BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	837,716	G→T	100%	intergenic (‑204/‑72)	mcbA ← / → rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	837,716	0	G	T	96.7%	757.1 / 14.0	244	intergenic (‑204/‑72)	mcbA/rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base G (4/4); new base T (144/92); total (148/96)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

AAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGT  >  CP009273/837574‑837859

 aaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAAc                                                                                                                                           >  1:1191678/1‑148 (MQ=255)

   tGACGCAAAAAATTGACAGAGATTTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGc                                                                                                                                         <  2:2603047/148‑1 (MQ=255)

   tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGc                                                                                                                                         >  2:1437941/1‑148 (MQ=255)

   tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGc                                                                                                                                         <  2:2359545/148‑1 (MQ=255)

    gACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCa                                                                                                                                        <  2:1794867/148‑1 (MQ=255)

          aaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGa                                                                                                                                  <  2:741766/148‑1 (MQ=255)

            aaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGata                                                                                                                                <  1:1046299/148‑1 (MQ=255)

            aaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGata                                                                                                                                >  2:42757/1‑148 (MQ=255)

                  aCAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAcc                                                                                                                          <  1:1718795/148‑1 (MQ=255)

                  aCAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAcc                                                                                                                          <  2:3273105/148‑1 (MQ=255)

                      agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGt                                                                                                                      >  2:45815/1‑148 (MQ=255)

                      agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGt                                                                                                                      >  2:2208240/1‑148 (MQ=255)

                          gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcgcg                                                                                                                  >  2:377369/1‑148 (MQ=255)

                          gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcgcg                                                                                                                  >  2:3440959/1‑148 (MQ=255)

                           tgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGt                                                                                                                 <  1:2708088/148‑1 (MQ=255)

                               tATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATg                                                                                                             <  1:200458/148‑1 (MQ=255)

                                 ttttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATgtg                                                                                                           >  1:597184/1‑146 (MQ=255)

                                 ttttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCg                                                                                                           >  2:836045/1‑148 (MQ=255)

                                    tttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGt                                                                                                        <  2:2913129/148‑1 (MQ=255)

                                     ttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTa                                                                                                       >  2:3346335/1‑148 (MQ=255)

                                         aCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                   <  1:364463/148‑1 (MQ=255)

                                         aCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                   >  2:977034/1‑148 (MQ=255)

                                         aCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                   >  2:306205/1‑148 (MQ=255)

                                            atatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcg                                                                                                >  2:1110118/1‑148 (MQ=255)

                                            atatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcg                                                                                                >  1:2883296/1‑148 (MQ=255)

                                                atTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATCGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccgc                                                                                            <  1:1484045/148‑1 (MQ=255)

                                                atTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccgc                                                                                            >  2:624479/1‑148 (MQ=255)

                                                 tttttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgct                                                                                           >  1:319962/1‑148 (MQ=255)

                                                   tttAAAATATAATACAAATGAATGAAATTCATAATGCTGATTTTTATGTTGTATTGCCATAAACCATAGACATTTTTAGTTTTTTTGGAATTATAAACGCAGCCATTTATTTACGCTGTCGCGTTATGCTGGTATATTACGCCgcttc                                                                                         <  2:1520112/148‑3 (MQ=255)

                                                     tACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGACTTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCtt                                                                                       >  2:2034875/1‑148 (MQ=255)

                                                     tACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGGTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCtt                                                                                       >  2:2929145/1‑148 (MQ=255)

                                                      aCAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTa                                                                                      >  2:173186/1‑148 (MQ=255)

                                                         aaaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGACATAAACCATAGCCATGTTAAGGTTTAGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCTCGTTATGCGGGTATCTTACGCCGCTGCTTAAAg                                                                                   >  2:1718855/1‑148 (MQ=255)

                                                          aaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAgg                                                                                  >  1:744384/1‑148 (MQ=255)

                                                           aTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGga                                                                                 >  2:337962/1‑148 (MQ=255)

                                                            tAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                <  1:107660/148‑1 (MQ=255)

                                                            tAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                <  1:2506496/148‑1 (MQ=255)

                                                             aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               >  1:1130707/1‑148 (MQ=255)

                                                             aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               <  1:306205/148‑1 (MQ=255)

                                                             aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               >  1:3114471/1‑148 (MQ=255)

                                                             aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               <  2:1994305/148‑1 (MQ=255)

                                                              aGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGaa                                                                              >  1:3448475/1‑148 (MQ=255)

                                                               gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAAt                                                                             >  2:2651327/1‑148 (MQ=255)

                                                               gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAAt                                                                             >  1:224237/1‑148 (MQ=255)

                                                                aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            <  2:1482801/148‑1 (MQ=255)

                                                                aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            >  1:961725/1‑148 (MQ=255)

                                                                aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            >  1:960717/1‑148 (MQ=255)

                                                                 cAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGc                                                                           >  1:2766688/1‑148 (MQ=255)

                                                                   aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa                                                                         <  2:1410413/148‑1 (MQ=255)

                                                                   aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa                                                                         <  1:2208240/148‑1 (MQ=255)

                                                                    aTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTAt                                                                        >  1:51727/1‑148 (MQ=255)

                                                                        aTGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                    >  1:2757357/1‑148 (MQ=255)

                                                                          gACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGc                                                                  <  2:1195882/148‑1 (MQ=255)

                                                                          gACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGc                                                                  >  1:620522/1‑148 (MQ=255)

                                                                          gACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGc                                                                  <  2:674992/148‑1 (MQ=255)

                                                                          gACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGc                                                                  >  1:1633072/1‑148 (MQ=255)

                                                                           aCATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGcc                                                                 >  2:1852969/1‑148 (MQ=255)

                                                                               gCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGa                                                             >  1:652606/1‑148 (MQ=255)

                                                                                cATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaa                                                            <  1:2899961/148‑1 (MQ=255)

                                                                                 aTAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaaa                                                           >  2:765200/1‑148 (MQ=255)

                                                                                  tAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAAc                                                          >  2:3034404/1‑148 (MQ=255)

                                                                                    aTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCg                                                        <  2:1177588/148‑1 (MQ=255)

                                                                                     tGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCgg                                                       <  2:2294051/148‑1 (MQ=255)

                                                                                      gCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCggg                                                      >  2:2168464/1‑148 (MQ=255)

                                                                                       cTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCgggg                                                     <  1:1214059/148‑1 (MQ=255)

                                                                                           tttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGc                                                 <  2:1275509/148‑1 (MQ=255)

                                                                                            ttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                <  2:1452405/148‑1 (MQ=255)

                                                                                            ttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                >  2:1301331/1‑148 (MQ=255)

                                                                                                  gatgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCcgcg                                          <  1:207470/148‑1 (MQ=255)

                                                                                                   atgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCgcgc                                         >  2:3530153/1‑148 (MQ=255)

                                                                                                      aaTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGACTTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAAc                                      >  2:2613117/1‑148 (MQ=255)

                                                                                                       aTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAAcc                                     >  2:96293/1‑148 (MQ=255)

                                                                                                       aTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAAcc                                     >  1:43390/1‑148 (MQ=255)

                                                                                                        ttGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCg                                    >  2:1617669/1‑148 (MQ=255)

                                                                                                         tGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATNCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGt                                   >  1:1808285/1‑148 (MQ=255)

                                                                                                         tGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGt                                   >  1:746476/1‑148 (MQ=255)

                                                                                                         tGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGt                                   >  2:1753548/1‑148 (MQ=255)

                                                                                                          gCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtc                                  <  1:829318/148‑1 (MQ=255)

                                                                                                          gCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtc                                  <  1:2616671/148‑1 (MQ=255)

                                                                                                           ccATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtca                                 >  1:2577506/1‑148 (MQ=255)

                                                                                                            cATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcat                                >  1:633574/1‑148 (MQ=255)

                                                                                                             aTAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcatc                               >  1:1295858/1‑148 (MQ=255)

                                                                                                               aaaCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                             <  1:322843/148‑1 (MQ=255)

                                                                                                               aaaCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                             >  1:1391548/1‑148 (MQ=255)

                                                                                                                 aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAg                           >  1:3160915/1‑148 (MQ=255)

                                                                                                                  ccATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGc                          >  1:3440801/1‑148 (MQ=255)

                                                                                                                  ccATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGc                          >  2:656850/1‑148 (MQ=255)

                                                                                                                          aTGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGa                  <  1:2952534/148‑1 (MQ=255)

                                                                                                                          aTGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGa                  <  1:338103/148‑1 (MQ=255)

                                                                                                                            gTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtc                <  2:1579141/148‑1 (MQ=255)

                                                                                                                             ttAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtct               >  1:204815/1‑148 (MQ=255)

                                                                                                                               aaGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCg             >  1:188851/1‑148 (MQ=255)

                                                                                                                                aGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGc            <  2:910671/148‑1 (MQ=255)

                                                                                                                                     ttGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGc       <  1:2425595/148‑1 (MQ=255)

                                                                                                                                      tGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCt      <  2:3142629/148‑1 (MQ=255)

                                                                                                                                       ggggAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCtt     <  1:1358921/148‑1 (MQ=255)

                                                                                                                                       ggggAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCtt     <  1:2410172/148‑1 (MQ=255)

                                                                                                                                        gggAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCttt    <  2:224237/148‑1 (MQ=255)

                                                                                                                                         ggAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTg   >  2:2515525/1‑148 (MQ=255)

                                                                                                                                          gAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCGTTGt  >  1:748451/1‑148 (MQ=255)

AAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGT  >  CP009273/837574‑837859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`