Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,257,232 G→T 100% R34S (CGC→AGC)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,257,2320GT97.4% 631.4 / 6.2 206R34S (CGC→AGC) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (110/90);  total (114/92)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 207 total aligned reads displayed.

GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCC  >  CP009273/1257087‑1257375
                                                                                                                                                 |                                                                                                                                               
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                               <  2:1553100/148‑1 (MQ=255)
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                               <  2:1553051/148‑1 (MQ=255)
   gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTa                                                                                                                                            <  1:1475044/148‑1 (MQ=255)
    aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGctacctac                                                                                                                                           <  2:239246/148‑1 (MQ=255)
     aGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                          <  1:2108608/148‑1 (MQ=255)
     aGCACGACGCAGCGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                          <  2:3202006/148‑1 (MQ=255)
      gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                         >  1:1171571/1‑148 (MQ=255)
      gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                         >  2:522597/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                      >  1:2979471/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                      >  1:2755254/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAgcgg                                                                                                                                      >  1:265600/1‑148 (MQ=255)
           acgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcggcg                                                                                                                                    >  2:2026220/1‑148 (MQ=255)
              cAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                 >  1:1597829/1‑148 (MQ=255)
                gggCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGcc                                                                                                                               >  2:1968371/1‑148 (MQ=255)
                   cATCAACCATAACGACTACTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAg                                                                                                                            <  2:2558419/148‑1 (MQ=255)
                   cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAg                                                                                                                            >  2:3228975/1‑148 (MQ=255)
                      cAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGa                                                                                                                         <  2:1295979/148‑1 (MQ=255)
                          cATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                     <  1:192608/148‑1 (MQ=255)
                          cATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                     >  2:2854850/1‑148 (MQ=255)
                               cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                >  1:19658/1‑148 (MQ=255)
                                 aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGc                                                                                                              >  2:473773/1‑148 (MQ=255)
                                  cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                             <  2:182303/148‑1 (MQ=255)
                                   tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                            <  1:208552/148‑1 (MQ=255)
                                   tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                            >  1:3473680/1‑148 (MQ=255)
                                     aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                          <  2:1341944/148‑1 (MQ=255)
                                     aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTAATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                          >  2:39959/1‑148 (MQ=255)
                                       tCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                        <  2:3287023/148‑1 (MQ=255)
                                         cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                      <  2:2787943/148‑1 (MQ=255)
                                         cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                      <  2:332075/148‑1 (MQ=255)
                                            cAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATa                                                                                                   <  1:891623/148‑1 (MQ=255)
                                                     gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCt                                                                                          <  1:3358860/148‑1 (MQ=255)
                                                      ttAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTa                                                                                         <  1:489939/148‑1 (MQ=255)
                                                      ttAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTa                                                                                         <  1:1115246/148‑1 (MQ=255)
                                                       tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                        <  2:2966932/148‑1 (MQ=255)
                                                         gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGtt                                                                                      <  2:1771703/148‑1 (MQ=255)
                                                         gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGtt                                                                                      <  2:801844/148‑1 (MQ=255)
                                                          tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTc                                                                                     <  1:1742422/148‑1 (MQ=255)
                                                            ggggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                                   <  1:1913131/148‑1 (MQ=255)
                                                                 acaAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                              >  2:2624236/1‑148 (MQ=255)
                                                                   aaGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGc                                                                            >  1:1458887/1‑148 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCACTACGTTGTGCTAGTTCCGGGGTGGCg                                                                           >  1:3370778/1‑148 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                           >  1:1952522/1‑148 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTCCCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       >  2:698401/1‑148 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       <  1:257450/148‑1 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       <  2:2755254/148‑1 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       >  2:1038754/1‑148 (MQ=255)
                                                                           tGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                    >  1:3481632/1‑148 (MQ=255)
                                                                                 atgaAAATATCACCACCGCGTACATTTTCAGTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaaaa                                                              >  1:2452360/1‑148 (MQ=255)
                                                                                  tgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                             >  2:737600/1‑148 (MQ=255)
                                                                                    aaaaTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCt                                                           >  2:110602/1‑148 (MQ=255)
                                                                                      aaTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTc                                                         <  1:1244622/148‑1 (MQ=255)
                                                                                       atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCa                                                        >  2:2808417/1‑148 (MQ=255)
                                                                                        tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCat                                                       <  1:51685/148‑1 (MQ=255)
                                                                                        tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCat                                                       >  2:2070185/1‑148 (MQ=255)
                                                                                         atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                      >  2:3547225/1‑148 (MQ=255)
                                                                                          tCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCatat                                                     <  2:3501849/148‑1 (MQ=255)
                                                                                           caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                    >  2:175856/1‑148 (MQ=255)
                                                                                            accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa                                                   >  2:1535705/1‑148 (MQ=255)
                                                                                            accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGAGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa                                                   <  1:2232422/148‑1 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  1:235581/1‑148 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  1:1020094/1‑148 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  2:264292/1‑148 (MQ=255)
                                                                                              caccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAgg                                                 >  1:728666/1‑148 (MQ=255)
                                                                                                 cgcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                              >  1:2601450/1‑148 (MQ=255)
                                                                                                 cgcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                              <  1:262361/148‑1 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGTTTGGCAATACGTTGTGCTAGTTCAGGGGTGGCGATACCAGCAAAAAGCTTCATATCAGGCACgaga                                          >  2:823929/1‑148 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                          <  2:2758290/148‑1 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                          >  2:270717/1‑148 (MQ=255)
                                                                                                        aTTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaaga                                       <  1:2291950/148‑1 (MQ=255)
                                                                                                         ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAgaagaa                                      >  2:559872/1‑148 (MQ=255)
                                                                                                         ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAgaagaa                                      <  2:105891/148‑1 (MQ=255)
                                                                                                          tttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAc                                     >  2:3136416/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:684494/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:24700/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:684585/1‑148 (MQ=255)
                                                                                                                 aaTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                              >  2:1347242/1‑148 (MQ=255)
                                                                                                                 aaTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                              <  1:2854850/148‑1 (MQ=255)
                                                                                                                   tttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAt                            >  2:1821306/1‑148 (MQ=255)
                                                                                                                    ttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                           >  1:2169482/1‑148 (MQ=255)
                                                                                                                       tACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGt                        >  1:3452804/1‑148 (MQ=255)
                                                                                                                       tACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGt                        >  1:3452259/1‑148 (MQ=255)
                                                                                                                         cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTcc                      <  2:3342770/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:1347229/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:1347290/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     >  1:2328946/1‑148 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:892881/148‑1 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  1:2437866/1‑148 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  1:648477/1‑148 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  2:2294522/1‑148 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa         >  2:2847444/1‑148 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa         <  1:926933/148‑1 (MQ=255)
                                                                                                                                       tCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa        <  1:3438998/148‑1 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       <  1:3134090/148‑1 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       >  2:3053638/1‑148 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       >  2:2891961/1‑148 (MQ=255)
                                                                                                                                         gCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATTCGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATc      <  1:1866958/148‑1 (MQ=255)
                                                                                                                                         gCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATc      <  1:2765371/148‑1 (MQ=255)
                                                                                                                                           tAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTg    >  1:2460200/1‑148 (MQ=255)
                                                                                                                                            aaaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGc   <  1:2846257/148‑1 (MQ=255)
                                                                                                                                             aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGcc  >  2:1922690/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCC  >  CP009273/1257087‑1257375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑