Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 943,573 G→T 100% A297S (GCC→TCC)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273943,5730GT100.0% 798.4 / NA 240A297S (GCC→TCC) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (125/115);  total (125/115)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCT  >  CP009273/943428‑943717
                                                                                                                                                 |                                                                                                                                                
ttGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTcc                                                                                                                                                <  1:1379011/148‑1 (MQ=255)
 tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCa                                                                                                                                               <  1:1755041/148‑1 (MQ=255)
  gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAt                                                                                                                                              <  2:3416070/148‑1 (MQ=255)
    gCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTg                                                                                                                                            <  2:1202704/148‑1 (MQ=255)
     ccATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGc                                                                                                                                           >  1:1243510/1‑148 (MQ=255)
     ccATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGc                                                                                                                                           >  1:26694/1‑148 (MQ=255)
      cATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCg                                                                                                                                          <  2:594510/148‑1 (MQ=255)
         tCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAt                                                                                                                                       >  1:553033/1‑148 (MQ=255)
                 cGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTggg                                                                                                                               >  2:2084313/1‑148 (MQ=255)
                    tGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTAGTCCATTGCGAATACCCTGGGACa                                                                                                                            <  2:1707025/148‑1 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                           >  1:1761770/1‑148 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                           <  1:2082533/148‑1 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                           <  2:3565311/148‑1 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                           <  1:2843452/148‑1 (MQ=255)
                      gCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAg                                                                                                                          <  1:3402343/148‑1 (MQ=255)
                         aTGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAg                                                                                                                       >  1:2849254/1‑148 (MQ=255)
                         aTGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAg                                                                                                                       <  1:2531300/148‑1 (MQ=255)
                           ggggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcg                                                                                                                     <  1:3516202/148‑1 (MQ=255)
                            gggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcgg                                                                                                                    >  1:1904763/1‑148 (MQ=255)
                             ggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggc                                                                                                                   >  1:3034482/1‑148 (MQ=255)
                              gatgatGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggcg                                                                                                                  >  2:1286737/1‑148 (MQ=255)
                                   tgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGt                                                                                                             <  1:1692422/148‑1 (MQ=255)
                                     tttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAt                                                                                                           >  1:3862/1‑148 (MQ=255)
                                      ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                          >  2:1062643/1‑148 (MQ=255)
                                      ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                          >  2:1062626/1‑148 (MQ=255)
                                      ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                          <  2:3083679/148‑1 (MQ=255)
                                      ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                          <  1:1570543/148‑1 (MQ=255)
                                        gATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtct                                                                                                        <  1:2901569/148‑1 (MQ=255)
                                         aTTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                       >  1:3024282/1‑148 (MQ=255)
                                          ttCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCg                                                                                                      >  1:119247/1‑148 (MQ=255)
                                           tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                     >  1:2956308/1‑148 (MQ=255)
                                           tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                     <  2:3481734/148‑1 (MQ=255)
                                           tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAACCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                     >  2:3063678/1‑148 (MQ=255)
                                              cgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTg                                                                                                  >  2:324980/1‑148 (MQ=255)
                                                 aaTATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                               <  2:3009427/148‑1 (MQ=255)
                                                 aaTATCCCGGATGACTTAATGACAAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                               >  2:1290022/1‑148 (MQ=255)
                                                    atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGTTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGccc                                                                                            <  1:924672/148‑1 (MQ=255)
                                                     tCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGcccc                                                                                           >  1:2438361/1‑148 (MQ=255)
                                                        cGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCt                                                                                        <  1:237658/148‑1 (MQ=255)
                                                           aTGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaa                                                                                     >  1:3474586/1‑148 (MQ=255)
                                                           aTGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaa                                                                                     >  2:2232438/1‑148 (MQ=255)
                                                            tGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGccc                                                                                            <  2:2938288/140‑1 (MQ=255)
                                                            tGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaaa                                                                                    >  1:1445338/1‑148 (MQ=255)
                                                             gACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAg                                                                                   >  1:311205/1‑148 (MQ=255)
                                                                   aTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAt                                                                             <  2:3034482/148‑1 (MQ=255)
                                                                   aTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAt                                                                             <  2:544735/148‑1 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  1:495314/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  2:404213/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  2:2469581/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            <  1:867329/148‑1 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            <  1:1291935/148‑1 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAAAATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            <  1:2655060/148‑1 (MQ=255)
                                                                     gACCAACGGTCATTATTACGCCTTACAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTa                                                                           <  1:3397507/148‑1 (MQ=255)
                                                                     gACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTa                                                                           >  1:3236084/1‑148 (MQ=255)
                                                                      aCCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAg                                                                          >  1:706756/1‑148 (MQ=255)
                                                                       ccAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAgg                                                                         >  1:1143645/1‑148 (MQ=255)
                                                                       ccAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAgg                                                                         <  1:2944593/148‑1 (MQ=255)
                                                                        cAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGt                                                                        <  2:1234671/148‑1 (MQ=255)
                                                                         aaCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCGTGTTTTGGGGATTGATGCCCCCCTTAAAGTGCTATTAGGTg                                                                       >  1:651556/1‑148 (MQ=255)
                                                                          aCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGa                                                                      <  2:982488/148‑1 (MQ=255)
                                                                             gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                   <  2:3539760/148‑1 (MQ=255)
                                                                              tCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                  >  1:2241575/1‑148 (MQ=255)
                                                                                     tACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCa                                                           >  1:2802861/1‑148 (MQ=255)
                                                                                       cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaaa                                                         <  1:787707/148‑1 (MQ=255)
                                                                                        gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAat                                                        >  2:2649715/1‑148 (MQ=255)
                                                                                           tttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatata                                                     <  2:552753/148‑1 (MQ=255)
                                                                                            ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatatat                                                    >  2:853899/1‑148 (MQ=255)
                                                                                              cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTc                                                  <  2:938111/148‑1 (MQ=255)
                                                                                                gAAGCTGGGCTAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                >  1:104949/1‑148 (MQ=255)
                                                                                                gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                >  1:1706817/1‑148 (MQ=255)
                                                                                                gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                >  2:2466601/1‑148 (MQ=255)
                                                                                                  aGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagc                                              >  2:669270/1‑148 (MQ=255)
                                                                                                    cTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcca                                            >  2:2029231/1‑148 (MQ=255)
                                                                                                          gAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTAt                                      >  1:2322590/1‑148 (MQ=255)
                                                                                                           aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATg                                     >  1:436121/1‑148 (MQ=255)
                                                                                                           aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATg                                     >  2:813370/1‑148 (MQ=255)
                                                                                                           aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATg                                     >  1:1022375/1‑148 (MQ=255)
                                                                                                           aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATg                                     <  2:1439282/148‑1 (MQ=255)
                                                                                                               ttACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGt                                 >  2:1828140/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  1:3205544/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                <  1:2592461/148‑1 (MQ=255)
                                                                                                                   aacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTCTTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                             >  2:2398870/1‑148 (MQ=255)
                                                                                                                   aacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                             >  2:1916235/1‑148 (MQ=255)
                                                                                                                         ggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCtt                       <  2:1975818/148‑1 (MQ=255)
                                                                                                                          gTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                      <  2:295413/148‑1 (MQ=255)
                                                                                                                           tAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                     >  2:2773768/1‑148 (MQ=255)
                                                                                                                           tAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                     <  2:2709513/148‑1 (MQ=255)
                                                                                                                              tACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGt                  >  1:970901/1‑148 (MQ=255)
                                                                                                                               aCTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTa                 >  2:1121862/1‑148 (MQ=255)
                                                                                                                               aCTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTa                 >  1:3437092/1‑148 (MQ=255)
                                                                                                                                 tttAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACg               <  2:470509/148‑1 (MQ=255)
                                                                                                                                  ttAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGTTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGc              >  2:2297498/1‑148 (MQ=255)
                                                                                                                                      tGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGt          <  2:1858507/148‑1 (MQ=255)
                                                                                                                                        gTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTTCGCCCGTTa        >  2:2916936/1‑148 (MQ=255)
                                                                                                                                        gTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa        <  2:2836737/148‑1 (MQ=255)
                                                                                                                                        gTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa        <  2:1746908/148‑1 (MQ=255)
                                                                                                                                           aTTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATg     >  2:2369136/1‑148 (MQ=255)
                                                                                                                                            tttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATgg    >  1:1622443/1‑148 (MQ=255)
                                                                                                                                             ttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGc   >  2:687082/1‑148 (MQ=255)
                                                                                                                                             ttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGc   >  2:780207/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCT  >  CP009273/943428‑943717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑