Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,365,283 G→T 100% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,365,2830GT99.2% 479.5 / ‑5.6 179E271* (GAG→TAG) ycjMalpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base T (98/74);  minor base A (5/0);  total (104/75)
Fisher's exact test for biased strand distribution p-value = 7.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365137‑1365426
                                                                                                                                                  |                                                                                                                                               
gTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCga                                                                                                                                                >  1:1590000/1‑148 (MQ=255)
 tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtag                                                                                                                                               <  1:1796695/148‑1 (MQ=255)
 tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtag                                                                                                                                               <  1:1796694/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGTAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACTCATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                              <  2:2793321/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                              <  2:3120732/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCCGGTACAGTGATAATTACCtaga                                                                                                                                              >  2:1870440/1‑148 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACtaga                                                                                                                                              >  1:3330312/1‑148 (MQ=255)
     gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                           >  2:2520145/1‑148 (MQ=255)
       gTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                         <  1:1744851/148‑1 (MQ=255)
        tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATTAAGTTTGGCCAGGTAAAGTGATAAATAGCTAGAGCAAtt                                                                                                                                        >  1:1518521/1‑147 (MQ=255)
        tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                        >  2:664740/1‑148 (MQ=255)
         aTGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGt                                                                                                                                       >  1:2215466/1‑148 (MQ=255)
          tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGtt                                                                                                                                      <  1:2969371/148‑1 (MQ=255)
           gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTc                                                                                                                                     >  1:1404353/1‑148 (MQ=255)
              cGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCCATGTTACGc                                                                                                                                  >  1:618163/1‑148 (MQ=255)
                    gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                            <  2:67304/148‑1 (MQ=255)
                    gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                            <  1:815541/148‑1 (MQ=255)
                     aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGa                                                                                                                           <  2:2068357/148‑1 (MQ=255)
                      tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                          <  2:2641012/148‑1 (MQ=255)
                       gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                         >  2:3013414/1‑148 (MQ=255)
                       gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                         <  1:51066/148‑1 (MQ=255)
                         cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGGTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaac                                                                                                                       <  1:3060116/148‑1 (MQ=255)
                          gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaaca                                                                                                                      >  2:2180882/1‑148 (MQ=255)
                           ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAt                                                                                                                     <  2:1361790/148‑1 (MQ=255)
                               cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCt                                                                                                                 <  2:1552455/148‑1 (MQ=255)
                               cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCt                                                                                                                 <  2:1552861/148‑1 (MQ=255)
                                tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCtt                                                                                                                >  1:1182977/1‑148 (MQ=255)
                                tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCtt                                                                                                                >  1:659598/1‑148 (MQ=255)
                                 ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTa                                                                                                               <  1:1072393/148‑1 (MQ=255)
                                   aTGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAATACAACAATGCTTACt                                                                                                             >  1:2006190/1‑148 (MQ=255)
                                     gtgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACttt                                                                                                           <  1:2988117/148‑1 (MQ=255)
                                      tgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTg                                                                                                          <  2:3479754/148‑1 (MQ=255)
                                       ggAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTgg                                                                                                         >  2:2435042/1‑148 (MQ=255)
                                        gAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgc                                                                                                        >  1:848515/1‑148 (MQ=255)
                                         aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTACGCCTAAAGACAACAATGCTTACTTTGgcg                                                                                                       >  1:75418/1‑148 (MQ=255)
                                          aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACCTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgcgc                                                                                                      >  2:1380651/1‑148 (MQ=255)
                                           agagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAAATTGCTTACTTTTGCGCa                                                                                                     >  1:1369575/1‑148 (MQ=255)
                                            gagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                    >  2:3282542/1‑148 (MQ=255)
                                             agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAAAACAATTCTTAATTTTGCGCAgg                                                                                                   >  1:1018038/1‑148 (MQ=255)
                                               ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCg                                                                                                 <  2:2970381/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:3330305/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:844860/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:968614/148‑1 (MQ=255)
                                                       aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGaa                                                                                         <  2:2635260/148‑1 (MQ=255)
                                                        aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                        >  1:2646699/1‑148 (MQ=255)
                                                         gCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                       <  2:2279067/148‑1 (MQ=255)
                                                           tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGcac                                                                                     <  1:998250/148‑1 (MQ=255)
                                                            gCATACATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACTGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAATTTGTCGCAGGCGATGACTAAGcaca                                                                                    <  2:615316/148‑1 (MQ=255)
                                                               tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACatat                                                                                 >  2:2749971/1‑148 (MQ=255)
                                                               tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATTTTCCGCATAAAGAAAACATTGCTTAATTTGGCGCATGCGATTACGAAGCACatat                                                                                 >  1:2774519/1‑148 (MQ=255)
                                                                ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTACGCAAAAAGACAACATTGCTTACTTTGGCGCCGGCGATGACGAAGCACATATg                                                                                >  1:1661085/1‑148 (MQ=255)
                                                                 catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATgg                                                                               <  1:422243/148‑1 (MQ=255)
                                                                   tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGAAAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGgtg                                                                             >  2:351511/1‑148 (MQ=255)
                                                                   tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTAAAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtg                                                                             >  2:437402/1‑148 (MQ=255)
                                                                      ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAc                                                                          >  1:2084979/1‑148 (MQ=255)
                                                                      ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACCTATGGTGTAc                                                                          >  2:3101591/1‑148 (MQ=255)
                                                                         aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAg                                                                       >  2:2316344/1‑148 (MQ=255)
                                                                          aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                      <  2:1206288/148‑1 (MQ=255)
                                                                               acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                 >  2:2913600/1‑148 (MQ=255)
                                                                               acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                 <  2:610775/148‑1 (MQ=255)
                                                                                   cTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgc                                                             <  1:2407370/148‑1 (MQ=255)
                                                                                   cTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGGGATCATTAACAAGAACAATTTTACGCATAAAGACAACATTGCTTACTTTGGCGCCGGCGCTGACGAAGCACATATGGTGTACCCGTTCTCGCTgc                                                             >  2:2565700/1‑148 (MQ=255)
                                                                                     gATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccg                                                           >  2:2336806/1‑148 (MQ=255)
                                                                                      aTTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                          <  2:796713/148‑1 (MQ=255)
                                                                                       ttATCAAACTGTTACGGTCGAGTATTTATAAAGTTTCGCCAGGTACAGTGATAATTACCAAGAACAAAGTTACGCATAAAGACAAAAATTCTTACTTTTGCGCAGGCGATGACGAAGCACAAAAGGTGTACCAGTTCTAGCAgccccc                                                         >  1:2712004/1‑148 (MQ=255)
                                                                                        tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa                                                        <  1:2305117/148‑1 (MQ=255)
                                                                                          tCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACt                                                      >  1:823950/1‑148 (MQ=255)
                                                                                             aaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                   >  2:1260071/1‑148 (MQ=255)
                                                                                              aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCAAGACCAATTTTACGCATAAATACAACAATTCTTAATTTGGCGCAGGCGATGACGAAGCACATATTGTGTAACAGTTCTCGCTGCCGCCACAGGtg                                                  >  1:451070/1‑148 (MQ=255)
                                                                                                tGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct                                                >  2:147582/1‑148 (MQ=255)
                                                                                                  ttACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATTTTACGCATAAAGACAACAATTCTTACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTACCAGTTCTAGCTGCCGCCACAGGtgctgc                                              >  2:911309/1‑148 (MQ=255)
                                                                                                    aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCTGGCGATGACGAAGCACATTTGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                            <  2:2699490/148‑1 (MQ=255)
                                                                                                    aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                            <  2:887961/148‑1 (MQ=255)
                                                                                                     cGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGGTCTCGCTGCCGCCACTGGTGCTGCATg                                           >  2:600467/1‑148 (MQ=255)
                                                                                                                 gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                               <  1:3249824/148‑1 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:2384516/1‑148 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:1627260/1‑148 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              <  2:133996/148‑1 (MQ=255)
                                                                                                                    aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa                            <  1:120093/148‑1 (MQ=255)
                                                                                                                      cGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                          <  1:1307943/148‑1 (MQ=255)
                                                                                                                        ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                        >  1:1117749/1‑148 (MQ=255)
                                                                                                                        ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                        >  1:2895086/1‑148 (MQ=255)
                                                                                                                         tGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                       >  1:2521354/1‑148 (MQ=255)
                                                                                                                          gcgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTg                      >  2:3501591/1‑148 (MQ=255)
                                                                                                                            gcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAg                    <  2:848515/148‑1 (MQ=255)
                                                                                                                              cAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgc                  >  1:856008/1‑148 (MQ=255)
                                                                                                                                ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc                >  1:1465528/1‑148 (MQ=255)
                                                                                                                                 gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               >  2:394028/1‑148 (MQ=255)
                                                                                                                                 gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               <  1:1260071/148‑1 (MQ=255)
                                                                                                                                    cAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg            <  1:665104/148‑1 (MQ=255)
                                                                                                                                       tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGc         >  1:2140446/1‑148 (MQ=255)
                                                                                                                                       tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGc         >  2:3142578/1‑148 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:743098/1‑148 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        <  2:1590000/148‑1 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:1150127/1‑148 (MQ=255)
                                                                                                                                           cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg     >  1:2832504/1‑148 (MQ=255)
                                                                                                                                             ttACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc   >  1:2804652/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  2:2160180/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  1:903956/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  1:1974769/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365137‑1365426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑