Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,260,354 A→T 30.3% S395S (TCA→TCT)  hemA → glutamyl tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,260,3540AT30.3% 346.8 / 212.1 231S395S (TCA→TCT) hemAglutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (71/90);  new base T (26/44);  total (97/134)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 236 total aligned reads displayed.

CGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCC  >  CP009273/1260207‑1260501
                                                                                                                                                   |                                                                                                                                                   
 gAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCAc                                                                                                                                                    <  2:2722798/148‑1 (MQ=255)
 gAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCAc                                                                                                                                                    <  2:3332160/148‑1 (MQ=255)
 gAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCAc                                                                                                                                                    <  2:3332856/148‑1 (MQ=255)
   ttATCGCAGAAAGGCTGAGCTAGTTAGAGATGTGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCTGGGAGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAAGAGCTTTATCCATGCGCCTACGAAATCACtt                                                                                                                                                  <  2:1186314/147‑1 (MQ=255)
   gTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACtt                                                                                                                                                  >  2:908264/1‑148 (MQ=255)
    tATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTc                                                                                                                                                 >  2:1592495/1‑148 (MQ=255)
    tATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTc                                                                                                                                                 >  1:3026753/1‑148 (MQ=255)
     aTCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCa                                                                                                                                                <  1:1330585/148‑1 (MQ=255)
     aTCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCa                                                                                                                                                <  2:1723014/148‑1 (MQ=255)
         cAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAg                                                                                                                                            <  1:2823905/148‑1 (MQ=255)
          aGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAgg                                                                                                                                           <  2:1263504/148‑1 (MQ=255)
           gCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGgc                                                                                                                                          >  1:2941131/1‑148 (MQ=255)
            ccAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgcc                                                                                                                                         >  2:1775675/1‑148 (MQ=255)
              aGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGACATCACTTCAACAGgccgc                                                                                                                                       >  2:2869789/1‑148 (MQ=255)
               ggCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgccgcc                                                                                                                                      <  1:1598414/148‑1 (MQ=255)
               ggCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgccgcc                                                                                                                                      <  1:3298087/148‑1 (MQ=255)
                gCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGccc                                                                                                                                     <  2:669011/148‑1 (MQ=255)
                gCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGACCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGccc                                                                                                                                     >  2:1410171/1‑148 (MQ=255)
                   gagCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTg                                                                                                                                  <  1:805095/148‑1 (MQ=255)
                    agCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGa                                                                                                                                 <  2:1902753/148‑1 (MQ=255)
                         gTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACgggg                                                                                                                            <  1:131284/148‑1 (MQ=255)
                          ttCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGa                                                                                                                           >  2:2178779/1‑148 (MQ=255)
                          ttCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGa                                                                                                                           <  2:870646/148‑1 (MQ=255)
                          ttCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCGTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGa                                                                                                                           >  2:2612490/1‑148 (MQ=255)
                           tCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGAt                                                                                                                          >  1:1260713/1‑148 (MQ=255)
                             gcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaa                                                                                                                        <  1:1213492/148‑1 (MQ=255)
                             gcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaa                                                                                                                        <  2:629154/148‑1 (MQ=255)
                                aTGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATaacga                                                                                                                     <  2:2779868/148‑1 (MQ=255)
                                aTGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacga                                                                                                                     <  1:2851684/148‑1 (MQ=255)
                                     ttAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGcc                                                                                                                >  2:2798907/1‑148 (MQ=255)
                                         ccGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGaa                                                                                                            >  2:2540080/1‑148 (MQ=255)
                                          cGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAat                                                                                                           <  1:2732159/148‑1 (MQ=255)
                                             cAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATAtt                                                                                                        <  2:1959163/148‑1 (MQ=255)
                                               aaGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCt                                                                                                      >  1:2493008/1‑148 (MQ=255)
                                                             cTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCg                                                                                        <  1:1908950/148‑1 (MQ=255)
                                                             cTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCg                                                                                        >  1:1481591/1‑148 (MQ=255)
                                                                 aGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCt                                                                                    <  1:2158566/148‑1 (MQ=255)
                                                                  gCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTg                                                                                   >  1:2955394/1‑148 (MQ=255)
                                                                   cAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTgg                                                                                  <  1:3207422/148‑1 (MQ=255)
                                                                      ggcggcGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGt                                                                               <  2:2053101/148‑1 (MQ=255)
                                                                        cggcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAg                                                                             <  2:427200/148‑1 (MQ=255)
                                                                        cggcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAg                                                                             <  2:2534493/148‑1 (MQ=255)
                                                                         ggcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGc                                                                            <  2:3506653/148‑1 (MQ=255)
                                                                         ggcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCNCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGc                                                                            <  1:1808673/148‑1 (MQ=255)
                                                                          gcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCa                                                                           <  1:1294519/148‑1 (MQ=255)
                                                                             aCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTa                                                                        >  1:875549/1‑148 (MQ=255)
                                                                             aCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTa                                                                        <  1:1855331/148‑1 (MQ=255)
                                                                              cgcgCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAc                                                                       <  1:2759449/148‑1 (MQ=255)
                                                                                 gcAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCACCAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcatc                                                                    <  1:1914828/148‑1 (MQ=255)
                                                                                    aGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtt                                                                 >  2:3456189/1‑148 (MQ=255)
                                                                                    aGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtt                                                                 >  1:2653121/1‑148 (MQ=255)
                                                                                     gCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                                                                <  1:2673792/148‑1 (MQ=255)
                                                                                     gCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                                                                >  2:3040906/1‑148 (MQ=255)
                                                                                      ccATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtttt                                                               <  1:3453075/148‑1 (MQ=255)
                                                                                         ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                            >  1:1366973/1‑148 (MQ=255)
                                                                                         ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                            <  1:798765/148‑1 (MQ=255)
                                                                                          tATGCAGGATCTGGCATGGAATCTGACTAACCTCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttt                                                           <  1:1102914/148‑1 (MQ=255)
                                                                                          tATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttt                                                           >  2:2302763/1‑148 (MQ=255)
                                                                                              cAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCCTTTTCttttttt                                                       >  2:1926597/1‑148 (MQ=255)
                                                                                              cAGGATCTGGATTGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCAATTCAACTGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                                                       <  2:2980862/148‑1 (MQ=255)
                                                                                                 gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa                                                    <  1:1422026/148‑1 (MQ=255)
                                                                                                   tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAgg                                                  <  1:2762834/148‑1 (MQ=255)
                                                                                                   tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTGTTACAgg                                                  >  2:152390/1‑148 (MQ=255)
                                                                                                     tGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATAATTTTCTTTTTTTACAGGGt                                                <  1:612863/148‑1 (MQ=255)
                                                                                                       gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGc                                              <  2:1253033/148‑1 (MQ=255)
                                                                                                       gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGc                                              >  1:1494085/1‑148 (MQ=255)
                                                                                                       gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGc                                              <  2:3010242/148‑1 (MQ=255)
                                                                                                       gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGc                                              <  2:2603028/148‑1 (MQ=255)
                                                                                                         aTGGAAACTGCCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACGGGCCGCCGGTGACGGGTATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAt                                            <  1:2020532/148‑1 (MQ=255)
                                                                                                           ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAttt                                          <  1:3116381/148‑1 (MQ=255)
                                                                                                           ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAttt                                          >  2:2448931/1‑148 (MQ=255)
                                                                                                           ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAttt                                          <  2:1008695/148‑1 (MQ=255)
                                                                                                                cTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGcc                                     >  2:957149/1‑148 (MQ=255)
                                                                                                                cTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTGTCTTTTTTTACGGGGTGGATGTAGGcc                                     >  2:3414354/1‑148 (MQ=255)
                                                                                                                        ccGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGc                             >  1:3001579/1‑148 (MQ=255)
                                                                                                                          gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCt                           <  2:938219/148‑1 (MQ=255)
                                                                                                                           cTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCtt                          <  1:3355483/148‑1 (MQ=255)
                                                                                                                           cTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCtt                          >  2:529739/1‑148 (MQ=255)
                                                                                                                              gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTa                       <  1:3485411/148‑1 (MQ=255)
                                                                                                                                 ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCg                    <  2:935904/148‑1 (MQ=255)
                                                                                                                                  cATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                   <  1:428051/148‑1 (MQ=255)
                                                                                                                                  cATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                   >  1:3495383/1‑148 (MQ=255)
                                                                                                                                  cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                   <  2:1574106/148‑1 (MQ=255)
                                                                                                                                   aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGtt                  >  1:2536730/1‑148 (MQ=255)
                                                                                                                                        ccAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaa             <  1:843295/148‑1 (MQ=255)
                                                                                                                                         cAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa            >  1:2786691/1‑148 (MQ=255)
                                                                                                                                         cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa            >  1:2816443/1‑148 (MQ=255)
                                                                                                                                         cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa            >  1:1036789/1‑148 (MQ=255)
                                                                                                                                          aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAAc           >  1:1870230/1‑148 (MQ=255)
                                                                                                                                          aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAAc           >  1:2863926/1‑148 (MQ=255)
                                                                                                                                           aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACt          >  2:316143/1‑148 (MQ=255)
                                                                                                                                               aaTCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGaa      >  1:291750/1‑148 (MQ=255)
                                                                                                                                               aaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGac      >  2:1027635/1‑147 (MQ=255)
                                                                                                                                               aaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGaa      >  1:2708078/1‑148 (MQ=255)
                                                                                                                                                 tCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGc    <  2:1288959/148‑1 (MQ=255)
                                                                                                                                                 tCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCAGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGACTTCTATCGTTGCCAAACTGGAAGc    >  2:1431252/1‑148 (MQ=255)
                                                                                                                                                  cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc   >  1:2075239/1‑148 (MQ=255)
                                                                                                                                                  cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc   >  1:233834/1‑148 (MQ=255)
                                                                                                                                                  cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc   >  1:3092082/1‑148 (MQ=255)
                                                                                                                                                   aCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGccc  >  2:33740/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCC  >  CP009273/1260207‑1260501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑