Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 119,536 G→T 91.8% P11P (CCG→CCT)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273119,5360GT91.8% 576.2 / 46.0 219P11P (CCG→CCT) aceEpyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (8/10);  new base T (104/97);  total (112/107)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 219 total aligned reads displayed.

CATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAACT  >  CP009273/119390‑119681
                                                                                                                                                  |                                                                                                                                                 
   ataAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCg                                                                                                                                               >  1:2279127/1‑148 (MQ=255)
     aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                             <  2:2301966/148‑1 (MQ=255)
     aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                             >  2:1363126/1‑148 (MQ=255)
     aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                             <  1:2263836/148‑1 (MQ=255)
     aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                             >  2:698816/1‑148 (MQ=255)
     aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                             <  1:796243/148‑1 (MQ=255)
      aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaaa                                                                                                                                            >  1:2697694/1‑148 (MQ=255)
        aGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACt                                                                                                                                          >  2:1095575/1‑148 (MQ=255)
         gcgcGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTc                                                                                                                                         >  1:1625148/1‑148 (MQ=255)
             ggCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGa                                                                                                                                     <  1:317586/148‑1 (MQ=255)
             ggCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGa                                                                                                                                     <  2:3006789/148‑1 (MQ=255)
             ggCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGa                                                                                                                                     <  2:1229084/148‑1 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTgg                                                                                                                                 >  1:1350165/1‑148 (MQ=255)
                       cGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAg                                                                                                                           >  1:836589/1‑148 (MQ=255)
                         tAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGc                                                                                                                         >  2:142793/1‑148 (MQ=255)
                          aGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCg                                                                                                                        >  1:3349067/1‑148 (MQ=255)
                          aGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATCAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCg                                                                                                                        >  1:461589/1‑148 (MQ=255)
                           gAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGa                                                                                                                       <  2:1790315/148‑1 (MQ=255)
                           gAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGa                                                                                                                       >  1:2549683/1‑148 (MQ=255)
                             aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATc                                                                                                                     >  2:3202209/1‑148 (MQ=255)
                             aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATc                                                                                                                     <  2:1747618/148‑1 (MQ=255)
                               cTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                                                                                   >  2:1901105/1‑148 (MQ=255)
                                tGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGaa                                                                                                                  <  2:3398342/148‑1 (MQ=255)
                                 gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAAt                                                                                                                 <  2:2114554/148‑1 (MQ=255)
                                  tCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATc                                                                                                                >  2:3170516/1‑148 (MQ=255)
                                  tCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATc                                                                                                                >  2:399620/1‑148 (MQ=255)
                                    ttATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCgg                                                                                                              <  2:886272/148‑1 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCTATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                                                                                             >  2:3409439/1‑148 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                                                                                             <  1:3099809/148‑1 (MQ=255)
                                       ttGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCa                                                                                                           <  2:1753429/148‑1 (MQ=255)
                                          aGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATcc                                                                                                        >  1:971026/1‑148 (MQ=255)
                                           gCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCg                                                                                                       <  1:2912664/148‑1 (MQ=255)
                                           gCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCg                                                                                                       >  1:1215054/1‑148 (MQ=255)
                                           gCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCg                                                                                                       >  1:1215992/1‑148 (MQ=255)
                                           gCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCg                                                                                                       <  1:2175996/148‑1 (MQ=255)
                                            cTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGt                                                                                                      >  2:3323261/1‑148 (MQ=255)
                                               tCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaa                                                                                                   <  1:704134/148‑1 (MQ=255)
                                               tCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaa                                                                                                   >  1:3213667/1‑148 (MQ=255)
                                                  ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaagaa                                                                                                <  2:2399161/148‑1 (MQ=255)
                                                      agagTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgtg                                                                                            <  1:2136226/148‑1 (MQ=255)
                                                        agTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt                                                                                          >  2:2825314/1‑148 (MQ=255)
                                                         gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTg                                                                                         <  2:2849531/148‑1 (MQ=255)
                                                         gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTg                                                                                         <  2:2849580/148‑1 (MQ=255)
                                                           tCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAg                                                                                       <  2:2394593/148‑1 (MQ=255)
                                                           tCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAg                                                                                       <  2:2386097/148‑1 (MQ=255)
                                                              aTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGt                                                                                    >  1:2658916/1‑148 (MQ=255)
                                                                gggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGc                                                                                  >  2:35254/1‑148 (MQ=255)
                                                                      ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTa                                                                            >  2:2097977/1‑148 (MQ=255)
                                                                       gTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTAt                                                                           <  2:521090/148‑1 (MQ=255)
                                                                        ttCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATc                                                                          >  1:607231/1‑148 (MQ=255)
                                                                        ttCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATc                                                                          <  1:1406236/148‑1 (MQ=255)
                                                                          ccAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTg                                                                        >  1:2403920/1‑148 (MQ=255)
                                                                            aGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt                                                                      >  2:1998426/1‑148 (MQ=255)
                                                                            aGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt                                                                      <  1:2946448/148‑1 (MQ=255)
                                                                             gAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc                                                                     >  2:3451694/1‑148 (MQ=255)
                                                                              aaaaCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCg                                                                    >  2:2462908/1‑148 (MQ=255)
                                                                                   tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCaa                                                               <  1:1168664/148‑1 (MQ=255)
                                                                                   tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCaa                                                               <  1:3506785/148‑1 (MQ=255)
                                                                                   tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCaa                                                               >  1:34508/1‑148 (MQ=255)
                                                                                       cGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgc                                                           >  1:1185977/1‑148 (MQ=255)
                                                                                        gTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgct                                                          <  1:2231906/148‑1 (MQ=255)
                                                                                         ttattaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgctt                                                         >  1:672073/1‑148 (MQ=255)
                                                                                          tattaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttg                                                        <  2:49085/148‑1 (MQ=255)
                                                                                                atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAg                                                  >  1:2704946/1‑148 (MQ=255)
                                                                                                    ataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCg                                              >  2:2529632/1‑148 (MQ=255)
                                                                                                        ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCaaa                                          >  2:724716/1‑148 (MQ=255)
                                                                                                          aaTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAgg                                        >  2:2325755/1‑148 (MQ=255)
                                                                                                           aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGc                                       >  1:3419451/1‑148 (MQ=255)
                                                                                                            tAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGTCg                                      >  1:2350220/1‑148 (MQ=255)
                                                                                                               cccATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                   <  2:1049544/148‑1 (MQ=255)
                                                                                                               cccATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                   <  1:151918/148‑1 (MQ=255)
                                                                                                               cccATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                   >  2:312892/1‑148 (MQ=255)
                                                                                                                   tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                               <  1:2156652/148‑1 (MQ=255)
                                                                                                                   tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                               <  1:380077/148‑1 (MQ=255)
                                                                                                                   tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                               <  1:1022204/148‑1 (MQ=255)
                                                                                                                     tCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACg                             >  2:2779258/1‑148 (MQ=255)
                                                                                                                       aGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTa                           <  2:1215979/148‑1 (MQ=255)
                                                                                                                       aGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTa                           <  1:2199831/148‑1 (MQ=255)
                                                                                                                         aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTatc                         >  2:2376221/1‑146 (MQ=255)
                                                                                                                         aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                         >  1:2144590/1‑148 (MQ=255)
                                                                                                                          aCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGcc                        >  2:1983926/1‑148 (MQ=255)
                                                                                                                            gTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGc                      <  2:836589/148‑1 (MQ=255)
                                                                                                                              ttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg                    >  2:919353/1‑148 (MQ=255)
                                                                                                                              ttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg                    >  2:666810/1‑148 (MQ=255)
                                                                                                                              ttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg                    >  1:2589374/1‑148 (MQ=255)
                                                                                                                                cccAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGc                  >  2:2976546/1‑148 (MQ=255)
                                                                                                                                cccAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGc                  >  1:1908207/1‑148 (MQ=255)
                                                                                                                                  cAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGcac                >  1:289581/1‑148 (MQ=255)
                                                                                                                                  cAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGcac                <  2:2144063/148‑1 (MQ=255)
                                                                                                                                    aaTGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAg              <  2:2704946/148‑1 (MQ=255)
                                                                                                                                        aCGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTAt          <  1:1998426/148‑1 (MQ=255)
                                                                                                                                         cGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATc         <  1:1363126/148‑1 (MQ=255)
                                                                                                                                            ggATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGc      >  2:101024/1‑148 (MQ=255)
                                                                                                                                            ggATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGc      <  2:1210430/148‑1 (MQ=255)
                                                                                                                                            ggATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGc      >  2:1823215/1‑148 (MQ=255)
                                                                                                                                              aTCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCaa    <  1:35254/148‑1 (MQ=255)
                                                                                                                                              aTCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCaa    >  2:3376808/1‑148 (MQ=255)
                                                                                                                                              aTCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCaa    >  2:2351489/1‑148 (MQ=255)
                                                                                                                                                ccGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAACt  <  2:461589/148‑1 (MQ=255)
                                                                                                                                                ccGATCGAAACTCGCGACTGGCTCCAGGAGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAACt  <  2:3349067/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAACT  >  CP009273/119390‑119681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑