:3056200/1‑148 (MQ=255)
                                        gCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATc                                                                                                   <  1:2232241/148‑1 (MQ=255)
                                        gCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACACCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATc                                                                                                   >  2:1570578/1‑148 (MQ=255)
                                             cAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACACTATCCAGAc                                                                                              >  1:1199351/1‑148 (MQ=255)
                                              aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACt                                                                                             <  1:1800763/148‑1 (MQ=255)
                                                tCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCg                                                                                           >  1:3493691/1‑148 (MQ=255)
                                                 ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCACTAGCGGCCACAAACTAATACACACAAAATACAGACTccc                                                                                          >  1:3344501/1‑146 (MQ=255)
                                                 ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAACCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAAATATTACACAAAATATCCAGACTAGc                                                                                          >  1:3203894/1‑148 (MQ=255)
                                                  cTTGCCGGCCCGGCGGTAGTGTCTTTGCTGTCGAAAGATAAGAAGTTACATCACCTTACGTATAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCATTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCa                                                                                         <  1:2426671/148‑1 (MQ=255)
                                                    tGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATc                                                                                       >  2:1177329/1‑148 (MQ=255)
                                                     gCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATcc                                                                                      <  1:1294523/148‑1 (MQ=255)
                                                     gCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATcc                                                                                      <  2:1421462/148‑1 (MQ=255)
                                                          cccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaa                                                                                 >  1:3148057/1‑148 (MQ=255)
                                                              gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                             <  1:134798/148‑1 (MQ=255)
                                                               cggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaa                                                                            <  1:934946/148‑1 (MQ=255)
                                                                ggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                           <  1:2137531/148‑1 (MQ=255)
                                                                 gTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATc                                                                          <  2:3108729/148‑1 (MQ=255)
                                                                   aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTg                                                                        <  1:1694034/148‑1 (MQ=255)
                                                                    gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                       <  2:710894/148‑1 (MQ=255)
                                                                    gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                       <  2:1536551/148‑1 (MQ=255)
                                                                       tCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                    <  2:2545677/116‑1 (MQ=255)
                                                                       tCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                    <  2:84432/116‑1 (MQ=255)
                                                                        cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTgg                                                                   <  2:23517/148‑1 (MQ=255)
                                                                         tttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                  >  1:3515727/1‑148 (MQ=255)
                                                                         tttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                  <  1:2974183/148‑1 (MQ=255)
                                                                            gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAAt                                                               <  2:954633/148‑1 (MQ=255)
                                                                               ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTc                                                            <  2:709744/148‑1 (MQ=255)
                                                                                gCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTcc                                                           <  1:759921/148‑1 (MQ=255)
                                                                                gCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTcc                                                           <  1:2843347/148‑1 (MQ=255)
                                                                                  gAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGt                                                         >  2:2623341/1‑148 (MQ=255)
                                                                                   aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                        >  2:1181752/1‑148 (MQ=255)
                                                                                    aaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaa                                                       >  2:594952/1‑148 (MQ=255)
                                                                                     aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                      >  2:1052620/1‑148 (MQ=255)
                                                                                     aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                      <  2:819086/148‑1 (MQ=255)
                                                                                        tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                   >  1:9800/1‑148 (MQ=255)
                                                                                        tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                   <  1:262651/148‑1 (MQ=255)
                                                                                        tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                   >  2:184849/1‑148 (MQ=255)
                                                                                           gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCg                                                >  2:173827/1‑148 (MQ=255)
                                                                                           gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCg                                                >  2:1786897/1‑148 (MQ=255)
                                                                                            aagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGa                                               >  2:2093233/1‑148 (MQ=255)
                                                                                              gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                             <  1:1374815/148‑1 (MQ=255)
                                                                                              gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                             >  2:2870449/1‑148 (MQ=255)
                                                                                               ttACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATa                                            >  2:513710/1‑148 (MQ=255)
                                                                                                tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAg                                           >  2:1089910/1‑148 (MQ=255)
                                                                                                 aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAgg                                          >  1:2682763/1‑148 (MQ=255)
                                                                                                 aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAgg                                          <  1:2750710/148‑1 (MQ=255)
                                                                                                  cATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAggg                                         <  2:860459/148‑1 (MQ=255)
                                                                                                    tCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGtt                                       <  1:969392/148‑1 (MQ=255)
                                                                                                     cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGACTAGGGTTa                                      >  2:3322643/1‑148 (MQ=255)
                                                                                                      aCCTTACGTCTACACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTaa                                     <  2:1334689/148‑1 (MQ=255)
                                                                                                       ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                    <  1:2789409/148‑1 (MQ=255)
                                                                                                       ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                    >  1:368832/1‑148 (MQ=255)
                                                                                                       ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                    <  1:1253860/148‑1 (MQ=255)
                                                                                                       ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                    >  2:1805493/1‑148 (MQ=255)
                                                                                                        cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc                                   <  1:1054463/148‑1 (MQ=255)
                                                                                                        cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc                                   <  2:1637193/148‑1 (MQ=255)
                                                                                                        cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc                                   <  1:808249/148‑1 (MQ=255)
                                                                                                        cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc                                   >  1:1433397/1‑148 (MQ=255)
                                                                                                              tCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACACGCATCCTTCAAATAAATCTGCTTGGGAATGTCCGTACAGTGGCGAATAGGGTTCATCCACGTc                             >  1:688012/1‑148 (MQ=255)
                                                                                                               cTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGACTAGGGTTAATCCACGTCa                            >  2:2747855/1‑148 (MQ=255)
                                                                                                                   aCATCGCGTACGCCGCTGACCCTGTAGTGGCCGCTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                        <  2:3287051/148‑1 (MQ=255)
                                                                                                                    cATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                       <  2:502098/148‑1 (MQ=255)
                                                                                                                    cATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                       >  2:56027/1‑148 (MQ=255)
                                                                                                                      tCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTg                     >  2:846186/1‑148 (MQ=255)
                                                                                                                            aCGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAACTCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCa               <  1:1177329/148‑1 (MQ=255)
                                                                                                                              gccgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATa             <  1:589446/148‑1 (MQ=255)
                                                                                                                              gccgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATa             >  2:615617/1‑148 (MQ=255)
                                                                                                                                    gACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCa       <  2:3056200/148‑1 (MQ=255)
                                                                                                                                      cccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATg     <  2:964950/148‑1 (MQ=255)
                                                                                                                                       ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt    >  1:2939039/1‑148 (MQ=255)
                                                                                                                                       ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt    >  1:2407140/1‑148 (MQ=255)
                                                                                                                                       ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt    >  2:1845804/1‑148 (MQ=255)
                                                                                                                                       ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGGAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt    >  1:2938080/1‑148 (MQ=255)
                                                                                                                                        cTGTAGTGGCCGGTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGtt   <  1:1464017/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTT  >  CP009273/820363‑820647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑