| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,351,022 | G→T | 100% | R116S (CGT→AGT) | sapA ← | antimicrobial peptide transport ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,351,022 | 0 | G | T | 97.1% | 625.4 / 9.1 | 210 | R116S (CGT→AGT) | sapA | antimicrobial peptide transport ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base T (108/96); total (110/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 210 total aligned reads displayed.
CCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGT > CP009273/1350878‑1351164 | ccAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGt > 2:3343708/1‑148 (MQ=255) aGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTgg > 2:1481565/1‑148 (MQ=255) aGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTgg > 1:3042844/1‑148 (MQ=255) gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTggg < 1:69810/148‑1 (MQ=255) gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATAGTCGGCATTCATTTTACTAGTggg > 2:2858449/1‑148 (MQ=255) tttGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGa < 2:441621/148‑1 (MQ=255) tGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGt < 1:3233430/148‑1 (MQ=255) gACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAc > 2:811184/1‑148 (MQ=255) gACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAc < 2:767295/148‑1 (MQ=255) gACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAAc > 2:828028/1‑148 (MQ=255) tcTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAg > 2:1441587/2‑148 (MQ=255) gCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAg < 1:1832953/148‑1 (MQ=255) tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg < 2:131856/148‑1 (MQ=255) tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg < 2:2678451/148‑1 (MQ=255) tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg > 2:1024031/1‑148 (MQ=255) tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt < 1:3341843/148‑1 (MQ=255) tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt > 1:2543361/1‑148 (MQ=255) tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt > 1:2543253/1‑148 (MQ=255) aCGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttt > 2:1602121/1‑148 (MQ=255) aCGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttt > 2:1601543/1‑148 (MQ=255) ttATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGtttttt > 2:3192008/1‑148 (MQ=255) tCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTgta > 1:1238793/1‑146 (MQ=255) cGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGaaa < 1:3366138/148‑1 (MQ=255) gCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACg > 2:3358809/1‑148 (MQ=255) aaaTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGa < 1:2102324/148‑1 (MQ=255) aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc < 2:1999583/148‑1 (MQ=255) aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGTAc > 1:517081/1‑148 (MQ=255) ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGTACa > 1:2249251/1‑148 (MQ=255) ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa < 2:2397346/148‑1 (MQ=255) ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa < 2:663870/148‑1 (MQ=255) ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa < 2:607210/148‑1 (MQ=255) ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa > 1:2934826/1‑148 (MQ=255) tGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt < 2:2538470/148‑1 (MQ=255) gCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATc < 2:3202008/148‑1 (MQ=255) cAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcg < 2:1239981/148‑1 (MQ=255) ggCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcgcg > 1:1775604/1‑148 (MQ=255) gAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAgg < 1:1598554/148‑1 (MQ=255) aGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTg < 1:1313917/148‑1 (MQ=255) tAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGa < 1:893028/148‑1 (MQ=255) aGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGaa < 1:2730793/148‑1 (MQ=255) gggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGc > 2:1841834/1‑148 (MQ=255) gggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACATAGCGACGCAGGTGGTAGc > 1:2598391/1‑148 (MQ=255) ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCg > 2:1855279/1‑148 (MQ=255) ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTAGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAAACGAAAAAAGCGACGCAGGTGGAAGCg > 1:1570584/1‑148 (MQ=255) aaGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGAt > 2:176776/1‑148 (MQ=255) gTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACATCGCGACGCAGGTGGTAGCGATAg > 2:2721430/1‑148 (MQ=255) ccGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCgcgc > 1:3183240/1‑148 (MQ=255) cGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGcc > 1:2676170/1‑148 (MQ=255) ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt < 2:814910/148‑1 (MQ=255) ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt < 2:1614904/148‑1 (MQ=255) ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt < 2:3383501/148‑1 (MQ=255) ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt < 1:1167425/148‑1 (MQ=255) ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt < 2:2601189/148‑1 (MQ=255) gTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCg < 1:391600/148‑1 (MQ=255) tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAg > 2:2751043/1‑148 (MQ=255) aCGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTc > 2:1211549/1‑148 (MQ=255) ggTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCa > 2:2547052/1‑148 (MQ=255) ggTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACCCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCa > 1:437722/1‑148 (MQ=255) tgttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCt < 1:1602071/148‑1 (MQ=255) tgttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCt < 2:3373369/148‑1 (MQ=255) gttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCtt < 1:2790234/148‑1 (MQ=255) gTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCa < 2:946995/148‑1 (MQ=255) gTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCa < 2:2469505/148‑1 (MQ=255) tCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCaa > 1:2355046/1‑148 (MQ=255) cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg > 2:1056940/1‑148 (MQ=255) cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg < 1:1481565/148‑1 (MQ=255) cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg < 2:1115595/148‑1 (MQ=255) aaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTc > 1:877926/1‑148 (MQ=255) aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc < 1:2370903/148‑1 (MQ=255) aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc < 1:3525797/148‑1 (MQ=255) ttCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCgg < 2:1428836/148‑1 (MQ=255) tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGc > 2:720675/1‑148 (MQ=255) tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCCGGTGGAAGCGCTAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGc > 2:720770/1‑148 (MQ=255) gCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCAt < 2:3108679/148‑1 (MQ=255) gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg < 2:3273932/148‑1 (MQ=255) gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg > 1:1741446/1‑148 (MQ=255) gTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGAt > 2:2321607/1‑148 (MQ=255) aaaCACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGCCGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAg > 2:1240228/1‑148 (MQ=255) aaCACCACATAGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAgg < 1:2858449/148‑1 (MQ=255) caccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta < 2:2267122/148‑1 (MQ=255) caccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta > 2:2494868/1‑148 (MQ=255) cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAACGAACAAAGCGACCCAGGTGGAAGCGCTAGGTCGCGCCGTTTTCGAGTAATTACCAGCATTCGGCAAGTTCCCGCATCACGCGATAGGTATAg > 1:2133652/1‑148 (MQ=255) cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAg < 2:966832/148‑1 (MQ=255) cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTAGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAg > 1:1692910/1‑148 (MQ=255) acaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAgg < 1:1024031/148‑1 (MQ=255) caTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAggg < 1:3481361/148‑1 (MQ=255) caTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAggg < 1:1717696/148‑1 (MQ=255) tcgtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAt > 2:494197/1‑148 (MQ=255) gtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCg < 1:1441587/148‑1 (MQ=255) cgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGAc < 1:2067993/148‑1 (MQ=255) gCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAGGGATCGACAt > 2:2753939/1‑148 (MQ=255) cattcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATc > 2:2431026/1‑148 (MQ=255) attcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAAAGCGACGCAGGTGGAATCGATAGGTCGCGCCGGTGTCGAGTAATTACCAGCATTAGGCAAGTTACGGCATCAGGCGATAGGTATATGGATCGAAATcc > 1:2554179/1‑148 (MQ=255) ttcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACAAAGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACATACCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATATGGATCGACATCca > 1:2732702/1‑148 (MQ=255) ttcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCca > 2:2902165/1‑148 (MQ=255) tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACGCAGGTGGAATCGAAAGGTCGCGCCGTTTTCGAGTAAATCCCACCTTTAGGCAACTTACGGCAACACGCCATAGGTATAGGGATAGACAACcac > 1:730576/1‑147 (MQ=255) tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcag > 2:1592851/1‑148 (MQ=255) cattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcagc < 1:811184/148‑1 (MQ=255) ttttACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTCTAGGGATCGACATCcagcag > 2:2715719/1‑148 (MQ=255) ttttACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcagcag > 2:1078861/1‑148 (MQ=255) | CCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGT > CP009273/1350878‑1351164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |