Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,994 C→A 100% R245S (CGC→AGC)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,9940CA95.6% 535.1 / 15.6 182R245S (CGC→AGC) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (89/85);  total (94/88)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 185 total aligned reads displayed.

GTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGC  >  CP009273/1122849‑1123141
                                                                                                                                                 |                                                                                                                                                   
gTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAgagc                                                                                                                                                   >  1:3082395/1‑148 (MQ=255)
   tGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCggg                                                                                                                                                <  1:1857508/148‑1 (MQ=255)
    gCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGc                                                                                                                                               <  2:1281837/148‑1 (MQ=255)
    gCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGc                                                                                                                                               <  2:3247816/148‑1 (MQ=255)
    gCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGc                                                                                                                                               <  2:2798084/148‑1 (MQ=255)
     ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCa                                                                                                                                              >  2:3383756/1‑148 (MQ=255)
       gAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCCgg                                                                                                                                            >  2:1427969/1‑148 (MQ=255)
       gAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAgg                                                                                                                                            <  2:2011978/148‑1 (MQ=255)
       gAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAgg                                                                                                                                            >  2:1427130/1‑148 (MQ=255)
        aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAggg                                                                                                                                           >  2:2526690/1‑148 (MQ=255)
         gCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGc                                                                                                                                          >  1:2616184/1‑148 (MQ=255)
              aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGt                                                                                                                                     >  2:2713547/1‑148 (MQ=255)
               tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTg                                                                                                                                    >  1:1408387/1‑148 (MQ=255)
               tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTg                                                                                                                                    >  1:394222/1‑148 (MQ=255)
               tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTg                                                                                                                                    <  1:2163595/148‑1 (MQ=255)
                ttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                                   <  2:348173/148‑1 (MQ=255)
                      gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                             >  1:843707/1‑148 (MQ=255)
                      gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                             <  1:3475677/148‑1 (MQ=255)
                       cTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAcc                                                                                                                            <  1:2405377/148‑1 (MQ=255)
                          gTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGAt                                                                                                                         <  2:2495514/148‑1 (MQ=255)
                            ccTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                       <  1:1037072/148‑1 (MQ=255)
                            ccTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                       <  1:2786708/148‑1 (MQ=255)
                             cTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTcc                                                                                                                      <  1:368250/148‑1 (MQ=255)
                              tttGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTTACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCt                                                                                                                     >  2:2242120/1‑148 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                                   <  1:750278/148‑1 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                                   <  2:204468/148‑1 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                                   <  1:2539032/148‑1 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                                   <  2:326211/148‑1 (MQ=255)
                                  cAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGtt                                                                                                                 <  2:1165074/148‑1 (MQ=255)
                                   aGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTg                                                                                                                >  2:1036657/1‑148 (MQ=255)
                                    gATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTgg                                                                                                               >  1:3025192/1‑148 (MQ=255)
                                     aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGc                                                                                                              >  2:2051863/1‑148 (MQ=255)
                                     aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGc                                                                                                              >  1:3336787/1‑148 (MQ=255)
                                     aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGc                                                                                                              >  2:2494478/1‑148 (MQ=255)
                                      tCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCa                                                                                                             >  2:3079033/1‑148 (MQ=255)
                                       caccacCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCag                                                                                                            >  2:3339386/1‑148 (MQ=255)
                                        accaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCaga                                                                                                           >  1:1158995/1‑148 (MQ=255)
                                        accaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCaga                                                                                                           >  2:936472/1‑148 (MQ=255)
                                        accaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCaga                                                                                                           <  1:1254094/148‑1 (MQ=255)
                                        accaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCaga                                                                                                           >  1:1702589/1‑148 (MQ=255)
                                         ccaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCagag                                                                                                          >  1:855791/1‑148 (MQ=255)
                                         ccaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCagag                                                                                                          <  1:2051848/148‑1 (MQ=255)
                                             cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATCCCTGGTTGGCAGAGTACg                                                                                                      <  1:1313404/148‑1 (MQ=255)
                                             cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGAGTCCTGGTTGGCAGAGTACg                                                                                                      <  2:1133485/148‑1 (MQ=255)
                                              tGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGc                                                                                                     <  2:1495263/148‑1 (MQ=255)
                                                      tCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCaa                                                                                             <  2:1253881/148‑1 (MQ=255)
                                                       cGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAc                                                                                            >  1:305806/1‑148 (MQ=255)
                                                       cGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAc                                                                                            >  1:305886/1‑148 (MQ=255)
                                                         ccGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACTGATATGCGCGAATGGCTTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGt                                                                                          >  1:299373/1‑148 (MQ=255)
                                                         ccGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGt                                                                                          >  2:1852398/1‑148 (MQ=255)
                                                            tGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTggg                                                                                       >  1:436522/1‑148 (MQ=255)
                                                             gCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGt                                                                                      <  1:3201484/148‑1 (MQ=255)
                                                             gCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGt                                                                                      <  1:90729/148‑1 (MQ=255)
                                                              ccGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGtt                                                                                     >  2:1220798/1‑148 (MQ=255)
                                                                   aGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCg                                                                                >  2:1347181/1‑148 (MQ=255)
                                                                     tCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGc                                                                              <  2:1953972/148‑1 (MQ=255)
                                                                     tCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGc                                                                              <  2:1953976/148‑1 (MQ=255)
                                                                         cGTGAACCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgtg                                                                          <  2:3144513/148‑1 (MQ=255)
                                                                         cGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCACCGTGGGTTTGTCGGCtgtg                                                                          >  2:2402837/1‑148 (MQ=255)
                                                                         cGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgtg                                                                          <  2:246885/148‑1 (MQ=255)
                                                                           tGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTgcg                                                                        <  2:423505/148‑1 (MQ=255)
                                                                           tGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTgcg                                                                        <  2:423495/148‑1 (MQ=255)
                                                                              aaCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCgg                                                                     <  1:3492399/148‑1 (MQ=255)
                                                                                   tGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACtt                                                                <  1:781259/148‑1 (MQ=255)
                                                                                   tGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACtt                                                                >  2:3507817/1‑148 (MQ=255)
                                                                                    gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTc                                                               <  2:3236813/148‑1 (MQ=255)
                                                                                    gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTc                                                               <  1:145282/148‑1 (MQ=255)
                                                                                         ccGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGa                                                          <  2:1634298/148‑1 (MQ=255)
                                                                                         ccGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGa                                                          <  1:165195/148‑1 (MQ=255)
                                                                                           gTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAt                                                        <  1:57025/148‑1 (MQ=255)
                                                                                             gactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgt                                                      >  2:2832064/1‑148 (MQ=255)
                                                                                                       gcgcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAcc                                            <  2:130532/148‑1 (MQ=255)
                                                                                                         gcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAg                                          <  2:1877371/148‑1 (MQ=255)
                                                                                                          cTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGa                                         <  2:2115996/148‑1 (MQ=255)
                                                                                                            cATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACa                                       >  2:2790491/1‑148 (MQ=255)
                                                                                                              tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                     <  1:2918951/148‑1 (MQ=255)
                                                                                                              tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                     <  1:962034/148‑1 (MQ=255)
                                                                                                               cGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGtt                                    <  2:2270217/148‑1 (MQ=255)
                                                                                                                gACATTACGGATATGCGCGACTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTc                                   <  1:2267609/148‑1 (MQ=255)
                                                                                                                gACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTc                                   <  1:182262/148‑1 (MQ=255)
                                                                                                                gACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTc                                   <  2:3490366/148‑1 (MQ=255)
                                                                                                                  cATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCg                                 >  1:2691416/1‑148 (MQ=255)
                                                                                                                   aTTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGc                                >  1:1026563/1‑148 (MQ=255)
                                                                                                                   aTTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGc                                >  1:1026442/1‑148 (MQ=255)
                                                                                                                     tACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaa                              >  1:558483/1‑148 (MQ=255)
                                                                                                                      aCGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaaa                             >  2:1364353/1‑148 (MQ=255)
                                                                                                                          atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                         <  1:2096417/148‑1 (MQ=255)
                                                                                                                             tGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                      >  2:976794/1‑148 (MQ=255)
                                                                                                                              gcgcGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGc                     >  2:2482396/1‑148 (MQ=255)
                                                                                                                                   aaTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACa                >  1:74292/1‑148 (MQ=255)
                                                                                                                                    aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg               >  2:1874651/1‑148 (MQ=255)
                                                                                                                                     tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg              >  2:3027357/1‑148 (MQ=255)
                                                                                                                                      ggCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGc             >  2:3459125/1‑148 (MQ=255)
                                                                                                                                      ggCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGc             >  1:2614065/1‑148 (MQ=255)
                                                                                                                                        cGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCg           <  2:766336/148‑1 (MQ=255)
                                                                                                                                          tGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTg         >  2:2612303/1‑148 (MQ=255)
                                                                                                                                           gaggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGc        >  2:2223972/1‑148 (MQ=255)
                                                                                                                                            aggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt       >  2:2684054/1‑148 (MQ=255)
                                                                                                                                            aggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt       >  2:2684077/1‑148 (MQ=255)
                                                                                                                                            aggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt       <  2:3082395/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGC  >  CP009273/1122849‑1123141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑