BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,023,028	G→T	5.3%	H68N (CAT→AAT)	yccT ←	UPF0319 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,023,028	0	G	T	5.3%	742.0 / 11.9	199	H68N (CAT→AAT)	yccT	UPF0319 family protein
Reads supporting (aligned to +/- strand): ref base G (87/101); new base T (6/5); total (93/106)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CAAGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCAAA  >  CP009273/1022881‑1023175

  aGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATTaa                                                                                                                                                   <  1:3273061/148‑1 (MQ=255)

  aGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGaa                                                                                                                                                   <  2:511743/148‑1 (MQ=255)

  aGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGaa                                                                                                                                                   >  1:2580473/1‑148 (MQ=255)

  aGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGaa                                                                                                                                                   <  2:1379306/148‑1 (MQ=255)

  aGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGaa                                                                                                                                                   <  2:1379370/148‑1 (MQ=255)

   ggcgcggcgcGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATTAAt                                                                                                                                                  <  2:1235923/148‑1 (MQ=255)

   ggcgcggcgcGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAAt                                                                                                                                                  >  1:3547429/1‑148 (MQ=255)

   ggcgcggcgcGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAAt                                                                                                                                                  >  1:889418/1‑148 (MQ=255)

   ggcgcggcgcGACATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAAt                                                                                                                                                  >  2:3174117/1‑148 (MQ=255)

       cggcgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtc                                                                                                                                              <  2:608654/148‑1 (MQ=255)

        ggcgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATTAATCGtct                                                                                                                                             <  2:1643847/148‑1 (MQ=255)

         gcgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACGATTGGAAAGATGAATCGtctt                                                                                                                                            >  1:2207045/1‑148 (MQ=255)

          cgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtcttc                                                                                                                                           <  2:3293311/148‑1 (MQ=255)

          cgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtcttc                                                                                                                                           <  2:1515390/148‑1 (MQ=255)

          cgcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtcttc                                                                                                                                           >  1:2661144/1‑148 (MQ=255)

           gcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtcttct                                                                                                                                          <  1:448579/148‑1 (MQ=255)

           gcggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGtcttct                                                                                                                                          <  1:2168459/148‑1 (MQ=255)

             ggcATCATAATGGTTAGCTTCCCGCTCATTCTACAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACAAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGATTCGTCTTCTCa                                                                                                                                        <  2:3331501/148‑1 (MQ=255)

             ggcATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCa                                                                                                                                        <  1:78144/148‑1 (MQ=255)

               cATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAAc                                                                                                                                      >  2:1078829/1‑148 (MQ=255)

                 tCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATNAAAACTGACCACAAGCGGTGGGGAGATATACAGCCGTTCTTCCAGAATGTGAACAAGAATCGTCTTATAAAcgc                                                                                                                                    >  1:1806925/1‑148 (MQ=255)

                    aaaTGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGaa                                                                                                                                 >  2:2640039/1‑148 (MQ=255)

                     aaTGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGaaa                                                                                                                                >  1:1296434/1‑148 (MQ=255)

                          ttAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTa                                                                                                                           >  2:1255041/1‑148 (MQ=255)

                           tAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTaa                                                                                                                          >  1:2990933/1‑148 (MQ=255)

                           tAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTaa                                                                                                                          <  1:3396916/148‑1 (MQ=255)

                           tAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTaa                                                                                                                          >  1:445198/1‑148 (MQ=255)

                               ttCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGa                                                                                                                      >  2:2811043/1‑148 (MQ=255)

                                tCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGAt                                                                                                                     >  2:1500247/1‑148 (MQ=255)

                                tCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGGGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAAAGCGAAACACTAACAGAt                                                                                                                     >  2:2986041/1‑148 (MQ=255)

                                     cTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGt                                                                                                                >  2:1123140/1‑148 (MQ=255)

                                     cTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGt                                                                                                                <  1:702204/148‑1 (MQ=255)

                                      tCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATTAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTc                                                                                                               >  2:3273371/1‑148 (MQ=255)

                                      tCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTc                                                                                                               >  2:1985520/1‑148 (MQ=255)

                                       cATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTcc                                                                                                              <  1:65022/148‑1 (MQ=255)

                                        aTTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCa                                                                                                             <  2:1788994/148‑1 (MQ=255)

                                        aTTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTATTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCa                                                                                                             <  1:282645/148‑1 (MQ=255)

                                          tctcCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCAtt                                                                                                           >  2:243544/1‑148 (MQ=255)

                                            tcCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTAt                                                                                                         >  2:2585038/1‑148 (MQ=255)

                                             ccAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATc                                                                                                        >  2:2710974/1‑148 (MQ=255)

                                             ccAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATc                                                                                                        <  2:889418/148‑1 (MQ=255)

                                             ccAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATc                                                                                                        <  1:1988997/148‑1 (MQ=255)

                                             ccAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATc                                                                                                        >  1:2912867/1‑148 (MQ=255)

                                                ggCGAGGCAGGCGAAAATTGTCCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAg                                                                                                     <  2:2945009/148‑1 (MQ=255)

                                                 gCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACGGATGCGGTCCATTATCCAGt                                                                                                    <  1:85148/148‑1 (MQ=255)

                                                    aggcaggcGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGTATCGTCTTCTCAACGCCAAACACTAACAGATGCGGGCCATTATCCACTTCa                                                                                                 >  1:373467/1‑148 (MQ=255)

                                                    aggcaggcGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCa                                                                                                 <  2:2410759/148‑1 (MQ=255)

                                                      gcaggcGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAAt                                                                                               >  2:3116274/1‑148 (MQ=255)

                                                         ggcGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCt                                                                                            >  1:3523014/1‑148 (MQ=255)

                                                         ggcGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCt                                                                                            >  1:224661/1‑148 (MQ=255)

                                                               aaTTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGc                                                                                      >  1:410171/1‑148 (MQ=255)

                                                                aTTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCa                                                                                     <  1:2271543/148‑1 (MQ=255)

                                                                     ccTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACcgcg                                                                                <  2:2788734/148‑1 (MQ=255)

                                                                     ccTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACcgcg                                                                                <  2:2171506/148‑1 (MQ=255)

                                                                      cTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCgcgc                                                                               >  2:3025030/1‑148 (MQ=255)

                                                                        ggTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCgcag                                                                             <  1:347925/148‑1 (MQ=255)

                                                                         gTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTATCAAAGCGAAACACTAAATGATTCGGTCCATTATACAGTTCAATGCTGTCCGCACCGCgcagc                                                                            >  1:1584797/1‑148 (MQ=255)

                                                                           tgatgaGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGcagcag                                                                          >  1:246111/1‑148 (MQ=255)

                                                                             atgaGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCagag                                                                        >  1:2331582/1‑148 (MQ=255)

                                                                             atgaGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTAAATGCTGTCCGCACCGCGCCGCagag                                                                        >  2:304124/1‑148 (MQ=255)

                                                                              tgaGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCagaga                                                                       >  2:1890626/1‑148 (MQ=255)

                                                                                  cTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTg                                                                   >  1:1469464/1‑148 (MQ=255)

                                                                                  cTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTg                                                                   >  2:1998654/1‑148 (MQ=255)

                                                                                      gTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGaaa                                                               <  2:2356439/148‑1 (MQ=255)

                                                                                       tATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTATCAACGCGAAACACTAACTTATGCGGTCCATTATACAGTTCAATTGTTTCCGCACCGCGCAGCAGAGAGCTGGAAAc                                                              >  1:1006331/1‑148 (MQ=255)

                                                                                         ttAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACct                                                            <  2:3035192/148‑1 (MQ=255)

                                                                                           aaaaCTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACcttc                                                          <  1:3130018/148‑1 (MQ=255)

                                                                                            aaaCTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACcttct                                                         <  2:3369695/148‑1 (MQ=255)

                                                                                            aaaCTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACcttct                                                         >  2:696476/1‑148 (MQ=255)

                                                                                              aCTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTg                                                       <  1:2482354/148‑1 (MQ=255)

                                                                                                  accaccAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGt                                                   <  1:1823068/148‑1 (MQ=255)

                                                                                                    caccaGCGGTGGGGAGATATACAGCCGTTCTTCACGATTGGGAAGATGAATCGTCTTCTCAACGCGAAACACAAACTGATGCGGGCCATTATACAGTTAAAAGCTGTCCGCCCCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCg                                                 >  1:618208/1‑148 (MQ=255)

                                                                                                      ccaGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAg                                               <  1:1878277/148‑1 (MQ=255)

                                                                                                      ccaGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAg                                               <  1:1878108/148‑1 (MQ=255)

                                                                                                       caGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGt                                              >  1:1728652/1‑148 (MQ=255)

                                                                                                          cGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTAcc                                           >  2:375410/1‑148 (MQ=255)

                                                                                                               gggAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCagaag                                      <  1:1255041/148‑1 (MQ=255)

                                                                                                                    atatACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGa                                 <  1:100675/148‑1 (MQ=255)

                                                                                                                    atatACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGa                                 >  2:2749204/1‑148 (MQ=255)

                                                                                                                     tataCAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGAc                                <  1:2611804/148‑1 (MQ=255)

                                                                                                                           gCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGt                          <  1:1109651/148‑1 (MQ=255)

                                                                                                                           gCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGt                          >  1:2032857/1‑148 (MQ=255)

                                                                                                                           gCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGt                          <  1:1109838/148‑1 (MQ=255)

                                                                                                                                 cttcACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAg                    >  2:2604033/1‑148 (MQ=255)

                                                                                                                                     aCTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGc                <  2:3095615/148‑1 (MQ=255)

                                                                                                                                       tATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAg              >  1:151312/1‑148 (MQ=255)

                                                                                                                                        aTTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGc             >  1:1913319/1‑148 (MQ=255)

                                                                                                                                         ttGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCg            >  1:354279/1‑148 (MQ=255)

                                                                                                                                         ttGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCg            >  1:2110717/1‑148 (MQ=255)

                                                                                                                                           ggAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCgtg          <  1:1931563/148‑1 (MQ=255)

                                                                                                                                            gAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCgtgg         >  2:555110/1‑148 (MQ=255)

                                                                                                                                             aaaGATGAATCGTCTTCTCAACGCGAAACACTCACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCgtggt        <  2:344482/148‑1 (MQ=255)

                                                                                                                                             aaaGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCgtggt        <  1:1191410/148‑1 (MQ=255)

                                                                                                                                             aaaGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCgtggt        <  2:344379/148‑1 (MQ=255)

                                                                                                                                                gATTAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGc     >  2:1213739/1‑148 (MQ=255)

                                                                                                                                                gATTAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGc     >  2:1682201/1‑148 (MQ=255)

                                                                                                                                                 aTGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCa    >  2:1865884/1‑148 (MQ=255)

                                                                                                                                                 aTGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCa    >  2:1029449/1‑148 (MQ=255)

                                                                                                                                                  tGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCaa   >  2:288014/1‑148 (MQ=255)

                                                                                                                                                   gAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCaaa  >  1:1039536/1‑148 (MQ=255)

CAAGGCGCGGCGCGGCATCAAAATGGTTAGCTTCCCGCTCATTCTCCAGGCGAGGCAGGCGAAAATTGACCTGGTTGATGAGCTGGGTATTAAAACTGACCACCAGCGGTGGGGAGATATACAGCCGTTCTTCACTATTGGAAAGATGAATCGTCTTCTCAACGCGAAACACTAACTGATGCGGTCCATTATCCAGTTCAATGCTGTCCGCACCGCGCAGCAGAGAGCTGGAAACCTTCTTGCCGTCGAGTACCAGAAGATCGACATCGGTTGAAAGCCGCAGCGTGGTGGCAAA  >  CP009273/1022881‑1023175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`