Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,114,329 C→A 100% L58F (TTG→TTT)  yceJ ← putative cytochrome b561

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,114,3290CA96.3% 569.5 / 12.2 187L58F (TTG→TTT) yceJputative cytochrome b561
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (96/84);  total (100/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 195 total aligned reads displayed.

GCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCA  >  CP009273/1114182‑1114473
                                                                                                                                                   |                                                                                                                                                
gCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAc                                                                                                                                                  >  1:1224800/1‑148 (MQ=255)
   gATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTaaaaaa                                                                                                                                               >  1:2781952/1‑148 (MQ=255)
   gATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTaaaaaa                                                                                                                                               >  1:3097024/1‑148 (MQ=255)
   gATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATAaaaaaa                                                                                                                                               >  1:2482186/1‑148 (MQ=255)
    aTGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAaaaaaa                                                                                                                                              >  1:953936/1‑148 (MQ=255)
    aTGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAaaaaaa                                                                                                                                              >  1:1496448/1‑148 (MQ=255)
      gCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATa                                                                                                                                            <  2:588022/148‑1 (MQ=255)
              cGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCt                                                                                                                                    <  2:2847734/148‑1 (MQ=255)
                aaaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCttt                                                                                                                                  >  1:1978915/1‑148 (MQ=255)
                  aaGCAATAGGTACACTGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTa                                                                                                                                <  1:14137/148‑1 (MQ=255)
                     cAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGc                                                                                                                             <  1:3473301/148‑1 (MQ=255)
                     cAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGc                                                                                                                             <  1:254260/148‑1 (MQ=255)
                        tAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGt                                                                                                                          >  2:415962/1‑148 (MQ=255)
                        tAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGt                                                                                                                          >  1:1041507/1‑148 (MQ=255)
                         aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                         >  2:1293261/1‑148 (MQ=255)
                         aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                         <  2:3010996/148‑1 (MQ=255)
                         aGGTACAATGCGAGATGTCCAGCTATTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCAGGCGGTGGAGATATGACACGCCACAGATCGCGAATAACCATCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                         <  2:262100/148‑1 (MQ=255)
                           gTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCg                                                                                                                       >  2:311117/1‑148 (MQ=255)
                           gTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGAGATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCg                                                                                                                       >  2:3495578/1‑148 (MQ=255)
                            tACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGTACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATTCAGTTCgg                                                                                                                      <  2:1908077/148‑1 (MQ=255)
                            tACAATGCGAGATGTCCAGCTCGTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCgg                                                                                                                      <  2:1841530/148‑1 (MQ=255)
                              cAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGt                                                                                                                    >  2:303701/1‑148 (MQ=255)
                                 tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCt                                                                                                                 >  1:1068544/1‑148 (MQ=255)
                                 tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCt                                                                                                                 <  2:1643788/148‑1 (MQ=255)
                                 tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCt                                                                                                                 <  1:1261978/148‑1 (MQ=255)
                                   cGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCttt                                                                                                               >  2:2325624/1‑148 (MQ=255)
                                    gagaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtg                                                                                                              <  1:1822334/148‑1 (MQ=255)
                                      gaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                            <  2:3351324/148‑1 (MQ=255)
                                      gaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                            >  1:1567505/1‑148 (MQ=255)
                                      gaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                            <  1:1523111/148‑1 (MQ=255)
                                       aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGa                                                                                                           >  1:238995/1‑148 (MQ=255)
                                       aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGa                                                                                                           <  2:614238/148‑1 (MQ=255)
                                       aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGa                                                                                                           >  2:945562/1‑148 (MQ=255)
                                         gTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATa                                                                                                         >  1:3399062/1‑148 (MQ=255)
                                           ccAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAcc                                                                                                       >  2:2587945/1‑148 (MQ=255)
                                            cAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCa                                                                                                      <  1:3095605/148‑1 (MQ=255)
                                            cAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCa                                                                                                      <  2:2372462/148‑1 (MQ=255)
                                              gCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGc                                                                                                    >  2:3300852/1‑148 (MQ=255)
                                               ctctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                   >  1:1531943/1‑148 (MQ=255)
                                               ctctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                   <  2:179896/148‑1 (MQ=255)
                                                 ctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCAt                                                                                                 >  2:3352153/1‑148 (MQ=255)
                                                  ttGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATc                                                                                                >  2:1721759/1‑148 (MQ=255)
                                                       gCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaat                                                                                           >  1:1664139/1‑148 (MQ=255)
                                                        cAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          <  2:507256/148‑1 (MQ=255)
                                                        cAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          >  2:963608/1‑148 (MQ=255)
                                                        cAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          >  2:3263484/1‑148 (MQ=255)
                                                          aGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAAc                                                                                        <  2:135221/148‑1 (MQ=255)
                                                            aCGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGTGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTg                                                                                      >  1:1658256/1‑148 (MQ=255)
                                                               aGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGc                                                                                   >  1:249036/1‑148 (MQ=255)
                                                               aGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGc                                                                                   >  2:2413114/1‑148 (MQ=255)
                                                                  cATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTg                                                                                <  2:1567505/148‑1 (MQ=255)
                                                                           aTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                       >  1:206079/1‑148 (MQ=255)
                                                                               cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGAc                                                                   >  1:552868/1‑148 (MQ=255)
                                                                               cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGAc                                                                   <  2:1899169/148‑1 (MQ=255)
                                                                               cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGAc                                                                   >  2:1054265/1‑148 (MQ=255)
                                                                               cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCAGCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGAc                                                                   >  1:2174827/1‑148 (MQ=255)
                                                                                 cGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCt                                                                 >  1:578839/1‑148 (MQ=255)
                                                                                   gCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTaa                                                               >  1:1672864/1‑148 (MQ=255)
                                                                                    cAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTaaa                                                              >  2:476699/1‑148 (MQ=255)
                                                                                      gCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGc                                                            >  1:530861/1‑148 (MQ=255)
                                                                                      gCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGc                                                            >  1:924929/1‑148 (MQ=255)
                                                                                       cGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCa                                                           >  1:2437062/1‑148 (MQ=255)
                                                                                       cGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCa                                                           >  1:3374067/1‑148 (MQ=255)
                                                                                         gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaaa                                                         <  1:160256/148‑1 (MQ=255)
                                                                                         gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaaa                                                         >  2:3050422/1‑148 (MQ=255)
                                                                                          aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                        <  1:570269/148‑1 (MQ=255)
                                                                                          aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                        <  1:2360117/148‑1 (MQ=255)
                                                                                          aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                        >  1:230684/1‑148 (MQ=255)
                                                                                           cccGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACa                                                       >  2:672039/1‑148 (MQ=255)
                                                                                             cggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATg                                                     <  2:793912/148‑1 (MQ=255)
                                                                                                   tGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                               >  2:2984243/1‑148 (MQ=255)
                                                                                                   tGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                               >  2:380294/1‑148 (MQ=255)
                                                                                                        atatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaat                                          >  2:245250/1‑148 (MQ=255)
                                                                                                        atatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaat                                          <  2:682854/148‑1 (MQ=255)
                                                                                                        atatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaat                                          >  1:771874/1‑148 (MQ=255)
                                                                                                         tatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaata                                         <  2:3152527/148‑1 (MQ=255)
                                                                                                         tatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaata                                         <  1:2186358/148‑1 (MQ=255)
                                                                                                             acacGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTg                                     >  1:1120970/1‑148 (MQ=255)
                                                                                                             acacGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTg                                     >  2:460753/1‑148 (MQ=255)
                                                                                                              cacGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTgc                                    >  2:1870961/1‑148 (MQ=255)
                                                                                                                cGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTgcgc                                  >  2:2100787/1‑148 (MQ=255)
                                                                                                                  ccACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCtt                                <  1:393705/148‑1 (MQ=255)
                                                                                                                     caGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGc                             <  2:3399062/148‑1 (MQ=255)
                                                                                                                     caGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGc                             <  1:3059378/148‑1 (MQ=255)
                                                                                                                       gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCa                           <  2:658289/148‑1 (MQ=255)
                                                                                                                           gcgAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATgg                       >  2:729940/1‑148 (MQ=255)
                                                                                                                              aaTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAg                    >  2:1532931/1‑148 (MQ=255)
                                                                                                                                tAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGc                  >  2:2141117/1‑148 (MQ=255)
                                                                                                                                 aaCCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGcc                 >  2:2711667/1‑148 (MQ=255)
                                                                                                                                   ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGc               >  2:3187455/1‑148 (MQ=255)
                                                                                                                                   ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGc               >  2:367456/1‑148 (MQ=255)
                                                                                                                                       ccccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGat           >  1:2732416/1‑148 (MQ=255)
                                                                                                                                       ccccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGat           >  2:2191453/1‑148 (MQ=255)
                                                                                                                                         ccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGatat         <  2:2153600/148‑1 (MQ=255)
                                                                                                                                         ccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGatat         >  1:1816428/1‑148 (MQ=255)
                                                                                                                                             catTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAaca     <  1:1765468/148‑1 (MQ=255)
                                                                                                                                             catTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCAAATGGAAGGCCGCAGATATAaca     <  2:530900/148‑1 (MQ=255)
                                                                                                                                               ttAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAcc   >  1:345725/1‑148 (MQ=255)
                                                                                                                                                tAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCa  <  2:1796369/148‑1 (MQ=255)
                                                                                                                                                tAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCa  >  1:1782574/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCA  >  CP009273/1114182‑1114473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑