Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 992,767 C→A 94.9% D68Y (GAT→TAT)  ssuE ← NAD(P)H‑dependent FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273992,7670CA94.9% 608.9 / 21.3 214D68Y (GAT→TAT) ssuENAD(P)H‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base A (101/102);  total (107/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 214 total aligned reads displayed.

CCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCC  >  CP009273/992623‑992909
                                                                                                                                                |                                                                                                                                              
ccAGCAGATGGGCGACGGTTCCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGc                                                                                                                                             <  2:227509/148‑1 (MQ=255)
   gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTg                                                                                                                                          <  1:425875/148‑1 (MQ=255)
   gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTg                                                                                                                                          <  2:2410198/148‑1 (MQ=255)
    cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGt                                                                                                                                         <  2:650974/148‑1 (MQ=255)
    cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGt                                                                                                                                         >  1:3445175/1‑148 (MQ=255)
       aTGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGc                                                                                                                                      >  2:1213602/1‑148 (MQ=255)
         gggCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAg                                                                                                                                    <  2:3100615/148‑1 (MQ=255)
         gggCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAg                                                                                                                                    <  1:143808/148‑1 (MQ=255)
            ccACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTg                                                                                                                                 <  2:285389/148‑1 (MQ=255)
            ccACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTg                                                                                                                                 <  1:2320283/148‑1 (MQ=255)
             cACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGt                                                                                                                                >  1:3116908/1‑148 (MQ=255)
              aCGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGtt                                                                                                                               <  2:1124251/148‑1 (MQ=255)
                ggTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCg                                                                                                                             <  1:2009263/148‑1 (MQ=255)
                ggTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCg                                                                                                                             <  1:2009194/148‑1 (MQ=255)
                  tACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGt                                                                                                                           >  1:3007674/1‑148 (MQ=255)
                    ccGCCCGTCGCCAGCGGTAGCACCACTTAGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAAAGCACCGGAAAAGGCGGCTTTATACACAGGCGTGGCGACACTCAGCCCAAAGGCCTGTTGCAGCTGTTCGGTGa                                                                                                                         >  2:1654182/1‑148 (MQ=255)
                     cGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGaa                                                                                                                        >  2:2436146/1‑148 (MQ=255)
                     cGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGaa                                                                                                                        >  1:2803132/1‑148 (MQ=255)
                      gCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAg                                                                                                                       <  2:3550978/148‑1 (MQ=255)
                        ccGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGt                                                                                                                     <  2:1805858/148‑1 (MQ=255)
                           tCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCtt                                                                                                                  <  2:479025/148‑1 (MQ=255)
                            cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACCCAGGCGTGGCGCCAATCCGCCCATCGGCCTGTGGCAGCTGTGCGGTGAAGGTCTTg                                                                                                                 >  2:1685822/1‑148 (MQ=255)
                                aGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTg                                                                                                             <  2:1453670/148‑1 (MQ=255)
                                    gTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCgg                                                                                                         >  2:257490/1‑148 (MQ=255)
                                     tAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGa                                                                                                        <  1:894038/148‑1 (MQ=255)
                                      aGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGAc                                                                                                       <  2:1640657/148‑1 (MQ=255)
                                      aGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGAc                                                                                                       <  1:1795808/148‑1 (MQ=255)
                                       gCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACt                                                                                                      >  2:1517027/1‑148 (MQ=255)
                                        caccacTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTa                                                                                                     >  1:53832/1‑148 (MQ=255)
                                           cacTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCg                                                                                                  <  1:2353521/148‑1 (MQ=255)
                                             cTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGaa                                                                                                >  2:74232/1‑148 (MQ=255)
                                             cTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGaa                                                                                                >  2:1881016/1‑148 (MQ=255)
                                               ttGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAAc                                                                                              >  1:1677301/1‑148 (MQ=255)
                                                  ccTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTTTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAg                                                                                           <  1:2536717/148‑1 (MQ=255)
                                                       cAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaa                                                                                      <  2:2050448/148‑1 (MQ=255)
                                                       cAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaa                                                                                      <  2:2050327/148‑1 (MQ=255)
                                                        aaaGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaaa                                                                                     >  2:2597178/1‑148 (MQ=255)
                                                         aaGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAg                                                                                    <  2:3256281/148‑1 (MQ=255)
                                                         aaGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAg                                                                                    <  2:2671775/148‑1 (MQ=255)
                                                          aGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGt                                                                                   <  2:564482/148‑1 (MQ=255)
                                                          aGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGt                                                                                   >  1:3503314/1‑148 (MQ=255)
                                                              cgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAt                                                                               <  1:1745389/148‑1 (MQ=255)
                                                                 tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                            <  2:1271296/148‑1 (MQ=255)
                                                                 tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                            <  1:2367990/148‑1 (MQ=255)
                                                                 tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                            >  1:1467744/1‑148 (MQ=255)
                                                                 tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                            <  1:1876963/148‑1 (MQ=255)
                                                                    ggCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCg                                                                         >  2:1657747/1‑148 (MQ=255)
                                                                    ggCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCg                                                                         >  1:1695157/1‑148 (MQ=255)
                                                                     gcagcaGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCgg                                                                        <  2:2310224/148‑1 (MQ=255)
                                                                     gcagcaGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCgg                                                                        <  2:1187638/148‑1 (MQ=255)
                                                                     gcagcaGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCgg                                                                        <  1:1331268/148‑1 (MQ=255)
                                                                     gcagcaGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCgg                                                                        >  1:2983522/1‑148 (MQ=255)
                                                                        gcagGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGc                                                                     >  2:1738438/1‑148 (MQ=255)
                                                                          agGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCCGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGa                                                                   <  2:3282294/148‑1 (MQ=255)
                                                                           ggTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGaa                                                                  >  2:1969090/1‑148 (MQ=255)
                                                                           ggTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGaa                                                                  <  1:95529/148‑1 (MQ=255)
                                                                            gTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAg                                                                 <  1:380371/148‑1 (MQ=255)
                                                                             tCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGt                                                                <  2:2234947/148‑1 (MQ=255)
                                                                              cGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGtt                                                               >  2:2312578/1‑148 (MQ=255)
                                                                                  cAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGc                                                           <  2:1432537/148‑1 (MQ=255)
                                                                                      gTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAt                                                       <  1:3311767/148‑1 (MQ=255)
                                                                                      gTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAt                                                       <  1:3259082/148‑1 (MQ=255)
                                                                                       ttttCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAtt                                                      >  2:2501385/1‑148 (MQ=255)
                                                                                         ttCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTcc                                                    <  2:3186890/148‑1 (MQ=255)
                                                                                         ttCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTcc                                                    <  2:1458704/148‑1 (MQ=255)
                                                                                           cAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAg                                                  <  1:599280/148‑1 (MQ=255)
                                                                                            aaCGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCGGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGt                                                 <  1:2628442/148‑1 (MQ=255)
                                                                                                cACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACTAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATa                                             >  1:194050/1‑148 (MQ=255)
                                                                                                 aCCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaa                                            <  1:2919456/148‑1 (MQ=255)
                                                                                                  ccGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaaa                                           >  1:1640549/1‑148 (MQ=255)
                                                                                                   cGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc                                          <  1:157642/148‑1 (MQ=255)
                                                                                                       aTAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACctct                                      >  1:2743803/1‑148 (MQ=255)
                                                                                                        tAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTa                                     >  2:2903204/1‑148 (MQ=255)
                                                                                                          ggcggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACa                                   >  2:3114868/1‑148 (MQ=255)
                                                                                                           gcggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACAt                                  >  1:3006525/1‑148 (MQ=255)
                                                                                                           gcggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACAt                                  >  2:423798/1‑148 (MQ=255)
                                                                                                             ggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATcc                                <  1:74232/148‑1 (MQ=255)
                                                                                                             ggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATcc                                <  2:139394/148‑1 (MQ=255)
                                                                                                             ggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATcc                                <  1:47176/148‑1 (MQ=255)
                                                                                                               cTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAg                              >  2:2864894/1‑148 (MQ=255)
                                                                                                                   ataCACAGGCGTGGCGACAATCAGCCCATAGTCCTGTTGCAGCTGTTCGGTGAAGGTGTTGAGTGCCGGTGTATCGAATCGAGCATTTAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCTGTGATAAACCTCTACATCCAGGCCa                          <  1:1909962/148‑1 (MQ=255)
                                                                                                                     acacAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAtt                        <  1:1151786/148‑1 (MQ=255)
                                                                                                                       acaGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTa                      <  2:3007674/148‑1 (MQ=255)
                                                                                                                        caGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAg                     <  1:596496/148‑1 (MQ=255)
                                                                                                                         aGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGt                    >  1:2039074/1‑148 (MQ=255)
                                                                                                                          ggCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGtt                   >  2:2812633/1‑148 (MQ=255)
                                                                                                                           gCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGACGTTTTGCAGATTCCAGTGATCAACCTCTACATCGAGGCCCGGTAGttt                  >  2:3386202/1‑148 (MQ=255)
                                                                                                                           gCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGttt                  >  2:3386245/1‑148 (MQ=255)
                                                                                                                           gCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGttt                  <  1:2112154/148‑1 (MQ=255)
                                                                                                                                 cGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCcg            >  2:409176/1‑148 (MQ=255)
                                                                                                                                 cGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCcg            <  1:1213602/148‑1 (MQ=255)
                                                                                                                                    cAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCgcgc         >  1:1386124/1‑148 (MQ=255)
                                                                                                                                    cAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCgcgc         >  1:1385300/1‑148 (MQ=255)
                                                                                                                                      aTCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCat       >  1:1546327/1‑148 (MQ=255)
                                                                                                                                       tCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCata      <  2:2711916/148‑1 (MQ=255)
                                                                                                                                       tCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGCGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCata      <  1:2874718/148‑1 (MQ=255)
                                                                                                                                        cAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCatat     >  2:2019204/1‑148 (MQ=255)
                                                                                                                                          gCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTc   >  1:845032/1‑148 (MQ=255)
                                                                                                                                          gCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTc   >  1:915974/1‑148 (MQ=255)
                                                                                                                                           cccATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTcc  >  1:2607815/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCC  >  CP009273/992623‑992909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑