Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,181,504 G→T 100% D69Y (GAT→TAT)  pepT → peptidase T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,181,5040GT97.9% 751.1 / 5.7 235D69Y (GAT→TAT) pepTpeptidase T
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (105/125);  total (107/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

GGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGT  >  CP009273/1181361‑1181648
                                                                                                                                               |                                                                                                                                                
gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              >  2:2667798/1‑148 (MQ=255)
gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              >  1:259696/1‑148 (MQ=255)
gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              <  2:3097495/148‑1 (MQ=255)
gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              <  2:650831/148‑1 (MQ=255)
  gTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATcc                                                                                                                                            >  2:1717397/1‑148 (MQ=255)
   tGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATccc                                                                                                                                           <  1:1434801/148‑1 (MQ=255)
        cAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGa                                                                                                                                      <  2:1371150/148‑1 (MQ=255)
        cAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGa                                                                                                                                      <  2:1675841/148‑1 (MQ=255)
         aGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGAt                                                                                                                                     <  1:296545/148‑1 (MQ=255)
           gTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTg                                                                                                                                   <  2:2750127/148‑1 (MQ=255)
           gTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTGATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTg                                                                                                                                   <  2:3214064/148‑1 (MQ=255)
              cccAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCt                                                                                                                                <  1:1058857/148‑1 (MQ=255)
              cccAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCt                                                                                                                                <  1:1475810/148‑1 (MQ=255)
               ccAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCtt                                                                                                                               >  1:1262558/1‑148 (MQ=255)
                cAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCttt                                                                                                                              >  1:3006981/1‑148 (MQ=255)
                  gCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAt                                                                                                                            >  1:3474489/1‑148 (MQ=255)
                       gAAGGCCAATGGAAGTTATTGCATCTGCTGTAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtctc                                                                                                                       <  2:2474377/148‑1 (MQ=255)
                         aGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAt                                                                                                                     >  2:1916967/1‑148 (MQ=255)
                          ggCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAtg                                                                                                                    >  1:556224/1‑148 (MQ=255)
                          ggCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAtg                                                                                                                    >  2:3171811/1‑148 (MQ=255)
                          ggCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAtg                                                                                                                    <  2:1036139/148‑1 (MQ=255)
                             cAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTgg                                                                                                                 <  1:293347/148‑1 (MQ=255)
                              aaTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGa                                                                                                                >  2:218882/1‑148 (MQ=255)
                               aTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAt                                                                                                               >  2:2221770/1‑148 (MQ=255)
                               aTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAt                                                                                                               >  2:677021/1‑148 (MQ=255)
                                 ggAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATAc                                                                                                             >  2:1425412/1‑148 (MQ=255)
                                   aaGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCt                                                                                                           <  1:406580/148‑1 (MQ=255)
                                     gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCa                                                                                                         >  2:1547760/1‑148 (MQ=255)
                                      ttATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAc                                                                                                        >  1:1175344/1‑148 (MQ=255)
                                       tATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                       <  1:1083363/148‑1 (MQ=255)
                                       tATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                       <  2:2354780/148‑1 (MQ=255)
                                         ttGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCgg                                                                                                     <  1:2898058/148‑1 (MQ=255)
                                         ttGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCgg                                                                                                     >  2:2085381/1‑148 (MQ=255)
                                          tGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGa                                                                                                    <  1:476842/148‑1 (MQ=255)
                                          tGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGa                                                                                                    <  2:2923814/148‑1 (MQ=255)
                                              tCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGc                                                                                                >  1:244012/1‑148 (MQ=255)
                                               ctgctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCa                                                                                               >  1:3206605/1‑148 (MQ=255)
                                                tgctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAg                                                                                              <  1:2008027/148‑1 (MQ=255)
                                                   tgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCgg                                                                                           <  1:1017212/148‑1 (MQ=255)
                                                      aaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaa                                                                                        <  1:2699680/148‑1 (MQ=255)
                                                        gagCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaa                                                                                      <  1:425094/148‑1 (MQ=255)
                                                          gcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCacacat                                                                                    >  2:3383868/1‑143 (MQ=255)
                                                          gcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAt                                                                                    >  2:1943836/1‑148 (MQ=255)
                                                           cagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtg                                                                                   >  1:88537/1‑148 (MQ=255)
                                                           cagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtg                                                                                   >  1:2827203/1‑148 (MQ=255)
                                                              cTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATTTGa                                                                                >  1:1916415/1‑148 (MQ=255)
                                                              cTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGa                                                                                >  1:523302/1‑148 (MQ=255)
                                                               tCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGaa                                                                               <  2:870628/148‑1 (MQ=255)
                                                               tCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGaa                                                                               <  1:2806658/148‑1 (MQ=255)
                                                                 gAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATc                                                                             <  2:385054/148‑1 (MQ=255)
                                                                 gAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATc                                                                             <  2:156541/148‑1 (MQ=255)
                                                                     agaTGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCa                                                                         >  2:2912848/1‑148 (MQ=255)
                                                                        tGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAt                                                                      <  1:1820709/148‑1 (MQ=255)
                                                                          gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    <  2:62371/148‑1 (MQ=255)
                                                                          gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    <  2:1413978/148‑1 (MQ=255)
                                                                            gCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTTCAGCGGCAAAAATGTGAATCCGCACAttgtt                                                                  >  1:2694025/1‑148 (MQ=255)
                                                                             cTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttgttg                                                                 >  1:3079293/1‑148 (MQ=255)
                                                                              ttATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGa                                                                <  1:15001/148‑1 (MQ=255)
                                                                               tATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCACAAATGTGAATCCGCAAATTGTTGaa                                                               >  2:91629/1‑148 (MQ=255)
                                                                               tATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaa                                                               >  1:3446210/1‑148 (MQ=255)
                                                                                aTCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaa                                                              <  2:1805761/148‑1 (MQ=255)
                                                                                aTCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaa                                                              <  2:49102/148‑1 (MQ=255)
                                                                                 tCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaaa                                                             <  1:2878188/148‑1 (MQ=255)
                                                                                  cAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCTAAAATGTGAATCCGCAAATTGTTGAAAAc                                                            <  1:3059445/148‑1 (MQ=255)
                                                                                  cAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCTCAAATTGTTGaaaaa                                                            >  2:769680/1‑147 (MQ=255)
                                                                                  cAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAAc                                                            <  1:688467/148‑1 (MQ=255)
                                                                                  cAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAAc                                                            <  1:3370326/148‑1 (MQ=255)
                                                                                    aTGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTa                                                          <  1:218882/148‑1 (MQ=255)
                                                                                     tgtgACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                         >  1:397075/1‑148 (MQ=255)
                                                                                         aCCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATcgcg                                                     <  2:3519317/148‑1 (MQ=255)
                                                                                           cTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGt                                                   <  1:1784237/148‑1 (MQ=255)
                                                                                             tAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTgg                                                 >  1:3399099/1‑148 (MQ=255)
                                                                                               aGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCg                                               >  2:1099338/1‑148 (MQ=255)
                                                                                                 tGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGat                                             <  1:1352570/148‑1 (MQ=255)
                                                                                                 tGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGat                                             <  1:781626/148‑1 (MQ=255)
                                                                                                  gagaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGata                                            <  2:775198/148‑1 (MQ=255)
                                                                                                   agaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGatat                                           <  2:244012/148‑1 (MQ=255)
                                                                                                    gaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTAACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTTAAAACTATCGCGGTGGCGATAtt                                          >  1:149295/1‑148 (MQ=255)
                                                                                                       gggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTg                                         >  2:2994188/1‑146 (MQ=255)
                                                                                                        ggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgcgc                                      >  2:715731/1‑148 (MQ=255)
                                                                                                         gCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCt                                     >  2:3055897/1‑148 (MQ=255)
                                                                                                           aCTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTgg                                   >  2:798947/1‑148 (MQ=255)
                                                                                                            cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                  <  2:1831417/148‑1 (MQ=255)
                                                                                                             tttGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAGTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGt                                 >  1:2053456/1‑148 (MQ=255)
                                                                                                              ttGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTa                                >  2:812742/1‑148 (MQ=255)
                                                                                                              ttGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTa                                >  2:814556/1‑148 (MQ=255)
                                                                                                                 aTGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCg                             <  2:1175344/148‑1 (MQ=255)
                                                                                                                            aCCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGtt                  <  1:2121213/148‑1 (MQ=255)
                                                                                                                            aCCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGtt                  <  2:405765/148‑1 (MQ=255)
                                                                                                                             ccGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGttt                 >  1:245141/1‑148 (MQ=255)
                                                                                                                                    aCGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAcc          >  2:434589/1‑148 (MQ=255)
                                                                                                                                    aCGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAcc          <  1:1917151/148‑1 (MQ=255)
                                                                                                                                    aCGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAcc          >  2:2586086/1‑148 (MQ=255)
                                                                                                                                      gTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCgg        <  1:2600310/148‑1 (MQ=255)
                                                                                                                                        cccTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGtt      <  1:1711993/148‑1 (MQ=255)
                                                                                                                                        cccTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGtt      >  1:2662346/1‑148 (MQ=255)
                                                                                                                                           tGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATg   <  1:3278752/148‑1 (MQ=255)
                                                                                                                                            ggCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGt  >  1:188832/1‑148 (MQ=255)
                                                                                                                                            ggCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGt  <  2:3007169/148‑1 (MQ=255)
                                                                                                                                            ggCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGt  <  1:1453414/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGT  >  CP009273/1181361‑1181648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑