Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 135,689 A→T 60.7% L675Q (CTG→CAG)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273135,6890AT60.7% 160.7 / 250.4 206L675Q (CTG→CAG) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (41/40);  new base T (56/69);  total (97/109)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 206 total aligned reads displayed.

CAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCT  >  CP009273/135544‑135833
                                                                                                                                                 |                                                                                                                                                
cAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgc                                                                                                                                                >  2:1894402/1‑148 (MQ=255)
cAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgc                                                                                                                                                >  2:3553167/1‑148 (MQ=255)
  gCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTgcgc                                                                                                                                              <  1:1960265/148‑1 (MQ=255)
  gCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgcgc                                                                                                                                              <  2:909600/148‑1 (MQ=255)
       ttACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCgaga                                                                                                                                         <  2:1059676/148‑1 (MQ=255)
       ttACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCgaga                                                                                                                                         <  1:2215718/148‑1 (MQ=255)
        tACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGat                                                                                                                                        >  1:1252127/1‑148 (MQ=255)
        tACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGat                                                                                                                                        <  2:2755439/148‑1 (MQ=255)
           ccgccgTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGAtata                                                                                                                                     <  2:3424529/148‑1 (MQ=255)
             gccgTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAAc                                                                                                                                   <  1:1412364/148‑1 (MQ=255)
                gTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAAcccc                                                                                                                                <  1:280825/148‑1 (MQ=255)
                gTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAAcccc                                                                                                                                <  2:2147664/148‑1 (MQ=255)
                gTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAAcccc                                                                                                                                <  1:10182/148‑1 (MQ=255)
                   gagaTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATg                                                                                                                             >  2:1179760/1‑148 (MQ=255)
                    agaTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgc                                                                                                                            <  1:1003710/148‑1 (MQ=255)
                     gaTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgct                                                                                                                           <  1:3410633/148‑1 (MQ=255)
                      aTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctg                                                                                                                          >  1:2926073/1‑148 (MQ=255)
                       ttGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctgg                                                                                                                         >  1:3265423/1‑148 (MQ=255)
                       ttGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctgg                                                                                                                         >  1:493609/1‑148 (MQ=255)
                        tGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctggc                                                                                                                        <  2:3507369/148‑1 (MQ=255)
                          gcccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctggctg                                                                                                                      <  2:3476197/148‑1 (MQ=255)
                           cccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGt                                                                                                                     <  2:879124/148‑1 (MQ=255)
                           cccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGt                                                                                                                     <  1:1844689/148‑1 (MQ=255)
                           cccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGt                                                                                                                     <  2:2203655/148‑1 (MQ=255)
                           cccgcccAGCATCGGCATACCCATATTGAACAGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGt                                                                                                                     >  2:2973314/1‑148 (MQ=255)
                            ccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGtt                                                                                                                    <  1:2844436/148‑1 (MQ=255)
                            ccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGtt                                                                                                                    <  2:730212/148‑1 (MQ=255)
                               cccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTAc                                                                                                                 <  1:1201948/148‑1 (MQ=255)
                               cccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTAc                                                                                                                 <  1:3529646/148‑1 (MQ=255)
                                 cAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACAt                                                                                                               >  1:678909/1‑148 (MQ=255)
                                    cATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGc                                                                                                            >  1:1488166/1‑148 (MQ=255)
                                    cATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGc                                                                                                            >  1:1156767/1‑148 (MQ=255)
                                     aTCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAAGACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCa                                                                                                           <  2:923081/148‑1 (MQ=255)
                                      tCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAg                                                                                                          >  2:2584693/1‑148 (MQ=255)
                                           aTACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCaa                                                                                                     <  2:201356/148‑1 (MQ=255)
                                             aCCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAAt                                                                                                   <  2:78615/148‑1 (MQ=255)
                                              cccATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATg                                                                                                  >  2:2113281/1‑148 (MQ=255)
                                              cccATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATg                                                                                                  <  1:2671466/148‑1 (MQ=255)
                                               ccATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                                                                                                 <  1:1894402/148‑1 (MQ=255)
                                               ccATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                                                                                                 >  2:313212/1‑148 (MQ=255)
                                               caatatTTAACGGCACCGGAACCGGCATCGGGATCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTAAGTTCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                                                                                                 <  1:215116/146‑1 (MQ=255)
                                                  tatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTga                                                                                              <  2:2662343/148‑1 (MQ=255)
                                                     tGAACGGCACCGGCACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGagag                                                                                           <  2:1721482/148‑1 (MQ=255)
                                                     tGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGagag                                                                                           <  2:1720963/148‑1 (MQ=255)
                                                        aCGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGaa                                                                                        <  1:1990396/148‑1 (MQ=255)
                                                           gCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACgg                                                                                     <  1:2440559/148‑1 (MQ=255)
                                                            cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACggg                                                                                    >  1:126655/1‑148 (MQ=255)
                                                            cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACggg                                                                                    <  1:1908842/148‑1 (MQ=255)
                                                             aCCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGt                                                                                   >  1:1709100/1‑148 (MQ=255)
                                                              ccGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGtt                                                                                  >  2:3058445/1‑148 (MQ=255)
                                                                ggAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGa                                                                                <  2:64096/148‑1 (MQ=255)
                                                                 gAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAg                                                                               >  2:2090215/1‑148 (MQ=255)
                                                                 gAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTGTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAg                                                                               <  2:2540345/148‑1 (MQ=255)
                                                                     cGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTg                                                                           >  2:2595143/1‑148 (MQ=255)
                                                                         aTCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGacac                                                                       >  2:1210815/1‑148 (MQ=255)
                                                                            gggAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCAt                                                                    <  2:334462/148‑1 (MQ=255)
                                                                             ggAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATa                                                                   <  1:3047865/148‑1 (MQ=255)
                                                                               aaCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGACCGGGTTGAGCGTGACACCATACg                                                                 >  2:1459182/1‑148 (MQ=255)
                                                                                aCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACgg                                                                <  2:17222/148‑1 (MQ=255)
                                                                                aCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACgg                                                                >  2:1671138/1‑148 (MQ=255)
                                                                                aCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACgg                                                                >  2:2258203/1‑148 (MQ=255)
                                                                                 cGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                               <  1:796294/148‑1 (MQ=255)
                                                                                      tACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACAcc                                                          >  2:76852/1‑148 (MQ=255)
                                                                                      tACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACAcc                                                          >  2:718263/1‑148 (MQ=255)
                                                                                      tACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACAcc                                                          >  2:3349800/1‑148 (MQ=255)
                                                                                           tCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACt                                                     >  1:3436567/1‑148 (MQ=255)
                                                                                            ccTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtg                                                    <  2:2041748/148‑1 (MQ=255)
                                                                                            ccTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtg                                                    <  1:2367154/148‑1 (MQ=255)
                                                                                            ccTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtg                                                    <  2:1252156/148‑1 (MQ=255)
                                                                                               gcggcgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTgg                                                 <  1:3330789/148‑1 (MQ=255)
                                                                                                   cgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGa                                             <  1:2413410/148‑1 (MQ=255)
                                                                                                    gTACCAATACGTTTCGTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGaa                                            <  1:297111/148‑1 (MQ=255)
                                                                                                      aCCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATg                                          <  2:2408398/148‑1 (MQ=255)
                                                                                                      aCCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATg                                          <  2:3190445/148‑1 (MQ=255)
                                                                                                          aTACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCgg                                      >  1:1736571/1‑148 (MQ=255)
                                                                                                           tACTTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGa                                     >  1:1934185/1‑148 (MQ=255)
                                                                                                              gTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTc                                  <  1:1588646/148‑1 (MQ=255)
                                                                                                               tttCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTcc                                 >  2:205656/1‑148 (MQ=255)
                                                                                                               tttCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTcc                                 >  2:1012444/1‑148 (MQ=255)
                                                                                                                ttcttcCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  2:3152515/1‑148 (MQ=255)
                                                                                                                ttcttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  1:3354188/1‑148 (MQ=255)
                                                                                                                ttcttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  1:3460097/1‑148 (MQ=255)
                                                                                                                  cttcCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTa                              <  2:1158428/148‑1 (MQ=255)
                                                                                                                       acacCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGt                         >  2:1437203/1‑148 (MQ=255)
                                                                                                                        caccacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTg                        >  2:3254162/1‑148 (MQ=255)
                                                                                                                          ccacTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGcc                      <  2:827764/148‑1 (MQ=255)
                                                                                                                           cacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCt                     >  1:3512854/1‑148 (MQ=255)
                                                                                                                           cacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGGACCCGTGCCt                     <  2:3388868/148‑1 (MQ=255)
                                                                                                                            acTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCtt                    <  2:2824021/148‑1 (MQ=255)
                                                                                                                                  ttAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCAt              >  2:1980125/1‑148 (MQ=255)
                                                                                                                                   tAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATc             <  1:421650/148‑1 (MQ=255)
                                                                                                                                   tAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATc             <  2:423834/148‑1 (MQ=255)
                                                                                                                                   tAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATc             >  2:3563319/1‑148 (MQ=255)
                                                                                                                                    aGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCt            <  1:2738255/148‑1 (MQ=255)
                                                                                                                                       tttCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTc         <  1:2039952/148‑1 (MQ=255)
                                                                                                                                       tttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTc         <  2:3528612/148‑1 (MQ=255)
                                                                                                                                       tttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTc         >  1:3156492/1‑148 (MQ=255)
                                                                                                                                            gATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    >  1:3525545/1‑148 (MQ=255)
                                                                                                                                            gATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    >  1:3110440/1‑148 (MQ=255)
                                                                                                                                            gATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    >  1:116823/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCT  >  CP009273/135544‑135833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑