Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 102,736 C→A 94.9% I315I (ATC→ATA)  ftsZ → GTP‑binding tubulin‑like cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273102,7360CA94.9% 596.2 / 21.6 207I315I (ATC→ATA) ftsZGTP‑binding tubulin‑like cell division protein
Reads supporting (aligned to +/- strand):  ref base C (9/2);  new base A (112/84);  total (121/86)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCG  >  CP009273/102591‑102880
                                                                                                                                                 |                                                                                                                                                
 cTTCGACCTGCGTCTGGAAGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGc                                                                                                                                               <  1:2082892/148‑1 (MQ=255)
  ttCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAAGGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCa                                                                                                                                              <  1:1494313/148‑1 (MQ=255)
    cGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATg                                                                                                                                            <  1:1246908/148‑1 (MQ=255)
    cGACCTGCGTCTGGATGAGTACGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGAATg                                                                                                                                            >  2:879939/1‑148 (MQ=255)
     gACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATgg                                                                                                                                           <  1:2120611/148‑1 (MQ=255)
       ccTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGAc                                                                                                                                         >  2:1342846/1‑148 (MQ=255)
        cTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACa                                                                                                                                        >  2:1170234/1‑148 (MQ=255)
          gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                      >  1:1102705/1‑148 (MQ=255)
          gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                      >  1:3292190/1‑148 (MQ=255)
          gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                      >  1:3292186/1‑148 (MQ=255)
          gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                      >  2:216684/1‑148 (MQ=255)
            gTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTNCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACg                                                                                                                                    <  1:1807055/148‑1 (MQ=255)
                 gATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTg                                                                                                                               >  2:1075819/1‑148 (MQ=255)
                  aTGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGa                                                                                                                              >  2:362150/1‑148 (MQ=255)
                   tGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaa                                                                                                                             <  2:2102843/148‑1 (MQ=255)
                     aGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAAt                                                                                                                           >  2:3113755/1‑148 (MQ=255)
                     aGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAAt                                                                                                                           <  2:2066214/148‑1 (MQ=255)
                      gTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATc                                                                                                                          <  2:1739833/148‑1 (MQ=255)
                       ttCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCa                                                                                                                         >  2:1549887/1‑148 (MQ=255)
                       ttCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCa                                                                                                                         >  2:1550424/1‑148 (MQ=255)
                          gAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCActc                                                                                                                      <  2:1242643/148‑1 (MQ=255)
                           aaaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCActct                                                                                                                     >  1:54283/1‑148 (MQ=255)
                           aaaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCActct                                                                                                                     >  1:55493/1‑148 (MQ=255)
                            aaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTg                                                                                                                    >  2:2961166/1‑148 (MQ=255)
                            aaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTg                                                                                                                    <  2:2895945/148‑1 (MQ=255)
                             aCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTgg                                                                                                                   >  1:2019484/1‑148 (MQ=255)
                             aCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTgg                                                                                                                   <  1:468858/148‑1 (MQ=255)
                                  aggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAcc                                                                                                              <  1:3433525/148‑1 (MQ=255)
                                     taACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAAt                                                                                                           >  2:833978/1‑148 (MQ=255)
                                     taACACAATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAAt                                                                                                           <  2:1307867/148‑1 (MQ=255)
                                        cacCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAg                                                                                                        >  1:1696384/1‑148 (MQ=255)
                                           cATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAg                                                                                                     >  1:2687821/1‑148 (MQ=255)
                                            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAgg                                                                                                    <  2:3240299/148‑1 (MQ=255)
                                            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAgg                                                                                                    <  1:263499/148‑1 (MQ=255)
                                            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAgg                                                                                                    <  2:3209776/148‑1 (MQ=255)
                                             tCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                   >  1:2034183/1‑148 (MQ=255)
                                              ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                  <  1:2109000/148‑1 (MQ=255)
                                                 tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcag                                                                                               <  2:914759/148‑1 (MQ=255)
                                                  gCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagc                                                                                              >  2:1571358/1‑148 (MQ=255)
                                                  gCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagc                                                                                              >  2:3030691/1‑148 (MQ=255)
                                                   cATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagca                                                                                             >  1:504921/1‑148 (MQ=255)
                                                      ttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGcc                                                                                          >  1:988437/1‑148 (MQ=255)
                                                      ttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGcc                                                                                          >  1:680307/1‑148 (MQ=255)
                                                        gCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAg                                                                                        >  2:3164621/1‑148 (MQ=255)
                                                         cTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGt                                                                                       >  1:725569/1‑148 (MQ=255)
                                                            ccGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                    <  2:805455/148‑1 (MQ=255)
                                                              gACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATgg                                                                                  <  2:2632971/148‑1 (MQ=255)
                                                                cAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGAt                                                                                >  1:2790752/1‑148 (MQ=255)
                                                                cAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGAt                                                                                >  1:1731473/1‑148 (MQ=255)
                                                                  aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCg                                                                              <  1:782265/148‑1 (MQ=255)
                                                                  aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCg                                                                              >  1:1018/1‑148 (MQ=255)
                                                                       aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                         >  1:620427/1‑148 (MQ=255)
                                                                       aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                         <  1:1764236/148‑1 (MQ=255)
                                                                        cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACca                                                                        >  1:1629474/1‑148 (MQ=255)
                                                                         tgtgGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcag                                                                       >  2:2326039/1‑148 (MQ=255)
                                                                            ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagcag                                                                    >  1:606598/1‑148 (MQ=255)
                                                                            ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagcag                                                                    >  1:2238061/1‑148 (MQ=255)
                                                                              ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAgcagca                                                                  <  2:2754300/148‑1 (MQ=255)
                                                                                  cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                              <  2:3156317/148‑1 (MQ=255)
                                                                                   ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGa                                                             <  1:711024/148‑1 (MQ=255)
                                                                                     tACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATg                                                           >  1:2618950/1‑148 (MQ=255)
                                                                                      aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATgg                                                          >  1:1837040/1‑148 (MQ=255)
                                                                                       cTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGc                                                         <  2:1238961/148‑1 (MQ=255)
                                                                                         tctcTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTc                                                       >  2:1949954/1‑148 (MQ=255)
                                                                                           tctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCg                                                     <  2:1270140/148‑1 (MQ=255)
                                                                                            ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                    >  1:2332732/1‑148 (MQ=255)
                                                                                            ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                    >  1:2852481/1‑148 (MQ=255)
                                                                                            ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                    >  2:528155/1‑148 (MQ=255)
                                                                                            ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                    >  1:3057925/1‑148 (MQ=255)
                                                                                                 cccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAcc                                               <  1:2961166/148‑1 (MQ=255)
                                                                                                 cccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAcc                                               >  2:2585858/1‑148 (MQ=255)
                                                                                                 cccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAcc                                               <  1:3219907/148‑1 (MQ=255)
                                                                                                  ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCTACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAccc                                              <  1:3550029/148‑1 (MQ=255)
                                                                                                   cGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCa                                             >  2:1619901/1‑148 (MQ=255)
                                                                                                     gATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAgg                                           >  1:3520006/1‑148 (MQ=255)
                                                                                                     gATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAgg                                           <  1:2360543/148‑1 (MQ=255)
                                                                                                       tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                         <  2:1478928/148‑1 (MQ=255)
                                                                                                       tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGATACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                         >  2:226677/1‑148 (MQ=255)
                                                                                                        atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGc                                        <  1:362150/148‑1 (MQ=255)
                                                                                                         tgaagcaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCa                                       <  1:2432039/142‑1 (MQ=255)
                                                                                                          gaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAg                                      >  1:1863868/1‑148 (MQ=255)
                                                                                                             tgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGTGATGGCTCCGCTGACCCAGGAGCAGAAg                                   <  2:2019484/148‑1 (MQ=255)
                                                                                                              gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGc                                  >  2:3456031/1‑148 (MQ=255)
                                                                                                               aCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGcc                                 >  1:417291/1‑148 (MQ=255)
                                                                                                                   gCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGtt                             >  1:1165544/1‑148 (MQ=255)
                                                                                                                   gCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGtt                             >  1:74819/1‑148 (MQ=255)
                                                                                                                        gcgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTaa                        <  2:203382/148‑1 (MQ=255)
                                                                                                                         cgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTaaa                       >  2:674033/1‑148 (MQ=255)
                                                                                                                         catAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTaaa                       >  1:2183334/3‑148 (MQ=255)
                                                                                                                              ccGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGt                  >  2:3356289/1‑148 (MQ=255)
                                                                                                                                 ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAATCCGGTTGCTAAAGTCGTGaa               >  2:3196543/1‑148 (MQ=255)
                                                                                                                                 ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGaa               <  2:725646/148‑1 (MQ=255)
                                                                                                                                    ttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGa            <  1:2346120/148‑1 (MQ=255)
                                                                                                                                    ttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGa            <  1:284113/148‑1 (MQ=255)
                                                                                                                                    ttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGa            <  1:281205/148‑1 (MQ=255)
                                                                                                                                      gCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACa          <  2:773988/148‑1 (MQ=255)
                                                                                                                                         aCAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg       <  2:1021507/148‑1 (MQ=255)
                                                                                                                                         aCAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg       <  2:2780013/148‑1 (MQ=255)
                                                                                                                                            ggTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcgc    <  1:2770471/148‑1 (MQ=255)
                                                                                                                                              tataGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGcgccg  >  1:1877904/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCG  >  CP009273/102591‑102880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑