Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 10,444 G→T 100% F17L (TTC→TTA)  satP ← succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927310,4440GT99.1% 743.1 / ‑1.9 227F17L (TTC→TTA) satPsuccinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (105/120);  total (106/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 227 total aligned reads displayed.

ACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGG  >  CP009273/10300‑10588
                                                                                                                                                |                                                                                                                                                
 ccGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                              >  1:3525531/1‑148 (MQ=255)
 ccGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                              >  2:3420523/1‑148 (MQ=255)
  cGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGcc                                                                                                                                             >  1:1451705/1‑148 (MQ=255)
  cGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGcc                                                                                                                                             <  2:1946824/148‑1 (MQ=255)
  cGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGcc                                                                                                                                             <  2:2776232/148‑1 (MQ=255)
     aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                          >  1:2207760/1‑148 (MQ=255)
     aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                          >  1:1518892/1‑148 (MQ=255)
     aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                          >  2:3194530/1‑148 (MQ=255)
      aGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATc                                                                                                                                         <  2:489036/148‑1 (MQ=255)
      aGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATc                                                                                                                                         >  1:495392/1‑148 (MQ=255)
           tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                    <  1:1069744/148‑1 (MQ=255)
           tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                    <  1:1263070/148‑1 (MQ=255)
             ccTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCa                                                                                                                                  >  2:3093281/1‑148 (MQ=255)
                 ttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCgg                                                                                                                              >  2:3273764/1‑148 (MQ=255)
                    tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGc                                                                                                                           <  2:3415850/148‑1 (MQ=255)
                     gTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGcc                                                                                                                          >  2:3428483/1‑148 (MQ=255)
                       aCTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCgg                                                                                                                        <  2:1004873/148‑1 (MQ=255)
                         tCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAt                                                                                                                      <  2:2543908/148‑1 (MQ=255)
                             gcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATAAGGCCCAGCGGTGCCGGATTAGc                                                                                                                  <  1:270829/148‑1 (MQ=255)
                                 aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                              >  1:3347303/1‑148 (MQ=255)
                                      cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgt                                                                                                         >  2:2365637/1‑148 (MQ=255)
                                      cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgt                                                                                                         <  1:686736/148‑1 (MQ=255)
                                       aaaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                                                                                                        <  1:784797/148‑1 (MQ=255)
                                        aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtgt                                                                                                       <  2:1027930/148‑1 (MQ=255)
                                           aTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGc                                                                                                    >  2:1557273/1‑148 (MQ=255)
                                            tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                   >  2:3177209/1‑148 (MQ=255)
                                            tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                   >  1:81115/1‑148 (MQ=255)
                                            tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                   >  1:1420338/1‑148 (MQ=255)
                                               gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt                                                                                                <  1:2797620/148‑1 (MQ=255)
                                               gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt                                                                                                <  2:82324/148‑1 (MQ=255)
                                               gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt                                                                                                >  2:2303361/1‑148 (MQ=255)
                                                 gcgATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATaa                                                                                              <  1:44905/148‑1 (MQ=255)
                                                    aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTc                                                                                           <  2:939692/148‑1 (MQ=255)
                                                    aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTc                                                                                           <  1:1709646/148‑1 (MQ=255)
                                                      gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                         >  2:1764297/1‑148 (MQ=255)
                                                      gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                         <  2:2892769/148‑1 (MQ=255)
                                                      gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                         >  2:430410/1‑148 (MQ=255)
                                                      gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                         <  1:427296/148‑1 (MQ=255)
                                                            gTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaaa                                                                                   >  2:696325/1‑148 (MQ=255)
                                                             tAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAat                                                                                  <  2:2929853/148‑1 (MQ=255)
                                                              aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                 >  1:2641091/1‑148 (MQ=255)
                                                                aaaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcat                                                                               >  2:3461508/1‑148 (MQ=255)
                                                                 aaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatc                                                                              <  1:2365637/148‑1 (MQ=255)
                                                                 aaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatc                                                                              <  1:3454447/148‑1 (MQ=255)
                                                                   aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                            <  1:2303361/148‑1 (MQ=255)
                                                                     gCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCg                                                                          >  1:901722/1‑148 (MQ=255)
                                                                       ccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                        <  2:606151/148‑1 (MQ=255)
                                                                          tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                     <  1:512025/148‑1 (MQ=255)
                                                                          tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                     <  1:206414/148‑1 (MQ=255)
                                                                           ggCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaa                                                                    <  1:151419/148‑1 (MQ=255)
                                                                            gCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                   <  2:495392/148‑1 (MQ=255)
                                                                            gCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                   >  2:2475690/1‑148 (MQ=255)
                                                                               aGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGt                                                                <  2:3296738/148‑1 (MQ=255)
                                                                                 aataatACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGa                                                              >  2:3343478/1‑148 (MQ=255)
                                                                                  ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaa                                                             <  2:1901493/148‑1 (MQ=255)
                                                                                  ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATAGAATGAATGGTGaa                                                             >  1:415796/1‑148 (MQ=255)
                                                                                   taataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaaa                                                            >  2:3540301/1‑148 (MQ=255)
                                                                                   taataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaaa                                                            >  1:2142149/1‑148 (MQ=255)
                                                                                       aCCGTCCAGAGCTAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataat                                                        >  2:3405488/1‑148 (MQ=255)
                                                                                           tCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTcc                                                    <  2:2133094/148‑1 (MQ=255)
                                                                                              agagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTg                                                 >  1:3420510/1‑148 (MQ=255)
                                                                                              agagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTg                                                 >  1:2100854/1‑148 (MQ=255)
                                                                                               gagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                                <  2:3050071/148‑1 (MQ=255)
                                                                                               gagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                                <  1:1017408/148‑1 (MQ=255)
                                                                                                agCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGaa                                               >  1:1681209/1‑148 (MQ=255)
                                                                                                 gCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAAt                                              <  1:198300/148‑1 (MQ=255)
                                                                                                  cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATa                                             >  1:3182596/1‑148 (MQ=255)
                                                                                                    aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                           >  2:2054068/1‑148 (MQ=255)
                                                                                                    aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                           <  2:1051324/148‑1 (MQ=255)
                                                                                                    aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                           <  1:474419/148‑1 (MQ=255)
                                                                                                     aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                          <  1:641985/148‑1 (MQ=255)
                                                                                                        aaCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTa                                       <  1:3208758/148‑1 (MQ=255)
                                                                                                          cccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgt                                     >  1:250243/1‑148 (MQ=255)
                                                                                                          cccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgt                                     >  2:648845/1‑148 (MQ=255)
                                                                                                            cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                   <  2:1284997/148‑1 (MQ=255)
                                                                                                               gTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtttt                                <  1:295038/148‑1 (MQ=255)
                                                                                                                ttGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTc                               <  2:2348154/148‑1 (MQ=255)
                                                                                                                 tgtgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCa                              <  1:2902027/148‑1 (MQ=255)
                                                                                                                 tgtgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCa                              <  1:2546589/148‑1 (MQ=255)
                                                                                                                        gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGcgcg                       >  1:844760/1‑148 (MQ=255)
                                                                                                                          tAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGc                     >  2:3228833/1‑148 (MQ=255)
                                                                                                                            aGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                   <  1:2371974/148‑1 (MQ=255)
                                                                                                                            aGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                   <  1:582193/148‑1 (MQ=255)
                                                                                                                             gCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCata                  <  2:2521343/148‑1 (MQ=255)
                                                                                                                              cAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataa                 <  2:1578241/148‑1 (MQ=255)
                                                                                                                              cAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACATAGTGTTGCCCATAATTCCTCAAAAATCATCCTCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataa                 <  2:3346030/148‑1 (MQ=255)
                                                                                                                                gAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataata               <  1:3273764/148‑1 (MQ=255)
                                                                                                                                 aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa              >  1:2485174/1‑148 (MQ=255)
                                                                                                                                 aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa              >  2:1994889/1‑148 (MQ=255)
                                                                                                                                    ggtggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCa           >  1:1792715/1‑148 (MQ=255)
                                                                                                                                     gtggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAg          <  1:1557273/148‑1 (MQ=255)
                                                                                                                                       ggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGcc        >  1:2028648/1‑148 (MQ=255)
                                                                                                                                         tCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAg      <  1:2542814/148‑1 (MQ=255)
                                                                                                                                         tCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAGAATAATCAGCCAg      >  2:1359693/1‑148 (MQ=255)
                                                                                                                                          cATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGt     >  1:1189286/1‑148 (MQ=255)
                                                                                                                                           aTGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTg    <  1:3289253/148‑1 (MQ=255)
                                                                                                                                            tGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATGATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTgg   >  2:2075302/1‑148 (MQ=255)
                                                                                                                                            tGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTgg   >  2:168471/1‑148 (MQ=255)
                                                                                                                                             gCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTggg  <  1:3093281/148‑1 (MQ=255)
                                                                                                                                             gCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTggg  >  1:2599395/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGG  >  CP009273/10300‑10588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑